Special Interest Subgroups

Subgroups are completely organized by members from session idea to execution, including session format, speaker selection, and schedule lineup. There will be a total of 27 Special Interest Subgroup sessions held during Cell Bio Virtual 2020. All sessions are 3 hours. View the full list of sessions below:

Subgroup Sessions and Schedule

Monday, December 7, 1:45 pm to 4:45 pm

Cells Migrating in Complex Environments

Scientific Tracks: Cells in Distress and Disease: Cancer, Aging, Infection, Stress, Chemical Biology, and Therapeutics, and Cellular Dynamics: Compartmentalization, Trafficking, Cytoskeleton, Division, and Migration

Organizers: Daria Siekhaus, Inst Science and Technology Austria;  David Sherwood, Duke University; and Andrew Ewald, Johns Hopkins University

The ability of cells to reach new positions in the body by crossing tissue or ECM barriers is required for normal development and immune cell control of infection as well as for cancer metastasis and new immuno-oncological approaches. We aim to bring the different communities together, from work in basic model organisms to studies on patient samples, to spur insights and collaboration on the cellular programs underlying this capacity across systems. We will highlight examinations of cellular programs and behaviors through the use of sophisticated imaging.

1:45 pm                              Introduction by Daria Siekhaus.

1:50 pm               SG1        Molecular cues controlling cell migration during germinal center responses. J. Cyster;  University of California, San Francisco, San Francisco, CA.

2:06 pm               SG2        Cancer-associated fibroblasts use supracellular contractility to compress and shape tumors. J. Barbazan1, C. Perez Gonzalez1, S. Richon1, X. Trepat2, D. Matic Vignjevic1;  1Institut Curie, Paris, FRANCE, 2IBEC, Barcelona, SPAIN.

2:22 pm               SG3        Epithelial cell division opens the door for macrophage tissue invasion in the Drosophila embryo. M. Akhmanova, D. E. Siekhaus;  IST Austria, Maria Gugging, AUSTRIA.

2:38 pm                              Break.

2:53 pm               SG4        Cell clusters adopt a collective amoeboid mode of migration in confined non-adhesive environments. F. Jaulin;  Gustave Roussy Institute, Villejuif, FRANCE.

3:09 pm               SG5        Localized glucose import and ATP production fuels cell invasion through basement membrane barriers. L. C. Kelley, I. Kenney, A. Garde, D. Sherwood;  Duke University, Durham, NC.

3:25 pm               SG6        Dissemination of RasV12-transformed Cells Requires the Mechanosensitive Channel Piezo. Y. Kwon, J. Lee, A. J. H. Cabrera, C. M. T. Nguyen;  Department of Biochemistry, University of Washington, Seattle, WA.

3:41 pm                              Break

3:56 pm               SG7        Epithelial-mesenchymal plasticity is required for metastasis in triple negative breast cancer models.. E. M. Grasset1, G. Sharma2, M. Dunworth1, S. Bracht1, M. Gentz1, E. J. Fertig3, A. J. Ewald1;  1Department of Cell Biology, Johns Hopkins University, Baltimore, MD, 2Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, 3Department of Oncology, Johns Hopkins University, Baltimore, MD.

4:12 pm               SG8        Investigating principles of homeostatic vascular remodeling via 4D imaging of live mice. C. Kam1, E. Marsh1, K. K. Hirschi2, V. Greco1;  1Department of Genetics, Yale University, New Haven, CT, 2Department of Cell Biology, University of Virginia, Charlottesville, CT.

4:28 pm               SG9        Compromised nuclear envelope integrity leads to TREX1-dependent DNA damage and tumor cell invasion. G. Nader1, S. Aguera-Gonzalez2, F. Routet2, M. Gratia3, M. Maurin2, V. Cancila4, C. Cadart2, M. Gentili3, A. Yamada5, C. Lodillinsky2, E. Lagoutte2, C. Villard6, J. Viovy6, C. Tripodo4, G. Scita7, N. Manel3, P. Chavrier2, M. Piel1;  1UMR144, Institut Curie and Institut Pierre Gilles de Gennes, Paris, FRANCE, 2UMR144, Institut Curie, Paris, FRANCE, 3U932, Institut Curie, Paris, FRANCE, 4University of Palermo-Tumor Immunology Unit, Palermo, ITALY, 5UMR168, Institut Curie, Paris, FRANCE, 6Institut Pierre Gilles de Gennes, Paris, FRANCE, 7IFOM, the FIRC Institute of Molecular Oncology, Milan, ITALY.

Cytoskeletal Ensembles Across Scales

Scientific Tracks: Cellular Dynamics: Compartmentalization, Trafficking, Cytoskeleton, Division, and Migration

Organizers: Jessica Feldman, Stanford University; Prachee Avasthi, University of Kansas Medical Center; Binyam Mogessie, University of Bristol; Stefanie Redemann, University of Virginia, School of Medicine; Sophie Dumont, University of California, San Francisco; Manu Prakash, Stanford University; and Alex Dunn, Stanford University

The cytoskeleton is a highly adaptable network of polymers that can dramatically reorganize to drive cell transformations and functions. To perform these functions, cytoskeletal polymers can act alone or join together across a range of contexts and scales. The resulting cytoskeletal ensembles range from macromolecular structures, local networks of different polymers that crosstalk, and large scale networks that form cellular machines. We will explore these topics, discussing emerging concepts and approaches in this subgroup session focused on highlighting the work of a diverse group of trainees and labs.

1:45 pm                              Introduction by Alex Dunn, Manu Prakash, and Sophie Dumont.

1:47 pm               SG10      Intertwined orthogonal contractile fibers across heart ventricular walls. D. Dileep1, T. Syed2, K. Siddiqi2, M. Sirajuddin1;  1INSTEM, Bangalore, Bangalore, INDIA, 2McGill University, Montreal, QC, CANADA.

1:59 pm               SG11      Reconstitution of Excitable and Oscillatory Rho Waves. J. Landino1, M. Leda2, A. Michaud3, Z. Swider3, W. M. Bement3, A. G. Vecchiarelli1, A. B. Goryachev2, A. L. Miller1;  1University of Michigan, Ann Arbor, MI, 2University of Edinburgh, Edinburgh, UNITED KINGDOM, 3University of Wisconsin, Madison, WI.

2:11 pm               SG12      Visco-elastic properties of bulk cytoplasm maintain the mitotic spindle in the center of large cells. J. Xie, J. Najafi, J. Sallé, N. Minc;  Institut Jacques-Monod, Paris, FRANCE.

2:23 pm               SG13      Opposing motors provide mechanical and functional robustness in the mammalian spindle. L. Neahring1,2, S. Dumont1,2,3,4;  1DSCB Graduate Program, UCSF, San Francisco, CA, 2Dept of Bioengineering & Therapeutic Sciences, UCSF, San Francisco, CA, 3Dept of Biochemistry & Biophysics, UCSF, San Francisco, CA, 4Chan Zuckerberg Biohub, San Francisco, CA.

2:35 pm                              Break.

2:50 pm                              Introduction by Jessica Feldman and Prachee Avasthi.

2:52 pm               SG14      Visualizing Microtubule Array Remodeling at Single Microtubule and Protofilament Resolution by Atomic Force Microscopy. R. Subramanian;  Harvard Medical School and MGH, Boston, MA.

3:04 pm               SG15      Actin cables, clouds, and comet tails organize mitochondrial networks in mitosis. A. S. Moore1, S. M. Coscia2, F. E. Ortega3, J. A. Theriot4, J. Lippincott-Schwartz1, E. L. F. Holzbaur2;  1Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, 2Department of Physiology, University of Pennsylvania, Philadelphia, PA, 3Department of Biochemistry, Stanford University, Stanford, CA, 4Department of Biology, University of Washington / HHMII, Seattle, WA.

3:16 pm               SG16      Asymmetric Inheritance of Keratin Intermediate Filaments Regulates Fate in the Early Mammalian Embryo. G. Lim1, Y. D. Alvarez1, M. Gasnier1, Y. Wang2, P. Tetlak1, S. Bissiere1, H. Wang2, M. Biro3, N. Plachta1;  1Institute of Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR), Singapore, SINGAPORE, 2State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, CHINA, 3EMBL Australia, Single Molecule Science Node, School of Medical Sciences, University of New South Wales, Sydney, AUSTRALIA.

3:28 pm               SG17      Anchoring of cortical actin pools by the late endocytic pathway during subcellular tube guidance. D. Rios1,2, M. Leptin2,3;  1Institute for Biomedical Research, National Autonomous University of Mexico, Mexico City, MEXICO, 2Directors' Research, European Molecular Biology Laboratory, Heidelberg, GERMANY, 3Institute for Genetics, University of Cologne, Cologne, GERMANY.

3:40 pm                              Break.

3:55 pm                              Introduction by Binyam Mogessie and Stefanie Redemann.

3:57 pm               SG18      The Drosophila spectraplakin Short stop integrates cell-matrix adhesion during cell migration. A. Zhao1, J. Montes-Laing1, W. M. Perry1, M. S. Cobb1, E. Merfeld1, S. L. Rogers2, D. A. Applewhite1;  1Biology, Reed College, Portland, OR, 2Biology & Carolina Center for Genome Sciences, University of North Carolina Chapel Hill, Chapel Hill, NC.

4:09 pm               SG19      Septins mediate actin filament capture and polymerization along microtubules. K. Nakos1, M. R. Radler1, S. B. Padrick2, E. T. Spiliotis1;  1Department of Biology, Drexel University, Philadelphia, PA, 2Department of Biochemistry and Molecular Biology, Drexel University, Philadelphia, PA.

4:21 pm               SG20      Reciprocal regulation between cytoskeletal elements and microRNA-31 during cell division. K. Konrad, C. Remsburg, J. L. Song;  University of Delaware, Newark, DE.

4:33 pm               SG21      Tropomyosin/actin filaments are required for merging of microtubule asters in mitotic spindle assembly. Y. Wang1, X. Xu1, J. H. Stear1, N. Bryce1, M. Carnell2, E. C. Hardeman1, P. W. Gunning1;  1Cellular and Genetic Medicine Unit, School of Medical Sciences, University of New South Wales, Sydney, AUSTRALIA, 2Biomedical Imaging Facility, Mark Wainwright Analytical Center, University of New South Wales, Sydney, AUSTRALIA.

Reconstituting Cell Biology

Scientific Tracks: Cellular Dynamics: Compartmentalization, Trafficking, Cytoskeleton, Division, and Migration, and Physical Cell: Bioengineering, Mechanobiology, and Synthetic Biology

Organizers: Laurent Blanchoin, Grenoble at the CEA; Gary Brouhard, McGill University; Daniel Fletcher, UC Berkeley; Matthew Good, University of Pennsylvania; and Kassandra Ori-McKenney, University of California, Davis

Reconstitution of sophisticated biological processes from their component molecular parts has emerged as a powerful and broadly useful tool in cell biology. These experiments have revealed the basic mechanisms that guide the assembly of cytoskeletal-based structures, underlie cytoskeletal-based transport, shape membrane-bound and membrane-less compartments, and organize multicellular tissues. Rapid progress in micropatterning, microfluidics, and microfabrication, coupled with continued advancements in biochemistry and molecular biology, have created the opportunity for more complete cellular reconstitutions that may one day rival the complexity of live cells or tissues. Building on its roots in biochemical reconstitution of enzyme activity, modern reconstitution efforts demonstrate that cell and tissue-scale organization can be assembled from nanometer-scale parts by combining purified proteins and cytoplasmic extracts with cell-like boundary conditions, including dynamic lipid membranes. By identifying the necessary and sufficient conditions for assembly, these ‘bottom-up’ studies provide new mechanistic insight that complements more traditional ‘top-down’ cell biology. This session will feature a selection of the most ambitious and cutting-edge examples of biochemical and cellular reconstitution.

1:45 pm                              Introduction.

1:47 pm               SG22      The limits to (actin) growth. P. Bieling;  Department of Systemic Cell Biology, Max Planck Insitute for Molecular Physiology, Dortmund, GERMANY.

2:02 pm               SG23      Filament nucleation tunes mechanical memory in actomyosin networks. V. Yadav1, D. Banerjee2, A. Tabatabai1, D. Kovar3, T. Kim4, S. Banerjee2, M. Murrell1;  1Yale University, New Haven, CT, 2Carnegie Mellon, Pittsburgh, PA, 3University of Chicago, Chicago, IL, 4Purdue University, West Lafayette, IN.

2:17 pm               SG24      Spatial control of membrane traffic - deciphering and reconstituting the septin GTPase code. E. T. Spiliotis, A. Suber, E. P. Karasmanis, K. Nakos;  Drexel University, Philadephia, PA.

2:32 pm               SG25      Microtubule lattice defects promote catastrophes. A. Akhmanova1, A. Rai1, T. Liu2, J. Estévez-Gallego3, E. A. Katrukha1, F. Díaz3, L. C. Kapitein1, C. A. Moores2;  1Biology, Utrecht University, Faculty of Science, Utrecht, NETHERLANDS, 2Birkbeck, University of London, London, UNITED KINGDOM, 3Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas, Madrid, SPAIN.

2:47 pm               SG26      Collective effects of XMAP215, EB1, CLASP2, and MCAK lead to robust microtubule treadmilling. G Arpag, E. J. Lawrence, V. J. Farmer, S. L. Hall, M. Zanic;  Vanderbilt University, Nashville, TN.

3:02 pm               SG27      Structural and material design principles of centrosomes. J. Woodruff;  Cell Biology and Biophysics, UT Southwestern Medical Center, Dallas, TX.

3:17 pm                              Break

3:32 pm               SG28      Reconstitution of mitotic chromosome scaling using Xenopus egg extracts. C. Y. Zhou1, B. Dekker2, J. Dekker2, R. Heald1;  1Molecular and Cell Biology, UC Berkeley, Berkeley, CA, 2University of Massachusetts Medical School, Worchester, MA.

3:46 pm               SG29      Controlled Metabolic Cascades for the Protein Synthesis in an Artificial Cell. K. Shin;  Chemistry and Institute of Biological Interfaces, Sogang University, Seoul, KOREA, REPUBLIC OF.

4:01 pm               SG30      Mechanism of ER-associated protein degradation (ERAD). T. Rapoport;  Cell Biology, Harvard Medical Sch/HHMI, Boston, MA.

4:16 pm               SG31      Structural and molecular determinants of dynamics in P granules of C. elegans.. S. Jelenic1, J. Bindics1, A. S. Holehouse2, M. Ruer3, P. Czermak1, R. V. Pappu2, A. A. Hyman3, S. Saha1;  1Institute of Molecular Biotechnology (IMBA), Vienna, AUSTRIA, 2Washington University, St. Louis, MO, 3Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, GERMANY.

4:30 pm               SG32      Surviving stem cell injury. K. McKinley1, C. Trentesaux1, R. Zwick1, D. Boffelli1, J. Bush1, F. de Sauvage2, O. Klein1, R. Vale1;  1UCSF, San Francisco, CA, 2Genentech, San Francisco, CA.

Remodeling and Reshaping Membranes

Scientific Tracks: Cellular Dynamics: Compartmentalization, Trafficking, Cytoskeleton, Division, and Migration, and Specialized Cell and Evolution: Neurobiology, Immunology, and Emerging Model Systems  

Organizers: Gautam Dey, University College London;  Ishier Raote, Centre for Genomic Regulation, Barcelona; and Aurélien Roux, Biochemistry Department, University of Geneva

A small number of protein families, many traceable through 2 billion years of evolutionary time to the common ancestors of all eukaryotes, together shape lipid membranes into the breathtaking diversity of dynamic compartments and structures that define eukaryotic cells. Biochemistry and structural studies have revealed the physicochemical basis for the ability of proteins to shape, bend, and remodel membranes in model systems; phylogenetics and structural modelling have mapped the changes that link orthologs across evolutionary time; genetics and microscopy have anchored this understanding within a sub-cellular and organismal context.

In this session, we will bring together leaders in their respective fields: biochemists, evolutionary biologists, cell biologists and developers of emerging technologies. In bringing these disparate strands of research together, blending ancestral reconstruction, in vitro reconstitution, cell biology in new model systems, and theoretical modelling to answer key questions, we hope to help boost the field of “evolutionary biochemistry” (Harms, Thornton 2013) How does a protein domain adjust to an evolving cellular context and functional niche? How does sequence evolution influence protein function? How does protein architecture or folding constrain sequence evolution? Addressing such questions will lead to a better understanding of how and why biological molecules have the properties that they do - shedding light on the key membrane remodelling processes that power cellular life.

1:45 pm               SG33      Mechanism of ER-associated organelle division. G. Voeltz;  HHMI/University of Colorado-Boulder, Boulder, CO.

1:58 pm               SG34      LEM2 phase separation promotes ESCRT-mediated nuclear envelope reformation.. A. Von Appen;  UCSF, San Francisco, CA.

2:11 pm               SG35      Regulation of organelle remodelling by the ESCRT machinery. J. Carlton, A. Gatta, G. Pearson, C. Stoten;  King's College London and Francis Crick Institute, London, UNITED KINGDOM.

2:24 pm               SG36      Structure of the yeast seipin Fld1 reveals determinants for LD biogenesis. P. Carvalho, Y. A. Klug, J. C. Deme, S. M. Lea;  Sir William Dunn School of Pathology, University of Oxford, UNITED KINGDOM.

2:37 pm                              Break

2:49 pm               SG37      mechanisms directing the morphology of the early secretory pathway. A. M. Ernst;  Division of Biological Sciences, Section of Cell & Developmental Biology, University of California, San Diego, La Jolla, CA.

3:02 pm               SG38      Membrane Fission: Insights from Reconstituting Organelle Form and Chemistry. T. Pucadyil;  IISER Pune, Pune, INDIA.

3:15 pm               SG39      Structure of the complete, membrane-assembled COPII coat reveals a complex interaction network.. G. Zanetti;  ISMB, Birkbeck College, London, UNITED KINGDOM.

3:28 pm               SG40      Membrane shaping proteins control lamellipodia dynamics and cell migration. E. Sitarska, S. Dias Almeida, A. Diz-Muñoz;  Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, GERMANY.

3:41 pm               SG41      Membrane flows pattern the distribution of membrane-associated proteins around sites of secretion. S. G. Martin1, V. Gerganova1, I. Lamas1, A. Vjestica1, D. Rutkowski2, D. Vavylonis2;  1University of Lausanne, Lausanne, SWITZERLAND, 2Lehigh University, Bethlehem, PA.

3:54 pm                              Break

4:07 pm               SG42      Adaptor protein complexes: ancient sorting machines. M. S. Robinson, J. Hirst;  CIMR/University of Cambridge, Cambridge, UNITED KINGDOM.

4:20 pm               SG43      Multiscale modeling of membrane bending. H. Alimohamadi, G. Angles, J. Gan, C. Lee, A. Mahapatra, C. Zhu, P. Rangamani;  UCSD, La Jolla, CA.

4:33 pm               SG44      Ancient trafficking machinery lost in animals and fungi: Rise of the Jotnarlogs. J. B. Dacks;  Medicine, University of Alberta, Edmonton, AB, CANADA.

Single-Cell Signaling and Regulatory Networks in Cell Biology

Scientific Track: Signaling and Metabolism: Integrating Intra- and Intercellular Signaling, and Information Processing

Organizers: Timothy Aikin, Johns Hopkins University; Sergei Regot, Johns Hopkins University; Jennifer Bailey, UC Davis; Abhineet Ram, UC Davis; Jing Chen, Virginia Tech; Christian Hong, University of Cincinnati; and Silke Hauf, Virginia Tech

Cellular decision making relies upon precise transfer of information in the form of biochemical signals. While the components of many signaling pathways are known, single-cell reporters are revealing complex and heterogeneous signaling states in individual cells. Patterns of signaling over time, or dynamics, are regulated by molecular networks with intricate circuit designs and interlocking feedback loops, which can only be understood through theoretical modeling. For single cells, these dynamics inform key behaviors, such as migration, proliferation, metabolism, apoptosis, and differentiation. Ultimately, signaling dynamics underlie cellular decision making during development, homeostatic regulation, and response to stress and disease. Through the integration of experimental tools and computational approaches, we can interrogate the ways in which signaling dynamics are generated and regulated during diverse cellular processes. This session will explore novel signaling biosensors, quantitative experimental approaches, and theoretical frameworks for studying the regulatory networks of single cells, and the ways in which dynamics inform cell behaviors.

1:45 pm                              Introduction and Honoring of John Tyson's Retirement.

1:49 pm               SG45      Temporal organization of the cell cycle. B. Novak;  University of Oxford, Oxford, UNITED KINGDOM.

2:06 pm               SG46      Combining optogenetics with network perturbations highlights different regulatory mechanisms of the ERK signaling pathway. C. Dessauges1, J. Mikelson2, M. Dobrzynski1, M. Jacques1, M. Khammash2, O. Pertz1;  1Institute of Cell Biology, University of Bern, Bern, SWITZERLAND, 2Department of Biosystems Science and Engineering, ETH Zürich, Basel, SWITZERLAND.

2:14 pm               SG47      Studying signaling dynamics without live-cell microscopy. P. T. Ravindran1, S. E. McFann1, J. E. Toettcher2;  1Chemical and Biological Engineering, Princeton University, Princeton, NJ, 2Molecular Biology, Princeton University, Princeton, NJ.

2:31 pm               SG48      A switch in p53 dynamics marks cells that escape from DSB-induced cell cycle arrest. M. Tsabar1, C. S. Mock1, V. Venkatachalam1, J. Reyes2, K. W. Karhohs1, T. G. Oliver3, A. Regev4, A. Jambhekar1, G. Lahav1;  1Systems Biology, Harvard Medical School, Boston, MA, 2Memorial Sloan Kettering, New York, NY, 3Huntsman Cancer Institute, University of Utah, Salt Lake City, UT, 4Broad Institute, Cambridge, MA.

2:39 pm               SG49      Our first choices - decoding signals during developmental transitions. S. Santos;  The Francis Crick Institute, London, UNITED KINGDOM.

2:56 pm               SG50      Integrating decades of data into a predictive computational model of RTK-dependent gene regulation. S. Dutta1, A. L. Patel1, S. E. Keenan1, S. Y. Shvartsman1,2;  1Princeton University, Princeton, NJ, 2Center for Computational Biology, Flatiron Institute, New York, NY.

3:04 pm                              Break

3:08 pm               SG51      Models for intracellular signaling in cell motility. L. Edelstein-Keshet;  University of British Columbia, Dept of Mathematics, Vancouver, BC, CANADA.

3:25 pm               SG52      Nutrient signaling, stress response, and interorganelle communication are noncanonical determinants of cell fate. E. Wood;  UT Southwestern Medical Center, Dallas, TX.

3:33 pm               SG53      Why cells arrest when stress (and why you should give a "flux" about it). H. El-Samad, A. Bonny;  University of California, San Francisco, San Francisco, CA.

3:50 pm               SG54      Signaling adaptation mediates rapid escape from BRAF inhibition in single melanoma cells. C. Tian, C. Yang, T. Hoffman, N. Jacobsen, S. Spencer;  University of Colorado Boulder, Boulder, CO.

3:58 pm               SG55      Mechanisms for robust circadian rhythms against spatial stochastic cellular kinetics. J. Kim;  Korea Advanced Institute of Science and Technology, Daejeon, KOREA, REPUBLIC OF.

4:15 pm                              Q&A Session and Closing Remarks

The Nuclear Envelope and Nucleoporins: Influencers of Cellular Structure and the Genome

Scientific Tracks: Physical Cell: Bioengineering, Mechanobiology, and Synthetic Biology, and Cellular Genome: 4D Organization, Expression, Replication, and Repair

Organizers: Eda Yildirim, Duke University Sch Med; Katharine Ullman, University of Utah; Lori Wallrath, University of Iowa; and Jan Lammerding, Cornell University

There is growing interest in the nuclear envelope, as it plays key roles in the transmission and translation of information from the cytoplasm to the genome through the action of biochemical signals and mechanical forces. The constituents of the nuclear envelope, i.e., nuclear membrane proteins, lamins, and nucleoporins, which form the building blocks of nuclear pore complexes (NPCs), have far-reaching roles in regulating the cellular genome and cellular function. This Special Interest Subgroup will unite research on diverse biological roles of nuclear envelope and nuclear pore proteins and identify novel connections among their physical, signaling, and genome organizational properties. Examples include the ways in which these proteins influence response to biomechanical forces (mechanobiology), modulate cell migration, and support tissue-specific functions. Research in this arena provides insight into fundamental cell biology and is beginning to provide a comprehensive context to understand how nucleoporins and nuclear envelope proteins are causally linked to a range of human diseases, viral infections and cancer.

1:45 pm               SG56      Models and mechanisms of mechanotransduction across the nuclear envelope. M. C. King, I. V. Surovtsev, J. F. Williams, H. Nguyen, E. Carley, I. Jalilian;  Department of Cell Biology, Yale School of Medicine, New Haven, CT.

2:00 pm               SG57      Alterations to nuclear pore complexes occur with force driven localization to Actin-LINC-Lamin nuclear lines. M. A. Smith1,2, E. Blankman3,2, L. M. Hoffman1,2, C. C. Jensen3,2, K. S. Ullman3,2, M. C. Beckerle3,2;  1Biological Sciences, University of Utah, Salt Lake City, UT, 2Huntsman Cancer Institute, Salt Lake City, UT, 3Oncological Sciences, University of Utah, Salt Lake City, UT.

2:15 pm               SG58      Actin assembly ruptures the nuclear envelope by prying the lamina away from nuclear pores and nuclear membranes in starfish oocytes. N. Wesolowska1, I. Avilov2, P. Machado3, C. Geiss1, H. Kondo1, M. Mori1, P. Lenart1,2;  1European Molecular Biology Laboratory, Heidelberg, GERMANY, 2Max Planck Institute for Biophysical Chemistry, Göttingen, GERMANY, 3Electron Microscopy Core Facility, European Molecular Biology Laboratory (EMBL), Heidelberg, GERMANY.

2:30 pm                              Break

2:45 pm               SG59      Nup98-dependent transcriptional memory is established independently of transcription.. P. Pascual-Garcia, S. Little, M. Capelson;  University of Pennsylvania, Philadelphia, PA.

3:00 pm               SG60      Transcription and chromatin architecture regulation by nucleoporin proteins. S. Kadota, J. Ou, Y. Shi, J. Sun, E. Yildirim;  Cell Biology, Duke University School of Medicine, Durham, NC.

3:15 pm               SG61      Nuclear pore complex quality control in neurodegeneration. A. N. Coyne1, B. Zaepfel1, P. Lusk2, J. D. Rothstein1;  1Johns Hopkins University School of Medicine, Baltimore, MD, 2Yale School of Medicine, New Haven, CT.

3:30 pm               SG62      Evidence that Hutchinson-Gilford progeria syndrome is driven by toxic progerin protein accumulation specifically within the cardiovascular system.. J. Hasper1, K. Welle2, S. Ghaemmaghami2, A. Buchwalter1;  1University of California, San Francisco, San Francisco, CA, 2University of Rochester, Rochester, NY.

3:45 pm                              Break

4:00 pm               SG63      LINC complex disruption suppresses laminopathies. C. L. Stewart;  Instute of Medical Biology, Singapore, SINGAPORE.

4:15 pm               SG64      Emerin expression rescues nuclear structure in invasive breast cancer cells to inhibit cell migration and metastasis. A. Liddane1,2, M. Campbell1, I. Mercier1, J. Holaska2;  1The University of the Sciences, Philadelphia, PA, 2Department of Biomedical Sciences, Cooper Medical School of Rowan University, Camden, NJ.

4:30 pm               SG65      Modulating nuclear pore complex numbers for cancer therapies. S. Sakuma, M. Raices, J. Borlido, V. Guglielmi, E. Y. S. Zhu, M. D'Angelo;  Sanford Burnham Prebys Medical Discovery Institute, Sanford Burnham Prebys Medical Discovery Institute, CA.

When Branches of the Tree of Life Meet: Cell Interactions Between Organisms

Scientific Tracks: Specialized Cell and Evolution: Neurobiology, Immunology, and Emerging Model Systems, and Communal Cell: Development, Differentiation, Regeneration, Stem Cells, Organs, and Organoids

Organizers: Vincent Boudreau, University of California, San Francisco; Jennifer Heppert, University of Tennessee-Knoxville

Cells never live in isolation and often participate in intimate interactions with both partners and foes across multiple kingdoms. These associations present opportunities to uncover novel specialized and generalized mechanisms of cell-cell interactions. Touching on classical cell biological themes including cell structure, adhesion, motility and immunity, this subgroup will explore how seemingly independent organisms associate, resulting in emergent biological function. Requiring the use of diverse approaches to probe these cellular relationships, this session will be highly interdisciplinary incorporating advanced microscopy, mathematical modelling, ecophysiology, -omics and a breadth of model systems. Featured speakers will contribute to the common goal of understanding how kingdoms of life are interwoven on a cellular level.

1:45 pm                              Introduction

1:55 pm               SG66      Cooperation and conflict in multicellular bacterial aggregates. J. Schwartzman1, A. Ebrahimi1, Y. Sato1, G. Chadwick2, V. Orphan2, O. Cordero1;  1Massachusetts Institute of Technology, Cambridge, MA, 2California Institute of Technology, Pasadena, CA.

2:10 pm               SG67      EEEnteroendocrine cells sense bacterial tryptophan catabolites to activate enteric and vagal neuronal pathways. L. Ye, R. Liddle, J. Rawls;  Duke University, Durham, NC.

2:25 pm               SG68      The Stimulation of Tubeworm Metamorphosis by a Bacterial Injection System. N. Shikuma;  San Diego State University, San Diego, CA.

2:40 pm                              Break

2:55 pm               SG69      A bacterial protein promotes membrane fusion during Chlamydia infection. F. Paumet, J. Wesolowski;  Microbiology and Immunology, Thomas Jefferson University, Philadelphia, PA.

3:10 pm               SG70      Host factors induce a rapid developmental transition in the parasitic chytrid fungus Batrachochytrium dendrobatidis. K. Robinson, L. Fritz-Laylin;  Biology, University of Massachusetts Amherst, Amherst, MA.

3:25 pm               SG71      From endosymbionts to organelles: genetic, biochemical, and cell biological integration of bacteria into host cells. J. McCutcheon;  Arizona State University, Tempe, AZ.

3:40 pm                              Break

3:55 pm               SG72      Cooperation and Conflict in a Social Fungus. N. GLASS;  University of California, Berkeley, CA.

4:10 pm               SG73      Choanoflagellates and the origin of animal innate immunity. A. Woznica;  Microbiology, UT Southwestern Medical Center, Dallas, TX.

4:25 pm               SG74      Dinoflagellate symbionts escape vomocytosis by suppressing immunity in coral host cells. A. Guse, M. Jacobovitz, S. Rupp, P. Voss, S. Gornik;  Centre for Organismal Studies, Heidelberg University, Heidelberg, GERMANY.

4:40 pm                              Closing Remarks

Tuesday, December 8, 1:45 pm to 4:45 pm

Building the Cell - 20 Years After

Scientific Tracks: Cellular Dynamics: Compartmentalization, Trafficking, Cytoskeleton, Division, and Migration, and Physical Cell: Bioengineering, Mechanobiology, and Synthetic Biology

Organizers: Susanne Rafelski, Allen Institute for Cell Science; and Wallace Marshall, UC San Francisco

Modern cell biology has made great strides in understanding cell structure and function. As with any engineering problem, however, there is a third important aspect that needs to be understood besides structure and function, and that is assembly. How are the complex three-dimensional structures found within the cell specified by a one-dimensional genome? In this session we will explore the mechanisms by which cellular structures are determined and regulated. Because this question lies at the interface of biology and physics, this Building the Cell session will be highly interdisciplinary with speakers whose interests range from physics and mathematical modeling to biochemistry and cell biology. This is also a special “20 years after” anniversary session in which we invite some of the first speakers from the first years of this subgroup to see how the field has evolved over the past 20 years.

1:45 pm                              Introduction

1:47 pm               SG75      Building the cytoskeleton: How do the building blocks of biological polymers combine to produce the spectrum of behaviors displayed by actin, tubulin, and their relatives?. H. Goodson1, A. Mauro1, J. Scripture1, K. Murray1, S. Mahserejian2;  1Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, IN, 2Pacific Northwest National Laboratory, Richland, WA.

2:05 pm               SG76      Turnover, mechanics, and structural plasticity of lamellipodial actin networks. D. Holz, D. M. Rutkowski, A. R. Hall, D. Vavylonis;  Lehigh University, Bethlehem, PA.

2:23 pm               SG77      How immune cells respond to physical cues - the role of cytoskeletal dynamics. A. Upadhyaya;  University of Maryland, College Park, MD.

2:41 pm               SG78      Chromosome clustering excludes cytoplasm during nuclear assembly. M. Petrovic*1, S. Cuylen-Haering*1,2, A. Hernandez-Armendariz2,3, M. W. G. Schneider1, M. Samwer1, C. Blaukopf1, L. J. Holt4, D. W. Gerlich1;  1Institute of Molecular Biotechnology of the Austrian Academy of Sciences, Vienna BioCenter, Vienna, AUSTRIA, 2European Molecular Biology Laboratory (EMBL), Heidelberg, GERMANY, 3Collaboration for joint PhD degree between EMBL and Heidelberg University, Faculty of Biosciences, Heidelberg, GERMANY, 4Institute for Systems Genetics, New York University Langone Health, New York, NY.

2:56 pm               SG79      Life in a crowded environment. F. Chang1, P. Odermatt1, K. C. Hwang2;  1UCSF, San Francisco, CA, 2Stanford, Stanford, CA.

3:14 pm                              Break

3:39 pm               SG80      Robustness and universal scaling in endomembrane organelle size control. K. Panjtan Amiri1, A. Kalish1, S. Mukherji2;  1Physics, Washington University in St. Louis, St Louis, MO, 2Physics/Cell Biology & Physiology, Washington University in St. Louis, St Louis, MO.

3:54 pm               SG81      Decoding the variance in integrated intracellular organization of the undifferentiated hiPS cell. M. P. Viana1, J. A. Theriot2, S. M. Rafelski1;  1Allen Institute for Cell Science, Seattle, WA, 2University of Washington, Seattle, WA.

4:12 pm               SG82      Mechanical constraints play a critical role in protein segregation and morphogenesis in the early C. elegans embryo. A. Dawes1, J. de l'Etoile2;  1Mathematics/Molecular Genetics, Ohio State University, Columbus, OH, 2Biophysics, Ohio State University, Columbus, OH.

4:30 pm               SG83      Entropy and mechanics drive tissue formation and breakdown. V. Srivastava1, J. Hu1, m. labarge1, M. Thomson2, J. Garbe1, Z. J. Gartner1;  1University of California, San Francisco, San Francisco, CA, 2California Institute of Technology, Pasadena, CA.

Cell Polarity Signaling in Neurons

Scientific Tracks: Specialized Cell and Evolution: Neurobiology, Immunology, and Emerging Model Systems

Organizers: Huaye Zhang, Rutgers University;  Yimin Zou, University of California San Diego

Neurons are among the most highly polarized cell types in the human body. Yet when it comes to cell polarity mechanisms, it has become increasingly clear that neurons are not “just another cell type”.  While some polarity signaling pathways are shared, others are neuronal-specific.  Thus it is important to understand these mechanisms within the context of neurons.  This special interest subgroup will bring together researchers interested in cell polarity mechanisms in the subcellular compartmentalization of neurons. Topics will include dendritic-axonal compartmentalization, trafficking, synapse formation, bipolar polarity, and planer cell polarity.

1:45 pm                              Introduction by Huaye Zhang and Yimin Zou

1:50 pm               SG84      Oxygen-tension and the VHL-Hif1 pathway determine onset of neuronal polarization and cerebellar germinal zone exit. D. Solecki;  St. Jude Children's Res Hosp, Memphis, TN.

2:06 pm               SG85      A multi-compartment neuron reveals differences between apical-basal and axon-dendrite sorting signals. M. Lillis, N. Zaccardi, M. G. Heiman;  Harvard Medical School, Boston Children's Hospital, Boston, MA.

2:22 pm               SG86      Neuronal polarity requires dendritic protein endocytosis in the axon initial segment. K. Eichel1, T. Uenaka1, S. Cheng2, J. Pak2, C. A. Taylor1, M. Wernig1, K. Shen1;  1Stanford University, Stanford, CA, 2University of Chicago, Chicago, IL.

2:38 pm               SG87      Degradative regulation of dendritic cargos. C. Yap, L. Digilio, L. McMahon, I. Witteveen, B. Winckler;  University of Virginia, University of Virginia, Charlottesville VA, VA.

2:54 pm                              Break

2:59 pm               SG88      A quality control system couples microtubule nucleation and polarity at dendrite branch points. C. Feng1, J. Cleary2, W. Hancock2, M. Rolls1;  1Biochemistry and Molecular Biology, Pennsylvania State University, University Park, PA, 2Biomedical Engineering, Pennsylvania State University, University Park, PA.

3:15 pm               SG89      Semaphorin3A and PlexinA3 association with a scaffold for cGMP increase is required for apical dendrite development. J. Szczurkowska, A. Guo, M. Shelly;  Neurobiology and Behavior, Stony Brook University, Stony Brook, NY.

3:31 pm               SG90      Par polarity proteins in dendritic spine morphogenesis and plasticity. E. C. Kelly-Castro, Q. Wu, M. Sun, H. Zhang;  Rutgers University, Piscataway, NJ.

3:47 pm                              Break

3:57 pm               SG91      Planar cell polarity signaling in growth cone guidance and synapse formation. Y. Zou;  University of California, San Diego, La Jolla, CA.

4:13 pm               SG92      Planar Cell Polarity Signaling Directs Growth Cone Guidance Through Non-Autonomous Mechanisms Acting in Target Tissues. M. R. Deans;  Surgery / Otolaryngology, University of Utah School of Medicine, Salt Lake City, UT.

4:29 pm               SG93      Investigating circuit mechanisms for CELSR3 and the pathophysiology of Tourette Disorder in genetic mouse models. M. Tischfield;  Cell Biology and Neuroscience, Rutgers University, Child Health Institute, New Brunswick, NJ.

Centromere Structure and Function

Scientific Track: Cellular Genome: 4D Organization, Expression, Replication, and Repair

Organizers: Fei Li, Department of Biology, New York University

Centromeres guide the assembly of kinetochores to ensure proper segregation of chromosomes during mitosis and meiosis. Dysfunctional centromeres have long been associated with diseases, including cancer. The study of centromeres has led to major leaps in our understanding of chromosome segregation, epigenetic mechanisms, cell cycle regulation, chromosome evolution, and disease. The centromere field has recently made rapid progress by exploiting the latest technical advances. These new technologies enable groundbreaking discoveries in centromere biology, and pave the way towards potential novel therapies resulting from mis-regulation of centromeres. This subgroup will bring together researchers across the globe and spanning career stages to present and discuss the latest exciting findings on the fundamentals of centromere biology as well as new concepts within the field, ranging from centromere inheritance to centromere evolution. We will approach these themes in a variety of model organisms. The role of centromeres in physiology and disease will also be highlighted.

1:45 pm               SG94      Elucidating the Dynamics and Regulation of Kinetochore Assembly. A. Popchock1, J. Larson2, C. Asbury2, S. Biggins1;  1Fred Hutchinson Cancer Res Ctr, Seattle, WA, 2University of Washington, Seattle, WA.

2:03 pm               SG95      Epigenetic, genetic, and sex-specific facets of centromere identity and strength. B. E. Black;  Biochemistry & Biophysics, University of Pennsylvania, Philadelphia, PA.

2:21 pm               SG96      Holocentromere formation without centromeric histone variants in Lepidoptera avoids active chromatin. A. Senaratne, N. Cortes-Silva, H. Muller, I. Drinnenberg;  Institut Curie, Paris, FRANCE.

2:39 pm                              Break

2:54 pm               SG97      Roles of novel kinesin-14s in maize meiotic drive. K. Dawe;  University of Georgia, Athens, GA.

3:12 pm               SG98      Investigating the functional conservation of centromeric retroelements in Drosophila. B. Santinello1, A. Chavan1, J. Palladino1, L. Ouyang1, A. Amjad1, C. Chang2, X. Wei3, C. Courret2, N. Martins4, C. Wu4, A. Larracuente2, B. Mellone1;  1University of Connecticut, Storrs, CT, 2University of Rochester, Rochester, NY, 3University of Rochester Medical Center, Rochester, NY, 4Harvard Medical School, Boston, MA.

3:30 pm               SG101    A genetic memory initiates the epigenetic loop necessary to preserve centromere position. S. Hoffmann, D. Fachinetti;  Institut Curie, Paris, FRANCE.

3:48 pm                              Break

4:03 pm               SG100    Cell cycle control of centromere assembly. J. Servin, A. Straight;  Stanford Medical School, Stanford, CA.

4:21 pm               SG99      Centromere drive and suppression by parallel pathways for recruiting microtubule destabilizers . T. Kumon, J. Ma, D. Stefanik, E. C. Nordgren, R. B. Akins, J. Kim, M. Levine, M. A. Lampson;  University of Pennsylvania, Philadelphia, PA.

4:33 pm               SG102    Cell cycle regulation of CENP-T in fission yeast. Q. Dong, F. Li;  Department of Biology, New York University, New York, NY.

Dynamic Modes of Cellular Compartmentalization

Scientific Tracks: Cellular Dynamics: Compartmentalization, Trafficking, Cytoskeleton, Division, and Migration, and Signaling and Metabolism: Integrating Intra- and Intercellular Signaling, and Information Processing

Organizers: Aidan Fenix, University of Washington; and Rosalie Lawrence, University of California San Francisco

Cellular compartmentalization is an essential feature of all known life. How dynamic compartmentalization enables cellular gene expression and signaling is a core question, with implications both for illuminating basic biology and understanding disease mechanisms. Due to the advent of advanced microscopy techniques and increasingly multi-disciplinary approaches, we are in the midst of an exciting revolution revealing many diverse forms and functions of dynamic cellular compartmentalization across size scales. Both “classical” membrane-bound organelles, and – more recently – membrane-less compartments have been shown to enable highly regulated transcription, translation, and signaling events at the right place and the right time. In this Special Interest Subgroup, speakers will present talks providing new insights into dynamic mechanisms of cellular compartmentalization. Talks will cover a variety of cellular compartments and scales regulating transcription, translation, and signaling, ranging from sub-nuclear compartments to cytoplasmic ensembles. Priority will be given to work presenting unpublished data.

1:45 pm                              Introduction by Aidan Fenix

1:50 pm               SG103    Protein compartmentalization enables robustness of bacterial cell cycle under metabolic shifts. S. Saurabh, T. Chong, C. Bayas, P. Dahlberg, W. E. Moerner, L. Shapiro;  Stanford University, Stanford, CA.

2:05 pm               SG104    Protein clustering mediated by an internal RNA-Binding Region accelerates the CTCF target search mechanism. A. S. Hansen;  Biological Engineering, Massachusetts Inst Technology, Cambridge, MA.

2:20 pm               SG105    Heterochromatin organization and dynamics. S. Sanulli;  Stanford University, Palo Alto, CA.

2:35 pm               SG106    Cells tune nucleolar phase behavior in response to developmental and environmental change. P. A, M. Couture, J. Goldberg, S. Biedzinski, S. C. Weber;  McGill University, Montreal, QC, CANADA.

2:50 pm               SG107    Differential regulation of mRNA transport during acute stress through phase separation of the nuclear mRNA export factor Nab2. S. Heinrich1, M. Hondele1, D. Marchand1, C. P. Derrer1, D. Grunwald2, K. Weis1;  1ETH Zurich, Zurich, SWITZERLAND, 2Medical School, University of Massachusetts, Worchester, MA.

3:05 pm               SG108    RNA aggregation in neurodegenerative disease. A. Jain;  Whitehead Institute, Cambridge, MA.

3:20 pm                              Break

3:35 pm               SG109    Differential Surface Charge on the Plasma Membrane Polarizes Cells During Migration. T. Banerjee1,2, D. S. Pal1, D. Biswas3, Y. Miao1,4, P. A. Iglesias3, P. N. Devreotes1;  1Department of Cell Biology and Center for Cell Dynamics, School of Medicine, Johns Hopkins University, Baltimore, MD, 2Department of Chemical and Biomolecular Engineering, Whiting School of Engineering, Johns Hopkins University, Baltimore, MD, 3Department of Electrical and Computer Engineering, Whiting School of Engineering, Johns Hopkins University, Baltimore, MD, 4Department of Biological Chemistry, School of Medicine, Johns Hopkins University, Baltimore, MD.

3:50 pm               SG110    Degradative tubular lysosomes link pexophagy to starvation and early aging in C. elegans. D. Dolese, M. Junot, B. Ghosh, T. Butsch, A. Johnson, A. Bohnert;  LSU, Baton Rouge, LA.

4:05 pm               SG111    In vivo investigation of organelle dynamics in the liver using a novel fluorescent multi-organelle transgenic mouse model. D. Feliciano1, S. Cohen2, N. Porat-Shliom1;  1NCI/NIH, Bethesda, MD, 2UNC, Chapel Hill, NC.

4:20 pm               SG112    Motion of single molecular tethers reveals dynamic subdomains at organelle contact sites. C. J. Obara1, J. Nixon-Abell2, F. Riccio2, C. Blackstone3, J. Lippincott-Schwartz1;  1HHMI Janelia Research Campus, Ashburn, VA, 2HHMI/NINDS, Ashburn, VA, 3NINDS/NIH, Bethesda, MD.

4:40 pm                              Break

How Oncogenes Rewire the Cell

Scientific Tracks: Cells in Distress and Disease: Cancer, Aging, Infection, Stress, Chemical Biology, and Therapeutics, and Signaling and Metabolism: Integrating Intra- and Intercellular Signaling, and Information Processing

Organizers: Cosimo Commisso, SBP Medical Discovery Institute; and Elda Grabocka, Thomas Jefferson University

Cellular stress comes in many forms and is an inherent feature of all tumors. Oncogenic mutations are known to rewire the tumorigenic landscape to facilitate adaptation to cellular stress and shape tumor progression. Such adaptive mechanisms include reprogramming of signaling and gene expression pathways, altered or enhanced biogenesis and function of specific organelles, and intercommunication with the tumor microenvironment. This special interest subgroup will focus on how oncogenes rewire the cell to circumvent stresses and promote carcinogenesis.

1:45 pm                              Introduction

1:50 pm               SG113    How BRCA mutations rewire stromal cells in the tumor microenvironment. G. Friedman1, L. Shaashua1, D. Kelsen2, I. Amit1, R. Scherz-Shouval1;  1Weizmann Institute of Science, Rehovot, ISRAEL, 2Memorial Sloan Kettering Cancer Center, New York, NY.

2:10 pm               SG114    Oncogene induced senescence in hematopoietic progenitors features myeloid restricted hematopoiesis and chronic inflammation. R. Biavasco, E. Lettera, E. Montini, R. Di Micco;  San Raffaele Hospital, San Raffaele Telethon Institute for Gene Therapy, Milan, ITALY.

2:30 pm               SG115    Migratory Transitions and Oncogenic Transformation in Epithelial Cells are Controlled by the Threshold of the Ras/PI3K/ERK Excitable Network. H. Zhan1, S. Bhattacharya2, H. Cai3, P. Iglesias2, C. Huang4, P. N. Devreotes1;  1Department of Cell Biology, Johns Hopkins University School of Medicine, Baltimore, MD, 2Department of Electrical and Computer Engineering, Johns Hopkins University, Baltimore, MD, 3Chinese Academy of Sciences Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, CHINA, 4Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD.

2:45 pm               SG116    Stress-adaptation in cancer: role of stress granules in KRAS-driven tumorigenesis. E. Grabocka;  Cancer Biology, Thomas Jefferson University, Philadelphia, PA.

3:05 pm                              Break

3:30 pm               SG117    Metabolic rewiring by NRF2 in cancer. G. M. DeNicola;  Cancer Physiology, Moffitt Cancer Center, Tampa, FL.

3:50 pm               SG118    The role of antioxidant enzyme GSTA2 in the nucleolus of mutant fallopian tube epithelial cells. R. Sowamber, I. Paudel, L. Dodds, O. Nelson, M. Castillo, L. Diaz, M. Schlumbrecht, S. George;  University of Miami Miller School of Medicine, Miami, FL.

4:05 pm               SG119    The Interplay Between Amino Acid Metabolism and Ferroptosis Sensitivity. S. Dixon;  Stanford University, Stanford, CA.

4:25 pm               SG120    The Macropinosome: An Oncogene-driven Organelle that Supports Metabolic Stress Tolerance. C. Commisso;  SBP Medical Discovery Institute, La Jolla, CA.

Mechanisms and Regulation of Membrane Trafficking

Scientific Track: Cellular Dynamics: Compartmentalization, Trafficking, Cytoskeleton, Division, and Migration

Organizers: Steven Caplan, University of Nebraska; Lei Lu, Nanyang Technological University; Rytis Prekeris, University of Colorado Anschutz Medical Campus; Nava Segev, University of Illinois at Chicago; and Elizabeth Sztul, University of Alabama, Birmingham

The membrane trafficking within a eukaryote primarily comprises the secretory, endocytic and autophagic pathways, which are tightly integrated with many other cellular functions. This session has three parts and is focused on three important aspects of the membrane trafficking respectively: the Golgi complex, endocytic trafficking and Rab and ARF/ARL GTPases. In the secretory pathway, the Golgi complex receives newly synthesized cargos from the endoplasmic reticulum and delivers them to the plasma membrane. Despite decades of research, the fundamental cellular mechanism by which the Golgi complex functions remains elusive and has been under constant debate. New insights into this organelle from recent advances in imaging technologies will be presented in the first part. Once cargos are transported to the plasma membrane, they are further subject to the endocytic trafficking for recycling or turnover. Studies have determined that specialized subsets of endosomes play key roles in regulating cytoskeletal dynamics and cellular functions such as cell division, cell migration, centrosome function and cilia formation, as well as cell polarization. The second part of this session features recent progress in this field. In the secretory, endocytic and autophagic pathways, small GTPases that belong to Rab and ARF/ARL families have been established as important regulators of almost every step of membrane trafficking. The third part of this session will explore mechanisms by which these small GTPases are themselves regulated and how, when activated, they coordinate multiple cellular processes.

1:45 pm                              Introduction 1

1:49 pm               SG121    Golgi and next-door neighbors - a comparative view of yeast, plant and animal cells. A. Nakano;  RIKEN Center for Advanced Photonics, Wako, JAPAN.

2:03 pm               SG122    The Molecular Organization and Cisternal Transport of the Golgi under the Light Microscopy. H. Tie, L. Lu;  Nanyang Technological University, Singapore, SINGAPORE.

2:17 pm               SG123    ER-to-Golgi protein delivery through an interwoven, tubular network extending from ER. C. Chang1, A. Weigel1, G. Shtengel1, C. Xu1, D. Hoffman2, M. Freeman1, N. Iyer1, W. Qui1, H. Hess1, J. Lippincott-Schwartz1;  1HHMI Janelia Research Campus, Ashburn, VA, 210X Genomics Inc, pleasanton, CA.

2:31 pm               SG124    Deciphering the Golgi logic circuit. J. C. Casler, N. Johnson, A. Pantazopoulou, A. H. Krahn, B. S. Glick;  Molecular Genetics and Cell Biology, University of Chicago, Chicago, IL.

2:45 pm                              Introduction 2

2:49 pm               SG125    The relationship between Rab11-endosomes and the centrosome during embryonic cell divisions. H. Hehnly, N. Krishnan, P. Fioramonti;  Syracuse University, Syracuse, NY.

3:03 pm               SG126    ApoER2, the Reelin Receptor, is a Cargo for the Adaptor Protein AP-4: Implications for Exocytic Trafficking and Polarized Sorting of ApoER2 in Neurons. M. Caracci1, P. Farfán1, I. Jausoro1, M. Bisbal2, R. De Pace3, J. S. Bonifacino3, G. Mardones4, M. Marzolo1;  1Departamento de Biología Celular y Molecular, Facultad de Ciencias Biológicas, Pontificia Universidad Catolica Chile, SANTIAGO, CHILE, 2Instituto Mercedes y Martin Ferreyra, Cordoba, ARGENTINA, 3Neurosci Cell Structural Biol Div, Eunice Kennedy Shriver National Institute of Child Health Hum Dev, National Institutes of Health, Bethesda, MD, 4Universidad Austral de Chile, Valdivia, CHILE.

3:17 pm               SG127    The Rab40 small GTPase subfamily regulates cell migration by modulating protein ubiquitylation. E. D. Duncan, E. Linklater, K. Han, R. Prekeris;  Cell and Developmental Biology, University of Colorado Denver Anschutz Medical Campus, Aurora, CO.

3:31 pm               SG128    The Retromer Complex Regulates Centriolar Satellite Recruitment and Ciliogenesis. S. Xie1, N. Naslavsky1, S. Caplan2;  1University of Nebraska Medical Ctr, Omaha, NE, 2University of Nebraska Medical Ctr, omaha, NE.

3:45 pm                              Introduction 3

3:49 pm               SG129    Calcium-stimulated disassembly of focal adhesions mediated by an ORP3/IQSec1 complex. J. E. Casanova1, R. S. D'Souza1, A. Turgut1, K. Servage2, K. Orth2, N. Sosale3, M. J. Lazzara3, J. C. Allegood4;  1Cell Biology, University of Virginia, Charlottesville, VA, 2Molecular Biology, HHMI, UT Southwestern, Dallas, TX, 3Chemical Engineering, University of Virginia, Charlottesville, VA, 4Biochemistry, Virginia Commonwealth University, Richmond, VA.

4:03 pm               SG130    Characterization of Rab5 isoforms in NGF signaling and cell differentiation. G. Li, W. Liu, Z. Liang;  Biochemistry and Molecular Biology, University of Oklahoma Hlth Sci Ctr, Oklahoma City, OK.

4:17 pm               SG131    GEFs regulation by membrane offers ways to modulate small GTPase signaling. A. Nawrotek1, M. Kryszke1, J. Cherfils1, M. Zeghouf2;  1Université Paris-Saclay, Gif-sur-Yvette, FRANCE, 2CNRS, Cachan, FRANCE.

4:31 pm               SG132    Rab33b regulates autophagy via a noncanonical rab binding protein. Y. Wu;  Umeå University, Umeå, SWEDEN.

The Physical Cell: Imaging and Manipulating

Scientific Track: Physical Cell: Bioengineering, Mechanobiology, and Synthetic Biology

Organizers: Maddy Parsons, Kings College London;  Tony Kanchanawong, NUS

Mechanical forces play a key role at macro and micro-scale levels in organism development, tissue homeostasis and disease. Given the intrinsic spatiotemporal nature of mechanical forces, imaging techniques are thereby essential for both the observation and perturbation of mechanobiological processes. Imaging mechano-chemical responses, from single molecules to whole tissues, represents and exciting and fundamentally important challenge to defining how forces drive dynamic biological function. This subgroup highlights the application of state-of-the-art microscopy techniques and tools, as well as emerging concepts and approaches to image the mechano-chemical signals that underpin cell behaviour across a range of scales, cells and organisms. We aim to bring biophysicists, cell, computation and chemical biologists together, seek synergy in ideas, and promote collaborations in this rapidly developing research field.

1:45 pm                              Introduction by Maddy Parsons and Tony Kanchanawong

1:47 pm               SG133    The multimodular lim domain protein testin recognizes stress fiber strain and contributes to cellular force generation. S. Sala, P. W. Oakes;  Loyola University Chicago, Maywood, IL.

2:07 pm               SG134    Integrin-dependent difference in force exertion correlates with actin cytoskeleton organization. M. Jo1, J. Li2, V. Jaumouillé3, C. M. Waterman3, T. A. Springer2, T. Ha1;  1Johns Hopkins University, Baltimore, MD, 2Harvard Medical School, Boston, MA, 3National Institutes of Health, Bethesda, MD.

2:15 pm               SG135    Cellular and molecular force regulation by receptor-mediated interactions. E. Cavalcanti-Adam;  Cellular Biophysics, Max Planck Institute for Medical Research, Heidelberg, GERMANY.

2:35 pm               SG136    Spatiotemporal control of talin-mediated mechanotransduction by inducible dimerization. S. F. H. Barnett, Y. Wang, M. Yu, S. Le, J. Yan, P. Kanchanawong;  National University of Singapore, Singapore, SINGAPORE.

2:43 pm               SG137    Mechanical forces and the nucleus: regulation of cell fate and integrity. S. A. Wickstrom;  Helsinki Institute of Life Science, University of Helsinki, Helsinki, FINLAND.

3:03 pm                              Break

3:29 pm               SG138    Measuring forces in 3D cellular structures: Mechanical regulation of epithelial cyst homeostasis and morphogenesis. D. E. Conway, V. Narayanan;  Virginia Commonwealth University, Richmond, VA.

3:49 pm               SG139    Chromatin Mechanics Dictates Subdiffusion and Coarsening Dynamics of Embedded Condensates. D. S. W. Lee, N. S. Wingreen, C. P. Brangwynne;  Princeton University, Princeton, NJ.

3:57 pm               SG140    Dissecting the intercellular forces shaping tissues. G. Charras1, J. Fouchard1, K. Yamamoto1, A. Proag2, M. Suzanne2, H. Turlier3, A. Kabla4;  1University College London, London, UNITED KINGDOM, 2Centre for Integrative Biology, Toulouse, FRANCE, 3College de France, Paris, FRANCE, 4Cambridge University, Cambridge, UNITED KINGDOM.

4:17 pm               SG141    Mechanical Compartmentalization of the Intestinal Organoid Enables Crypt Folding and Collective Cell Migration. C. Pérez-González1,2,3, G. Ceada1,2, F. Greco4, M. Matejcic1, M. Gómez-González1, N. Castro1, S. Kale4,5, A. Álvarez-Varela6, P. Roca-Cusachs1,2, E. Batlle6,7,8, D. Matic Vignjevic3, M. Arroyo4, X. Trepat1,2,8,9;  1Institute for Bioengineering of Catalonia (IBEC), Barcelona, SPAIN, 2Facultat de Medicina, Universitat de Barcelona, Barcelona, SPAIN, 3Insitut Curie, PSL Research University, CNRS UMR 144, Paris, FRANCE, 4LaCàN, Universitat Politècnica de Catalunya-BarcelonaTech, Barcelona, SPAIN, 5Mechanical Engineering Department, Virginia Tech, Blacksburg, VA, 6Institute for Research in Biomedicine (IRB Barcelona), Barcelona, SPAIN, 7Centro de Investigación Biomédica en Red de Cáncer (CIBERONC), Barcelona, SPAIN, 8Institució Catalana de Recerca i Estudis Avançats (ICREA), Barcelona, SPAIN, 9Centro de Investigación Biomédica en Red en Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Barcelona, SPAIN.

4:25 pm               SG142    Tissue dynamics during growth and repair. Y. Mao;  University College London, London, UNITED KINGDOM.

Wednesday, December 9, 1:45 pm to 4:45 pm

Covid-19 Infection: Deciphering Cellular Responses

Scientific Tracks: Cells in Distress and Disease: Cancer, Aging, Infection, Stress, Chemical Biology, and Therapeutics

Organizers: Sigrid Reinsch, NASA-Ames Res; and Olivia Steele-Mortimer, NIAID

The SARS-CoV-2 virus emerged in China in late 2019 and rapidly spread around the globe. Internationally the scientific community has responded at an unprecedented pace, sharing information and resources. The Coronavirus International Research Team (COV-IRT) is a community of scientists driving research into COVID-19, comprising more than 200 scientists from at least 69 institutions and 13 companies worldwide. COV-IRT projects include analyzing heterogeneity of the viral genome, host response to COVID-19 disease, potential co-infections, and identifying potential therapeutic targets for preclinical and clinical development. Speakers in this session include founding members of COV-IRT, as well as scientists from the broader ASCB community. Topics include viral diversity, entry, replication, and host responses both at the cellular and systemic level.

1:45 pm                              Introduction by Sigrid Reinsch

1:50 pm               SG143    Building a visual consensus model of the SARS-CoV-2 life cycle. J. Iwasa1, A. Liu1, J. Rogers2, M. Riggi1, M. Meyer2;  1Biochemistry, University of Utah, Salt Lake City, UT, 2Computer Science, University of Utah, Salt Lake City, UT.

2:00 pm               SG144    Length and flexibility enable the extended intermediate of the SARS-Cov-2 Spike protein to capture host cell membranes. R. Su, J. Zeng, S. Thiyagarajan, B. O'Shaughnessy;  Chemical engineering, Columbia University, New York, NY.

2:10 pm               SG145    The ACE2 SARS-CoV2 receptor and TMPRSS2/4 coreceptors localize to motile cilia of the respiratory tract and function during viral infections. P. K. Jackson1, I. T. Lee1, T. Nakayama2, C. Wu1, Y. Goltsev1, S. Jiang1, J. V. Nayak2, G. P. Nolan1;  1Baxter Laboratory for Stem Cell Biology, Stanford University School of Medicine, Stanford, CA, 2Otolaryngology, Stanford University School of Medicine, Stanford, CA.

2:30 pm               SG146    Spying on viruses: translation and replication dynamics of single RNA viruses. S. Boersma1, H. H. Rabouw1, L. J. M. Bruurs1, T. Pavlovič1, A. L. W. van Vliet2, F. J. M. van Kuppeveld2, M. E. Tanenbaum1;  1Oncode Institute, Hubrecht Institute–KNAW and University Medical Center Utrecht, Utrecht, NETHERLANDS, 2Virology Division, Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, NETHERLANDS.

2:50 pm                              Break

3:15 pm               SG147    Open and Collaborative Science in the Age of the Pandemic: COV-IRT, the COVID-19 International Research Team. A. Yousey1, T. Treangen2, A. Beheshti3;  1Children’s Hospital of Philadelphia, Philadelphia, PA, 2Rice University, Houston, TX, 3NASA Ames Research Center, Mountain View, CA.

3:30 pm               SG148    Hidden genomic diversity of SARS-CoV-2. T. Treangen;  Rice University, Houston, TX.

3:45 pm               SG149    The Bioenergetics of Covid-19. D. Wallace, J. Guarnieri;  Department of Pediatrics, Children s Hospital of Philadelphia, Philadelphia, PA.

4:05 pm               SG150    COVID-19 Related Gene Expression Varies According to Ancestry: Endocytosis, ROS, and Immunity.. E. S. Wurtele1, U. Singh2, K. S. Hernandez3;  1Genetics, Cell and Developmental Biology, Iowa State University, Ames, IA, 2Bioinformatics and Computational Biology, Iowa State University, Ames, IA, 3Section of Computational Biomedicine and Biomedical Data Science, University of Chicago, Chicago, IL.

4:25 pm               SG151  MERS host cell dependencies and the search for pan-coronavirus host factors. R. Broeckel, K. McNally, A. Chiramel, E. Chebishev, S. M. Best;
NIAID/NIH, Hamilton, MT.

Epithelial Stem Cells

Scientific Track: Communal Cell: Development, Differentiation, Regeneration, Stem Cells, Organs, and Organoids

Organizers: Kara McKinley, UC San Francisco; and Pantelis Rompolas, University of Pennsylvania

The diversity of cell types within epithelial tissues, as well as the mechanical and signaling crosstalk between these cells, present a rich suite of cell biological questions. These questions have become increasingly accessible in recent years through technological developments including improved live imaging strategies and ex vivo models such as organoids. This session builds on the first Epithelia and their Stem Cells subgroup in 2019, and brings a new suite of speakers to address exciting questions regarding the cell behaviors contributing to epithelial morphogenesis, homeostasis, and repair.

1:45 pm                              Introduction by Pantelis and Kara

1:50 pm               SG152    Defining the design principles and molecular mechanisms by which epithelial stem cells drive tissue expansion at single cell resolution. C. Blanpain;  UNIVERSITE LIBRE DE BRUXELLES, Brussels, BELGIUM.

2:10 pm               SG153    Corneal stem cell dynamics revealed by 2-photon live imaging. O. Farrelly, P. Rompolas;  Dermatology, Ophthalmology, Cell & Developmental Biology, University of Pennsylvania, Philadelphia, PA.

2:22 pm               SG154    Temporal and spatial dynamics of stem cells in lung regeneration at single cell resolution. P. Tata;  Department of Cell Biology, Duke University School of Medicine, Durham, NC.

2:34 pm               SG155    Neuroglian regulates Drosophila intestinal stem cell proliferation through enhanced signaling via the Epidermal Growth Factor Receptor. K. M. Cunningham1, M. Resnik-Docampo1, S. M. Ruvalcaba1, D. L. Jones1,2;  1Molecular, Cell, and Developmental Biology, University of California Los Angeles, Los Angeles, CA, 2Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California Los Angeles, Los Angeles, CA.

2:46 pm               SG156    The PAR polarity complex promotes epithelial integrity during intestinal morphogenesis. M. D. Sallee, M. Pickett, J. Feldman;  Biology, Stanford University, Stanford, CA.

2:58 pm                              Break

3:13 pm               SG157    Regulation of stem cell state reversibility by the niche. S. A. Wickström, L. C. Biggs, C. S. Kim;  Helsinki Institute of Life Science, University of Helsinki, Helsinki, FINLAND.

3:33 pm               SG158    Desmosomes Pattern Cell Mechanics to Govern Epidermal Tissue Form and Function. J. A. Broussard1, J. L. Koetsier2, K. J. Green1;  1Pathology and Dermatology, Northwestern University, Chicago, IL, 2Pathology, Northwestern University, Chicago, IL.

3:45 pm               SG159    Mechanochemical control of epidermal stem cell divisions by B-plexins. C. Jiang1,2, A. Javed3, L. Kaiser1, M. M. Nava3, D. Zhao1, D. T. Brandt1, J. F. Baldovinos1, L. Zhou1, C. Höß1, K. Sawmynaden4, A. Oleksy4, D. Matthews4, L. S. Weinstein5, H. J. Gröne1, C. M. Niessen6, S. Offermanns2,7, S. A. Wickström3,6,8,9,10, T. Worzfeld1,2;  1Institute of Pharmacology, University of Marburg, Marburg, GERMANY, 2Department of Pharmacology, Max-Planck-Institute for Heart and Lung Research, Bad Nauheim, GERMANY, 3Helsinki Institute of Life Science, Biomedicum Helsinki, University of Helsinki, Helsinki, FINLAND, 4LifeArc, Stevenage, UNITED KINGDOM, 5Metabolic Diseases Branch, NIDDK, NIH, Bethesda, MD, 6Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD), Center for Molecular Medicine Cologne, University of Cologne, Cologne, GERMANY, 7Medical Faculty, University of Frankfurt, Frankfurt, GERMANY, 8Wihuri Research Institute, Biomedicum Helsinki, University of Helsinki, Helsinki, FINLAND, 9Stem Cells and Metabolism Research Program, Faculty of Medicine, University of Helsinki, Helsinki, FINLAND, 10Max-Planck-Institute for Biology of Ageing, Cologne, GERMANY.

3:57 pm               SG160    The translational repressor Brat constrains regenerative growth to ensure proper patterning after tissue damage in Drosophila. S. N. F. Abidi, R. Smith-Bolton;  University of Illinois at Urbana-Champaign, Urbana, IL.

4:09 pm               SG161    Bimodal function of chromatin remodeler Hmga1 in neural crest induction and Wnt-dependent emigration. S. Gandhi, K. Maruszko, E. Hutchins, M. Bronner;  Biology and Biological Engineering, California Institute of Technology, Pasadena, CA.

4:21 pm               SG162    Cell sorting in Hydra vulgaris arises from differing capacities for epithelialization between cell types. T. D. Skokan1, R. D. Vale1,2, K. L. McKinley1;  1Cellular and Molecular Pharmacology; Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, CA, 2Howard Hughes Medical Institute, Janelia Research Campus, Ashburn, VA.

4:33 pm               SG163    From spikes to intercellular waves: tuning intercellular Ca2+ signaling dynamics modulates organ size control. D. Soundarrajan, F. Huizar, R. Paravitorghabeh, T. Robinett, J. Zartman;  University of Notre Dame, Notre Dame, IN.

Evolutionary Cell Biology

Scientific Track: Specialized Cell and Evolution: Neurobiology, Immunology, and Emerging Model Systems  

Organizers: Holly Goodson, University of Notre Dame; and Courtney Schroeder, Fred Hutchinson Cancer Research Center

Evolutionary cell biology (ECB) has two complementary aspects: One is using the perspectives and methods of evolutionary biology to gain insight into cell biological processes; the other is to use the biology and diversity of cells to gain insight into the process of evolution. These different perspectives are united by the fact that cells are the fundamental unit of life, and by the expectation that the study of ECB will both illuminate the diversity of life at (sub)cellular scales and help elucidate the fundamental principles of living systems. Because cells and cellular processes lie at the interface between chemistry, physics, and biology, biophysics and biochemistry have central roles in ECB. Speakers will address topics across the range of ECB, with possible examples including the use of patterns of protein evolution to dissect protein structure and function, the study of comparative cell biology to illuminate the characteristics of the last eukaryotic common ancestor, and the application of biophysics to elucidate the role of physical mechanisms in determining phenotype.

1:45 pm                              Introduction by Holly Goodson and Courtney Schroeder

1:50 pm               SG165    Rigorous strategies for inferring homology in eukaryotic organelles. S. C. Dawson1, L. Fritz-Laylin2;  1University of California, Davis, Davis, CA, 2University of Massachussetts Amherst, Amherst, MA.

2:05 pm               SG166    Choanoflagellate Cilia Structures Revealed by Cryo-Electron Tomography. J. Pinskey, A. Lagisetty, N. Phan, E. Reetz, L. Gui, D. Nicastro;  Cell Biology, UT Southwestern Medical Center, Dallas, TX.

2:20 pm               SG167    A rapidly evolving actin-related protein localizes to germline cytoskeletal structures for roles in Drosophila male fertility. C. M. Schroeder1, S. Tomlin1, J. R. Valenzuela1, H. S. Malik1,2;  1Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA, 2Howard Hughes Medical Institute, Seattle, WA.

2:35 pm               SG168    Conserved actin nucleators drive motility, phagocytosis, and osmoregulation in the evolutionarily divergent amoeba Naegleria. K. B. Velle, L. K. Fritz-Laylin;  University of Massachusetts, Amherst, Amherst, MA.

2:40 pm                              Break.

2:50 pm               SG169    The evolutionary diversity of eukaryotic cells, as seen with 2020 vision. A. Simpson;  Biology, Dalhousie University, Halifax, NS, CANADA.

3:05 pm               SG170    Evolutionary repair: Changes in multiple functional modules allow meiotic cohesin to support mitosis. Y. Hsieh1, V. Makrantoni2, D. Robertson2, A. L. Marston3, A. W. Murray1;  1Molecular and Cellular Biology, Harvard University, Cambridge, MA, 2The Wellcome Centre for Cell Biology,University of Edinburgh, Edinburgh, UNITED KINGDOM, 3The Wellcome Centre for Cell Biology, University of Edinburgh, Edinburgh, UNITED KINGDOM.

3:20 pm               SG171    Non-Mendelian chromosome segregation of selfish R2d2 locus in mouse oocytes. B. Clark1, E. Trimm2, M. A. Lampson2, T. Akera1;  1National Heart, Lung, and Blood Institute, NIH, Bethesda, MD, 2Department of Biology, University of Pennsylvania, Philadelphia, PA.

3:35 pm               SG172    Multiple mechanisms lead to chromosome segregation defects in inviable Xenopus hybrids. M. Kitaoka, R. Heald;  University of California, Berkeley, Berkeley, CA.

3:40 pm                              Break

3:50 pm               SG173    Immune factor of bacterial origin protects ticks against host skin microbes. B. Hayes1, A. Radkov1, F. Yarza1, S. Flores1, J. Kim1, Z. Zhao1, K. Lexa2, L. Marnin3, J. Biboy4, V. Bowcut1, W. Vollmer4, J. H. F. Pedra3, S. Chou1;  1Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA, 2Denali Therapeutics, San Francisco, CA, 3School of Medicine, University of Maryland, Baltimore, MD, 4Biosciences Institute, Newcastle University, Newcastle upon Tyne, UNITED KINGDOM.

4:05 pm               SG174    Hemichordate development at single-cell resolution and cell-type evolution of the chordate ancestor. J. Gray1, J. Briggs1, L. Peshkin1, J. Gerhart2, M. Kirschner1;  1Harvard Medical School, Boston, MA, 2University of California, Berkeley, Berkeley, CA.

4:20 pm               SG175    Mapping single-cell atlases across the animal tree of life unravels cell type evolution. A. J. Tarashansky1, J. M. Musser2, M. Khariton1, P. Li1, D. Arendt2,3, S. R. Quake1,4,5, B. Wang1,6;  1Department of Bioengineering, Stanford University, Stanford, CA, 2Developmental Biology Unit, European Molecular Biology Laboratory, Heidelberg, GERMANY, 3Centre for Organismal Studies, University of Heidelberg, Heidelberg, GERMANY, 4Chan Zuckerberg Biohub, San Francisco, CA, 5Department of Applied Physics, Stanford University, Stanford, CA, 6Department of Developmental Biology, Stanford University, Stanford, CA.

4:35 pm               SG176    Lineage dynamics of the endosymbiotic cell type in the soft coral Xenia. M. Hu, X. Zheng, C. Fan, Y. Zheng;  Carnegie Institution for Science, Baltimore, MD.

Frontiers in Cytokinesis

Scientific Tracks: Cellular Dynamics: Compartmentalization, Trafficking, Cytoskeleton, Division, and Migration

Organizers: Douglas Robinson, Johns Hopkins University Sch Med;  Julie Canman, Columbia University; Ulrike Eggert, King's College London; Amy Shaub Maddox, University of North Carolina; Dimitrios Vavylonis, Lehigh University; and Jian-Qiu, The Ohio State University

Cytokinesis is a spectacular cellular shape change, which requires coordination of complex cellular machinery over many scales of space and time. In animal cells, this includes signaling pathways that guide the rearrangement of spindle microtubules to position the division plane, assembly of a contractile actomyosin network at the division site, force production to drive a dramatic cell shape change, and timely remodeling of the plasma membrane. In a multicellular setting, cytokinesis further requires cell-cell and cell-environment communication. This geometrically simplified cell shape change serves as a paradigm for numerous other cell shape change events including those that take place during migration and tissue morphogenesis. In this 6th Biannual Special Interest Subgroup Meeting, we propose to bring together a group of investigators using systematic genetic and chemical methods, biophysical techniques for measuring contractility, high resolution imaging, diverse model organisms and cell types, and mathematical modeling. To ensure that our Subgroup meeting is exceptionally valuable to attendees, we will encourage our speakers to present newly-emerging results.

1:45 pm                              Introduction

1:48 pm               SG177    Bacterial division proteins in Bacillus subtilis display two distinct sets of dynamics with stationary FtsZ binding proteins required for the condensation of Z rings essential for cytokinesis. M. J. Holmes1, G. R. Squyres1, S. R. Barger2,3, B. R. Pennycook2,4,5, J. Ryan2,6, V. T. Yan2,7, E. C. Garner1;  1Molecular and Cellular Biology, Harvard University, Cambridge, MA, 2Physiology Course, Marine Biological Laboratory, Woods Hole, MA, 3Cell and Developmental Biology, SUNY Upstate Medical University, Syracuse, NY, 4Institute of Clinical Sciences, Faculty of Medicine, Imperial College London, London, UNITED KINGDOM, 5MRC London Institute of Medical Sciences, Imperial College London, London, UNITED KINGDOM, 6Department of Biology II and Center for Integrated Protein Science Munich (CIPSM), Human Biology and, Ludwig-Maximilians-Universität München, Planegg-Martinsried, GERMANY, 7Max Planck Institute for Cell Biology and Genetics, Dresden, GERMANY.

2:00 pm               SG178    Guiding the plant cytokinetic apparatus: essential motor and non-motor functions of phragmoplast orienting kinesin 2. P. Livanos1, A. Herrmann1,2, S. Müller1;  1ZMBP, University of Tübingen, Tübingen, GERMANY, 2University of Texas at Austin, Austin, TX.

2:12 pm               SG179    Identification of lipids bound to membrane-associated cytokinesis proteins. A. Paquola1,2, E. Storck2, C. Ozbalci2, S. J. Terry2, U. Eggert1,2;  1Department of Chemistry, SE1 1UL, King's College London, London, UNITED KINGDOM, 2Randall Centre for Cell and Molecular Biophysics, New Hunt’s House, Guy’s Campus, SE1 1UL, King's College London, London, UNITED KINGDOM.

2:24 pm               SG180    Actomyosin ring structure and its regulation by IQGAP during cytokinesis in yeast. K. Wang1, T. Svitkina2, E. Bi1;  1Department of Cell and Developmental Biology, University of Pennsylvania, Philadelphia, PA, 2Department of Biology, University of Pennsylvania, Philadelphia, PA.

2:36 pm                              Break

2:51 pm               SG181    Exploring the Functions of Two RNA-Binding Proteins within the Mechanoresponsive Network in Dictyostelium discoideum. Y. Liu, P. Kothari, D. N. Robinson;  Johns Hopkins University School of Medicine, Baltimore, MD.

3:03 pm               SG182    Ect2 and MPGAP drive the cortical excitability circuit. A. Michaud1, G. von Dassow2, M. Leda3, A. B. Goryachev3, A. Bolton1, W. M. Bement1;  1Center for Quantitative Cell Imaging, University of Wisconsin-Madison, Madison, WI, 2Oregon Institute for Marine Biology, University of Oregon, Charleston, OR, 3Centre for Synthetic and Systems Biology, University of Edinburgh, Edinburgh, UNITED KINGDOM.

3:15 pm               SG183    Writhing of cytokinetic contractile rings reveals that the contractile ring is an elastoporous cable. S. Thiyagarajan1, D. An1, R. Alonso-Matilla1, S. Wang1, T. G. Chew2, M. K. Balasubramanian2, B. O'Shaughnessy1;  1Columbia University, new york, NY, 2University of Warwick, Coventry, UNITED KINGDOM.

3:27 pm               SG184    Contractile ring component density dynamics influences contraction kinetics. D. Cortes1, M. DiSalvo2, N. Allbritton3, A. Maddox1;  1UNC Chapel Hill, Chapel Hill, NC, 2Johns Hopkins, Gaithersburg, MD, 3University of Washington, Seattle, WA.

3:39 pm                              Break

3:54 pm               SG185    Opposite Surfaces of the Cdc15 F-BAR Domain Create a Membrane Platform that Coordinates Cytoskeletal and Signaling Components for Cytokinesis. C. E. Snider, M. Chandra, N. A. McDonald, A. H. Willet, S. E. Collier, M. D. Ohi, L. P. Jackson, K. L. Gould;  Vanderbilt University, Nashville, TN.

4:06 pm               SG186    Cdc42 GTPase promotes timely Rho1 activation and septum ingression during cytokinesis. U. N. Onwubiko, A. Mitoubsi, E. Koory, M. Das;  University of Tennessee, Knoxville, TN.

4:18 pm               SG187    Ring canal formation in the Drosophila testis occurs via a midbody-like intermediate. K. Price, L. Cooley;  Yale University School of Medicine, New Haven, CT.

4:30 pm               SG188    A switch in the last step of cell division regulates the exit from naive pluripotency. A. Chaigne1, C. Labouesse2, I. J. White1, M. Agnew1, E. Hannezo3, K. J. Chalut2, E. K. Paluch4;  1MRC/LMCB, University College London, London, UNITED KINGDOM, 2University of Cambridge, Cambridge, UNITED KINGDOM, 3IST Austria, Vienna, AUSTRIA, 4Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge, UNITED KINGDOM.

Organelle Cross Talk and Contact Sites

Scientific Tracks: Signaling and Metabolism: Integrating Intra- and Intercellular Signaling, and Information Processing, and Cellular Dynamics: Compartmentalization, Trafficking, Cytoskeleton, Division

Organizers: Nuno Raimundo, Penn State College of Medicine; and Yvette Wong, Northwestern University

Cellular homeostasis is mediated by multiple organelles including mitochondria, lysosomes, endoplasmic reticulum, lipid droplets and peroxisomes. While the roles of individual organelles have been studied for decades, it is become increasingly appreciated that organelles functionally cross talk with one another, and further interact at inter-organelle membrane contact sites both in physiology and pathology. Importantly, organelle cross talk and contact sites are critical for regulating multiple signaling networks, and further have a role in diverse functions including metabolism, development and disease pathogenesis. This Special Interest Subgroup aims to bring together the researchers in the growing field of organelle cross talk and inter-organelle contact sites, to highlight the significant roles of these pathways in health and disease.

1:45 pm               SG189    Lipid droplet proteome dynamics and regulation. J. Olzmann;  Nutritional Sciences and Toxicology, University of California, Berkeley, Berkeley, CA.

2:05 pm               SG190    Mitochondria-lysosome contact sites in cellular homeostasis and neurodegenerative disease pathogenesis.. Y. C. Wong, S. Kim, W. Peng, D. Krainc;  Northwestern University Feinberg School of Medicine, Chicago, IL.

2:20 pm               SG191    VPS13 family proteins in the cross-talk between ER and mitochondria.A. Guillén-Samander1, M. Leonzino1, P. De Camilli2; 1Departments of Neuroscience and of Cell Biology, Yale University, New Haven, CT, 2Departments of Neuroscience and of Cell Biology, Yale University and HHMI, New Haven, CT

2:40 pm               SG192    Interplay mitochondria-lysosome regulates neuroinflammation in aging and disease. N. Raimundo; Penn State College of Medicine, Hershey, PA

2:55 pm               SG193    Lysosomal nucleotide metabolism regulates ER proteostasis via mTOR signaling. M. Wang;  Baylor College of Medicine/HHMI, Houston, TX.

3:15 pm                              Break

3:35 pm               SG194    Ribosome-Associated Vesicles: a dynamic sub-compartment of the endoplasmic reticulum in secretory cells. Z. Freyberg1, S. D. Carter2, C. M. Hampton3, R. Langlois4, R. Melero5, Z. J. Farino1, M. J. Calderon1, W. Li6, N. H. Tran7, R. A. Grassucci6, T. J. Morgenstern6, W. J. Rice8, Z. P. Wills1, S. Shiva1, F. Bartolini6, S. A. Murray1, M. Aridor1, K. N. Fish1, P. Walter7, D. Fass9, S. G. Wolf9, S. C. Watkins1, J. Carazo5, G. J. Jensen2, J. Frank6;  1University of Pittsburgh, Pittsburgh, PA, 2California Institute of Technology, Pasadena, CA, 3UES, Inc. at AFRL/RXAS, Wright-Patterson AFB, OH, 4Illumina, Inc., San Diego, CA, 5Centro Nacional de Biotecnología – CSIC, Madrid, SPAIN, 6Columbia University, New York, NY, 7University of California, San Francisco, San Francisco, CA, 8New York University, New York, NY, 9Weizmann Institute of Science, Rehovot, ISRAEL.

3:45 pm               SG195    Characterizing a novel tethering machinery enabling the contact site between mitochondria and the nucleus. M. Eisenberg-Bord1, N. Zung1, J. Collado2, L. Drwesh3, E. Fenech1, A. Fadel1, D. Rapaport3, R. Fernández-Busnadiego2, M. Schuldiner1;  1Weizmann Institute of Science, Rehovot, ISRAEL, 2Georg-August-Universität Göttingen, Göttingen, GERMANY, 3University of Tübingen, Tübingen, GERMANY.

3:55 pm               SG196    Loss of mitochondria-plasma membrane tethering adversely impacts organelle function and cellular fitness. A. J. White1, J. V. Dietz2, O. Khalimonchuk2, L. Lackner1;  1Northwestern University, Chicago, IL, 2University of Nebraska, Lincoln, NE.

4:05 pm               SG197    Mechanism of lipid droplet/mitochondria contacts and role of Perilipin 5 in lipid metabolism. G. Miner, C. So, S. Cohen;  University of North Carolina Chapel Hill, Chapel Hill, NC.

4:15 pm               SG198    Competition between distinct Pex30 complexes at multiple membrane contact sites regulate organelle homeostasis. J. Verissimo Ferreira, P. Carvalho;  Sir William Dunn School of Pathology, University of Oxford, Oxford, UNITED KINGDOM.

4:25 pm               SG199    Actin and INF2 at the intersection of organelle morphology and motility. C. R. Schiavon1, J. W. Feng2, O. A. Quintero2, G. S. Shadel1, U. Manor1;  1The Salk Institute for Biological Studies, La Jolla, CA, 2University of Richmond, Richmond, VA.

4:35 pm               SG200    Morphologically-discrete, ER subdomains support the translation of different types of mRNAs in response to ER-lysosome interactions. H. Choi1, Y. Liao1, Y. Yoon2, J. Grimm1, L. Lavis1, R. Singer1, J. Lippincott-Schwartz1;  1Janelia Research Campus, Ashburn, VA, 2Albert Einstein College of Medicine, Bronx, NY.

PTMs of the Cytoskeleton

Scientific Track: Cellular Dynamics: Compartmentalization, Trafficking, Cytoskeleton, Division, and Migration

Organizers: Riyad Seervai, Baylor College of Medicine;  Kristen Verhey, University of Michigan; and Anna Kashina, University of Pennsylvania

Chromatin remodelers, the “readers, writers, and erasers” of the epigenetic machinery, drive changes in gene expression through regulation of the well-established “histone code.” An associated, and often overlapping, set of machinery has been found to be responsible for regulating the cytoskeleton through post-translational modifications (PTM) of actin, tubulin, and their associated proteins. Recent work has begun to decipher how PTMs affect changes in protein conformation and/or lead to altered interactions with “reader” proteins of the cytoskeleton. Large-scale proteomic studies are beginning to uncover new PTMs which mark cytoskeletal proteins in a context- and isotype- specific manner. In this subgroup, we will compare and contrast the role of different PTMs in regulating cytoskeletal proteins, and explore how these marks form a complex language to regulate the cytoskeletal network within the cell.

1:45 pm                              Introduction by Riyad Seervai

1:50 pm               SG201    The Effect of Arginylation on Cellular Microtubules. B. MacTaggart, A. Kashina;  University of Pennsylvania, Philadelphia, PA.

2:05 pm               SG202    The tubulin code in microtubule dynamics regulation. A. Roll-Mecak;  National Institutes of Health, Bethesda, MD.

2:20 pm               SG203    Regulation of the formin protein INF2 by lysine-acetylated actin: effects on cytoplasmic actin polymerization and mitochondrial dynamics. M. A1,2, H. Higgs1;  1Biochemistry and Cell Biology, Geisel School of Medicine at Dartmouth, Hanover, NH, 2Dana Farber Cancer Institute, Harvard Medical School, Boston, MA.

2:35 pm                              Break

2:50 pm               SG204    Towards establishing a Rosetta Stone for theTubulin Code. T. Hotta, S. Haynes, M. Gebbie, A. Nesvizhskii, R. Ohi;  University of Michigan, Ann Arbor, MI.

3:05 pm               SG205    Post-translational regulation of the Actin Cytoskeleton. M. Balasubramanian;  University of Warwick, Coventry, UNITED KINGDOM.

3:20 pm               SG206    Set-ing methyl marks on the cytoskeleton. R. Seervai1, K. Verhey2, K. Rathmell3, R. Dere1, D. Tripathi1, I. Park1, C. L. Walker1;  1Baylor College of Medicine, Houston, TX, 2University of Michigan, Ann Arbor, MI, 3Vanderbilt University, Nashville, TN.

3:35 pm                              Break.

3:50 pm               SG207    UNC-45A is novel ATP-independent MT severing protein overexpressed in cancer and neurodegenerative diseases. J. Habicht1, A. Mooneyham1, A. Hoshino1, M. Shetty1, S. Lesne1, J. Troncoso2, M. Lee1, M. Gardner1, M. Bazzaro1;  1University of Minnesota, Minneapolis, MN, 2Johns Hopkins University, Johns Hopkins Medical School, MD.

3:51 pm               SG208    Spatiotemporal oxidation of L-plastin downmodulates actin-dependent cellular functions. E. Balta1, R. Hardt2, J. Liang1, H. Kirchgessner1, C. Orlik1, B. Jahraus1, S. Hillmer3, S. Meuer4, K. Hübner1, G. Wabnitz1, Y. Samstag1;  1Institute of Immunology-Section Molecular Immunology, Heidelberg University, GERMANY, 2Mass spectrometry Core facility, Center for Molecular Biology, Heidelberg University, GERMANY, 3Electron Microscopy Core Facility, Heidelberg University, GERMANY, 4Institute of Immunology, Heidelberg University, GERMANY.

3:52 pm               SG209    A novel mechanism that promotes mitotic spindle formation in human cells. E. Petsalaki1, S. Lilla2, N. Boutakoglou1, S. Zanivan2, G. Zachos1;  1Biology, University of Crete, Heraklion, GREECE, 2CRUK Beatson Institute, Glasgow, UNITED KINGDOM.

3:53 pm               SG210    A phospho-regulated signal motif determines subcellular localization of α-TAT1 for dynamic microtubule acetylation. A. Deb Roy1, E. G. Gross1, G. S. Pillai1, S. Seetharaman2, S. Etienne-Manneville2, T. Inoue1;  1Department of Cell Biology, Johns Hopkins Medical Institute, Baltimore, MD, 2Department of Cell Biology and Cancer, Institut Pasteur, Paris, FRANCE.

3:54 pm               SG211    Actin Redox Balance Regulates Filament Disassembly. H. Wioland1, J. Bai2, T. Advedissian2, S. Frémont2, A. Echard2, A. Jégou1, G. Romet-Lemonne1;  1Institut Jacques Monod, Université de Paris, CNRS, Paris, FRANCE, 2Institut Pasteur, CNRS, Paris, FRANCE.

3:55 pm               SG212    Microtubule Glutamylation Specifies Polarized Distribution of Endoplasmic Reticulum. P. Zheng1, C. Obara2, E. Szczesna1, J. Nixon-Abell2, K. Mahalingan1, A. Roll-Mecak1, J. Lippincott-Schwartz2, C. Blackstone1;  1NINDS, NIH, Bethesda, MD, 2Janelia Research Campus, HHMI, Ashburn, VA.

3:56 pm               SG213    Fyn-mediated phosphorylation of tau differentially regulates the transport of early endosomes and lysosomes. L. Balabanian1, D. V. Lessard2, P. Yaninska3, P. W. Wiseman4, C. L. Berger2, A. G. Hendricks1;  1Department of Bioengineering, McGill University, Montreal, QC, CANADA, 2Department of Molecular Physiology and Biophysics, University of Vermont, Burlington, VT, 3Department of Physics, McGill University, Montreal, QC, CANADA, 4Departments of Physics and Chemistry, McGill University, Montreal, QC, CANADA.

3:57 pm               SG214    The mechanism and impact of actin N-terminal acetylation. T. Arnesen1,2;  1Department of Biomedicine, University of Bergen, Bergen, NORWAY, 2Department of Surgery, Haukeland University Hospital, Bergen, NORWAY.

4:12 pm               SG215    Tubulin glycylation controls axonemal dyneins, mammalian sperm flagellar motility and fertility. S. Gadadhar1,2, G. Alvarez Viar3, J. N. Hansen4, A. Gong5, A. Kostarev3, C. Ialy-Radio6,7, S. Leboucher1,2, A. Ziyyat6,7, A. Touré6, L. Alvarez5, G. Pigino3, C. Janke1,2;  1Institut Curie, PSL Research University, Orsay, Paris, FRANCE, 2Cnrs umr 3348, Université Paris Sud, Universite Paris Saclay, Orsay, Paris, FRANCE, 3Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, GERMANY, 4Biophysical Imaging, University Hospital Bonn, University of Bonn, Institute of Innate Immunity, Bonn, GERMANY, 5Center of Advanced European Studies and Research, Bonn, GERMANY, 6INSERM U1016, CNRS UMR8104, Université de Paris, Institut Cochin, Paris, FRANCE, 7Service d’histologie, d’embryologie, Biologie de la Reproduction, AP-HP, Hôpital Cochin, Paris, FRANCE.

4:27 pm               SG216    N-terminal modification of actin by acetylation and arginylation determines the architecture and assembly rate of linear and branched actin networks. S. Jansen;  Cell Biology and Physiology, Washington University St. Louis, St. Louis, MO.

Reimagining Cell Biology Through Quantitative Tools

Scientific Tracks: Physical Cell: Bioengineering, Mechanobiology, and Synthetic Biology, and Cellular Genome: 4D Organization, Expression, Replication, and Repair

Organizers: Jim Galbraith, OHSU Spatial Systems Biology; Catherine Galbraith, OHSU; Stefan Hinz, City of Hope; Jianhua Xing, University of Pittsburgh; Denis Tsygankov, Georgia Tech; and Ashok Prasad, Colorado State University

In order to understand the molecular processes that enable life, we need to evolve our tools and methods. Advances in technologies allow us to analyze, integrate, and synergize biological data more closely – leading to new biological discoveries, and empowering us to ask questions that were previously inconceivable.  In this subgroup, we will explore how new technologies, computational methods, and mathematical models allow us to explore the frontiers of biological discovery by reimagining the boundaries of our thinking.

1:45 pm                              Introduction by Organizers

1:47 pm                              Introduction by Ashok Prasad, Jianhu Xing, and Denis Tsygankov.

1:52 pm               SG217    High-throughput cell phenotyping in cancer and aging. D. Wirtz, P. Wu, J. Philip;  Johns Hopkins University, Baltimore, MD.

2:11 pm               SG218    Defining states of pluripotency at the single cell level using statistical thermodynamics. A. L. Plant, M. Halter, J. Stinson, S. Sarkar, J. B. Hubbard;  Biosystems and Biomaterials, Natl Inst Standards & Technol, Gaithersburg, MD.

2:30 pm               SG219    Physical organization of the cytosol. A. Gladfelter;  Department of Biology, UNC Chapel Hill, Chapel Hill, NC.

2:49 pm                              Break

3:04 pm                              Introduction by Catherine Galbraith and Jim Galbraith.

3:09 pm               SG220    Cell states beyond transcriptomics: integrating structural organization and gene expression in hiPSC-derived cardiomyocytes. M. Hendershott, B. Zaunbrecher, K. Gerbin, T. Grancharova, R. Donovan-Maiye, R. Gunawardane, J. Theriot;  Allen Institute for Cell Science, Seattle, WA.

3:28 pm               SG221    Quantification of cancer cell morphology and signaling. R. Fiolka;  University of Texas Southwestern Medical Center, Dallas, TX.

3:47 pm               SG222    Break

4:02 pm                              Introduction by Stefan Hinz

4:07 pm               SG223    Defining cell based biomarkers of ageing. J. M. Phillip;  Biomedical Engineering, Johns Hopkins University, Baltimore, NY.

4:26 pm               SG224    4D Cell Biology. J. Schöneberg;  UC San Diego, San Diego, CA.

Friday, December 11, 1:45 pm to 4:45 pm

Bacterial and Archaeal Cell Organization

Scientific Tracks: Signaling and Metabolism: Integrating Intra- and Intercellular Signaling, and Information Processing, and Specialized Cell and Evolution: Neurobiology, Immunology, and Emerging Model Systems

Organizers: Saumya Saurabh, Stanford University;  Courtney Ellison, Princeton University; and Georgia Squyres, Harvard University

Despite their small size and lack of traditional membrane-bound organelles, bacteria are not mere “bags of enzymes.” Over the past two decades, advances in light and electron microscopy have revealed a diverse array of subcellular structures that bacteria use to organize molecules in both space and time. For example, bacterial microcompartments sequester metabolic enzymes inside polyhedral protein shells, and storage granules accumulate excess nutrients into spherical aggregates of long-chain polymers. In addition to these discrete organelle-like structures, chromosomal loci and RNA transcripts are positioned at specific subcellular locations. Bacteria also contain cytoskeletal filaments, biomolecular condensates, and motor-driven, dynamic nanomachines that span from the subcellular space to the extracellular environment. This subgroup will highlight recent progress in identifying the mechanisms by which bacteria establish and dynamically regulate intracellular organization, as well as highlight new research on multicellular interactions between bacteria. Talks will also feature new developments in quantitative imaging, synthetic biology and biophysical modeling, which promise to uncover how cell organization impacts cell function. This will be the second iteration of an exciting special interest subgroup focusing on the intricacy of subcellular organization in single-cell organisms.

1:45 pm               SG225    A Multiscale Analysis of Bacterial Predation. T. Mignot;  CNRS-Aix Marseille University, Marseille, FRANCE.

1:59 pm               SG226    High-Resolution Imaging of Single-Cell Behaviors in 3D Bacterial Biofilms using Lattice-Light Sheet Microscopy and Machine Learning-Based Image Processing. M. Zhang1, J. Zhang1, Y. Wang1, J. Wang2, A. M. Achimovich3, S. T. Acton2, A. Gahlmann1;  1Chemistry, University of Virginia, CHARLOTTESVILLE, VA, 2Electrical and Computer Engineering, University of Virginia, CHARLOTTESVILLE, VA, 3Molecular Physiology & Biological Physics, University of Virginia, CHARLOTTESVILLE, VA.

2:13 pm               SG227    The Type IV Pilus Motor - Mechanism and Dynamics. L. L. Burrows;  McMaster University, Hamilton, ON, CANADA.

2:27 pm               SG228    Studying horizontal gene transfer in bacterial systems using a cell biological approach. A. Dalia;  Indiana University, Bloomington, IN.

2:41 pm                              Break 1

2:54 pm               SG229    Trigger mechanism for contraction of the Type VI secretion system sheath.. M. Basler;  Biozentrum, University of Basel, Basel, SWITZERLAND.

3:08 pm               SG230    Cytoskeleton Dynamics and Compartmentalized Growth in Archaea. A. Bisson;  Brandeis University, Waltham, MA.

3:22 pm               SG231    SepF is the FtsZ anchor in Archaea: implications for cell division in the Last Universal Common Ancestor (LUCA). N. Pende1, A. SOGUES CASTREJON1, D. MEGRIAN NUNEZ1, H. Palabikyan2, A. SARTORI-RUPP1, M. Graña3, S. K. Rittmann2, A. Wehenkel1, P. M. Alzari1, S. Gribaldo1;  1Institut Pasteur, Paris, FRANCE, 2University of Vienna, Vienna, AUSTRIA, 3Institut Pasteur, Montevideo, URUGUAY.

3:36 pm               SG232    Characterizing actin homologs in halophilic archaea. J. Zheng1, V. Hale2, M. Pohlschröder3, D. Safer4, J. Löwe2, A. Bisson-Filho5, E. Garner1;  1Molecular Cellular Biology Department, Harvard University, Cambridge, MA, 2MRC Laboratory of Molecular Biology, Cambridge Biomedical Campus, Cambridge, UNITED KINGDOM, 3Department of Biology, University of Pennsylvania, Philadelphia, PA, 4Department of Physiology, University of Pennsylvania, Philadelphia, PA, 5Department of Biology, Brandeis University, Waltham, MA.

3:50 pm                              Break 2

4:03 pm               SG233    No membrane, no problem: condensing bacterial organelles. B. S. Parmar, S. Biedzinski, J. Wall, S. C. Weber;  McGill University, Montreal, QC, CANADA.

4:17 pm               SG234    Cryogenic correlative single-molecule fluorescence localizations and electron tomography reveals bacterial subcellular organization. P. Dahlberg, S. Saurabh, A. Sartor, J. Wang, P. Mitchell, L. Shapiro, W. Chiu, W. Moerner;  Chemistry, Stanford University, Stanford, CA.

4:31 pm               SG235    Unraveling the molecular basis of chromosome organization using single-molecule and genomic scale technologies. M. Guo1, R. Kawamura2, M. Littlehale1, J. Marko2, M. Laub1;  1Biology, Massachusetts Institute of Technology, Cambridge, MA, 2Molecular Biosciences, Northwestern University, Evanston, IL.

Bioengineering in vitro Models of Disease

Scientific Tracks: Physical Cell: Bioengineering, Mechanobiology, and Synthetic Biology, and Cells in Distress and Disease: Cancer, Aging, Infection, Stress, Chemical Biology, and Therapeutics

Organizers: David Mack, University of Washington and Nicholas Geisse, Curi Bio

Techniques borrowed from the world of engineering have given us unprecedented insight into some of the fundamental underlying principles of cell biology. In recent years, these approaches have been used to generate new models of human disease for in vitro studies that would simply not be possible with less complex model systems. For example, human skeletal and cardiac muscle function is highly reliant on the complex interplay between cells and their extracellular environment. Nanofabrication techniques can be used to reproduce the extracellular disease phenotype, while stem cell engineering technologies enable the creation of healthy and diseased phenotypes side-by-side. In this Special Interest Subgroup, we will explore contemporary uses of these approaches, and how they have driven the development of novel insights into human disease, and how they have set the stage for the development of novel therapies that do not rely on the use of animal or human models.

1:45 pm                              Introduction by Nicholas Geisse

1:47 pm               SG236    Lessons Learned Modeling Human Diseases In Vitro. K. Parker;  Harvard University, Cambridge, MA.

2:02 pm               SG237    Human Engineered Heart Tissues as a Model of Duchenne Muscular Dystrophy. D. L. Mack1, S. Bremner2, N. Sniadecki3;  1Rehabilitation Medicine, University of Washington, Seattle, WA, 2Bioengineering, University of Washington, Seattle, WA, 3Mechanical Engineering, University of Washington, Seattle, WA.

2:17 pm               SG238    Generation of a rat model for EDMD lacking the emerin gene. G. Valdez;  Brown University, Providence, RI.

2:32 pm               SG239    In vitro and in vivo rescue of alpha-dystroglycan glycosylation upon gene editing of FKRP mutant iPS cells using a universal approach. R. Perlingeiro1, N. Dhoke1, H. Kim1, S. Selvaraj1, K. Azzag1, C. Ortiz-Cordero1, Q. L. Lu2, A. Bang3;  1Department of Medicine, Lillehei Heart Institute, University of Minnesota, Minne, Minneapolis, MN, 23McColl-Lockwood Laboratory for Muscular Dystrophy Research, Cannon Research Center, Carolinas Medical Center, Atrium Health, Charlotte, NC, 3Sanford Burnham Prebys Medical Discovery Institute, La Jolla, CA.

2:47 pm                              Break & Networking Session 1

3:02 pm               SG240    Mechanobiology of human induced pluripotent stem cell derived cardiomyocyte models. C. Blair1, O. Chirikian1, E. Castillo1, S. Feinstein1, K. Lane1, G. Pardon2, A. Schroer2, B. Pruitt1;  1UCSB, Santa Barbara, CA, 2Stanford, Palo Alto, CA.

3:17 pm               SG241    Post-Infarction Inflammation Increases Matrix Production in iPSC-Derived Cardiac Fibroblasts via NF-κB. A. J. Whitehead, A. J. Engler;  Bioengineering, University of California, San Diego, La Jolla, CA.

3:32 pm               SG243    Engineering Microscale Models of Cardiac and Skeletal Muscle Tissues to Probe Cell-Cell Interactions in Health and Disease. M. L. McCain;  Biomedical Engineering, University of Southern California, Los Angeles, CA.

3:47 pm               SG242    Structured-surface cultureware promotes myotube alignment and improves in vitro assay robustness. N. Estrella;  Rare Disease Research Unit, Pfizer, Inc, Cambridge, MA.

4:02 pm                              Break & Networking Session 2

4:15 pm               SG244    3D engineered heart tissues meet 3D chromatin organization: in vitro modelling of nuclear pathology in dilated cardiomyopathy. A. Bertero1, P. A. Fields1, A. S. T. Smith2, A. Leonard3, K. Beussman3, N. J. Sniadecki3, D. Kim4, L. Pabon1, J. Shendure5, W. S. Noble5, C. E. Murry1;  1Department of Laboratory Medicine & Pathology, University of Washington, Seattle, WA, 2Department of Physiology & Biophysics, University of Washington, Seattle, WA, 3Department of Mechanical Engineering, University of Washington, Seattle, WA, 4Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, 5Department of Genome Sciences, University of Washington, Seattle, WA.

4:30 pm               SG245    DNA-directed patterning enables fabrication of an in vitro bone marrow niche to study prostate tumor cell dormancy. M. Kozminsky, L. L. Sohn;  University of California, Berkeley, Berkeley, CA.

Biological Size Control and Scaling

Scientific Tracks: Physical Cell: Bioengineering, Mechanobiology, and Synthetic Biology, and Signaling and Metabolism: Integrating Intra- and Intercellular Signaling, and Information Processing

Organizers: Matt Good, University of Pennsylvania and Jan Skotheim, Stanford University;  

Proliferating eukaryotic cells coordinate growth, division, and differentiation processes. A broad variety of cell types, from yeast to human, contain a size checkpoint - a mechanism ensuring that they achieve a defined amount of growth or achieve a specific size in order to trigger the cell division cycle or differentiation. Recent studies have begun to uncover the biosynthetic basis of cell size regulation providing evidence to support adder and sizer models of growth control both in cultured cells in vitro and in stem cells in vivo. Additionally, cells undergo transient alterations of cell volume during mitosis, upon cell spreading on substrate and in specific stress conditions. An emerging phenomenon is that perturbations to cell size are sufficient to alter cell physiology, mechanics, subcellular scaling and gene expression, and that evolution has leveraged changes in cell size to regulate events in the context of tissue biology and early embryo development. This session will feature cutting-edge research on how cells and tissues sense and control their sizes and how cell size variation contributes to cellular decision-making. We will highlight new concepts and techniques used to characterize cell size and growth regulation, as well as survey the latest insights on the mechanistic basis of cell size control. Additional topics may include the interdependence of cell growth, biosynthesis, and cell division that maintains protein, RNA, and organelle homeostasis. Moreover, topics for discussion will include dysregulation of cell size in the context of disease and the size invariant scaling of signaling gradients in tissues and embryos.

1:45 pm                              Introduction

1:47 pm               SG246    Cortical Pattern Formation and Cell Sizes. M. Wu;  Yale University, New Haven, CT.

2:02 pm               SG247    Mass measurements during lymphocytic leukemia cell polyploidization decouple cell cycle and cell size dependent growth. L. Mu1, J. Kang1, S. Olcum1, K. R. Payer2, N. L. Calistri1, R. J. Kimmerling1, S. R. Manalis1, T. P. Miettinen1;  1Koch Institute, Massachusetts Institute of Technology, Cambridge, MA, 2Massachusetts Institute of Technology, Cambridge, MA.

2:14 pm               SG248    Scaling of Gene Expression with Cell Size May Explain Size Control in Yeast. Y. Chen1, G. Zhao1, J. Zahumensky2, S. Honey1, B. Futcher1;  1Microbiology and Immunology, Stony Brook University, Stony Brook, NY, 2Dept. of Functional Organization of Biomembranes, Institue of Experimental Medicine of the Czech Academy of Sciences, Prague, CZECH REPUBLIC.

2:29 pm               SG249    The coordination of biosynthetic scaling with cell size and growth. M. Swaffer1, G. Marinov1, H. Zheng2, A. Jones3, B. Snijders3, W. Greenleaf1, R. Reyes2, J. Skotheim1;  1Stanford University, Stanford, CA, 2McGill University, Montreal, QC, CANADA, 3The Francis Crick Institute, London, UNITED KINGDOM.

2:42 pm               SG250    The role of osmotic forces in nuclear size control. J. LEMIERE1, T. Fai2, P. Real Calderon1, F. Chang1;  1Department of Cell & Tissue Biology, UCSF, San Francisco, CA, 2Mathematics, Brandeis University, Waltham, MA.

2:55 pm               SG251    Coordination of Mitochondrial Homeostasis with Cell Size. K. M. Schmoller, A. Seel, F. Padovani;  Helmholtz Zentrum München, München, GERMANY.

3:10 pm                              Break

3:25 pm               SG252    Cell size is a determinant of stem cell potential during aging. J. Lengefeld1, C. Cheng1, P. Maretich1, M. Blair2, H. Hagen1, M. R. McReynolds3, E. Sullivan1, K. Majors1, C. Roberts4, J. Kang1, J. D. Steiner4, T. P. Miettinen1, S. R. Manalis1, A. Antebi4, S. J. Morrison5, J. A. Lees1, L. A. Boyer2, Ö. H. Yilmaz1, A. Amon1;  1Koch Institute For Integrative Cancer Research, MIT, Cambridge, MA, 2Department of Biology, MIT, Cambridge, MA, 3Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ, 4Max Planck Institute for Biology of Ageing and CECAD, Cologne, GERMANY, 5Children’s Research Institute and Department of Pediatrics, University of Texas Southwestern Medical Center, Dallas, TX.

3:38 pm               SG253    On Mechanisms How Cell Size Regulates Zygotic Genome Activation. H. Chen1, M. Good1,2;  1Department of Cell and Developmental Biology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, 2Department of Bioengineering, School of Engineering and Applied Science, University of Pennsylvania, Philadelphia, PA.

3:51 pm               SG254    Nuclear to cytoplasmic ratio control of cell size in developing Drosophila embryos. Y. Shindo1,2, S. Syed3, H. Wilky2, B. Lim3, A. A. Amodeo1,2;  1Biological Sciences, Dartmouth College, Hanover, NH, 2Princeton University, Princeton, NJ, 3University of Pennsylvania, Philadelphia, PA.

4:06 pm               SG255    Investigation of cellular dry mass density regulation in growth plate chondrocytes. S. Oh, C. Lee, C. Tabin, M. Kirschner;  Harvard Medical School, Boston, MA.

4:19 pm               SG256    Control of osteoblast regeneration by a train of Erk activity waves. A. De Simone, M. N. Evanitsky, L. Hayden, B. D. Cox, J. Wang, V. A. Tornini, J. Ou, A. Chao, K. D. Poss, S. Di Talia;  Duke University Medical Center, Durham, NC.

4:32 pm               SG257    Cell-to-organism scaling relationships in Xenopus embryos. C. Cadart, C. Erikson, K. Miller, R. Heald;  Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA.


Cell-matrix and Cell-Cell Interactions in 3D Environments

Scientific Tracks: Physical Cell: Bioengineering, Mechanobiology, and Synthetic Biology, and Cellular Dynamics: Compartmentalization, Trafficking, Cytoskeleton, Division, and Migration

Organizers: Bojana Gligorijevic, Temple University;  Ryan Petrie, Drexel University; Edna Cukierman, Fox Chase Cancer Center; and Matthew Kutys, University of California, San Francisco

Breakthroughs in imaging technologies, microphysiological systems, engineered ECMs, synthetic biology, and cell mechanobiology have revolutionized our ability to precisely define cell-ECM interactions, as well as assemble complex 3D human tissues. These combined advances have provided mechanistic insight into how chemomechanical signaling at cell-ECM and cell-cell adhesions in 3D and in vivo regulates cell fate and behavior to ultimately govern the multiscale coordination of single and multicellular morphogenic processes. As a result, recent discoveries have illuminated key regulatory mechanisms operating at the cell-cell and cell-ECM interfaces that have previously evaded developmental, cell, immune, and cancer biologists. This special interest subgroup will present the most recent advances in our understanding of how fundamental chemical and physical mechanisms at cell-cell and cell-ECM interfaces operate across biological scales to regulate diverse 3D cellular and tissue morphogenic behaviors.

1:45 pm                              Introduction by Matthew Kutys

1:48 pm               SG258    Matrix mechanics and dynamics during branching morphogenesis. C. M. Nelson;  Princeton University, Princeton, NJ.

2:07 pm               SG259    EPH/EPHRIN regulates organization by cortical actomyosin contractility effects on cell contacts and interfacial tension. A. A. Kindberg, V. Srivastava, J. M. Muncie, V. M. Weaver, Z. J. Gartner, J. O. Bush;  University of California- San Francisco, San Francisco, CA.

2:19 pm               SG260    Cadherin-mediated force transduction underlying epithelial barrier regulation during mitosis. J. L. Monster1, L. Donker1, M. J. Vliem1, Z. Win2, H. K. Matthews2, J. de Rooij1, B. Baum2, M. Gloerich1;  1UMC Utrecht, UMC Utrecht, NETHERLANDS, 2MRC-LMCB, UCL, London, UNITED KINGDOM.

2:38 pm               SG261    Unravelling the Mechanobiology of Lymph Node ECM: Implications how swelling might impact Il-7 mediated homeostasis. V. Vogel;  ETH Zurich, Zurich, SWITZERLAND.

2:57 pm                              Break 1

3:12 pm                              Introduction by Bojana Gligorijevic.

3:15 pm               SG262    Mechanical mapping of tumor cell extravasation in vivo. K. Tanner;  NCI/NIH, Bethesda, MD.

3:34 pm               SG263    Mechanics and mechanisms of 3D mesenchymal cell migration. A. D. Doyle1, D. Sykora1, G. Pacheco1, M. Kutys2, K. M. Yamada1;  1National Institute of Dental and Craniofacial Research, NIH, Bethesda, MD, 2Biomedical Sciences, University of California San Francisco, San Francisco, CA.

3:46 pm               SG264    Cell adhesion and mechanotransduction at filopodia tips. M. Miihkinen1, M. Grönloh1, J. Ivaska1, G. Jacquemet2;  1University of Turku, Turku, FINLAND, 2Åbo Akademi University, Turku, FINLAND.

3:58 pm               SG265    Age against the machine: How aging drives cancer progression. A. Weeraratna;  Johns Hopkins Schools of Public Health and Medicine, Baltimore, MD.

4:17 pm               SG266    The glutamatergic synapse protein NetG1 regulates pro-tumor functions of cancer associated fibroblasts in pancreatic cancer. R. Francescone1, D. Barbosa Vendramini-Costa1, J. Franco-Barraza1, J. Wagner1, A. Muir2, A. Lau3, L. Gabitova1, T. Pazina1, S. Gupta1, T. Luong1, N. Shah1, D. Rollins1, D. Restifo1, Y. Zhou1, Q. Cai1, Y. Tan1, W. Kruger1, A. Klein-Szanto1, H. Wang4, W. El-Deiry5, M. Vander Heiden3, S. Peri1, K. Campbell1, I. Astsaturov1, E. Cukierman1;  1Fox Chase Cancer Center, Philadelphia, PA, 2University of Chicago, Chicago, IL, 3Massachusetts Institute of Technology, Cambridge, MA, 4The University of Texas MD Anderson Cancer Center, Houston, TX, 5Brown University, Providence, RI.

4:29 pm                              Break 2

Centrioles, Basal Bodies, and Centrosomes

Scientific Tracks: Cellular Dynamics: Compartmentalization, Trafficking, Cytoskeleton, Division, and Migration, and Cells in Distress and Disease: Cancer, Aging, Infection, Stress, Chemical Biology, and Therapeutics

Organizers: Susan Dutcher, Washington University Sch Med; and Elif Nur Firat-Karalar, University of Koc

Centrioles are highly conserved microtubule-based structures that function as basal bodies to assemble cilia and recruit pericentriolar material to form centrosomes. They plan key roles during development and homeostasis. This proposed subgroup will address key questions and debates about the structure and functions of centrioles, basal bodies and centrosomes.We hope to add 2-3 additional talks from submitted abstracts.There have been multiple subgroups and minisymposia on cilia and microtubules, but not on centrioles per se. The speakers use a variety of approaches and different model organisms. We think these are key areas that will engender discussion and point to new directions in basic biology to human disease:

1. What are the mechanisms that control centriole number?
2. What is the molecular and functional relationship of centriolar satellites to centrosomes?
3. What are the mechanisms to ensure centriolar stability?
4. What are the developmental consequences of centriole abnormalities?
5. How do centrioles act with respect to aneuploidy and polyploidy?


1:45 pm                              Introduction by Susan Dutcher

1:48 pm               SG267    Revealing the molecular assembly of the centriole architecture using a combination of cryo-tomography and expansion microscopy.. V. HAMEL;  Cell Biology, University of Geneva, GENEVA, SWITZERLAND.

2:03 pm               SG268    Centrioles compartmentalize a permanent pool of building blocks to encode a temporal memory of their duplications.. M. G. Aydogan1, M. Mofatteh2, Z. Wilmott3, F. Zhou4, J. Bohlen5, J. W. Raff2;  1Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA, 2Sir William Dunn School of Pathology, University of Oxford, Oxford, UNITED KINGDOM, 3Mathematical Institute, University of Oxford, Oxford, UNITED KINGDOM, 4Ludwig Institute for Cancer Research, University of Oxford, Oxford, UNITED KINGDOM, 5German Cancer Research Center, Heidelberg, GERMANY.

2:18 pm               SG269    An Acentriolar Centrosome At TheC. elegansCiliary Base. J. Garbrecht, T. Laos, A. Dammermann;  Max Perutz Labs, University of Vienna, Vienna, AUSTRIA.

2:33 pm               SG270    Pericentrin‐like‐protein and Kinesin‐1 drive centriole motility for proper subcellular positioning.. M. R. Hannaford1, Z. T. Swider2, R. Liu1, N. Billington1, B. J. Galletta1, C. J. Fagerstrom1, J. R. Sellers1, N. M. Rusan1;  1NHLBI, NIH, Bethesda, MD, 2University of Wisconsin, Madison, WI.

2:48 pm               SG271    Centriolar satellites are required for efficient cilium assembly, maintenance and disassembly. O. Aydin, O. Taflan, C. Gurkaslar, E. Firat-Karalar;  Koc University, Istanbul, TURKEY.

3:03 pm                              Break

3:15 pm               SG272    A genetic screen for mechanisms that counter extra centrosomes. E. Jezuit, D. Fox;  Duke University Sch Med, Durham, NC.

3:30 pm               SG273    Understanding the impact of secretory alterations induced by centrosome amplification. S. Godinho;  Barts Cancer Institute, Queen Mary University London, London, UNITED KINGDOM.

3:45 pm               SG274    Inhibition of centrosome clustering reduces cystogenesis and improves kidney function in ADPKD. M. Mahjoub1, T. Cheng1, A. Mariappan2, E. Langner1, K. Shim1, J. Gopalakrishnan2;  1Washington Univ-St Louis, St Louis, MO, 2Heinrich-Heine University, Düsseldorf, GERMANY.

4:00 pm               SG275    orb2-dependent microcephaly reveals a novel role for RNA-binding proteins in centrosome regulation.. B. V. Robinson, D. A. Lerit;  Department of Cell Biology, Emory University School of Medicine, Atlanta, GA.

4:15 pm               SG276    Coordinated loss of cilia, centrioles and multi-ciliated cells during Xenopus development. R. Ventrella, S. Kim, B. Mitchell;  Cell and Developmental Biology, Northwestern University Feinberg Sch Med, Chicago, IL.

4:30 pm               SG277    SON splicing factor control of centriole assembly through modulation of centrosome trafficking and the cytoskeletal organization. A. J. Stemm-Wolf1, E. O'Toole2, R. M. Sheridan1, J. Morgan1, C. G. Pearson1;  1Cell and Developmental Biology, University of Colorado-SOM, Aurora, CO, 2MCDB, University of Colorado-Boulder, Boulder, CO.

Innate Immunity Across Systems: Connecting Structure to Function

Scientific Tracks: Specialized Cell and Evolution: Neurobiology, Immunology, and Emerging Model Systems

Organizers: Marcus Taylor, Max Planck Institute for Infection Biology; Olivia Majer, Freie Universität Berlin; and Jean L Scholz, University of Pennsylvania; 

The vision of this subgroup is to bring together a cross-disciplinary group of scientists who study innate immunity across diverse cellular systems. We aim to highlight unifying concepts in how the molecular and cellular mechanisms of innate immunity are organised from early metazoans to plants and mammals. We will focus on three areas: 1) Supramolecular organizing centers (SMOCs): The formation of SMOCs is emerging as a dominant mechanism of signal transduction used by inflammatory cytokine and pattern recognition receptors. This segment will focus on ongoing research investigating the molecular basis of the assembly of these macromolecular signaling organelles and how they organize signaling reactions in plant and metazoan cells. 2) Innate immune signaling and membrane trafficking: Innate immune recognition and signaling are deeply integrated into the subcellular architecture. This segment will highlight ongoing investigations of how cellular organization dictates the signaling behavior of innate immune receptors, and how immune challenges influence and remodel the cell’s architecture.  3) Innate immunity and SMOCs from A to P: Remarkable similarities exist in innate immune molecules, complexes, and pathogen responses in animals and plants, and phylogenetic analysis has revealed that the receptors and signaling domains of the eukaryotic innate immune system have an ancient origin.  This segment will highlight advances in understanding similarities and differences in innate immunity across kingdoms, and present current work characterizing the evolution and diversity of NLR immune receptors and complexes such as the inflammasome.  

1:45 pm                              Introduction by Marcus Taylor

1:50 pm               SG278    Signal Transduction Pathways of the Innate Immune System. J. Kagan;  Harvard Medical School and Boston Children's Hospital, Boston, MA.

2:05 pm               SG279    Visualisation of IL1 inflammatory signaling reveals the stepwise assembly of MyD88, IRAK4 and IRAK1 into Myddosomes. M. J. Taylor;  Max Planck Institute for Infection Biology, Berlin, GERMANY.

2:10 pm               SG280    Signalosome nucleation enables digital innate immune proinflammatory responses. A. Rodríguez Gama1, T. Miller1, J. Lange1, S. Venkatesan1, R. Halfmann1,2;  1Stowers Institute for Medical Research, Kansas City, MO, 2Molecular and Integrative Physiology, The University of Kansas School of Medicine, Kansas City, MO.

2:15 pm               SG281    DDX3X scaffold function promotes prionoid phase transition and NLRP3 inflammasome activation. P. Samir, S. Kesavardana, T. Kanneganti;  St Jude Children's Research Hospital, Memphis, TN.

2:20 pm               SG282    The cell biology of inflammasomes: localization, assembly and execution. H. Wu;  Harvard Medical School, Boston, MA.

2:35 pm                              Break

2:50 pm               SG283    Regulation of phagosomal size and integrity during infection. S. Grinstein, J. Westman, G. F. W. Walpole;  Hospital for Sick Children, Toronto, ON, CANADA.

3:05 pm               SG284    Cellular mechanisms of self versus non-self discrimination by nucleic acid-sensing Toll-like receptors. O. Majer1, B. Liu2, G. M. Barton2;  1Max Planck Institute for Infection Biology, Berlin, GERMANY, 2University of California, Berkeley, Berkeley, CA.

3:15 pm               SG285    A genome-wide CRISPR/Cas9 screen identifies novel regulators of GSDMD pore formation in engineered macrophages. C. L. Evavold1, I. Hafner-Bratkovic2, R. Jerala2, J. C. Kagan1;  1Program in Immunology, Harvard University, Boston, MA, 2Department of Synthetic Biology and Immunology, National Institute of Chemistry, Ljubljana, SLOVENIA.

3:20 pm               SG286    Nanoscale ligand patterns control macrophage phagocytosis. N. Kern1, M. Morrissey2, R. Vale1;  1University of California, San Francisco, San francisco, CA, 2University of California, Santa Barbara, Santa Barbara, CA.

3:25 pm               SG287    STING activation and signaling beyond interferon. N. Yan;  Department of Immunology, UT Southwestern Medical Center, Dallas, TX.

3:40 pm                              Break

3:55 pm               SG288    Interactions between intracellular and cell-surface immune receptor signalling pathways. J. D. G. Jones, P. Ding, B. Ngou, H. Ahn;  The Sainsbury Laboratory, Norwich, UNITED KINGDOM.

4:10 pm               SG289    Transcriptional induction of a broad anti-viral and anti-bacterial innate immune response in the starlet sea anemone Nematostella vectensis by the STING ligand 2'3'-cGAMP. S. R. Margolis, P. A. Dietzen, B. Remick, R. E. Vance;  Division of Immunology and Pathogenesis, Department of Molecular and Cell Biology, UC Berkeley, Berkeley, CA.

4:20 pm               SG290    Putting out the fire: an anti-inflammatory path to regeneration. N. Denans, D. Diaz, T. Piotrowski;  Stowers Institute of Medical Research, Kansas City, MO.

4:25 pm               SG291    Signaling by cooperative assembly formation (SCAF) by TIR domains in innate immunity and cell death pathways. B. Kobe1, T. Ve2, H. Burdett1, W. Gu1, J. Nanson1, S. Horsefield1, M. K. Manik1, P. Vajjhala1, P. N. Dodds3, K. Stacey1;  1School of Chemistry and Molecular Biosciences, University of Queensland, Australia, Brisbane, AUSTRALIA, 2Institute for Glycomics, Griffith University, Southport, AUSTRALIA, 3Agriculture and Food,, CSIRO, Cenberra, AUSTRALIA.

4:40 pm                              Wrap-Up