Walter E. Washington Convention Center
#cellbio2022
Microsymposia (Poster Teasers)
New for 2022, we have Microsymposia Poster Teaser Sessions prior to the poster presentations in the exhibit hall. Each day, the top-scoring 70 abstracts submitted by the August 2 deadline will present a 3- to 4-minute overview of their research. There are 21 Microsymposia sessions, seven on each day, with 10 poster teasers in each session. The poster teasers are loosely grouped by topic. Following the poster teasers, attendees can head down to the Exhibit Hall to visit the posters and ask the presenters any questions that they may have.
Microsymposia Sessions and Schedule
Sunday, December 4, 11:15 am to 12:05 pm
Track(s): Cellular Dynamics, and Physical Cell
Room 202B
11:15 am MS1 Defining mechanisms of microvilli biogenesis using direct visualization. I. Gaeta1, L. Meenderink2, M. Postema1, C. Cencer1, M. Tyska1 1Vanderbilt University, Nashville, TN, 2Veterans Affairs Tennessee Valley Health Care System, Nashville, TN
11:20 am MS2 An unconventional myosin-based mechanism of vesicle transport in Toxoplasma gondii. J. A. Kellermeier, T. E. Sladewski, Jr, A. T. Heaslip University of Connecticut, Storrs, CT
11:25 am MS3 Determining the role of Plastin-3 in osteoblast mineralization and mechanosensation. S. M. Chin, S. Jansen Washington University in St. Louis, Saint Louis, MO
11:30 am MS4 Arp2/3 complex-nucleated, light-dependent filopodia in amoeboid algae perform bidirectional cyclosis. P. Avasthi, C. D. MacQuarrie Arcadia Science, Berkeley, CA
11:35 am MS5 Using MVID-Causing Mutations to Investigate MYO5B Motor Function. D. M. Bowman1,2, M. J. Tyska1,2, J. R. Goldenring1,2,2 1Vanderbilt University School of Medicine, Nashville, TN, 2Vanderbilt University Medical Center, Nashville, TN
11:40 am MS6 Liquid-like assembly of VASP catalyzes actin polymerization and bundling. K. Graham1, A. Chandrasekaran2, L. Wang3, A. Ladak1, E. Lafer3, P. Rangamani2, J. Stachowiak1 1The University of Texas At Austin, Austin, TX, 2University of California San Diego, La Jolla, CA, 3University of Texas Health Science Center at San Antonio, San Antonio, TX
11:45 am MS7 Bending forces and nucleotide state jointly regulate F-actin structure. M. J. Reynolds, C. Hachicho, A. G. Carl, R. Gong, G. M. Alushin The Rockefeller University, New York, NY
11:50 am MS8 β-actin protein is essential for retinal physiology. P. Vedula, M. E. Fina, B. A. Bell, S. S. Nikonov, A. S. Kashina, D. W. Dong University of Pennsylvania, Philadelphia, PA
11:55 am MS9 An interphase actin wave promotes mitochondrial content mixing to maintain cellular homeostasis. S. M. Coscia1, A. S. Moore2, E. L. F. Holzbaur1 1University of Pennsylvania, Philadelphia, PA, 2Howard Hughes Medical Institute, Ashburn, VA
12:00 pm MS10 Mathematical modeling and biochemical analysis support partially ordered CaM-MLCK binding. M. J. S. MacEwen1, D.-V. Rusnac1, H. Ermias1, J. P. Dexter2, Y. Sancak1 1University of Washington, Seattle, WA, 2Harvard University, Cambridge, MA
Track(s): Cells in Distress; and Disease Communal Cell
Room 145B
11:15 am MS11 CAR-Macrophages reprogram neighboring macrophages for efficient cancer cell phagocytosis. T. Waddell, Q. Xue, M. Roh-Johnson University of Utah, Salt Lake City, UT
11:20 am MS12 Evaluating drug response in 3D triple negative breast cancer organoids with high content imaging and analysis. O. Sirenko Molecular Devices, San Jose, CA
11:25 am MS13 p27 Increases Breast Cancer Stem Cell (CSC) Expansion by Recruiting STAT3 and CBP on Chromatin to Induce Angptl4 and Jag1 Expression. S. Razavipour1, K. Jang2, H. Yoon2, M. Kim2, M. Shin2, D. Zhao2, D. Van Booven2, A. Bagheri1, J. Slingerland1 1Georgetown University, Washington, DC, 2University of Miami, Miami, FL
11:30 am MS14 The Identification and Characterization of FOLFOX Resistance in Colorectal Cancer Patient Derived Organoids. J. Morris University of Arizona, Tucson, AZ
11:35 am MS15 Multivesicular Body Fusion and Exosome Secretion Links Repair of Single Cell Injuries to Tissue-Wide Health. D. C. Bittel, M. Hogarth, A. Defour, J. Jaiswal Children's National Medical Center, Washington DC, DC
11:40 am MS16 Temporal and spatial topography of proliferation in cancer. S. Kabraji1, G. Gaglia2, D. Rammos2, Y. Dai2, A. Verma2, J. Bergholz1, W. Wang1, S. Wang3, C. Mills3, M. Chung3, S. Coy2, J.-R. Lin3, R. Jeselsohn1, O. Metzger1, E. P. Winer4, D. Dillon2, J. J. Zhao1, P. Sorger3, S. Santagata2 1Dana Farber Cancer Institute, Boston, MA, 2Brigham and Women's Hospital, Boston, MA, 3Harvard Medical School, Boston, MA, 4Yale Cancer Center, New Haven, CT
11:45 am MS17 Local fat content determines global and local stiffness in fatty livers. D. Li1,2, P. A. Janmey2,2,3,1, R. G. Wells1,2,2 1Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA, 2University of Pennsylvania, Philadelphia, PA, 3Institute for Medicine and Engineering, Philadelphia, PA
11:50 am MS18 Multiomics Analysis for Characterization of the Contribution of High Fructose Corn Syrup to Non-Alcoholic Fatty Liver Disease in Obesity. G. Papadopoulos1, A.-I. Legaki1, P. Vorkas2, G. Stamatakis3, I. Moustakas1, H. Gika4, M. Samiotaki3, A. Chatzigeorgiou1 1National and Kapodistrian University of Athens, Athens, Greece, 2Centre for Research and Technology, Thessaloniki, Greece, 3Biomedical Sciences Research Center “Alexander Fleming”, Athens, Greece, 4Aristotle University of Thessaloniki, Thessaloniki, Greece
11:55 am MS19 “Impact of the ER Stress Sensing Protein PERK and Autophagy On Exacerbated Strokes in Alzheimer’s Patients “. H. Gudlavalleti, R. Viswanthan, B. Stoveken, H. Deborah, D. Lozano, N. Derosa, s. Weintraub, k. Bieniek, J. Lechleiter University of Texas Health Science Center - San Antonio, San Antonio, TX
12:00 pm MS20 WIPI2B/ATG-18 Phosphorylation Regulates Neuronal Autophagosome Biogenesis in vivo. H. Tsong, A. Stavoe University of Texas Hlth Sci Ctr, Houston, TX
Track(s): Cellular Dynamics; and Physical Cell
Room 207A
11:15 am MS21 Regulation of Cell Dynamics by Rapid Transport of Integrins Through the Biosynthetic Pathway. M. Lerche1,2, M. Mathieu1,2, L. Malerød3,4, N. Pedersen3,4, H. Hamidi1,2, M. Chastney1,2, B. Bruininks5, S. Kaptan5, G. Jacquemet1,6,7, I. Vattulainen5, P. Roca-Cusachs8,9, A. Brech3,4, F. Perez10, G. Boncompain10, S. Miserey10, J. Ivaska1,2,11 1Turku Bioscience Centre, Turku, Finland, 2University of Turku, Turku, Finland, 3Institute for Cancer Research, Oslo University Hospital, Oslo, Norway, 4Institute of Clinical Medicine, University of Oslo, Oslo, Norway, 5University of Helsinki, Helsinki, Finland, 6Åbo Akademi University, Turku, Finland, 7Turku Bioimaging, University of Turku and Åbo Akademi University, Turku, Finland, 8Institute for Bioengineering of Catalonia (IBEC), the Barcelona Institute of Technology (BIST),, Barcelona, Spain, 9Universitat de Barcelona, Barcelona, Spain, 10Institut Curie, PSL Research University, Sorbonne Université, Centre National de la Recherche Scientifique, Paris, France, 11Foundation for the Finnish Cancer Institute, Helsinki, Finland
11:20 am MS22 Fibronectin Fibrillogenesis Is Mediated by Autocrine TGF-β1-dependent Secretion of Soluble Fibronectin. M. Sofroniou, 1st, C. Lemmon Virginia Commonwealth University, Richmond, VA
11:25 am MS23 Dissection of a potentially ancient septin in the emerging model black fungus Knufia petricola. G. E. Hamilton, A. S. Gladfelter University of North Carolina, Chapel Hill, NC
11:30 am MS24 Distinct Pathogenic Mutations in LRRK2 Disrupt Axonal Transport of Autophagosomes. D. Dou1, C. Boecker2, J. Goldsmith1, E. L. F. Holzbaur1 1University of Pennsylvania, Philadelphia, PA, 2University of Gottingen, Gottingen, Germany
11:35 am MS25 Regulation of aVb6 integrin to facilitate TGFb1 activation. M. Bachmann, J. Kessler, B. Wehrle-Haller Centre Medical Universitaire, Geneva, Switzerland
11:40 am MS26 MINFLUX facilitates the analysis of macromolecular complexes via light microscopy. C. A. Wurm, M. Velasco, K. Bahlmann Abberior Instruments America, Jupiter, FL
11:45 am MS27 A novel sub-precision detection method (MCS-DETECT) identifies shape complexity of mitochondria-ER contacts (MERCs) in 3D STED super-resolution microscopy. B. Cardoen1, K. Vandevoorde2, G. Gao2, P. Alan2, W. Liu2, A. Vogl2, G. Hamarneh1, I. R. Nabi2,2 1Simon Fraser University, Burnaby, BC, Canada, 2University Of British Columbia, Vancouver, BC, Canada
11:50 am MS28 Label-free morphological and biochemical characterization of pancreatic secretory cells. V. Loconte1, A. Deshmukh2, J. Cuala2, B. Vanslembrouck1, K. Chang2, K. White2 1University of California, San Francisco, San Francisco, CA, 2University of Southern California, Los Angeles, CA
11:55 am MS29 Single Molecule Spatial Transcriptomics Demonstrates Spatial Heterogeneity of miRNA and Classifies beta Cells in Type 1 Diabetes. G. Chang, C. Seitz, F. Syed, C. Evans-Molina, J. Liu Indiana University-Purdue University Indianapolis, Indianapolis, IN
12:00 pm MS30 Novel Microscopy Tools Reveal Dynamic Sub-cellular Distributions of Circadian Clock Components in Filamentous Fungus. Z. Wang, B. M. Bartholomai, J. J. Loros, J. C. Dunlap Geisel School of Medicine at Dartmouth, Hanover, NH
Track(s): Cellular Dynamics; and Physical Cell
Room 207B
11:15 am MS31 Using cryo-EM to visualize snapshots of dynein’s activation pathway. A. A. Kendrick, A. E. Leschziner, S. L. Reck-Peterson University of California, San Diego, San Diego, CA
11:20 am MS32 Investigating the role of γ-TuRC minus-end capping in microtubule regulation in vitro and in cell division. A. Y. Berman, M. Wieczorek, T. M. Kapoor The Rockefeller University, New York, NY
11:25 am MS33 Ninein expression and involvement in phagocytosis in macrophages. S. Omer, N. Gandhi, R. Harrison University of Toronto, Scarborough, ON, Canada
11:30 am MS34 The ubiquitous Microtubule Associated Protein 4 (MAP4) controls organelle distribution by regulating the activity of the kinesin motor. I. Nabti1, B. J. N. Reddy2, R. Rezgui1, W. Wang2, S. P. Gross2, G. T. Shubeita1 1New York University Abu Dhabi, Abu Dhabi, United Arab Emirates, 2University of California Irvine, Irvine, CA
11:35 am MS35 A dynein-Lis1 structure captures an intermediate state in dynein’s activation pathway. E. P. Karasmanis1, J. M. Reimer1, A. A. Kendrick1, J. A. Rodriguez1, J. B. Truong1, A. E. Leschziner1, S. L. Reck-Peterson1,2 1University of California San Diego, San Diego, CA, 2Howard Hughes Medical Institute, Chevy Chase, MD
11:40 am MS36 Dystrophin and Ensconsin Cooperate to Maintain Nuclear Spacing in Myofibers by Regulating Distinct Aspects of Microtubule Organization. J. R. Padilla, A. Burgess, G. Aleck, L. Ferreira, E. S. Folker Boston College, Chestnut Hill, MA
11:45 am MS37 Regulation of microtubule acetylation during the DNA damage response promotes efficient repair. S. S. Wallis, S. A. Godinho Barts Cancer Institute, London, United Kingdom
11:50 am MS38 Differential regulation of sliding microtubule bundles in metaphase and anaphase. S. Forth, E. Tai, A. Alfieri Rensselaer Polytechnic Institute, Troy, NY
11:55 am MS39 Augmin prevents merotelic attachments by promoting proper arrangement of kinetochore and bridging fibers. V. Štimac, I. Koprivec, I. Tolić Rudjer Boskovic Institute, Zagreb, Croatia
12:00 pm MS40 E3 ubiquitin ligase, FBXW7 targets centriole duplication protein, STIL for degradation. U. Anand, B. Badarudeen, A. Muliyil, M. Ebrahim, A. Asokan, R. Khurup, T. K. Manna IISER TVM, Thiruvanathapuram, India
Track(s): Cellular Dynamics; and Communal Cell
Room 146C
11:15 am MS41 PP2A-B55SUR-6 promotes nuclear envelope breakdown in C. elegans one-cell embryo. S. Kapoor, K. Adhikary, S. Kotak Indian institute of Science (IISc) Bangalore, Karnataka, India
11:20 am MS42 Architectural basis for cylindrical self-assembly governing Plk4-mediated centriole duplication in humans. J. Ahn, L. Zhang, H. Ravishankar, K. Kirsch, Y. Zeng, J.-E. Park, K. Lee National Institutes of Health, BETHESDA, MD
11:25 am MS43 Kinetochore centering and oscillations are regulated by overlap-length dependent forces. P. Risteski, J. Martinčić, M. Jagrić, I. M. Tolić Ruder Boskovic Institute, Zagreb, Croatia
11:30 am MS44 Dynamic transcription start site usage and RNA binding protein complexing regulate GJA1 mRNA translation to modulate gap junction formation. E. E. Shrontz1,2, M. J. Zeitz1, J. González Velázquez1, C. C. James1,3, J. W. Smyth1,2,4 1Fralin Biomedical Research Institute, Roanoke, VA, 2Virginia Tech Carilion School of Medicine, Ronaoke, VA, 3Virginia Tech, Blacksburg, VA, 4College of Science, Virginia Tech, Blacksburg, VA
11:35 am MS45 Cell cycle genes and oral regeneration in Stentor coeruleus. C. Yan, P. Sood, A. Lin, R. McGillivary, W. Marshall University of California, San Francisco, San Francisco, CA
11:40 am MS46 MPS1-mediated potentiation of Aurora A activity causes spindle orientation defects in the Drosophila follicular epithelium. N. Leça1,2, M. Osswald1, C. E. Sunkel1,2, E. Morais-de-Sá1, C. Conde1 1i3S - Institute for Research and Innovation in Health, Porto, Portugal, 2ICBAS - Institute of Biomedical Sciences Abel Salazar, Universidade do Porto, Porto, Portugal
11:45 am MS47 The dynamin-like protein Dnm1 mediates scission of the outer nuclear membrane in nuclear autophagy. P. J. Mannino, S. Chandra, D. J. Thaller, N. R. Ader, T. J. Melia, C. P. Lusk Yale University, New Haven, CT
11:50 am MS48 Mis-segregated DNA blocks an abscission promoting phosphatase pathway to halt cytokinesis. T. C. Panagiotou, A. Wilde,1 University of Toronto, Toronto, ON, Canada
11:55 am MS49 Investigating Centrosome Inactivation during Cellular Differentiation. R. K. Ng, J. Magescas, J. L. Feldman Stanford University, Stanford, CA
12:00 pm MS50 The Kash5 protein involved in meiotic chromosomal movements is a novel dynein activating adaptor. R. Agrawal1, J. P. Gillies1, J. L. Zang1, J. Zhang2, S. R. Garott1, H. Shibuya*2, J. Nandakumar*1, M. DeSantis*1 1University of Michigan, Ann Arbor, MI, 2University of Gothenburg, Gothenburg, Sweden
Track(s): Cellular Dynamics
Room 201
11:15 am MS51 A dynamic mechanically stabilized membrane reservoir mediates fast endocytosis. D. An1, R. Su1, P. J. Wen2, C. Chan2, L.-G. Wu2, B. O'Shaughnessy1 1Columbia University, New York, NY, 2National Institute of Neurological Disorders and Stroke, Bethesda, MD
11:20 am MS52 Golgi-derived vesicles facilitate the fission of lysosomal tubules by modulating lysosomal PI(3)P levels. M. Boutry1, L. DiGiovanni1, N. Demers1, A. Fountain2, S. Mamand2, R. Botelho2, P. K. Kim1 1The hospital for Sick Children, Toronto, ON, Canada, 2Toronto Metropolitan University, Toronto, ON, Canada
11:25 am MS53 Plasma Membrane Damage-Induced Senescent Cells Accelerate Wound Healing via Extracellular Vesicles and Soluble Molecules. H. Barbee, Y. Moriyama, K. Kono OIST, Onna, Japan
11:30 am MS54 Relationship between mitochondrial transport and neuronal architecture in vivo. E. Donovan, N. Bell, E. Sarkar, E. Barnhart Columbia University, New York, NY
11:35 am MS55 BAR Domain Proteins and Actomyosin Regulate Fusion Pore Dynamics of Large Secretory Vesicles. T. Biton, N. Scher, E. Schejter, B.-Z. Shilo, O. Avinoam Weizmann Inst Science, Rehovot, Israel
11:40 am MS56 The characterization and functional analysis of IRSp53-mediated secretion of the extracellular vesicles from tumor cells. H. T. Hu, T. Nishimura, S. Suetsugu Nara Institute of Science and Technology, Ikoma, Nara, Japan
11:45 am MS57 Lysosomes contain an expansion compartment that mediates zinc transporter delivery to promote zinc homeostasis inC.elegans. A. Mendoza1, N. Dietrich2, C. Tan3, D. Herrera1, J. Kasiah1, Z. Payne1, D. Schneider1, K. Kornfeld1 1Washington University, St. Louis, MO, 2NIEHS, Epigenetics and Stem Cell Biology Laboratory, Durham, NC, 3California Institute of Technology, Pasadena, CA
11:50 am MS58 CED-3 caspase and opposing kinesins direct mitochondrial confinement to govern Compartmentalized Cell Elimination. R. Sharmin1, S. P. Carmona2, G. Clark1, K. Juanez1, A. Elkhalil1, M. W. Pellegrino1, S. Shaham2, P. Ghose1 1University of Texas at Arlington, Arlington, TX, 2The Rockefeller University, New York, NY
11:55 am MS59 Loss of intestinal peroxisomes increases susceptibility to Citrobacter rodentium infection in mice. E. Miraglia1,2,1, M. Munawar1,2, S. Leung1, R. Bandsma1,2, P. Kim1,2 1Hospital for Sick Children, Toronto, ON, Canada, 2University of Toronto, Toronto, ON, Canada
12:00 pm MS60 Elucidation of Genes Regulating Peroxisome Homeostasis in Human Cells. J. T. Vu, K. Tavasoli, M. Morrissey, C. D. Richardson, B. M. Gardner University of California, Santa Barbara, Santa Barbara, CA
Track(s): Communal Cell; and Physical Cell
Room 147A
11:15 am MS61 Dysregulated trophoblast invasion and differentiation in Preeclampsia results from a reactive oxygen species-mediated endoplasmic reticulum stress pathway. I. Mukherjee1,2, S. Singh1, J. B. Sharma3, S. Biswas4, R. Dhar1, S. Karmakar1 1Department of Biochemistry, All India Institute of Medical Sciences, New Delhi, INDIA, 2Amity Institute of Biotechnology, Amity University, Noida, Uttar Pradesh, INDIA, 3Department of Obstetrics & Gynecology, All India Institute of Medical Sciences, New Delhi, INDIA, 4Amity Institute of Molecular Medicine & Stem Cell Research, Amity University, Noida, Uttar Pradesh, INDIA.
11:20 am MS62 Revealing the cellular mechanisms of tracheal-esophageal separation through live imaging. R. Yan, C. Tabin Harvard Medical School, Boston, MA
11:25 am MS63 Alpha-lipoic acid Attenuates Heat stress-induced Oxidative Stress in Porcine Embryos. S.-H. Lee, X.-S. Cui Chungbuk National University, Cheongju, Korea, Republic of
11:30 am MS64 Optogenetic rescue of a patterning mutant. H. E. Johnson1, N. J. V. Djabrayan2, S. Y. Shvartsman3, J. E. Toettcher3 1The University of Hong Kong, Hong Kong, Hong Kong, 2Clark University, Boston, MA, 3Princeton University, Princeton, NJ
11:35 am MS65 Whole-embryo analysis of local cell arrangement links differential cadherin expression to specific tissue architecture. M. Brambach1,2, D. Gilmour1 1University of Zurich, Zurich, Switzerland, 2Life Science Zurich Graduate School, Zurich, Switzerland
11:40 am MS66 Evaluating airway ALI model fabrication methods and comparing differentiation potential of primary and hTERT-immortalized epithelial cells. K. Tyo, F. Tian ATCC, Gaithersburg, MD
11:45 am MS67 Exploring the dynamic mechanical interactions of extruding cells and their neighbors:
Do apoptotic cells leave? or are they pushed?. S. Anjum1, Y. Atieh2, G. T. Eisenhoffer2, L. A. Davidson1 1University of Pittsburgh, Pittsburgh, PA, 2University of Texas MD Anderson Cancer Center, Houston, TX
11:50 am MS68 Evaluating neuroprotective effects of sulforaphane in a VPA-induced autism model. R. Bessetti1, V. Koonce1, A. Papariello1, C. Camacho1, K. McCoy2, K. Litwa1 1East Carolina University/Anatomy and Cell Biology, Greenville, NC, 2Florida Atlantic University, Boca Raton, FL
11:55 am MS69 Actomyosin Pulsing Protects Embryo Tissue Folding from Unavoidable Fluctuations due to Inherent Stochasticity in Cellular Systems. H. Zhu, B. O'Shaughnessy Columbia University, New York City, NY
12:00 pm MS70 How Cells Program Mineral Shape in Sea Cucumbers - Part 1. P. Vyas, C. Brannon, L. Formery, C. Lowe, M. Prakash Stanford University, Stanford, CA
Monday, December 5, 11:15 am to 12:05 pm
Track(s): Cells in Distress and Disease; and Physical Cell
Room 147A
11:15 am MS71 ZNF217 and NRG1/ErbB3 Signaling Promote Endocrine Therapy Resistance via the Ligand-Independent Activation of ER Alpha. P. Tennakoon1, Z. Song1, J. Wu1, K. Carthy1, M. Miramontes1, R. Goyal2, C. Suarez1, J. Li1, L. E. Littlepage1 1University of Notre Dame, Notre Dame, IN, 2University of Texas, Austin, TX
11:20 am MS72 Fibrosis to Fitness: Examining the Role of Tumor Stiffness in Pancreatic Ductal Adenocarcinoma. J. M. Rosas, J. Campanale, J. L. Harwood, A. A. Pitenis, D. J. Montell University of California, Santa Barbara, Santa Barbara, CA
11:25 am MS73 Substrate Stiffness Regulates Focal Adhesion Kinase Alternative Splicing in Breast Cancer. M. Millet, E. Pic, C. Luthold, G. Bourriquen, G. Khelifi, B. Trudel, S. Hussein, F. Bordeleau Université Laval, Québec, QC, Canada
11:30 am MS74 ARID1A loss leads to enhancement of genomic instability by epigenetic dysregulation in aggressive Osteosarcoma. K. Fatema1, Y. Wang2, S. Plyler1, A. Pavek1, K. Jones2, J. Barrott1 1Idaho State University, Pocatello, ID, 2University of Utah, SLC, UT
11:35 am MS75 Examining the role of EGFR family signaling in promoting prostate cancer proliferation in an androgen receptor-low environment. S. Wilkinson1, A. Ku1, I. King1, A. Baj1, D. Low1, H. Ye2, F. Karzai1, S. A. Harmon1, N. T. Terrigino1, J. R. Bright1, S. Y. Trostel1, R. T. Lis1, B. Turkbey1, W. L. Dahut1, A. G. Sowalsky1 1National Cancer Institute, Bethesda, MD, 2UCLA, Los Angeles, CA
11:40 am MS76 Exploiting age-dependent biomechanical features of single cells for disease detection and prevention. S. Hinz1, M. Miyano1, A. Manousopoulou1, R. W. Sayaman1,2, K. Y. Aguilera3, M. E. Todhunter1, J. C. Lopez1, L. D. Wang1, L. L. Sohn4, M. A. LaBarge1 1City of Hope, Duarte, CA, 2University of California, San Francisco, CA, 3David Geffen School of Medicine at UCLA, Los Angeles, CA, 4University of California at Berkeley, Berkeley, CA
11:45 am MS77 Co-treatment of gallic acid and cisplatin suppresses LPS-induced cancer properties in MDA-MB 231 breast cancer cells. A. R. Aikins, P. Pasgo, J. T. Quarshie, S. M. Baffoe, P. A. Birikorang, Y. Bediako, W. S. K. Gbewonyo University of Ghana, Accra, Ghana
11:50 am MS78 Emerin dysregulation compromises nuclear integrity during metastatic transformation. E. Hansen, J. M. Holaska Cooper Medical School At Rowan, Camden, NJ
11:55 am MS79 Sphingosine Kinase 1 Promotes Lung Cancer Cell Growth. A. B. Nguyen1, V. Balaji Ragunathrao2, D. Mehta2 1Villanova University, Villanova, PA, 2The University of Illinois College of Medicine, Chicago, IL
12:00 pm MS80 Identifying Golgi glycosyltransferase B4GALT1 as a p53-responsive gene in lung cancer. S. Bui, D. Stark, A. Houmani, Y. Wang University of Michigan, Ann Arbor, MI
Track(s): Signaling and Metabolism; and Cellular Dynamics
Room 207B
11:15 am MS81 Spatiotemporal crosstalk of Rap1A-RhoA GTPases regulates breast cancer cell motility. C. Sanchez, A. Muesch, L. Hodgson Albert Einstein College of Medicine, Bronx, NY.
11:20 am MS82 The conserved RNA binding protein Orb2 upregulates rare codon biased transcripts during differentiation of Drosophila neurons. R. K. Stewart, S. Allen, D. Fox Duke University, Durham, NC
11:25 am MS83 Irisin acts through its integrin receptor in a novel two-step process. M. A1,2, T. E. Wales3, H. Zhou2, M. J. Mittenbühler1,2, C. R. Kim1,2, S.-V. Draga-Coletă4,5, C. Gorgulla1,6,2, K. A. Blackmore1,2, Z.-F. Wang1,2, M. P. Jedrychowski1,2, H.-S. Seo1,2, K. Song1,2, A. Z. Xu1,2, L. Sebastian1,2, D. Bogoslavski1,2, S. P. Gygi2, H. Arthanari1,2, S. Dhe-Paganon1,2, P. R. Griffin7,8, J. R. Engen3, B. M. Spiegelman1,2 1Dana-Farber Cancer Institute, Boston, MA, 2Harvard Medical School, Boston, MA, 3Northeastern University, Boston, MA, 4Virtual Discovery, Inc, Chestnut Hill, MA, 5Non-Governmental Research Organization Biologic, Bucharest, Romania, 6Harvard University, Cambridge, MA, 7UF Scripps Biomedical Research, Jupiter, FL, 8Scripps Research, Jupiter, FL
11:30 am MS84 Analyzing Site-specific and Direct Interactions of AAA Proteins with Cofactors and Substrates in Living Cells. L. E. Vostal,1, W. Zhang, B. T. Chait, T. M. Kapoor The Rockefeller University, New York, NY
11:35 am MS85 ARHGAP17 regulates the spatiotemporal activity of Cdc42 at invadopodia. G. Kreider-Letterman1, A. Castillo1, E. Mahlandt2, J. Goedhart2, A. Rabino1, S. Goicoechea1, R. Garcia-Mata1 1University of Toledo, Toledo, OH, 2University of Amsterdam, Amsterdam, Netherlands
11:40 am MS86 Compartment-Specific Activation of Proton-Sensor GPR65 is Uncoupled from Receptor Trafficking. L. M. Morales-Rodriguez, M. Puthenveedu University of Michigan, Ann Arbor, MI
11:45 am MS87 Translational Regulation by Desmosomes. D. Hlavaty1, K. Badu-Nkansah1, G. Bjerke2, R. Yi3, T. Lechler1 1Duke University, Durham, NC, 2University of Colorado Boulder, Boulder, CO, 3Northwestern University, Evanston, IL
11:50 am MS88 A hard path to follow: The LKB1-AMPK axis in mechanosensing and durotaxis. A. K. Howe, J. B. Patterson University of Vermont, Burlington, VT
11:55 am MS89 The Disulfide Bonds in the C-terminal Domains of the Insulin Receptor Regulate Insulin-mediated Receptor Endocytosis. S. H. Park, E. Choi Columbia University, New York, NY
12:00 pm MS90 Mechanical Memory Acquisition of Oral Squamous Cell Carcinoma (OSCC) and Biological Pathways Involved in the Process. S. Moon1, P. S. Campos2, B. F. Matte2, J. K. Placone3, V. G. Zanella2, M. D. Martins2, M. L. Lamers2, A. J. Engler1 1University of California San Diego, La Jolla, CA, 2Federal University of Rio Grande do Sul, Porto Alegre, Brazil, 3West Chester University, West Chester, PA
Track(s): Cellular Dynamics
Room 202B
11:15 am MS91 Human Artificial Chromosomes as a Tool to Discover New Human CIN Genes. M. Liskovykh1, E. Chiang2, K. Cheng2, H. Masumoto3, W. Earnshaw4, N. Kouprina1, V. Larionov1 1National Cancer Institute, Bethesda, MD, 2NCATS, Rockville, MD, 3Kazusa DNA Research Institute, Chiba, Japan, 4Wellcome Centre for Cell Biology, Edinburgh, United Kingdom
11:20 am MS92 The binding and elongation properties of tropomyosin Tm1A to actin filaments are highly context dependent. N. A. Petek-Seoane, J. Rodriguez, D. Mullins University of California, San Francisco, san francisco, CA
11:25 am MS93 Polyglutamylation of microtubules drives motor axon remodeling. A. Gavoci1, M. Wang1, N. Marahori1, C. Janke2, M. Magiera3, T. Misgeld1, M. S. Brill1 1Technical Universtiy Munich, institute of Neuronal Cell Biology, Munich, Germany, 2Institut Curie, PSL Research University, CNRS UMR3348, F-91401 Orsay, France; Université Paris-Saclay, CNRS UMR3348, F-91401 Orsay, France, Orsay, France, 3Institut Curie, PSL Research University, CNRS UMR3348, F-91401 Orsay, France; Université Paris-Saclay, CNRS UMR3348, Orsay, France
11:30 am MS94 Tubulin arginylation regulates microtubule dynamics via MAP1S. B. MacTaggart, A. Kashina University of Pennsylvania, Philadelphia, PA
11:35 am MS95 A novel probe illuminates the chemical logic of microtubule detyrosination. Y. Yue, T. Hotta, K. J. Verhey, R. Ohi University of Michigan Medical School, Ann Arbor, MI
11:40 am MS96 Clustering of the scaffolding protein CENP-T activates recruitment of Ndc80 complexes to assemble a functionally active outer kinetochore. E. V. Tarasovetc1, G. B. Sissoko2,3, A. Maiorov1, I. M. Cheeseman2,3, E. L. Grishchuk1 1University of Pennsylvania, Philadelphia, PA, 2Massachusetts Institute of Technology, Cambridge, MA, 3Whitehead Institute for Biomedical Research, Cambridge, MA
11:45 am MS97 Understanding chromosome segregation by drug-mediated tension responses. V. Todi, M. Gupta Iowa State University, Ames, IA
11:50 am MS98 Acetylation regulates multiple functions of the TOG protein Stu2, an XMAP215/CKAP5 homologue. M. A. Greenlee, B. Witt, J. Sabo, S. C. Morris, R. K. Miller Oklahoma State University, Stillwater, OK
11:55 am MS99 Inhibitor-2 promotes Protein Phosphatase 1 activity and mitotic progression in the C. elegans embryo. N. Varshney, K. Oegema, A. Desai University of California San Diego, San Diego, CA
12:00 pm MS100 Microtubule polymerase XMAP215/Mini spindles and cytoplasmic dynein are required for the oocyte determination in Drosophila. W. Lu, M. Lakonishok, V. I. Gelfand Northwestern University Feinberg Sch Med, Chicago, IL
Track(s): Cells in Distress and Disease; and Specialized Cell and Evolution
Room 146C
11:15 am MS101 The fungal pathogen Cryptococcus actively manipulates maturation of its phagosome in an immune signal-dependent way. P. Stuckey1, E. Santiago-Burgos2, F. Santiago-Tirado1 1University of Notre Dame, Notre Dame, IN, 2Imanis Life Sciences, LLC, Rochester, MN
11:20 am MS102 Phagocytosis-dependent activation of macrophage Nrf2 curbs intracellular ROS levels to strengthen the inflammatory response. G. Clemente, H. Weavers University of Bristol, Bristol, United Kingdom
11:25 am MS103 The Role of Bacterial Filamentation in the Protection Against Human Cathelicidin Antimicrobial Peptide LL-37. N. Porco, M. Tomlinson, R. J. Botelho, J. B. McPhee Toronto Metropolitan University, Toronto, ON, Canada
11:30 am MS104 Mechanosensitive recruitment of Vinculin maintains junction integrity and barrier function at tricellular junctions in vertebrate epithelia. L. Van Den Goor, J. Iseler, A. L. Miller University of Michigan, University of Michigan, MI
11:35 am MS105 An ancestral mycobacterial effector promotes dissemination of infection. M. I. Sweeney, J. W. Saelens, G. Viswanathan, A. M. Xet-Mull, J. Coers, J. E. Stout,1, D. M. Tobin Duke University, Durham, NC
11:40 am MS106 Adaptive immune receptor repertoire profiling for biomarker discovery. A. Chenchik, M. Makhanov, T. Liu, D. Hu, K. Ghias, P. Diehl, L. Kobzik Cellecta, Inc., MOUNTAIN VIEW, CA
11:45 am MS107 Damaged mitochondria recruit the effector NEMO to activate NF-κB. O. Harding, E. L. F. Holzbaur University of Pennsylvania, Philadelphia, PA
11:50 am MS108 Biophysical Characterization of Cytotoxic T cell Heterogeneity in Cancer Progression. Y. Zhang1, J. Yu1, T. Dinter1,1, R. Wu1,1, S. Duquette1,1, T. Miettinen1, A. Miller1,2, S. Spranger1,1,3, S. R. Manalis1,1,1 1Massachusetts Institute of Technology, Cambridge, MA, 2Department of Health Sciences and Technology, Harvard University - Massachusetts Institute of Technology, Cambridge, MA, 3Ragon Institute of MGH, MIT and Harvard, Cambridge, MA
11:55 am MS109 Nucleus uptake of water soluble C60 derivatives: a molecular platform for targeting cargo to diseased cells. R. Biswas, S. Yang, Z. Shi, J. Zhang Rutgers University, New Brunswick, NJ
12:00 pm MS110 Mycobacterium tuberculosis utilizes serine/threonine kinase PknF to evade NLRP3 inflammasome dependent interleukin-1beta secretion. S. Rastogi, V. Briken University of Maryland, College Park, College Park, MD
Track(s): Cellular Dynamics; and Signaling and Metabolism
Room 207A
11:15 am MS111 Topological Regulation of a Transmembrane Protein by Luminal-to-Cytosolic Retrotranslocation of Glycosylated Sequence. J. Wang*, S. Han*, J. Ye UT Southwestern Medical Center, Dallas, TX
11:20 am MS112 A type I myosin critical for clathrin-mediated endocytosis is a force-generating motor. R. T. A. Pedersen1,2, A. Snoberger3, S. Pyrpassopoulos3, D. Safer3, D. G. Drubin1, E. M. Ostap3 1University of California, Berkeley, Berkeley, CA, 2Carnegie Institution of Washington, Baltimore, MD, 3University of Pennsylvania Perelman School of Medicine, Philadelphia, PA
11:25 am MS113 Select autosomal dominant DFNA11 deafness mutations activate Myosin 7a. P. Acharya, G. Thapa, T. Nguyen, S. W. Crawley University of Toledo, Toledo, OH
11:30 am MS114 Molecular mechanisms of SURF4-mediated protein secretion. J. Maldutyte, N. Gomez-Navarro, S. Peak-Chew, E. A. Miller Cell Biology, MRC Laboratory of Molecular Biology, Cambridge, UNITED KINGDOM.
11:35 am MS115 Acute COG inactivation unveiled its immediate impact on Golgi & illuminated the nature of intra-Golgi recycling vesicles. F. Sumya, I. Pokrovskaya, Z. D'Souza, V. Lupashin UAMS, Little Rock, AR
11:40 am MS116 Role of Sfi1 proteins in Stentor coeruleus membranellar band regeneration. C. Yan, P. Sood, R. McGillivary, A. Lin, W. Marshall University of California, San Francisco, San Francisco, CA
11:45 am MS117 The sets of kinesins and dyneins transporting endocytic cargoes governs their response to tau. A. G. Hendricks1, D. Beaudet1, L. Balabanian1, C. L. Berger2 1McGill University, Montreal, QC, Canada, 2University of Vermont, Burlington, VT
11:50 am MS118 Biogenesis of neuronal exosomes via ESCRT machinery. E. C. Dresselhaus, M. Rozencwaig, R. Soslowsky, M. F. Pescosolido, B. Ermanoska, A. A. Rodal Brandeis University, Waltham, MA
11:55 am MS119 Impact of MYO3A Motor Properties on Actin Protrusion Length and Dynamics. J. A. Cirilo, Jr.1, X. Liao2, B. J. Perrin2, C. M. Yengo1 1Penn State Univ/Milton S Hershey Medical Ctr, Hershey, PA, 2Indiana University-Purdue University Indianapolis, Indianapolis, IN
12:00 pm MS120 Two O-GlcNAcylation Sites in the Disordered Region of Sec24D are Critical for Collagen Traffickingin vivo. T. Hirata1, D. Choudhary2, B. Bisnett1, N. Linhart1, E. Knapik2, M. Boyce1 1Duke University, Durham, NC, 2Vanderbilt University Medical Center, Nashville, TN
Track(s): Cellular Dynamics; and Specialized Cell and Evolution
Room 201
11:15 am MS121 Vimentin intermediate filaments scaffold Endoplasmic Reticulum (ER) tubule matrices and pattern global ER mass distribution via Nesprin3/Plectin. A. S. Moore, J. Lippincott-Schwartz Howard Hughes Medical Institute, Ashburn, VA
11:20 am MS122 Multivesicular body (MVB) maturation involves immobilization on a subset of microtubules by septin GTPases. B. P. Robinson, N. R. Bass, E. T. Spiliotis Drexel University, Philadelphia, PA
11:25 am MS123 Establishing a role for ATG2-mediated Lipid Transport at the Lipid Droplet. J. L. Korfhage, N. Wan, K. M. Reinisch, T. J. Melia Yale University, New Haven, CT
11:30 am MS124 In situ architecture of the lipid transport protein VPS13C at membrane contacts. S. Cai1,2,1,3, Y. Wu1,2,1,3, A. Guillén-Samander1,2,1,3, W. Hancock-Cerutti1,2,1,3, J. Liu1, P. DeCamilli1,2,1,3 1Yale University School of Medicine, New Haven, CT, 2Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, CT, 3Aligning Science Across Parkinson’s (ASAP) Collaborative Research Network, Chevy Chase, MD
11:35 am MS125 Transmembrane mucins regulate the morphology of the endoplasmic reticulum through negative curvature generation. M. C. Goudge, J. C. H. Kuo, M. J. Paszek Cornell University, Ithaca, NY
11:40 am MS126 TorsinA is essential for the timing and localization of neuronal nuclear pore biogenesis. S. Kim1, S. Phan2, D. Boassa2, T. R. Shaw3, S. S. Pappas4, M. H. Ellisman2, S. L. Veatch3, S. J. Barmada1, W. T. Dauer4 1University of Michigan Medical School, Ann Arbor, MI, 2University of California San Diego, San Diego, CA, 3University of Michigan, Ann Arbor, MI, 4UT Southwestern, Dallas, TX
11:45 am MS127 High Temporal Resolution Mapping of the Phosphorylation Footsteps of Ciliogenesis. R. E. Turn1, M. Aziz-Zanjani1, T. Kanie2, J. Demeter1, P. K. Jackson1 1Stanford University, Stanford, CA, 2University of Oklahoma Health Sciences Center, Oklahoma City, OK
11:50 am MS128 Enhanced Mitochondrial Biogenesis Promotes Cell Survival in Human Stem Cell Derived Retinal Ganglion Cells of the Central Nervous System. M. Surma, K. Anbarasu, K.-C. Huang, J. S. Meyer, A. Das Indiana University, Indianapolis, IN
11:55 am MS129 Scs2 modulates mitochondria-ER contact sites through Num1. J. C. Casler, H. L. Anderson, L. L. Lackner Northwestern University, Evanston, IL
12:00 pm MS130 The NTPase activity of the Double FYVE Domain Containing Protein 1 (DFCP1) regulates lipid droplet metabolism. V. A. Ismail, T. Naismith, D. J. Kast Washington University in St. Louis, Saint Louis, MO
Track(s): Communal Cell; and Cells in Distress and Disease
Room 145B
11:15 am MS131 Dysregulated epidermal keratinocyte dynamics contribute to chronic damage signaling and impaired healing of thermal burn injury. A. Horn, A. Fister, J. Squirrell, A. Huttenlocher University of Wisconsin, Madison, WI
11:20 am MS132 The Epithelial to Mesenchymal Transition (EMT) in early differentiation: integrating and interrogating cell organization, cell behavior, and cell identity. A. Borensztejn1, . Allen Institute for Cell Science1, J. A. Theriot2, C. Hookway1, S. M. Rafelski1 1Allen Institute for Cell Science, Seattle, WA, 2University of Washington, Seattle, WA
11:25 am MS133 Prdm1 Drives a Sensory Hair Cell Fate Switch in Zebrafish. J. E. Sandler, N. T. T. Tran, S. Chen, T. Piotrowski Stowers Institute for Medical Research, Kansas City, MO
11:30 am MS134 Analysis of the individual and collective behaviors of signaling filopodia during Notch-mediated patterning. S. Kundu, T. Smith, G. L. Hunter Clarkson University, Potsdam, NY
11:35 am MS135 Neuronal activity regulates matrin-3 in a calcium and calmodulin dependent manner. J. Wu, A. Malik, C. Gillies, Q. Doctrove, S. Barmada University of Michigan, Ann Arbor, MI
11:40 am MS136 Smart glues: coordinating robust pattern formation and morphogenesis with an adhesion code. M. Ryan, C.-H. Chiu, T. Tsai Washington University in St. Louis, St. Louis, MO
11:45 am MS137 Podoplanin (PDPN) Enables Transformed Cells to Migrate and Escape Growth Control Mediated by Cadherin Junctions with Adjacent Nontransformed Cells. E. P. Retzbach1, S. A. Sheehan1, H. Krishnan2, Y. Shen1, J. P. Trama3, H. Zheng4, G. S. Goldberg1 1Rowan University, Stratford, NJ, 2Stony Brook University, Stony Brook, NY, 3Medical Diagnostic Laboratories, Hamilton, NJ, 4Rutgers University, Piscataway, NJ.
11:50 am MS138 Optogenetic manipulation of brain cell development in the early embryo. K. Fenelon, S. Abbasi, A. Chandrasekhar, P. H. Borad, T. Koromila Biology, University of Texas Arlington, Arlington, TX.
11:55 am MS139 An Essential Role of Polyhomeotic Homolog 1 (Phc1) in Early Neural Development. A. L. C. Ong1, M. Shirai2, N. Sasai1 1Nara Institute of Science and Technology, Ikoma, Nara, Japan, 2National Cerebral and Cardiovascular Center, Osaka, Japan
12:00 pm MS140 Novel Mechanosensitive Junction Interactor Gish is Required for Apical Constriction and Epithelial Folding. R. Koran, M. Weng University of Nevada Las Vegas, Las Vegas, NV.
Tuesday, December 6, 11:15 am to 12:05 pm
Track(s): Cellular Dynamics; and Communal Cell
Room 202B
11:15 am MS141 Cytotoxic T Lymphocyte Activation Signals Modulate Cytoskeletal Dynamics and Mechanical Forces. A. Pathni, A. Özçelikkale, I. Rey-Suarez, S. Davis, N. Rogers, L. Li, Z. Xiao, A. Upadhyaya University of Maryland, College Park, MD
11:20 am MS142 Evolutionary diversification of Arp2 separated somatic versus germline roles. K. A. Stromberg1, T. Spain1, S. A. Tomlin2,3, C. M. Schroeder1 1University of Texas Southwestern Medical Center, Dallas, TX, 2Fred Hutchinson Cancer Research Center, Seattle, WA, 3Howard Hughes Medical Institute, Seattle, WA
11:25 am MS143 Growth of a Drosophila embryo actin cap begins with apparent coupling of Arp2/3 network induction and exocytosis at plasma membrane infolds organized by centrosomes. R. Tam, N. Vergara Ruiz, T. Harris University of Toronto-47731885, Toronto, ON, Canada
11:30 am MS144 The Roles and Regulation of Capping Protein in Cortical F-actin Network Organization in the C. elegans Zygote. S. E. Yde, R. S. Kadzik, E. Munro,1,1, D. R. Kovar,1,1,1 University of Chicago, Chicago, IL
11:35 am MS145 CLASP2 facilitates dynamic actin filament organization along the microtubule lattice. N. Rodgers, E. Lawrence, N. Efimova, G. Gonzalez-Vasquez, T. Hickman, I. Kaverina, M. Zanic Vanderbilt University, Nashville, TN
11:40 am MS146 Adipose triglyceride lipase promotes prostaglandin-dependent actin remodeling by regulating substrate release from lipid droplets. M. Giedt1, J. Thomalla2, M. Johnson2, Z. Lai3, T. Tootle1, M. Welte2 1University of Iowa-Carver College Med, Iowa City, IA, 2University of Rochester, Rochester, NY, 3Harvard T.H. Chan School of Public Health, Boston, MA
11:45 am MS147 Smooth muscle myosin 2 filaments dynamically assemble and activate during induced contractility. M. Bennett1, S. Sala1, S. Demeulenaere2, H. Patel1, P. Oakes1, J. Beach1 1Loyola University, Chicago, IL, 2Loyola University, Wilmington, IL
11:50 am MS148 Wiskott-Aldrich Syndrome protein promotes antigen-specific selection of germinal center B-cells by modulating antigen presentation and mitochondrial function. Y. Li1, R. Dai1, J. van Haaren2, A. Bhanja2, X. Zhao1, W. Song2 1Children's Hospital of Chongqing Medical University, Chongqing, China, 2University of Maryland, College Park, MD
12:00 pm MS149 Combinatorial readout of the tubulin code by katanin. E. Szczesna1, E. A. Zehr1, S. W. Cummings1, A. Szyk1, K. K. Mahalingan1, Y. Li1, A. Roll-Mecak1,2 1National Institute of Neurological Disorders and Stroke, NIH, Bethesda, MD, 2National Heart, Lung and Blood Institute, NIH, Bethesda, MD
12:05 pm MS150 E-cadherin dependent molecular and biophysical mechanism of epithelial phagocytic clearance in the early vertebrate embryo. H. M. Häkkinen1, E. Hoijman2, M. Batet Palau1,3, L. Bianchi1,2, S. Wieser4, V. Ruprecht1 1Cell and Developmental Biology, Centre for Genomic Regulation (CRG), Barcelona, SPAIN, 2Universitat de Barcelona (UB), Barcelona, SPAIN, 3Universitat Pompeu Fabra, Barcelona, SPAIN, 4The Institute of Photonic Sciences (ICFO), Barcelona, SPAIN.
Track(s): Cells in Distress and Disease; and Cellular Dynamics
Room 147A
11:15 am MS151 Vascular endothelial profilin-1 Inhibition suppresses tumor progression in renal cancer. D. Gau1, A. Allen1, A. Daoud1, J. Kunkel1, A. N. Paranjape2, F. S. Villanueva2, P. Roy1 1University of Pittsburgh, Pittsburgh, PA, 2UPMC, Pittsburgh, PA
11:20 am MS152 Investigating the Role of Compressive Stress in Cellular Unjamming. G. Cai1, A. Nguyen2, Y. Bashirzadeh1, S.-S. Lin1, D. Bi2, A. Liu1 1University of Michigan, Ann Arbor, MI, 2Northeastern University, Boston, MA
11:25 am MS153 Profilin-1/CCL2 is a novel signaling axis of tumor cell-directed migration of immune cells. I. Eder, V. Yu, M. Joy, P. Lucas, P. Roy University of Pittsburgh, Pittsburgh, PA
11:30 am MS154 Identification of PAKAP as a Novel Regulator of Myoblast Fusion in Vertebrates. A. Hazak, S. Sinha, G. Zarfati, K. Huk, E. Ozer-Partuk, O. Avinoam Weizmann Institute Of Science, Rehovot, Israel
11:35 am MS155 Investigating the roles of Etv4 and Etv5 during the self-renewal and differentiation of nephron progenitor cells in kidney organogenesis. K. Day1,2, K. Thorner1, A. Hosawi1, B. Hammond1,2, M. Fraunfelter1, M. Kofron1, F. D. Costantini3, C. Cebrian1,2 1Cincinnati Children's Research Foundation, Cincinnati, OH, 2University of Cincinnati, Cincinnati, OH, 3Columbia University, New York, NY
11:40 am MS156 RAI14 Drives Pancreatic Tumorigenesis and Supports Proliferative Mesenchymal Tumor Cells. F. Runa1, L. Tomaneng1, Y. Adamian1, C. Nathan2, C. Gonzalez1, J. Hong1, K. Bhakta1, M. Boyce2, J. Kelber1 1California State University Northridge, Northridge, CA, 2Duke University, Durham, NC
11:45 am MS157 Cells in hybrid Epithelial-to-Mesenchymal Transition state can induce invasivity of surrounding epithelial cells. J. Geay1,2, S. Seetharaman3, L. Blanchoin4,1, M. Théry1,2,5 1CytoMorpho Lab, CEA/CNRS/Unité de Thérapie Cellulaire, Hôpital Saint Louis, Paris, France, 2Institut Pierre Gilles de Gennes, Paris, France, 3James Frank Institute, Department of Physics, University of Chicago, Chicago, IL, 4CytoMorpho Lab, Interdisciplinary Research Institute of Grenoble, CEA/CNRS/Université Grenoble Alpes, Grenoble, France, 5CytoMorpho Lab, Laboratoire de Physiologie Cellulaire et Végétale, Interdisciplinary Research Institute of Grenoble, CEA/CNRS/Université Grenoble Alpes,, Grenoble, France
11:50 am MS158 Depletion of Rnd3 in lung cancer decreases both invasion and migration in a ROCK1 independent manner. N. Garcia Garcia, D. Richey, C. Ashby, K. R. Ryan University of Arkansas Medical Sci, Little Rock, AR
11:55 am MS159 Investigating Intrinsic and Extrinsic Mechanisms of GBM Invasion. M. J. Barraza1, A. Banisadr1, F. B. Furnari1, A. J. Engler1,2 1University of California, San Diego, La Jolla, CA, 2Sanford Consortium for Regenerative Medicine, La Jolla, CA
Track(s): Cells in Distress and Disease; and Signaling and Metabolism
Room 146C
11:15 am MS160 SmartHeart - a novel 3D in vitro assay for improved assessment of cardiac drug efficacy and toxicity. M. Seguret1, P. Davidson2, S. Robben2, C. Jouve1, C. Cerveau2, J. Hulot3, M. Le Berre2, R. S. R. Ribeiro2 1INSERM, PARCC, Université de Paris, Paris, FRANCE, 24Dcell, Montreuil, FRANCE, 3INSERM, PARCC, Université de Paris and CIC1418 and DMU CARTE, Assistance Publique Hôpitaux de Paris (AP-HP), Hôpital Européen Georges-Pompidou, Paris, FRANCE.
11:20 am MS161 Modulating Arginine Metabolism in Breast Tumor Microenvironment via Sepiapterin - A Novel Immunotherapeutic Strategy for Breast Cancer. V. R. Fernando, V. Sharma, X. Zheng, S. Furuta University of Toledo, Toledo, OH
11:25 am MS162 Characterization of gamma-glutamyltransferase 6 protein in breast cancer. G. Peguero Kushner, C. Smith, Z. Li, B. Papp University of Florida, Gainesville, FL
11:30 am MS163 Targeting MAPK/Hippo Pathway Interactions in Metastatic Melanoma. N. K. Aboud, L. García-Gutiérrez, W. Kolch, D. Matallanas University College Dublin, Dublin, IRELAND.
11:35 am MS164 Tardigrade small heat shock proteins can limit desiccation-induced protein aggregation. J. D. Hibshman1, S. Carra2, B. Goldstein1 1The University of North Carolina at Chapel Hill, Chapel Hill, NC, 2University of Modena and Reggio Emilia, Modena, Italy
11:40 am MS165 Cryptosporidium Uses its Cspv1 Virus to Activate Host Type I IFN Signaling and Attenuate Epithelial Antiparasitic Defense. S. Deng1,2, W. He2, A. Gong1,2, M. Li2, Y. Wang2, Z. Xia2, X.-T. Zhang2, A. S. H. Pacheco3, A. Naqib1, M. Jenkins4, P. C. Swanson2, K. M. Drescher2, J. K. Strauss-Soukup5, M. A. Belshan2, X.-M. Chen1,2 1Rush University Medical Center, Chicago, IL, 2Creighton University School of Medicine, Omaha, NE, 3University of Nebraska Medical Center, Omaha, NE, 4the United States Department of Agriculture, Beltsville, MD, 5Creighton University College of Arts and Sciences, Omaha, NE
11:45 am MS166 A small internal deletion in Titin yields transient cardiogenic defects followed by ion channel-dependent cardiomyopathy and arrhythmia. X. Jiang1,2, O. T. Ly1,1, H. Chen1, Z. Zhang1, B. A. Ibarra1,2, M. A. Pavel1, A. Sridhar1,1, D. Tofovic1, A. Swick1,2, R. Marszalek1, F. Navales1,2, Y. Gao1, D. Darbar1,1, A. Saxena1,2 1University of Illinois at Chicago, Chicago, IL, 2University of Illinois Cancer Center, Chicago, IL
11:50 am MS167 Klhl41 plays a critical role in er homeostasis in skeletal muscle. T. Moretti, J. Essner, J. Kim Iowa State University, Ames, IA
11:55 am MS168 Defining the differential function, localization, and protein interaction partners of wild type and expanded PABPN1, an RNA binding protein associated with a late onset muscle disease. A. T. Mezzell, K. E. Vest University of Cincinnati, Cincinnati, OH
12:00 pm MS169 Dissecting the synergistic role of the unfolded protein response in wound infections. S. Lee, C. Celik, A. Tan, M. Veleba, K. A. Kline, G. Thibault Nanyang Technological University, Singapore, Singapore
Track(s): Cellular Dynamics; and Cells in Distress and Disease
Room 145B
11:15 am MS170 A common mechanism of Sec61 translocon inhibition by small molecules. S. Itskanov1, L. Wang*1, T. Junne2, R. Sheriff1, L. Xiao3, N. Blanchard4, W. Q. Shi5, C. Forsyth3, D. Hoepfner6, M. Spiess2, E. Park1,1 1University of California, Berkeley, Berkeley, CA, 2University of Basel, Basel, Switzerland, 3The Ohio State University, Columbus, OH, 4Université de Haute-Alsace, Université de Strasbourg, Mulhouse, France, 5Ball State University, Muncie, IN, 6Novartis Pharma AG, Basel, Switzerland
11:20 am MS171 Measuring conformational changes in clathrin light chain at single sites of endocytosis with FRET-CLEM. K. Obashi, K. A. Sochacki, M.-P. Strub, J. W. Taraska National Heart, Lung, and Blood Institute, NIH, Bethesda, MD
11:25 am MS172 Liquid-liquid phase separation in the TGN mediates delivery of proinsulin to secretory storage granules. A. PARCHURE1, M. Tian1, D. Stalder2, S. K. Mahata3, S. B. Stephens4, D. Gershlick2, J. V. Blume1 1Yale University, New Haven, CT, 2University of Cambridge, Cambridge, United Kingdom, 3University of California San Diego, La Jolla, CA, 4University of Iowa, Iowa City, IA
11:30 am MS173 Regulated Secretion Mediates Yeast Cell Fusion. U. Machi1, E. Mazur1, A. Sundlof1, J. Smith2, M. Rose1 1Georgetown University, Washington, DC, 2Stetson University, DeLand, FL
11:35 am MS174 Molecular mechanism of TIM23-mediated mitochondrial protein import revealed by cryo-EM structure and crosslinking analysis. S. I. Sim, Y. Chen, E. Park University of California, Berkeley, Berkeley, CA
11:40 am MS175 Insulin Signaling Attenuates GLUT4 Endocytosis in Muscle Cells via GSK3α-Dyn2-Bin1 Interplay. J. Laiman, Y. Liu Institute of Molecular Medicine, College of Medicine, National Taiwan University, Taipei, TAIWAN.
11:45 am MS176 Mitochondrial-derived compartments are stress-induced multilamellar whorls that sequester mitochondrial outer membrane cargo. Z. N. Wilson1, A. M. English1, M. West2, G. Odorizzi2, A. L. Hughes1 1University of Utah, Salt Lake City, UT, 2University of Colorado Boulder, Boulder, CO
11:50 am MS177 Caveolin-1 organization and function drive functional plasticity in Ewing Sarcoma. D. Segal1, D. Rajendran1, K. Bhatt1, J. F. Amatruda2, G. Danuser1 1UT Southwestern Medical Ctr, Dallas, TX, 2Children's Hospital Los Angeles, Dallas, TX
11:55 am MS178 Dynamically tuning the membrane lipid composition can control macromolecular assembly. J. A. Fischer, M. E. Johnson Johns Hopkins University, Baltimore, MD
12:00 pm MS179 clathrin dynamics in endothelial cells are impacted by shear stress. T. Nawara, E. Sztul, A. Mattheyses UNIVERSITY OF ALABAMA AT BIRMINGHAM, Birmingham, AL
Track(s): Cellular Dynamics; and Specialized Cell and Evolution
Room 207A
11:15 am MS180 High-dimensional morphology analysis enables identification and label-free enrichment of heterogeneous tumor cell populations. A. Jovic, K. Saini, T. Pham, C. Corona, J. Mei, M. Phelan, S. Boutet, K. B. Jacobs, J. Kim, C. Johnson, V. Lu, N. Li, M. Salek, M. Masaeli Deepcell, Inc, Menlo Park, CA
11:20 am MS181 Generating a Perfusable Vascularized Cerebral Organoid Model. E. LaMontagne1, L. J. Macdougall2, F. Papes1, E. L. Teng1, J. Mayner1, B. P. Sutherland3, K. S. Anseth2, A. M. Kloxin3, A. R. Muotri1, A. J. Engler1 1University of California, San Diego, La Jolla, CA, 2University of Colorado, Boulder, Boulder, CO, 3University of Delaware, Newark, DE
11:25 am MS182 Synthesis, characterization, and cellular imaging of a novel cyan emitting fluorescent α-amino acid. A. Gupta, B. Yan, M. Guo University of Massachusetts Dartmouth, Dartmouth, MA
11:30 am MS183 Entering the primary cilia of sensory neurons in C. elegans: A single-molecule perspective. A. Mitra, E. Loseva, E. J. G. Peterman Vrije Universiteit Amsterdam, Amsterdam, Netherlands
11:35 am MS184 Regulation of Human Neurofilament-light Assembly State by O-GlcNAc and Potential Hypoglycosylation of Charcot-Marie-Tooth Disease Mutants. D. T. Huynh, J. Hu, K. Tsolova, M. Zorawski, C.-C. Lin, E. Soderblom, N. Linhart, D. Li, J.-T. Chi, M. Boyce Duke University, Durham, NC
11:40 am MS185 The blood-brain barrier responds to inflammation differently in juvenile and adult brains. J. D. Ochrietor, A. Gonzalez, F. Laveus, J. Gelsleichter University of North Florida, Jacksonville, FL
11:45 am MS186 Lysosomal-dependent remodeling of endoplasmic reticulum-plasma membrane contacts drives neurodegeneration. M. Casas, K. Hino, N. Vierra, S. Simó, J. Trimmer, R. Dixon, E. Dickson University of California, Davis, Davis, CA
11:50 am MS187 Endocytic machinery are primed at neuronal synapses through enrichment of PI4P and PS at endocytic zones. B. Goulden, Y. Imoto, T. Ogunmowo Johns Hopkins, Baltimore, MD
11:55 am MS188 Selective axonal transport through branch junctions is directed by growth cone signaling and mediated by KIF1/kinesin-3 motors. S. Tymanskyj, L. Ma Thomas Jefferson University, Philadelphia, PA
12:00 pm MS189 Laser Delivery of Optogenetic Therapy in Murine Pain Models. A. Fijany1,2, D. Narcisse3, R. Benkowski2, A. Tripathy3, N. Al-Adli1, S. Mohanty3 1TCU School of Medicine, Fort Worth, TX, 2Designplex Biomedical, LLC, Fort Worth, TX, 3Nanoscope Technologies, LLC, Bedford, TX
Track(s): Cellular Dynamics; and Cellular Genome
Room 207B
11:15 am MS190 Persistently unaligned polar chromosomes link increased microtubule stability to biased aneuploidy landscape in tumor cells. K. Vukušić, S. Kodba, P. Risteski, I. M. Tolić Ruder Boskovic Institute, Zagreb, Croatia
11:20 am MS191 Modulating CENP-A Nucleosome Mobility Dictates Centromere Chromatin Accessibility. D. P. Melters1, K. C. Neuman2, T. Rakshit3, Y. Dalal1 1National Cancer Institute, Bethesda, MD, 2National Heart Lung and Blood Institute, Bethesda, MD, 3Shiv Nadar University, Dadri, India
11:25 am MS192 The Cell Cycle Modulates Active Stresses in Epithelial Tissue via Regulation of Junctional Mechanics. S. Mukherjee, J. Devany, M. L. Gardel University of Chicago, Chicago, IL
11:30 am MS193 Precision reproductive genetics: pinpointing the effects of a maternally-inherited Kif18a variant on oocyte meiosis and aneuploidy. L. Biswas1, M. Aboelenain1,2, S. Sun1, J. Xing1, K. Schindler1 1Rutgers University, New Brunswick, Piscataway, NJ, 2Mansoura University, Mansoura, Egypt
11:35 am MS194 Centromeric Chromatin Persists with No New Assembly in Oocytes over the Fertile Lifespan of Mice. A. Das1, K. G. Boese1, K. Tachibana2, B. E. Black1, M. A. Lampson1 1University of Pennsylvania, Philadelphia, PA, 2Max Planck Institute of Biochemistry, Munich, Germany
11:40 am MS195 Novel role of Translesion DNA synthesis polymerase kappa in the nucleolus and in nucleolar stress response recovery. S. Paul1, A. Heinbaugh1, A. Paul1, P. Cifani2, D. Pappin2, T. Spratt1 1Penn State University College of Medicine, Hershey, PA, 2Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
11:45 am MS196 Cip1 tunes cell cycle arrest duration upon calcineurin activation. M. J. Flynn, J. A. Benanti University of Massachusetts Chan Medical School, Worcester, MA
11:50 am MS197 In situ 3-D analysis of transcriptionally repressed yeast nuclei by cryo-ET. C. Y. Y. Chong, J. K. Chen, S. Cai, J. Shi, L. Gan National University of Singapore, Singapore, Singapore
11:55 am MS198 Pushing the envelope: force balance in fission yeast closed mitosis. M. A. Begley1, P. Zareiesfandabadi2, A. Sharma1, M. B. Rapp1, M. W. Elting1,1 1North Carolina State University, Raleigh, NC, 2Duke University, Durham, NC
12:00 pm MS199 Asymmetric cell division in human hematopoietic stem cells is regulated by cell-cell interaction. A. Candelas1,2, B. Vianay1,2, L. Blanchoin2,1, S. Brunet1,2, M. Thery1,2 1CytoMorpho Lab, Institut Pierre-Gilles de Gennes, PSL, Paris, France, 2CytoMorpho Lab, LPCV, CEA, Grenoble, France
Track(s): Cellular Dynamics; and Physical Cell
Room 201
11:15 am MS200 P4-ATPases Require Retromer for their Localization. M. Jimenez, J. T. Best, C. Kyoung, T. R. Graham Vanderbilt University, Nashville, TN
11:20 am MS201 Sulfolobus acidocaldarius adhesion pili power twitching motility in the absence of a dedicated retraction ATPase. A. Charles-Orszag1, M. van Wolferen2, S. J. Lord1, S.-V. Albers2, D. Mullins1 1University of California, San Francisco, San Francisco, CA, 2University of Freiburg, Freiburg, Germany
11:25 am MS202 Spatial transcriptomics reveals mechanically-regulated cell state transitions at the tumor-microenvironment interface. M. V. Hunter1, R. Moncada2, I. Yanai2, R. M. White1 1Memorial Sloan Kettering Cancer Center, New York, NY, 2NYU Langone Health, New York, NY
11:30 am MS203 A spatial map of hepatic mitochondria uncovers functional heterogeneity and plasticity at the tissue scale. S. Kang, R. P. Cunningham, C. Cultraro, A. Lobanov, M. Cam, N. Porat-Shlion National Instituts of Health, Bethesda, MD
11:35 am MS204 RhoA regulator-mediated control of cortical actomyosin patterning in the Drosophila egg chamber and embryo. J. A. Jackson1, M. Denk-Lobnig2, A. C. Martin1 1Massachusetts Institute of Technology, Cambridge, MA, 2University of Michigan – Ann Arbor, Ann Arbor, MI
11:40 am MS205 SGEF, a RhoG-specific GEF, Regulates Lumen Formation and Collective Cell Migration in 3D Epithelial Cysts. M. Lovejoy, A. Rabino, G. Kreider-Letterman, V. Gangasani, R. Garcia-Mata University of Toledo, Toledo, OH
11:45 am MS206 Diffusion-limited Dynamic Partitioning Can Define Cortical Asymmetry and Control Wave-like Spatiotemporal Patterning of Multiple Membrane Proteins. T. Banerjee1,2, S. Matsuoka3,4, Y. Miao1, Y. Kamimura4, D. Biswas2, D. S. Pal1, M. Ueda3,4, P. A. Iglesias2, P. N. Devreotes1 1Johns Hopkins University School of Medicine, Baltimore, MD, 2Johns Hopkins University, Baltimore, MD, 3Osaka University, Osaka, Japan, 4RIKEN Center for Biosystems Dynamics Research, Osaka, Japan
11:50 am MS207 Cdc42 Mobility and Membrane Flows Regulate Fission Yeast Cell Shape and Survival. D. M. Rutkowski1, V. Vincenzetti2, D. Vavylonis1, S. G. Martin2 1Lehigh University, Bethlehem, PA, 2University of Lausanne, Lausanne, SWITZERLAND.
11:55 am MS208 Endocytosis Regulates Negative Feedback to Promote Bipolar Growth in Fission Yeast. M. Harrell Boston College, Chestnut Hill, MA
12:00 pm MS209 Uncovering principles of cell organization using soft X-ray tomography and whole-cell integrated modeling of inter-organelle spatial relationships and physical interactions in S. cerevisiae. M. Mirvis, W. F. Marshall University of California, San Francisco, San Francisco, CA