December 1-10, 2021
#cellbio2021
Special Interest Subgroups
Member-Organized Special Interest Subgroups take a deep dive into the seven scientific meeting tracks by exploring a wide range of specialty subjects under the umbrella of each topic area. These sessions are 2.5 hours in length and abstracts are submitted and selected for talks. Organizers may solicit speakers to submit abstracts, and are encouraged to select additional talks from other abstracts submitted to their session.
Subgroup sessions will be held December 6 through 10, 2021.
2021 Subgroup Sessions Schedule
Monday, December 6 | 1:45 PM - 4:15 PM EST
Scientific Track: Specialized Cell & Evolution
Organizers: Holly Goodson, University of Notre Dame, Michael McMurray, University of Colorado, Anschutz, Michelle Momany, University of Georgia, Masayuki Onishi, Duke University, Courtney Schroeder, Fred Hutchinson Cancer Research Center
The expansion of genomic and metagenomic data, discovery of new species, and development of novel model systems in recent years has brought new insights into the evolution of the cytoskeleton and cell division machinery. This session will highlight cell biology from bacteria, protists, algae, and fungi with an emphasis on the early-diverging lineages that give evolutionary insight to highly conserved cytoskeletal elements and their functions.
1:45 pm Introduction.
1:47 pm SG1 The archaeal origins of eukaryotic cell division. B. Baum; MRC-LMB, Cambridge, UNITED KINGDOM.
2:02 pm SG2 Activation of cytokinesis in Caulobacter crescentus. C. Mahone1, X. Yang2, J. Xiao2, E. Goley1; 1Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, 2Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD.
2:17 pm SG3 Cellular and molecular mechanisms of cell division and chromosome segregation in the thermoacidophilic crenarchaeon Sulfolobus acidocaldarius. A. Charles-Orszag, S. J. Lord, D. Mullins; Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA.
2:21 pm SG4 Evolution and function of divergent septin proteinsCo-Presenters: M. Onishi1, M. Momany2; 1Department of Biology, Duke University, Durham, NC, 2Fungal Biology Group and Plant Biology Department, University of Georgia, Athens, GA.
2:36 pm SG5 A gene duplication of a septin provides a developmentally-regulated filament length control mechanism. K. Cannon1, J. M. Vargas-Muniz2, N. Billington3, I. Seim1, J. Ekena1, J. Sellers3, P. Philippsen4, A. Gladfelter1; 1University of North Carolina at Chapel Hill, Chapel Hill, NC, 2Southern Illinois University-Carbondale, Carbondale, IL, 3National Institutes of Health, Bethesda, MD, 4University of Basel, Basel, SWITZERLAND.
2:51 pm SG6 Variation in Septin GTPase Activity as an Evolutionary Driver of Variation in Septin Filament Composition. M. A. McMurray; Cell and Developmental Biology, University of Colorado-Denver, Aurora, CO.
3:06 pm SG7 Characterization of Dictyostelium Cell-Substrate Adhesion Structures During Migration. J. Fierro Morales, M. Roh-Johnson; Biochemistry, University of Utah, Salt Lake City, UT.
3:10 pm SG8 Multiple Roles of the Actin Cytoskeleton in Giardia Attachment. A. Paredez, M. Steele-Ogus, W. Hardin; Biology, University of Washington, Seattle, WA.
3:25 pm SG9 A more complex Basal Complex: Mapping novel components of the Toxoplasma gondii cytokinesis machinery portrays an expanded hierarchy of its assembly. K. Engelberg1, T. Bechtel2, E. Weerapana2, M. Gubbels1; 1Biology, Boston College, Chestnut Hill, MA, 2Chemistry, Boston College, Chestnut Hill, MA.
3:40 pm SG10 Evolutionary Morphogenesis and the rise of The Fungi. E. M. Medina, S. M. Prostak, L. K. Fritz-Laylin; Biology, UMass Amherst, Amherst, MA.
3:55 pm SG11 The Hippo Pathway Regulates Cytoskeletal Dynamics in a Close Unicellular Relative of Animals. J. Phillips, D. Pan; UT Southwestern, Dallas, TX.
3:59 pm SG12 Ton1 and MyosinVIII mark the future site of cell division and control placement of the cell division plane. S. Wu1, D. Mallett2, M. Bezanilla1; 1Dartmouth College, Hanover, NH, 2University of Washington, Seattle, WA.
Scientific Tracks: Cellular Genome, Physical Cell
Organizers: Denis Discher, University of Pennsylvania, Rong Li, Mechanobiology Institute, Singapore
A cell's DNA sequence, particularly chromosome number and/or integrity, can be affected by more than intrinsic processes and include: Tissue architecture disruption, Mitotic confinement, Chromatin bridge breakage and force effects, Soft & stiff matrices in genome doubling, 3D folding and selection after migration. New methods are also being developed to assess such changes and their heritability with new reporters, and also the physical consequences of chromosome number changes.
1:45 pm SG13 Mechanical Microenvironment, Cytokinesis Failure, and Multinucleation. C. M. Nelson; Princeton University, Princeton, NJ.
2:00 pm SG14 The mitotic actin cytoskeleton and endoplasmic reticulum morphology orchestrate patterns of nuclear envelope assembly. G. Zhao*1,2,3, S. Liu*1,2,3, S. Arun1,2,3, F. Renda4, A. Khodjakov4, D. Pellman1,2,3; 1Howard Hughes Medical Institute, Chevy Chase, MD, 2Department of Pediatric Oncology, Dana-Farber Cancer Institute, Boston, MA, 3Department of Cell Biology, Harvard Medical School, Boston, MA, 4Wadsworth Center, New York State Department of Health, Albany, NY.
2:15 pm SG15 The relationship between constricted migration and 3D genome organization. R. Golloshi1, C. Playter1, K. Perry1, P. Das2, T. Freeman1, R. P. McCord1; 1Biochemistry & Cellular and Molecular Biology, University of Tennessee - Knoxville, TN, Knoxville, TN, 2Genome Science and Technology Graduate Program, University of Tennessee - Knoxville, TN, Knoxville, TN.
2:30 pm SG16 Cellular Responses to Chronic Osmotic Stress. B. S. Wong, K. Sethi, R. Li; National University of Singapore, Singapore, SINGAPORE.
2:45 pm SG17 Oncogenic Ras alters cell shape and mechanics in mitosis to promote loss of tissue integrity and the proliferation of individual cells. H. Matthews1,2, S. Ganguli2, A. Nyga3, B. Baum3; 1University of Sheffield, Sheffield, UNITED KINGDOM, 2University College London, London, UNITED KINGDOM, 3MRC Laboratory for Molecular Biology, Cambridge, UNITED KINGDOM.
3:00 pm SG18 3d-stiffness maximizes heritable dna changes as revealed with live cell creporters . D. E. Discher; University Pennsylvania, philadelphia, PA.
3:15 pm SG19 Kras and mechanical compression drive evolution toward a pancreatic cancer genotype. G. P. Brittingham, 2, S. Saxena, 1, G. R. Kidiyoor, 1, S. Keegan, 1, S. Tsao, 1, L. J. Holt, 1; New York University, New York, NY.
3:30 pm SG20 Mechanisms of Inherently Low Fidelity of Chromosome Segregation in Human Pluripotent Stem Cells. C. Deng, A. Ya, D. Compton, K. Godek; Department of Biochemistry and Cell Biology, Geisel School of Medicine at Dartmouth College, Lebanon, NH.
3:35 pm SG21 Delivering insights into organ homeostasis and regeneration through in vivo genome-wide screens. H. R. Keys1, K. A. Knouse2; 1Whitehead Institute for Biomedical Research, Cambridge, MA, 2Massachusetts Institute of Technology, Cambridge, MA.
3:50 pm SG22 Members of the MRE11-NBS1-RAD50 complex promote proper kinetochore-microtubule attachments and faithful chromosome segregation in a DNA damage-independent manner. E. M. Black1,2, L. Kabeche1,2; 1Molecular Biophysics and Biochemistry, Yale University, New Haven, CT, 2Cancer Biology Institute, Yale University, New Haven, CT.
3:55 pm SG23 Force generation of KIF1C is impaired by pathogenic mutations. N. Siddiqui, D. Roth, A. Toleikis, A. Zwetsloot, R. Cross, A. Straube; Centre for Mechanochemical Cell Biology, University of Warwick, Coventry, UNITED KINGDOM.
4:00 pm SG24 Synergistic Killing of Ovarian Cancer Cells by SYK Inhibitor and Paclitaxel via Disruption of Nuclear Envelope Integrity. J. Chen1, M. Wang1, T. Wang2,3,4, I. Shih2,3,4, Y. Chen1,5,6; 1Mechanical Engineering, Johns Hopkins University, Baltimore, MD, 2Department of Gynecology and Obstetrics, Johns Hopkins Medical Institutions, Baltimore, MD, 3Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, MD, 4Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins Medical Institutions, Baltimore, MD, 5The Institute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD, 6Center for Cell Dynamics, Johns Hopkins University, Baltimore, MD.
4:05 pm SG25 Single-cell Analysis Reveals Predictable Quantitative Patterns of DNA Damage Checkpoint Adaptation. A. Sadeghi, R. Dervey, V. Gligorovski, S. Rahi; EPFL, Lausanne, SWITZERLAND.
4:10 pm SG26 Improved transcript discovery in single cell RNA-Seq with CRISPRclean. J. Armstrong1, A. Siddique1, G. Suckow1, J. Carey2, P. Ordoukhanian3, K. Brown1, S. Head3, S. Nelson4, D. Evans3, A. Torkamani3, T. Alsaigh3; 1Jumpcode Genomics, San Diego, CA, 2Fulcrum Genomics, Phoenix, AZ, 3Scripps Research Institute, La Jolla, CA, 4David Geffen School of Medicine, UCLA, Los Angeles, CA.
Scientific Tracks: Physical Cell, Cellular Dynamics
Organizers: Sophie Dumont, University of California San Francisco, Alexander Dunn, Stanford University
The construction of all living things necessarily progresses from molecules, upward. However, understanding how the intricate structures of cells and tissues arise from molecular-scale interactions remains a preeminent challenge for experimentalists and theorists alike. Specifically, while powerful tools exist for defining the structures of both macromolecular complexes and cellular tissues, subcellular structures on the intermediate, submicron length-scale are considerably more challenging to understand due to their compositional complexity, dynamic nature, and resistance to reconstitution approaches. This challenge applies not only to the cytoskeleton, where it has been studied most extensively, but to virtually every aspect of subcellular organization. This session showcases recent work aimed at understanding how cells build mesoscale, subcellular structures that are comprised of hundreds to millions of macromolecules, but that are still small compared to cells and tissues. We hope to show how a convergence of experimental and theoretical approaches can shed light on the composition, structure, and dynamics of subcellular assemblies that fall in the “messy micron.” In so doing, we will showcase some of the most exciting, up-and-coming members of the cell biological community.
1:45 pm Introduction.
1:48 pm SG27 Combinatorial codes of mitochondrial nucleoid composition govern the regulation of mtDNA replication. S. Lewis; University of California, Berkeley, Berkeley, CA.
2:00 pm SG28 Building axonal branches: Molecular insights from cryo-electron tomography. S. Bodakuntla1, H. Nedozralova1, N. Basnet1, I. Ibiricu2, C. Biertümpfel1, N. Mizuno1; 1NHLBI, Bethesda, MD, 2MPI, Martinsried, GERMANY.
2:12 pm SG29 E-cadherin adhesion assembly and signaling on synthetic membrane substrates. K. H. Biswas; College of Health & Life Sciences, Hamad Bin Khalifa University, Doha, QATAR.
2:24 pm SG30 A cellular mechanochemical wave of contraction during tissue morphogenesis. T. Lecuit; IBDM, Marseille, FRANCE.
2:36 pm SG31 Actin Organization and Dynamics in MotileToxoplasma gondii Parasites. C. Hueschen1, L. Segev Zarko1, J. Chen2,3, M. LeGros2,3, C. Larabell2,3, J. Boothroyd1, R. Phillips4, A. R. Dunn1; 1Stanford, Palo Alto, CA, 2UCSF, San Francisco, CA, 3Lawrence Berkeley National Laboratory, Berkeley, CA, 4Caltech, Pasadena, CA.
2:48 pm Break.
3:03 pm SG32 Polar chromosomes in human cells congress by microtubule pivoting. I. Koprivec, V. Stimac, I. M. Tolic; Division of Molecular Biology, Rudjer Boskovic Institute, Zagreb, CROATIA.
3:15 pm SG33 Mammalian Kinetochore-Fibers Regulate Their Length and Dynamics Individually, Independent of Spindle Poles. M. Richter, S. Dumont; UCSF, San Francisco, CA.
3:27 pm SG34 Mechanism of kinetochore fiber maturation by Augmin. A. C. Almeida1, J. S. Oliveira1, D. Drpic1, L. P. Cheeseman1, J. Damas2, H. A. Lewin2, D. M. Larkin3, P. Aguiar1, A. J. Pereira1, H. Maiato1; 1Instituto de Investigação e Inovação em Saúde (i3S), Porto, PORTUGAL, 2Department of Evolution and Ecology, University of California, Davis, Davis, CA, 3Department of Comparative Biomedical Sciences, Royal Veterinary College, University of London, London, UNITED KINGDOM.
3:39 pm SG35 A gelation transition enables the self-organization of bipolar metaphase spindles. B. Dalton, D. Oriola, F. Decker, F. Julicher, J. Brugues; Max Planck Institute, Dresden, GERMANY.
3:51 pm SG36 Real-time Chromatin Assembly on Naked DNA in Xenopus Egg Extract. M. Sun, H. Amiri, R. Heald; University of California, Berkeley, Berkeley, CA.
4:03 pm SG37 Nucleosomes as liquid-like organisers of chromatin. R. Collepardo; University of Cambridge, Cambridge, UNITED KINGDOM.
Scientific Tracks: Cells in Distress & Disease, Cellular Dynamics
Organizers: Delphine Muriaux, CNRS & University of Montpellier, France, Prabuddha Sengupta, HHMI, Erdinc Sezgin, SciLifeLab, Karolinska Institutet, Sweden
Enveloped viruses, which include HIV and coronaviruses such as SARS-CoV-2, have an outer membrane acquired from host cell membranes, which serves as a critical structural component of the viruses. Recent studies support the idea that the composition and physical properties of viral membranes are optimized to facilitate productive particle assembly and interactions with target cell membranes. We will address two key questions regarding virus membrane in this subgroup. How and why do diverse viruses acquire their unique membrane composition? How do the specific composition and resulting biophysical properties of virus membranes regulate their particle production and interactions with host cells?
Superresolution microscopy is being increasingly used to study the sub-diffraction sized enveloped viruses, generating unprecedented insights about virus assembly and their interactions with host cells. These studies underline the importance of interdisciplinary collaborations between virologists, cell biologists, membrane biophysicists, and microscopists for addressing these questions. We anticipate that the subgroup will catalyze intellectual exchanges between these complementary fields to address questions about virus assembly and entry relevant for understanding human health and disease. Additionally, since viruses often utilize host cell pathways, we expect that ideas emerging from the subgroup will be relevant for understanding membrane organization within mammalian cells.
1:45 pm Introduction.
1:47 pm SG38 Lipid-dependent budding and spread of emerging pathogenic viruses. R. V. Stahelin; Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, IN.
2:05 pm SG39 Understanding Influenza A virus assembly using quantitativefluorescence microscopy. A. Petrich, S. Bobone, V. Dunsing, S. Chiantia; University of Potsdam, Potsdam, GERMANY.
2:23 pm SG40 Nanophysiology of Influenza Virus Infection. C. Sieben; Helmholtz Centre for Infection Research, Braunschweig, GERMANY.
2:41 pm SG41 A BECLIN1-DERIVED PEPTIDE PROMOTES VIRUS ENTRY IN EARLY ENDOSOMES BY ACTIVATING THE BECLIN1-VPS34-ATG14L COMPLEX. S. MAJDOUL, K. Rahman, G. Shi, A. A. Compton; HIV Dynamics and Replication Program, Center for Cancer Research, National Cancer Institute, NIH, Frederick, MD.
2:56 pm SG42 S-acylation controls SARS-Cov-2 membrane lipid organization and enhances infectivity. F. S. Mesquita1, L. Abrami1, O. Sergeeva1, P. Turelli1, E. Qing2, B. Kunz1, C. Raclot1, J. Paz Montoya3, L. Abriata3, T. Gallagher2, M. Dal Pararo3, D. Trono1, G. D'Angelo3, G. van der Goot1; 1Global Health Institute, School of Life Sciences, EPFL, Lausanne, SWITZERLAND, 2Department of Microbiology and Immunology, Loyola University, Chicago, IL, 3Institute of Bioengineering, School of Life Sciences, EPFL, Lausanne, SWITZERLAND.
3:11 pm Break.
3:21 pm SG43 Sneaking out with the trash: How Coronaviruses get out of cells. N. Altan-Bonnet; National Heart, Lung, and Blood Institute, NIH, Bethesda, MD.
3:39 pm SG44 The Roles Played by the Plasma Membrane Phospholipids in HIV-1 Particle Assembly and Host Protein Incorporation. R. de Souza Cardoso1, T. Murakami1, S. L. Veatch2, A. Ono1; 1Microbiology & Immunology, University of Michigan, Ann Arbor, MI, 2Biophysics, University of Michigan, Ann Arbor, MI.
3:57 pm SG45 How physics assists in assembling HIV. P. Bassereau1, F. Tsai1, K. Inamdar2, C. Favard2, D. Muriaux3; 1Physical Chemistry Curie, Institut Curie, Paris, FRANCE, 2IRIM-CNRS, Montpellier, FRANCE, 3IRIM-CNRS, Paris, FRANCE.
Scientific Tracks: Cellular Dynamics, Signaling & Metabolism
Organizers: Farah Haque, Massachusetts General Hospital, Harvard Medical School, Agnieszka Kendrick, University of California, San Diego, Le Ma, Thomas Jefferson University, Radhika Subramanian, Harvard Medical School
Specialized microtubule arrays are essential for diverse cellular functions, including migration, division, morphogenesis and signaling. This session will be dedicated to two fundamental aspects of microtubule array formation and function. The first half of this special subgroup session will focus on mechanisms of microtubule-based microtubule transport, a process ubiquitously involved in microtubule array organization. The second half will focus on mechanisms by which microtubules regulate the output of diverse cellular signaling events, highlighting the reciprocal flow of information between microtubule and signal transduction networks. Altogether, we aim to highlight the less appreciated roles of microtubules as cargoes and signaling hubs important for cellular morphology and physiology.
1:45 pm Introduction.
1:48 pm SG46 Microtubule Sliding in the Axon:an Old Topic Meets the Future. P. Baas; Neurobiology and Anatomy, Drexel University College Med, Philadelphia, PA.
2:00 pm SG47 The Role of Kinesin-1 Mediated Microtubule Sliding in Regulation of Insulin Secretion. K. Bracey1, M. Fye1, H. McKinney1, W. R. Holmes2, G. Gu1, I. Kaverina1; 1Cell and Developmental Biology, Vanderbilt University, Nashville, TN, 2Physics and Astronomy, Vanderbilt University, Nashville, TN.
2:12 pm SG48 Map7 anchors kinesin-1 to slide parallel and antiparallel microtubules. B. Yang1, S. Tymanskyj1, K. Verhey2, L. Ma1; 1Thomas Jefferson University, Philadephia, PA, 2University of Michigan Medical School, Ann Arbor, MI.
2:24 pm SG49 Deciphering the mechanics of crosslinked microtubule networks in mitosis. S. T. Forth, I. Gaska, A. Alfieri, M. Armstrong; Rensselaer Polytechnic Institute, Troy, NY.
2:36 pm SG50 Kinesin-6 Klp9 orchestrates spindle elongation by regulating microtubule sliding and growth. L. K. Krüger1, M. Gélin2, L. Ji1, C. Kikuti1, A. Houdusse1, M. Théry2,3, L. Blanchoin3,2, P. Tran1; 1Institut Curie, Paris, FRANCE, 2Institut de Recherche Saint Louis, Paris, FRANCE, 3Interdisciplinary Research Institute Grenoble, Grenoble, FRANCE.
2:48 pm SG51 Motor guidance by long-range communication through the microtubule highway. M. Betterton1, S. Wijeratne2, S. Fiorenza1, R. Subramanian2; 1Physics, University of Colorado-Boulder, Boulder, CO, 2Massachusetts General Hospital, Boston, MA.
3:00 pm SG52 Microtubule Dynamics During Plant Cell Division Plane Orientation. C. G. Rasmussen1, M. A. Bellinger1, A. N. Uyehara1, P. Martinez1, M. C. McCarthy2; 1Botany and Plant Sciences, University of California, Riverside, Riverside, CA, 2Radical Research LLC, Riverside, CA.
3:12 pm SG53 Cytoplasmic dynein-1 cargo diversity is mediated by the combinatorial assembly of FTS-Hook-FHIP complexes. A. Kendrick, J. Christensen, J. Truong, A. Aguilar-Maldonado, V. Adani, S. Reck-Peterson; University of California, San Diego, San Diego, CA.
3:24 pm SG54 Cytoskeletal regulation of a transcription factor by DNA mimicry. F. Haque1,2, C. Freniere1,2, Q. Ye1,2, N. Mani1,2, P. Ku1,2, E. M. Wilson-Kubalek3, R. A. Milligan3, R. Subramanian1,2; 1Massachusetts General Hospital, Boston, MA, 2Harvard Medical School, Boston, MA, 3Scripps Research, La Jolla, CA.
3:36 pm SG55 Dynein acts to cluster glutamate receptors and traffic the PIP5 kinase, Skittles, to regulate postsynaptic membrane organization at the neuromuscular junction. A. L. Neisch1, T. Pengo2, A. W. Avery3,1, M. Li1, T. S. Hays1; 1Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, MN, 2Informatics Institute, University of Minnesota, Minneapolis, MN, 3Chemistry, Oakland University, Rochester, MI.
3:48 pm SG56 Regulation of the ciliary proteome by ubiquitin. S. Shinde, T. Das, M. V. Nachury; UCSF, San Francisco, CA.
4:00 pm SG57 Multiple motor effector proteins drive autophagosomal transport in axons. S. E. Cason, P. J. Carman, C. Van Duyne, J. Goldsmith, R. Dominguez, E. L. F. Holzbaur; University of Pennsylvania, Philadelphia, PA.
Scientific Tracks: Communal Cell, Physical Cell
Organizers: Young-wook, Jun, University of California San Francisco, Minsuk Kwak, Yonsei University, Matthew Paszek, Cornell University
Juxtacrine signaling mediates communications via physical contact between neighboring cells, orchestrating development and physiology of multicellular organisms. The signal-exchange interfaces create unique microenvironments that biophysically constrain the arrangement and activity of protein, lipid, and glycan components, finally mediating the transport, spatial organization, and biochemical activity of surface receptors and ligands, serving as the basis for wide-range of cellular functions. Recent studies have strongly emphasized the critical regulatory roles of physical and mechanical properties of the juxtacrine signaling molecules and their environments in cell signaling and function. Multiple physical parameters, including the size, charge, mechanical loading, and entropy, of the cell-cell interfacial components have emerged as key variables in the spatial organization and downstream signaling of receptors.
This subgroup will discuss the emerging physical approaches to dissect, interrogate, and understand the molecular mechanisms underlying juxtacrine cell-cell signaling with specific emphasis on:
- Membrane biophysics facilitating receptor organization and signaling at the cell-cell contact
- Spatial dynamics of cell-cell interfacial proteins at the mechanical, immunological, and neurological synapses
- Mechanotransduction between cells towards tissue development and disease
- Glycocalyx biophysics regulating cell-cell interface and signaling.
1:45 pm Introduction.
1:48 pm SG58 The molecular basis of cell-cell recognition. B. Honig; Systems Biology, Columbia University, New York, NY.
2:05 pm SG59 Structural and functional basis of the transsynaptic teneurin-latrophilin complex. D. Araç1, J. Li1, Y. Xie1, R. Sando1, T. Sudhof2, M. Zhao1; 1University of Chicago, Chicago, IL, 2Stanford University, Stanford, CA.
2:22 pm SG61 The molecular specificity of JAM-C on cerebellar granule neurons connects adhesive recognition to migration in a mouse model for cerebellar development. L. P. Hallada1, D. J. Solecki2; 1Developmental Neurobiology, St Jude Graduate School of Biomedical Sciences, Memphis, TN, 2Developmental Neurobiology, St Jude Children's Research Hospital, Memphis, TN.
2:33 pm SG62 Adherens junction-mediated membrane compartmentalization creates a spatial switch for Notch signaling and function. M. Kwak1, K. M. Southard2, W. Kim2, Z. J. Gartner3, Y. Jun2; 1Center for Nanomedicine, IBS, Yonsei University, Seoul, KOREA, REPUBLIC OF, 2Department of Otolaryngology, UCSF, San Francisco, CA, 3Department of Pharmaceutical Chemistry, UCSF, San Francisco, CA.
2:50 pm Break.
2:56 pm SG63 Measuring cell surface molecular heights and barriers to cell-cell contacts. S. Son, D. Fletcher; University of California, Berkeley, Berkeley, CA.
3:13 pm SG64 The septate junction protein Bark beetle is required for Drosophila intestinal barrier function and homeostasis. R. Hodge1, E. Edmond1, F. de la Torre1, M. Resnik-Docampo2, L. Jones3; 1Molecular, Cellular, and Developmental Biology, University of California, Los Angeles, Los Angeles, CA, 2BioMed X, Heidelberg, GERMANY, 3Anatomy, University of California, San Fransisco, Los Angeles, CA.
3:24 pm SG65 A positive feedback loop between mesendoderm cell migration and interstitial fluid relocalization is required for embryonic axis formation in zebrafish. K. Huljev, C. Heisenberg; Inst Sci/Technol Austria, Klosterneuburg, AUSTRIA.
3:41 pm SG66 The distinct roles of cell and tissue mechanics in vertebrate body axis elongation. O. Campas1,2; 1Physics of Life Excellence Cluster, TU Dresden, Dresden, GERMANY, 2University of California, Santa Barbara, Santa Barbara, CA.
3:58 pm SG67 Physical regulation of intercellular communication by the cellular glycocalyx. M. Paszek; Cornell University, Ithaca, NY.
Tuesday, December 7 | 1:45 PM - 4:15 PM EST
Scientific Tracks: Cellular Dynamics, Physical Cell
Organizers: Mark Chan, San Francisco State University, Susanne Rafelski, Allen Institute for Cell Science
Modern cell biology has made great strides in understanding cell structure and function. Cells also face an important engineering challenge: assembly. How are the complex three-dimensional structures found within the cell specified and regulated by instructions from a one-dimensional genome? In Building the Cell we explore this question, which lies at the interface of biology and physics. This session will be highly interdisciplinary with speakers whose interests span physics, mathematical modeling, biochemistry, cell biology and more. This year the entire subgroup agenda will be based on submitted abstracts.
1:45 pm Introduction.
1:47 pm SG68 Phase behavior and function of mitochondrial transcriptional condensates. M. Feric1, A. Sarfallah2, F. Dar3, R. Pappu3, D. Temiakov2, T. Misteli1; 1National Cancer Institute (NCI), National Institutes of Health (NIH), Bethesda, MD, 2Thomas Jefferson University, Philadelphia, PA, 3Washington University in St. Louis, St. Louis, MO.
2:02 pm SG69 Entangled Architecture of Rough Endoplasmic Reticulum (RER) and Vacuoles Enables Topological Brakes in Cytoplasm of an Ultra-fast Giant Cell. R. Chang, M. Prakash; Bioengineering, Stanford University, Stanford, CA.
2:17 pm SG70 Mitotic Spindle Chirality Provides a Passive Mechanical Response to Forces and Depends on Microtubule Motors and Crosslinkers. M. Trupinic, B. Kokanovic, I. Ponjavic, I. M. Tolic; Ruder Boskovic Institute, Zagreb, CROATIA.
2:32 pm SG71 Cytoplasmic Trade Winds Push Actin Polymerization to the Leading Edge. C. G. Galbraith1, U. Boehm2, J. A. Galbraith1; 1OHSU, Portland, OR, 2Janelia Research Campus, Ashburn, VA.
2:47 pm SG72 A pooled single-cell CRISPRi screen for regulators of U2OS cell shape and actin organization. R. D. Labitigan1,2, A. L. Sanborn3, C. V. Hao3, C. K. Chan4, J. Brown4, M. Mehrotra5, J. A. Theriot2,6; 1Department of Biochemistry, Stanford University, Stanford, CA, 2Department of Biology, University of Washington, Seattle, WA, 3Department of Computer Science, Department of Structural Biology, Stanford University, Stanford, CA, 4Allen Institute for Cell Science, Seattle, WA, 5Information School, Department of Biology, University of Washington, Seattle, WA, 6Howard Hughes Medical Institute, Seattle, WA.
3:02 pm Break.
3:15 pm SG73 Measuring the Spatial Organization of Signaling on the 3D Cell Surface. H. Mazloom Farsibaf1, R. Hsieh2, G. Danuser1, M. Driscoll1; 1UT Southwestern Medical Center, Dallas, TX, 2Voiland School of Chemical Engineering and Bioengineering, Washington State University, Pullman, WA.
3:30 pm SG74 Mapping cell structure across scales by fusing protein images and interactions. Y. Qin1, C. F. Winsnes2, E. L. Huttlin3, F. Zheng1, W. Ouyang2, J. Park1, A. Pitea1, J. F. Kreisberg1, S. P. Gygi3, W. Harper3, J. Ma4, E. Lundberg2, T. Ideker1; 1University of California San Diego, La Jolla, CA, 2KTH-Royal Institute of Technology, Stockholm, SWEDEN, 3Harvard Medical School, Boston, MA, 4Purdue University, West Lafayette, IN.
3:45 pm SG75 Uncovering Mechanistic Rules that Drive Emergent Cell Shape and Colony Dynamics Through Agent-Based Modeling. J. S. Yu1, . Allen Institute for Cell Science1, G. Johnson1, N. Bagheri1,2; 1Allen Institute for Cell Science, Seattle, WA, 2University of Washington, Seattle, WA.
4:00 pm SG76 Emergence of synchronized multicellular mechanosensing from spatiotemporal integration of heterogeneous single-cell information transfer. A. Zamir1, G. Li2, K. Chase3, R. Moskovitch1, B. Sun2, A. Zaritsky1; 1Ben-Gurion University of the Negev, Beer Sheva, ISRAEL, 2Oregon State University, Corvallis, OR, 3Princeton University, Princeton, NJ.
Scientific Tracks: Specialized Cell & Evolution, Physical Cell
Organizers: David Booth, University of California, San Francisco, Ben Larson, University of California, San Francisco, Guillermina Ramirez-San Juan, Brandeis University
Across the tree life, cells navigate constantly fluctuating environments with diverse and sophisiticated forms, behaviors, and life histories. Inspired by the diversity of cells in their natural environment, the WILD, this subgroup will focus on the use of non-traditional systems and related technologies to address fundamental questions in cell biology. The diversity of perspectives and systems represented in this subgroup will expand the boundaries of cell biology by combining evolutionary, physical, and ecological research.
1:45 pm Introduction.
1:50 pm SG77 Variation in resistance of Paramecium cells to an endonuclear parasite: geographic and lineage-specific patterns. J. Weiler1, G. Zilio2, N. Zeballos2, L. Noergaard3, W. Conce Alberto4, S. Krenek5, O. Kaltz2, L. Bright1; 1Biology, State University of New York at New Paltz, New Paltz, NY, 2ISEM, University of Montpellier, Montpellier, FRANCE, 3School of Biological Sciences and Center for Geometric Biology, Monash University, Melbourne, AUSTRALIA, 4Division of Infectious Diseases, Department of Medicine, Weill Cornell Medicine, New York, NY, 5Institute of Hydrobiology, Technische Universitat Dresden, Dresden, GERMANY.
2:02 pm SG78 Single-cell transcriptomics and genomics are key methods to understanding the evolutionary diversity of protists. G. Lax; Department of Botany, University of British Columbia, Vancouver, BC, CANADA.
2:14 pm SG79 Hydrodynamic influence of extracellular structures in heterotrophic flagellates. S. S. Asadzadeh1, J. H. Walther2,3, T. Kiørboe1; 1National Institute of Aquatic Resources and Centre for Ocean Life, Technical University of Denmark, Lyngby, DENMARK, 2Department of Mechanical Engineering, Technical University of Denmark, Lyngby, DENMARK, 3Computational Science and Engineering Laboratory, Swiss Federal Institute of Technology Zürich, Zürich, SWITZERLAND.
2:26 pm Break.
2:36 pm SG80 The Effect of Mucus-like Viscoelastic Stress on Giardia Growth. S. Li1, J. Miller2, H. Paynter2, D. Gagnon2, K. Watanabe3, J. Urbach2, H. Elmendorf1; 1Biology, Georgetown University, Washington, DC, 2Physics, Georgetown University, Washington, DC, 3Claremont McKenna College, Claremont, CA.
2:48 pm SG81 Molecular Swiss Army Knives:Tardigrade CAHS Proteins Mediate Desiccation Tolerance Through Multiple Mechanisms. T. Boothby; University of Wyoming, Laramie, WY.
3:00 pm SG82 Cold-responsive progenitors from the vascular smooth muscle lineage are a critical source of thermogenic adipocytes. F. Shamsi1,2, M. Piper3, L. Ho4, T. Huang2, A. Gupta5, V. Efthymiou2, A. Streets6,7, A. P. White8, M. Patti2, M. D. Lynes9,2, Y. Tseng2,10; 1Molecular Pathobiology, New York University, New York, NY, 2Section on Integrative Physiology and Metabolism, Joslin Diabetes Center, Harvard Medical School, Boston, MA, 33Department of Biostatistics, Harvard T.H. Chan School of Public Health, Boston, MA, 4Computer Science and Artificial Intelligence Laboratory, Massachusetts Institute of Technology, Boston, MA, 5UC Berkeley-UC San Francisco Graduate Program in Bioengineering, Berkeley, CA, 6Department of Bioengineering, University of California, Berkeley, Berkeley, CA, 7Chan-Zuckerberg Biohub, Francisco, CA, 8Department of Orthopedic Surgery, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, 9Maine Medical Center Research Institute, Scarborough, ME, 10Harvard Stem Cell Institute, Harvard University, Cambridge, MA.
3:12 pm Break.
3:22 pm SG83 Chemotactic responses in infectious life stages of the fungal pathogen B. dendrobatidis alter spore distribution throughout the environment. A. J. M. Swafford, G. L. Reynolds, L. K. Fritz-Laylin; University of Massachusetts, Amherst, Amherst, MA.
3:34 pm SG84 Hydrostatic pressure drives cell separation and fluid uptake during wound healing in zebrafish epidermis. A. S. Kennard1, M. Sathe2, E. Labuz3, C. Prinz2, J. Theriot2; 1Biology, University of Massachusetts, Amherst, MA, 2Biology, University of Washington, Seattle, WA, 3Biophysics, Stanford University, Stanford, CA.
3:46 pm SG85 Phenotyping perceptive protists. S. A. Bentley, V. Anagnostidis, H. Laeverenz Schlogelhofer, F. Gielen, K. Y. Wan; Living Systems Institute, University of Exeter, Exeter, UNITED KINGDOM.
3:58 pm Break.
Scientific Tracks: Physical Cell, Specialized Cell & Evolution
Organizers: Kristina Godek, Geisel School of Medicine at Dartmouth, Satoshi Namekawa, University of California, Davis, Bernhard Payer, CRG, Eduardo Torres, University of Massachusetts Medical School, Eda Yildirim, Duke University
Genome function relies on the organization of genetic material into chromosomes and the equal partitioning of chromosomes during cell division to ensure faithful transmission. Accordingly, deciphering how chromosome structure, dynamics, and copy numbers influence cellular processes ranging from gene regulation, to genome stability, and the execution of developmental programs is essential for our understanding of organism health and survival. The field of chromosome biology exploits approaches ranging from cell, molecular, and structural biology to biophysics, genomics, imaging and computational modelling. The aim of this Special Interest Subgroup is to bring together a diverse group of international researchers to discuss the underlying mechanisms, models, and advances of chromosome dynamics and chromosome structure in epigenetic gene regulation, dosage compensation, and aneuploidy in germline, development and disease.
1:45 pm SG86 Chromatin compartment formation via chromatin-binding phase-separating proteins. A. R. Strom, J. Eeftens, D. Bracha, N. Orlovsky, C. P. Brangwynne; Princeton University, Princeton, NJ.
2:00 pm SG87 Replication timing maintains the global epigenetic state in human cells. D. M. Gilbert; San Diego Biomedical Research Institute, San Diego, CA.
2:15 pm SG88 Maternal epigenetic inheritance by Polycomb repressive complexes. A. Inoue; Integrative Medical Sciences, RIKEN, Yokohama, JAPAN.
2:30 pm SG89 RARE competition: Regulation of Hoxb nascent transcripts by multiple RAREs. Z. Afzal, J. Lange, S. McKinney, C. Wood, C. Nolte, B. De Kumar, J. Unruh, B. Slaughter, R. Krumlauf; Stowers Institute for Medical Research, Kansas City, MO.
2:45 pm SG90 A cycle of X-chromosome inactivation and reactivation demarcates mouse in vitro germ cells with meiotic and oogenic potential. J. Severino1, M. Bauer1, T. Mattimoe1, N. Arecco1, L. Cozzuto1, P. Lorden2, N. Hamada3, Y. Nosaka4, S. Nagaoka4, H. Heyn2, K. Hayashi3, M. Saitou4, B. Payer1; 1Centre for Genomic Regulation (CRG), Barcelona, SPAIN, 2CNAG-CRG, Barcelona, SPAIN, 3Kyushu University, Fukuoka, JAPAN, 4Kyoto University, Kyoto, JAPAN.
3:00 pm Break.
3:15 pm SG91 Mechanisms that promote acentrosomal spindle assembly and stability during oocyte meiosis. T. J. Mullen, G. Cavin-Meza, I. D. Wolff, E. R. Czajkowski, N. S. Divekar, S. M. Wignall; Northwestern University, Evanston, IL.
3:30 pm SG92 Genomic inference of the origins of aneuploidy in human preimplantation embryos. D. Ariad1, M. R. Starostik1, O. A. Sosina2, S. M. Yan1, A. R. Victor3, F. L. Barnes3, C. G. Zouves3, M. Viotti4, R. C. McCoy1; 1Department of Biology, Johns Hopkins University, Baltimore, MD, 2Department of Biostatistics, Johns Hopkins University, Baltimore, MD, 3Zouves Fertility Center, Foster City, CA, 4Zouves Foundation for Reproductive Medicine, Foster City, CA.
3:45 pm SG93 Parental genome unification is highly error-prone in mammalian embryos. T. Cavazza1, Y. Takeda2, A. Z. Politi1, M. Aushev2, P. Aldag3, C. Baker4, M. Meenakshi Choudhary5, J. Bucevicius1, G. Lukinavicius1, K. Elder4, M. Blayney4, A. Lucas-Hahn3, H. Niemann3, M. Herbert2, M. Schuh1; 1Max Planck Institute for Biophysical Chemistry, Goettigen, GERMANY, 2Wellcome Centre for Mitochondrial Research, Newcastle upon Tyne, UNITED KINGDOM, 3Institute of Farm Animal Genetics, Biotechnology, Friedrich-Loeffler-Institute, Mariensee, GERMANY, 4Bourn Hall Clinic, Cambridge, UNITED KINGDOM, 5Newcastle Fertility Centre at Life, Newcastle upon Tyne, UNITED KINGDOM.
4:00 pm SG94 Aneuploidy disrupts cellular physiology and metabolism. E. M. Torres, S. Hwang; Molecular, Cell and Cancer Biology, University of Massachusetts Medical School, worcester, MA.
Scientific Tracks: Cellular Dynamics, Signaling & Metabolism
Organizers: Anna Kashina, University of Pennsylvania, Riyad Seervai, Baylor College of Medicine, Kristen Verhey, University of Michigan
With each passing year, an expanding body of research brings us closer to understanding how the cytoskeleton affects cellular processes ranging from mitosis and meiosis to motility and migration. Posttranslational modifications have often been referred to as the “Cinderella of the cytoskeleton” due to their significant yet undervalued role in affecting cytoskeletal dynamics. There is an increasing focus on how PTMs change the structure of cytoskeletal proteins and their interaction with other elements of the cell. The emerging functions of individual actin and tubulin isoforms, previously thought to be redundant, has added a layer of complexity to this “cytoskeleton code,” analogous to the “histone code” on chromatin. As such, these modifications serve as potential targets for therapies related to neurodegeneration, cardiovascular disease, and cancer. In this subgroup, we will compare and contrast the role of different mechanisms in regulating cytoskeletal proteins, and explore how they form a coherent language to diversify and direct the cytoskeletal network within the cell.
1:45 pm Introduction.
1:50 pm SG95 Regulation of cytoplasmic actins at the nucleotide and amino acid level. P. Vedula1, S. Kurosaka2, B. MacTaggart3, Q. Ni4, G. Papoian5, Y. Jiang6, D. W. Dong7, A. Kashina8; 1Department of Biomedical Sciences, University of Pennsylvania, Philadelphia, PA, 2Institute of Advanced Technolgy, Kainan, JAPAN, 3University of Pennsylvania, Philadelphia, PA, 4Department of Chemical and Biomolecular Engineering, University of Maryland, College Park, MD, 5Department of Chemistry, University of Maryland, College Park, MD, 6Georgia State University, Atlanta, GA, 7Institute of Biomedical Informatics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, 8Biomedical Sciences, University of Pennsylvania, Philadelphia, PA.
2:05 pm SG96 The Actg1 Nucleotide Sequence is Required for Normal Mouse Survival. L. J. Sundby1, W. M. Southern2, K. M. Hawbaker3, J. M. Trujillo2, B. J. Perrin3, J. M. Ervasti2; 1Molecular, Cellular, Developmental Biology, and Genetics, University of Minnesota, Minneapolis, MN, 2Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Minneapolis, MN, 3Biology, Indiana University–Purdue University Indianapolis, Indianapolis, IN.
2:20 pm Break.
2:35 pm SG97 Lateral interactions between beta-tubulins determine the temperature dependence of microtubule dynamics. G. Li, J. Moore; Department of Cell and Developmental Biology, University of Colorado School of Medicine, Aurora, CO.
2:50 pm SG98 Tubulin isotypes are optimized for distinct spindle function mechanisms during mitosis in budding yeast. E. Nsamba1, A. Bera1, M. Costanzo2, C. Boone2, M. Gupta1; 1Iowa State University, Ames, IA, 2University of Toronto, Toronto, ON, CANADA.
3:05 pm SG99 Developing a Systematic Screen of Reader Proteins for Tubulin Tyrosination-Detyrosination Cycle. T. Hotta1, S. Haynes2, A. Nesvizhskii2,3, D. Sept4, R. Ohi1; 1Cell and Developmental Biology, University of Michigan, Ann Arbor, MI, 2Pathology, University of Michigan, Ann Arbor, MI, 3Computational Medicine and Bioinformatics, University of Michigan, Ann Arbor, MI, 4Biomedical Engineering, University of Michigan, Ann Arbor, MI.
3:20 pm Break.
3:35 pm SG100 Setdb1 regulates microtubule dynamics. G. Gerlitz, R. Hernandez-Vicens, N. Pernicone, T. Listovsky; Molecular Biology, Ariel University, Ariel, ISRAEL.
3:36 pm SG101 Direct Talin-Actin Binding is Required for Mammalian Development. W. Deng1, A. Haage2, R. Carr1, G. Tanentzapf1; 1University of British Columbia, Vancouver, BC, CANADA, 2University of North Dakota, Grand Forks, ND.
3:37 pm SG102 Ssna1 stabilizes dynamic microtubules and detects microtubule damage. E. J. Lawrence, C. Arnaiz, G. Arpag, M. Zanic; Cell and Developmental Biology, Vanderbilt University, Nashville, TN.
3:38 pm SG103 Septins are Necessary for Detachment and Protrusion Formation in Border Cell Migration. A. M. Gabbert, J. A. Mondo, J. P. Campanale, D. J. Montell; MCDB, University of California, Santa Barbara, Santa Barbara, CA.
3:39 pm SG104 Integrated cell and in situ structural biology of Arp2/3 Complex Isoform-regulated Cell Motility and Lamellipodium Architecture. F. Fäßler, M. G. Javoor, F. W. Hofer, J. Stanger, G. Dimchev, V. Hodirnau, F. K. M. Schur; Institute of Science and Technology (IST) Austria, Klosterneuburg, AUSTRIA.
3:40 pm SG105 Phosphoregulation of y-Tubulin Directs Spindle Assembly Through Kinesin-5 and Microtubule Number. S. Sim, K. Morelli, A. Zhao, J. Vogel; Biology, McGill University, Montreal, QC, CANADA.
3:41 pm SG106 Myogenin controls non-centrosomal microtubule organizing center formation at the nuclear envelope. R. Becker1, S. Vergarajauregui1, F. Billing1, M. Sharkova1, E. Lippolis2, K. Mamchaoui3, F. Ferrazzi1, F. B. Engel1; 1Department of Nephropathology, Institute of Pathology, FAU Erlangen-Nürnberg, Erlangen, GERMANY, 2Institute of Human Genetics, FAU Erlangen-Nürnberg, Erlangen, GERMANY, 3Center for Research in Myology, Sorbonne Universités UPMC Université Paris 06, Paris, GERMANY.
3:42 pm SG107 Precise control of microtubule disassembly in living cells. G. Liu, S. Chen, K. Shaiv, S. Hong, W. Yang, S. Huang, Y. Chang, H. Cheng, Y. Lin; Institution of Molecular Medicine, National Tsing Hua University, HsinChu City, TAIWAN.
3:43 pm SG108 Cryo-EM structures illuminate the mechanisms of Arp2/3 complex activation and inhibition. R. Dominguez1, M. Boczkowska1, G. Rebowski1, F. E. Fregoso1, G. Simanov2, A. M. Gautreau2; 1University of Pennsylvania, Philadelphia, PA, 2Ecole Polytechnique, Paris, FRANCE.
3:58 pm SG109 Evolutionary diversification of Drosophila Arp2 for specialized actin branching. C. M. Schroeder1, S. A. Tomlin2, H. S. Malik2; 1UT Southwestern Medical Center, Dallas, TX, 2Howard Hughes Medical Institute, Fred Hutchinson Cancer Research Center, Seattle, WA.
Scientific Tracks: Physical Cell, Specialized Cell & Evolution
Organizers: Janis Burkhardt, University of Pennsylvania, Morgan Huse, Memorial Sloan-Kettering Cancer Center
Structural plasticity is a defining property of immune cells, which adopt distinct architectural configurations for vascular transport, interstitial migration, phagocytic uptake, and intercellular communication. Importantly, the capacity for shape change allows immune cells to exert nanonewton-scale forces against the extracellular matrix (ECM) and the surfaces of other cells. In recent years, it has become clear that this mechanical output is not only a prerequisite for motility, but also a means by which immune cells exchange information with their environment. Force coupling to mechanosensitive cell surface receptors enables immune cells to measure and respond to the biophysical properties of opposing surfaces. Applied force also functions as an avenue for signaling in its own right by modulating the strength and specificity of immune synapses, the cell-cell interactions that control intercellular communication and effector function in the immune system. This Special Interest Subgroup will highlight recent developments in mechanoimmunology, a new field that has emerged from the intersection of cell biology, biophysics, and immunology. Talks will cover the molecular mechanisms that govern mechanical activity in immune cells and also how these mechanisms contribute to immune function and dysfunction, both in vitro and in vivo. In this manner, we hope to place the immune response in a mechanical context, laying a foundation for future interdisciplinary work in this area.
1:45 pm Introduction.
1:50 pm SG110 Mechanical Control of Cytotoxic Secretion. M. Huse; Memorial Sloan Kettering Cancer Center, New York, NY.
2:05 pm SG111 Dynamic interactions of the actin and microtubule cytoskeleton during T cell activation and cytolytic function. A. Upadhyaya; Physics, University of Maryland, College Park, MD.
2:20 pm SG112 Cooperative ectodomain interaction among TCRαβ, CD3γε and CD3δε. Z. Yuan1, C. Ge1, A. Natarajan2, P. Cong1, S. Travaglno1, S. Beesam2, D. Grazette2, M. Krogsgaard2, C. Zhu1; 1Georgia Inst Technol, Atlanta, GA, 2New York University School of Medicine, New York, NY.
2:35 pm SG113 Dynamics of B cell synapse formation on viscous substrates. K. M. Spillane; Department of Physics and Randall Centre for Cell & Molecular Biophysics, King's College London, London, UNITED KINGDOM.
2:50 pm SG114 Exerting and sensing forces during integrin-mediated phagocytosis. V. Jaumouillé; Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, BC, CANADA.
3:05 pm SG115 Phosphatidylserine-mediated phagocytosis involves coreceptors TREM2, CD14 and integrin αMβ2 and a sinking engulfment mechanism. D. Vorselen1, R. A. Kamber2, R. D. Labitigan1,3, M. Delgado1, M. C. Bassik2, J. A. Theriot1,4; 1Dept. of Biology, University of Washington, Seattle, WA, 2Dept. of Genetics, Stanford University, Stanford, CA, 3Dept. of Biochemistry, Stanford University, Stanford, CA, 4Howard Hughes Medical Institute, University of Washington, Seattle, WA.
3:20 pm Break.
3:30 pm SG116 Invasion and locomotion of immune cells in dense environments. M. Sixt; IST Austria, IST Austria, Klosterneuburg, AUSTRIA.
3:45 pm SG117 The BAR domain protein SH3BP1 regulates plasma membrane tension to control immune cell migration. E. Sitarska1, J. Stopp2, M. Sixt2, A. Diz-Muñoz1; 1EMBL, Heidelberg, GERMANY, 2IST Austria, Klosterneuburg, AUSTRIA.
4:00 pm SG118 Impact of Mechanical stress on dendritic cell migration and fate. Z. Alraies, G. Nader, D. Sanséau, G. Delgado, M. Piel, A. Lennon-Dumenil; Institut Curie, Paris, FRANCE.
Scientific Tracks: Signaling and Metabolism, Cells in Distress & Disease
Organizers: Cole Haynes, University of Massachusetts Medical School , Agnel Sfeir, Sloane Kettering Institute/MSKCC
Mitochondria are key organelles with essential roles in metabolism, innate immunity, and regulated cell death. The mitochondrial proteome is comprised of proteins encoded by the nuclear genome as well as mitochondrial genomes. Thus, crosstalk between both genome-containing organelles is required for efficient mitochondrial biogenesis and to ensure cellular homeostasis. In recent years, exciting studies unveiled numerous signaling networks that coordinate the functions of the nucleus and mitochondria. In this session we feature a variety of such mito-nuclear communication networks, including mechanisms driven by secondary messengers and ones shaped by genetic interactions.
1:45 pm SG119 The mitochondrial outer membrane carrier MTCH2 regulates mitochondrial fusion in response to lipogenesis. J. Nunnari, K. Labbe; Department of Molecular and Cellular Biology, University of California, Davis, Davis, CA.
2:05 pm SG120 Mitochondrial stress is relayed to the cytosol by an OMA1-DELE1-HRI pathway. X. Guo1, G. Aviles1, R. Tian2, Y. Liu1, B. A. Unger3, Y. T. Lin4, A. P. Wiita1, K. Xu3, A. Correia1, M. Kampmann1; 1University of California, San Francisco, San Francisco, CA, 2SUSTech, Shenzhen, CHINA, 3University of California, Berkeley, Berkeley, CA, 4University of Texas Health San Antonio, San Antonio, TX.
2:20 pm SG121 Nuclear-based quality control of non-imported mitochondrial proteins. V. P. S. Shakya, W. A. Barbeau, T. Xiao, C. S. Knutson, M. Schuler, A. L. Hughes; Department of Biochemistry, University of Utah, Salt Lake City, UT.
2:35 pm SG122 A developmental mitophagy resets the mitochondrial proteome to facilitate germline mtDNA quality control. J. M. Palozzi, S. P. Jeedigunta, A. V. Minenkova, V. Montiero, Z. Thompson, T. R. Hurd; Molecular Genetics, University of Toronto, Toronto, ON, CANADA.
2:50 pm Break.
2:55 pm SG123 Mitochondrial Stress Induced Activation of Nuclear Encoded Innate Immune Genes. R. Youle; National Institute of Neurological Disorders and Strokes, Bethesda, MD.
3:15 pm SG124 Mitochondrial DNA replication stress activates mitochondrial retrograde signaling via a novel mtDNA-release pathway. L. E. Newman, N. Tadepalle, C. R. Schiavon, G. R. Rojas, U. Manor, G. S. Shadel; Molecular Cell Biology Laboratory, Salk Institute for Biological Studies, La Jolla, CA.
3:30 pm SG125 Systematic analysis of human mitochondrial translatomes reveals points of mitonuclear balance. I. Soto1, M. Couvillion1, E. McShane1, K. Hansen1, J. C. Moran2, A. Barrientos2, L. S. Churchman1; 1Genetics, Harvard Medical School, Boston, MA, 2Neurology, University of Miami Miller School of Medicine, Miami, FL.
3:45 pm SG126 A tRNA processing enzyme is a central regulator of the mitochondrial unfolded protein response. J. Held, B. Saunders, C. Pereira, M. Patel; Vanderbilt University, Nashville, TN.
4:00 pm SG127 Mapping the genetic landscape of complex cellular phenotypes with genome-scale Perturb-seq. J. M. Replogle1, R. Saunders1, A. Pogson1, T. Norman2, J. S. Weissman1; 1Whitehead Institute, Cambridge, MA, 2Memorial Sloan Kettering Cancer Center, New York, NY.
Scientific Track: Physical Cell
Organizers: Clarice Aiello, University of California, Los Angeles, Wendy Beane, Western Michigan University, Youngchan Kim, University of Surrey
Quantum Biology is an emergent interdisciplinary research field grounded on recent findings in physics, biology and engineering that suggest that quantum phenomena play a relevant role in shaping biology. Such quantum mechanical effects might influence physiological processes such as animal navigation, metabolic regulation in cells, optimal energy harvesting in photosynthesis, and quantum tunnelling in enzymes and DNA mutation. This first-of-a-kind session aims to introduce the field to the ASCB members, with views to engage the community and shape collaborations. Successful efforts in Quantum Biology are contingent on interdisciplinarity, multi-scale approaches, and close theory-experiment collaboration which can only be met within a strong research network.
1:45 pm SG128 Radical pair based magnetic field effects on cellular photochemistry. N. Ikeya, J. R. Woodward; Graduate School of Arts and Sciences, The University of Tokyo, Tokyo, JAPAN.
2:15 pm SG129 Cryptochrome - A Photoreceptor that Responds to Electromagnetic Fields. M. Ahmad1, M. Pooam1, B. Aguida1, N. Jourdan1, O. Ouari2, H. Karoui2; 1Sorbonne Université, Paris, FRANCE, 2Aix-Marseille Universite, Amiens, FRANCE.
2:45 pm SG130 Excited Electrons in Human Disease. D. E. Brash; Therapeutic Radiology, Yale University School of Medicine, New Haven, CT.
3:15 pm SG131 Using biological inspiration for chemical physics informed design of chromophores for shortwave infrared imaging and quantum measurement. J. R. Caram; University of California, Los Angeles, Los Angeles, CA.
3:45 pm SG132 Using QuantumPhenomena to Control ROS-Mediated Stem Cell Proliferation. W. S. Beane; Western Michigan University, Kalamazoo, MI.
Scientific Tracks: Cellular Dynamics, Cells in Distress & Disease
Organizers: Joana Ferreira, University of Oxford, Prasanna Satpute-Krishnan, UniforMedical Services University, Guillaume Thibault, Nanyang Technological University, Haoxi Wu, University of Colorado Boulder
The endoplasmic reticulum (ER) is the hub for lipid and secretory pathway protein biosynthesis. The ER plays a central role in maintaining most compartments of the endomembrane system through vesicular transport or contact sites. Within the ER, lipid and protein dynamics are tightly coupled in many functions including (i) lipid droplet biogenesis, (ii) lipid and protein sorting in the secretory pathway, (iii) protein quality control and cell stress responses, (iv) and lipid transfer at contact sites. This subgroup will bring together scientists who study how the interplay of proteins and lipids in the ER impacts global cellular physiology.
1:45 pm Introduction.
1:50 pm SG133 Endoplasmic reticulum stress sensor ire-1 is detrimental in young but essential in aged animals fed high glucose diet. G. Thibault; School of Biological Sciences, Nanyang Technological University, Singapore, SINGAPORE.
2:10 pm SG134 Rhomboid pseudoproteases employ lipid distortion function for retrotranslocating ERAD membrane substrates. S. Neal; University of California, San Diego, La Jolla, CA.
2:30 pm SG135 Order from disorder: COPII coat assembly driven by intrinsically disordered domains. E. Miller, X. Li, V. Stancheva; MRC Laboratory of Molecular Biology, Cambridge, UNITED KINGDOM.
2:50 pm SG136 Apoe at the intersection of lipid storage and secretion. S. Cohen, I. Windham; UNC, Chapel Hill, NC.
3:10 pm SG137 Multimodality structured illumination microscopy for super-resolution live-cell imaging. D. Li; Institute of Biophysics Chinese Academy of Science, Beijing, CHINA.
3:30 pm SG138 Pex30-like proteins function as adaptors at distinct endoplasmic reticulum membrane contact sites. J. P. Verissimo Ferreira, P. Carvalho; University of Oxford, Oxford, UNITED KINGDOM.
3:50 pm SG139 Spartin is a receptor mediating the selective autophagy of lipid droplets. J. Chung1,2, J. Park3, Z. Lai1,2, R. C. Richards1,2, R. V. Farese Jr.1,2, T. C. Walther1,2; 1Department of Cell Biology, Harvard Medical School, Boston, MA, 2Department of Molecular Metabolism, Harvard T. H. Chan School of Public Health, Boston, MA, 3Department of Pharmacology, Department of Neurology, Wayne State University, Detroit, MI.
Wednesday, December 8 | 1:45 PM - 4:15 PM EST
Scientific Tracks: Cellular Genome, Cellular Dynamics
Organizers: Fei Li, Department of Biology, New York University, Barbara Mellone, Molecular and Cell Biology &
Institute for Systems Genomics, University of Connecticut
Centromeres guide the assembly of kinetochores to ensure proper segregation of chromosomes during mitosis and meiosis. Despite their universally conserved function, centromeres vary in their organization, DNA sequence, and associated proteins. Dysfunctional centromeres have long been known to be associated with diseases, including cancer. The study of centromeres has led to major leaps in our understanding of chromosome segregation, epigenetic mechanisms, cell cycle regulation, chromosome evolution, and disease. The centromere field recently has made rapid progress by exploiting the latest technical advances including super-resolution imaging and long-read DNA sequencing. These new technologies enable groundbreaking discoveries in centromere biology and pave the way towards potential novel therapies for diseases resulting from mis-regulation of centromeres. This subgroup will bring together researchers across the globe and spanning career stages to present and discuss the latest exciting findings on the fundamentals of centromere biology as well as new concepts within the field, ranging from centromere inheritance to centromere evolution. We will approach these themes in a variety of model organisms. The role of centromeres in physiology and disease will also be highlighted.
1:45 pm SG140 DNA repair proteins moonlighting at the inner centromere. K. Bloom, J. Stanton, D. Cook, J. Lawrimore, E. Yeh; University of North Carolina, Chapel Hill, NC.
1:59 pm SG141 The kinetochore protein KKT23 acetylates histone H2A to promote chromosome segregation in kinetoplastids. P. Ludzia, M. Ishii, B. Akiyoshi; Biochemistry, University of Oxford, Oxford, UNITED KINGDOM.
2:10 pm SG142 Human centromeres drift through cellular proliferation. Y. Nechemia-Arbely1, M. A. Mahlke1, L. Lumerman1, P. Nath1, R. Raphael1, P. Ly2; 1University of Pittsburgh, UPMC Hillman Cancer Center, Pittsburgh, PA, 2University of Texas Southwestern Medical Center, Dallas, TX.
2:24 pm SG143 Centromere Function in the Asymmetric Cell Division of Drosophila Germline Stem Cells. A. Kochendoerfer, E. Dunleavy; National University of Ireland Galway, Galway, IRELAND.
2:38 pm SG144 Structural consequences and dynamics of pericentric satellite expression. D. M. Carone; Swarthmore College, Swarthmore, PA.
2:52 pm SG145 A DNA damage-independent and ATR-regulated pathway protects CENP-A occupancy at interphase centromeres. I. Trier1,2, Z. Toksoy1, L. Kabeche1,2; 1Molecular Biophysics & Biochemistry, Yale University, New Haven, CT, 2Yale Cancer Biology Institute, New Haven, CT.
3:03 pm Break
3:08 pm SG146 A dynamic SUMO cycle ensures stable centromeric chromatin inheritance. S. Mitra, S. van den Berg, S. East, I. Abdul Zani, L. E. T. Jansen; Department of Biochemisrty, University of Oxford, Oxford, UNITED KINGDOM.
3:22 pm SG147 Aurora B activation by phosphorylation is a synergistic process inducing structural organization and synchronized internal motion of the enzyme. D. Segura-Peña1, O. Hovet1, J. M. Dawicki-McKenna2, S. M. Hansen Wøien1, B. E. Black2, M. Cascella1, N. Sekulić1; 1University of Oslo, Oslo, NORWAY, 2University of Pennsylvania, Philadelphia, PA.
3:33 pm SG148 Mislocalization of CENP-A contributes to chromosomal instability (CIN) and anueuploidy. M. A. Basrai; Genetics, National Cancer Institute, NIH, Bethesda, MD.
3:47 pm SG149 Molecular Dissection of the KMN network on the CENP-T pathway. T. Fukagawa; FBS, Osaka University, Osaka, JAPAN.
4:01 pm SG150 Modulating and Rewiring Cell Division. I. Cheeseman; Department of Biology, MIT, Whitehead Institute, Cambridge, MA.
Scientific Tracks: Cells in Distress & Disease, Cellular Dynamics
Organizers: Jessica Henty-Ridilla, SUNY Upstate Medical University, Emily Mace, Columbia University, Patrick Oakes, Loyola University
The cytoskeleton lies at the heart of many of the most fundamental cellular functions where it acts as a scaffold for innumerable biochemical and mechanical signaling pathways. While many of these essential processes and their component molecules have been defined in great detail, we often still do not understand how they sum together to dictate healthy versus disease states downstream. From the molecular to the tissue scale we will explore the implications of when these processes work and when they go awry, focusing on the roles that the cytoskeleton and its components play in human health. We aim to bring together a wide array of presenters from different perspectives, backgrounds, and career stages to address this common unifying theme.
1:45 pm SG151 Membrane and Cytoskeletal Dynamics During Muscle Progenitor Migration, Proliferation and Differentiation. R. C. Adikes1, T. Gibney2, O. B. Ahmed3, T. L. McKnight1, A. Fang3, W. Zhang3, B. Goldstein4, A. Pani2, D. Q. Matus3; 1Department of Biology, Siena College, Lodonville, NY, 2Department of Biology, University of Virginia, Charlottesville, VA, 3Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, NY, 4Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC.
2:00 pm SG152 Towards a mechanistic understanding of motile and primary cilia with CLEM and cryo-electron tomography. G. Pigino1,2; 1Human Technopole, Milan, ITALY, 2Cbg, Max Planck Institute, Dresden, GERMANY.
2:20 pm SG153 Coordination of alpha-tubulin acetylation and mitochondrial transport by MFN2. A. Kumar1, D. Larrea2, M. Pero1, P. Infante3, G. Shin1, L. Di Marcotullio3, E. Area-Gomez2, F. Bartolini1; 1Pathology & Cell Biology, Columbia University, New York, NY, 2Neurology, Columbia University, New York, NY, 3University of Rome "La Sapienza", Rome, ITALY.
2:35 pm SG154 The integrin-actin linker Tensin1 contributes to focal adhesion disassembly at mitosis to relieve an integrin-inactivation G2-M checkpoint. H. R. Thiam1, A. M. Pasapera1, E. Degaga2, J. S. Urbach2, C. M. Waterman1; 1Cell and Developmental Biology Center, National Heart, Lung, and Blood Institute, NIH, Bethesda, MD, 2Department of Physics and The Institute for Soft Matter Synthesis and Metrology, Georgetown University, Washington, DC.
2:55 pm Break.
3:05 pm SG155 Trim9 and VASP ubiquitination regulate actin dynamics and neuronal morphology. L. E. McCormick1, L. Herring2, G. Diering1, N. G. Brown3, S. L. Gupton1; 1Cell Biology and Physiology, University of North Carolina At Chapel Hill, Chapel Hill, NC, 2Michael Hooker Proteomics Core Facility, University of North Carolina At Chapel Hill, Chapel Hill, NC, 3Pharmacology, University of North Carolina At Chapel Hill, Chapel Hill, NC.
3:20 pm SG156 Untangling the interdependent actin filament nucleation and elongation activities of formin. N. Courtemanche; University of Minnesota, Minneapolis, MN.
3:40 pm SG157 ALS-linked PFN1 mutants cause mitochondria defects through loss and gain of function. T. A. Read1, K. Skruber2, B. A. Cisterna1, E. A. Vitriol1; 1Department of Neuroscience and Regenerative Medicine, Medical College of Georgia at Augusta University, Augusta, GA, 2Department of Cellular and Molecular Pharmacology, University of California San Francisco, San Francisco, CA.
3:55 pm SG158 Forces and integrin activity in cancer cell migration. j. Ivaska; University of Turku, Turku, FINLAND.
Scientific Tracks: Specialized Cell & Evolution and Signaling & Metabolism
Organizers: Meghan Morrissey, University of California Santa Barbara, Xaolei Su, Yale University, Marcus Taylor, Max Planck Institute for Infection Biology
The immune system faces an extreme version of the problems that all signaling systems must face: how to discriminate between closely related signals and activate the appropriate pathway. This subgroup will bring together a cross-disciplinary group of researchers investigating how immune systems recognize and respond to threats. We will also explore how this knowledge informs cancer immunotherapy. The diverse topics and tools covered by our speakers will emphasize the breadth of this growing field, and encourage interdisciplinary research.
1:45 pm Introduction.
1:50 pm SG159 Size-dependent activation of chimeric antigen receptor. Q. Xiao, X. Su; Yale Univ., New Haven, CT.
2:05 pm SG160 Novel mechanisms controlling secretion from immune cells. G. Griffiths; University of Cambridge, Cambridge, UNITED KINGDOM.
2:20 pm SG161 Nano-optogenetic immunotherapy. Y. ZHOU; Institute of Biosciences and Technology, Texas A&M University, HOUSTON, TX.
2:35 pm Break 1.
2:40 pm SG162 Mucins form a nanoscale physical barrier against Natural Killer cell cytotoxicity. S. Park1, M. Colville1,2, C. Shurer2, J. Kuo2, J. Paek3, L. Huang2, J. Su4, W. Zipfel1,3, C. Fischbach3, H. Reesink4, M. Paszek1,2,3; 1Biophysics, Cornell University, Ithaca, NY, 2Robert Frederick Smith Schoold of Chemical and Biomolecular Engineering, Cornell University, Ithaca, NY, 3Nancy E. and Peter C. Meing School of Biomedical Engineering, Cornell University, Ithaca, NY, 4Department of Clinical Science, College of Veterinary Medicine, Cornell University, Ithaca, NY.
2:55 pm SG163 Competition between activation and inhibition in macrophage phagocytosis. D. Fletcher; University of California, Berkeley, Berkeley, CA.
3:10 pm SG164 Myd88 oligomer size functions as a physical threshold to trigger IL1R Myddosome signaling. R. Deliz-Aguirre; Visualization of Immune Signaling, Max Planck Institute for Infection Biology, Berlin, GERMANY.
3:25 pm Break 2.
3:30 pm SG165 Bioengineering approaches to study the phagosome. B. D. Bryson; Massachusetts Institute of Technology, Cambridge, MA.
3:45 pm SG166 Deep-sea microbes as tools to refine the rules of innate immune pattern recognition. A. Gauthier; Harvard University, Boston, MA.
4:00 pm SG167 Sting mediates immune responses in the closest living relatives of animals. A. Woznica; UT Southwestern Medical Center, Dallas, TX.
Scientific Tracks: Physical Cell, Signaling & Metabolism
Organizers: Meghan Driscoll, University of Texas Southwestern Medical Center, Assaf Zaritsky, Ben-Gurion University
Deciphering cellular architecture is fundamental to cell biology since protein spatial patterning and interactions are key readouts of cell function in health and disease. Although individual labs can focus only on limited numbers of proteins, larger organizations have recently embraced the challenge of generating large scale and comprehensive datasets, as well as computational tools that enable data visualization and analysis. Now is the time for the cell biology community to come together to plan how to transform these rich and comprehensive datasets into fundamental discoveries. In this subgroup, we will bring atlas creators together with other cell biologists and data scientists to ask what makes a resource useful, how scientists from diverse organizations can utilize these resources, and how these resources can be integrated to further drive our understanding of the basic principles of cell organization and function.
1:45 pm Introduction.
1:50 pm SG168 Whole cell organelle segmentation in volume electron microscopy. A. Weigel; Janelia Research Campus, Ashburn, VA.
2:05 pm SG169 Robust integrated intracellular organization of the human iPS cell: where, how much, and how variable. M. P. Viana1, . Allen Institute for Cell Science1, J. A. Theriot2, S. M. Rafelski1; 1Allen Institute for Cell Science, Seattle, WA, 2Department of Biology and Howard Hughes Medical Institute, University of Washington, Seattle, WA.
2:20 pm SG170 Jump-cell painting - powering drug discovery and development with images. S. Garg; Merck Healthcare KGaA, Darmstadt, GERMANY.
2:35 pm SG171 Image-based spatiotemporal dissection of the human proteome. E. Lundberg; KTH Royal Institute of Technology, Stockholm, SWEDEN.
2:50 pm Break.
3:00 pm SG172 Opencell and cytoself: integrating genome engineering, live imaging, proteomics and deep learning for the cartography of human cellular architecture. M. D. Leonetti, L. A. Royer; Quantitative Cell Science, Chan Zuckerberg Biohub, San Francisco, CA.
3:20 pm SG173 Machine learning methods for the integration of spatio-temporal microscopy data with transcriptomics data. P. Villoutreix; LIS (UMR 7020), IBDM (UMR 7288), Turing Center For Living Systems, Aix-Marseille University, Marseille, FRANCE.
3:35 pm Panel Discussion. Panelists are: Quincey Justman, Cell Systems; Manuel Leonetti, Chan Zuckerberg Biohub; Jennifer Lippincott-Schwartz, Janelia Research Campus; Loic Royer, Chan Zuckerberg Biohub; and Jason Swedlow, Wellcome Leap and University of Dundee
Scientific Tracks: Physical Cell, Communal Cell
Organizers: Ryan Petrie, Drexel University, Bojana Gligorijevic, Temple University
The growing focus on how mechanical forces govern cell-matrix interactions and tissue dynamics highlights the enormous overlap between the general fields of biomedical engineering, biophysics, and cell biology. For example, investigating the role of hydraulic pressure in cell and tissue function has revealed a profound connection between this mechanical force in mammalian blastocysts controlling shape and cell differentiation, and in single cells, where pressure is intimately linked to the types of protrusions the cells use to navigate the tights spaces in 3D matrix. Further, the three-dimensional architecture of the extracellular matrix can increase the force applied to the cell, which in turn can affect remodeling of the matrix by cancer cells, as well as the susceptibility of nuclear DNA to damage. This session will highlight the most recent advances in our understanding of the connections between physical forces and cell biology and how they govern dynamic cell behavior and tissue organization. It will also be of immediate interest to those just beginning to consider the role of mechanical forces in their own cell biology research.
1:45 pm Introduction.
1:46 pm SG174 STReTCh: a strategy for detection of mechanical forces across proteins in cells. B. L. Zhong, V. T. Vachharajani, A. R. Dunn; Stanford University, Stanford, CA.
1:53 pm SG175 Nuclear and chromatin mechanics in cell state regulation Mechanical forces and the nucleus: regulation of cell faCellte asdfdsfnd integritynd integrity. Y. Miroshnikova; Laboratory of Molecular Biology NIDDK, National Institutes of Health, Bethesda, MD.
2:10 pm SG176 The Ras-MAPK pathway senses 3D matrix structure to regulate hydraulic pressure and the mode of cell migration. T. M. Jones, D. McKeon, R. J. Petrie; Biology, Drexel University, Philadelphia, PA.
2:27 pm SG177 Mechanical worrying drives cell migration in crowded environments. M. K. Driscoll1, E. Welf1, E. Sapoznik1, V. Murali1, A. Weems1, J. Garcia-Arcos2, M. Roh-Johnson3, K. M. Dean1, M. Piel2, R. Fiolka1, G. Danuser1; 1UT Southwestern Medical Center, Dallas, TX, 2Institut Curie, Paris, FRANCE, 3University of Utah, Salt Lake City, UT.
2:34 pm SG178 Invadopodia enable cooperative invasion and metastasis of breast cancer cells. L. Perrin1, B. Bayarmagnai2, E. Tuzel2, B. Gligorijevic2,3; 1Institut Curie, Paris, FRANCE, 2Temple University, Philadelphia, PA, 3Fox Chase Cancer Center, Philadelphia, PA.
2:51 pm Break.
2:56 pm SG179 A tumor-derived type III collagen-rich ECM niche regulates tumor cell dormancy. J. S. Di Martino1, A. Nobre1, C. Mondal1, I. Taha2, E. Farias1, E. Fertig3, A. Naba2, J. Aguirre-Ghiso1, J. Bravo-Cordero1; 1Hematology Oncology, Icahn school of medicine at Mount Sinai, NEW YORK, NY, 2Department of Physiology and Biophysics, University of Illinois at Chicago, chicago, IL, 3Departments of Oncology, Applied Mathematics and Statistics, and Biomedical Engineering, Johns Hopkins University, BALTIMORE, MD.
3:03 pm SG180 Cancer associated fibroblasts actively compress cancer cells and modulate mechanotransduction. J. Barbazan1, C. Perez-Gonzalez2, M. Gomez-Gonzalez3, M. Dedenon2, S. Richon2, E. Latorre3, M. Serra2, P. Mariani2, S. Descroix2, P. Sens2, X. Trepat3, D. Matic-Vignjevic2; 1Health Research Institute of Santiago de Compostela, Santiago de Compostela, SPAIN, 2Institut Curie, Paris, FRANCE, 3Institute of Bioengineering of Catalonia (IBEC), Barcelona, SPAIN.
3:10 pm SG181 Fibrillar collagen in tumor microenvironment determines the mode of tumor progression. W. Jung, J. Flournoy, Y. Chen; Mechanical Engineering, Johns Hopkins University, Baltimore, MD.
3:27 pm SG182 Exploring cell mechanisms of tissue fluidity by optogenetic manipulation of Rho activity. M. Herrera-Perez, C. Cupo, K. Kasza; Columbia University, New York, NY.
3:34 pm SG183 Tissue hydraulics in early mammalian development. C. Chan1, M. Costanzo2, T. Ruiz-Herrero3, G. Mönke2, R. Petrie4, M. Bergert2, A. Diz-Muñoz2, L. Mahadevan5, T. Hiiragi2; 1Mechanobiology Institute, National University of Singapore, Singapore, SINGAPORE, 2EMBL Heidelberg, Heidelberg, GERMANY, 3Paulson School of Engineering and Applied Sciences, Cambridge, MA, 4Drexel University, Philadelphia, PA, 5Harvard University, Cambridge, MA.
3:51 pm SG184 A tubule tension switch regulates the physical packing geometry of epithelial branch tips in the developing kidney. L. S. Prahl, J. M. Viola, A. Gupta, A. J. Hughes; Bioengineering, University of Pennsylvania, Philadelphia, PA.
3:58 pm SG185 Pressure and curvature control of contact inhibition in epithelia growing under spherical confinement. I. Di Meglio, A. Trushko, P.
Scientific Tracks: Cellular Dynamics, Physical Cell
Organizers: Gautam Dey, EMBL, Padmini Rangamani, University of California San Diego, Ishier Raote, CRG, Andela Saric, University College London
A small number of protein families, many traceable through 2 billion years of evolutionary time to the common ancestors of all eukaryotes, together shape lipid membranes into the breathtaking diversity of dynamic compartments and structures that define eukaryotic cells. Biochemistry and structural studies reveal the physicochemical basis for the ability of proteins to shape, bend, and remodel membranes; mechanical modeling shows that stresses acting on the membrane can be common factors across different curvature- generating mechanisms; phylogenetics links orthologs across evolutionary time; finally, genetics and microscopy anchor this understanding within a sub-cellular and organismal context.
Building on a successful event from CellBio2020 that was described by participants as "an excellent session with much diversity of science and approaches!", we will once again bring together leaders in biochemistry, evolutionary biology and cell biology, linking these disparate strands of research under the umbrella of “evolutionary biochemistry” (Harms, Thornton 2013). We expect that this holistic perspective will lead to a better understanding of how and why biological molecules have the properties that they do - shedding light on the key membrane remodelling processes that power cellular life.
1:45 pm Introduction.
1:48 pm SG186 Large self-assembled clathrin lattices spontaneously disassemble without sufficient adaptor proteins. S. Guo1, A. Sodt2, M. Johnson1; 1Biophysics, Johns Hopkins University, Baltimore, MD, 2National Institutes of Health, Bethesda, MD.
2:01 pm SG187 Adaptation of clathrin- and actin-mediated endocytosis in animal development. M. Mund1, T. Noordzij2, A. Picco1, M. Kaksonen1; 1University of Geneva, Geneva, SWITZERLAND, 2Cell Biology and Biophysics, European Molecular Biology Laboratory, Heidelberg, GERMANY.
2:14 pm SG188 Clathrin's Essential Role During Endocytosis is to Stabilize Cell Membrane Curvature. R. Cail, C. Shirazinejad, D. Drubin; University of California, Berkeley, Berkeley, CA.
2:27 pm SG189 Regulation of Clathrin-Mediated Endocytosis in the Context of Huntingtin Aggregates. D. Subramanyam; National Centre for Cell Science, Pune, INDIA.
2:40 pm SG190 Caveolae control intercellular tension during cytokinesis and promote successful abscission. V. Andrade1, J. Bai1, A. Jimenez2, N. Gupta-Rossi1, C. Delevoye2, C. Lamaze2, A. Echard1; 1Institut PASTEUR - CNRS, Paris, FRANCE, 2Institut CURIE - CNRS, Paris, FRANCE.
2:53 pm Break.
3:08 pm SG191 Theoretical modeling of membrane reshaping by membrane proteins. M. Arroyo, C. Tozzi, N. Walani; Universitat Politècnica De Catalunya-BarcelonaTech, Barcelona, SPAIN.
3:21 pm SG192 The ESCRT-III / PspA / Vipp1 superfamily of membrane remodelling proteins is conserved across the tree of life. D. Souza1, J. Liu2, M. Tassinari3, S. Naskar3, J. Noel4, O. Bohuszewicz3, M. Buck3, T. Williams5, B. Baum1, H. Low3; 1MRC Laboratory of Molecular Biology, Cambridge, UNITED KINGDOM, 2University of Oxford, Oxford, UNITED KINGDOM, 3Imperial College London, London, UNITED KINGDOM, 4Max Delbruck Center for Molecular Medicine, Berlin, GERMANY, 5University of Bristol, Bristol, UNITED KINGDOM.
3:34 pm SG193 Does membrane transformation point to hidden functions of active processes in bacteria?. P. Schwille; Max Planck Inst Biochem, Martinsried, GERMANY.
3:47 pm SG194 The herpesviral nuclear egress complex deforms and buds membranes by lipid ordering and protein scaffolding. M. K. Thorsen1, A. Lai2, M. W. Lee3, D. P. Hoogerheide4, G. C. L. Wong3, J. Freed2, E. E. Heldwein1; 1Tufts University, Boston, MA, 2Cornell University, Ithaca, NY, 3UCLA, Los Angeles, CA, 4National Institute of Standards and Technology, Gaithersburg, MD.
4:00 pm SG195 Intrinsically disordered protein networks drive membrane remodeling. J. Stachowiak; University of Texas At Austin, Austin, TX.
Scientific Tracks: Cellular Dynamics, Signaling & Metabolism
Organizers: Peter Bieling, MPI - Dortmund, Martin Loose, IST Austria
In vitro reconstitution of biological processes from their molecular components has emerged as a powerful and widely used tool in cell biology. Evolving from its roots in enzyme biochemistry, reconstitution of multicomponent cellular processes has revealed that fundamental cell functions and subcellular structures can be recapitulated at the mesoscale by combining component macromolecules with cell-like boundary conditions. These bottom-up approaches provide important mechanistic insight difficult to obtain through traditional top-down studies. Rapid progress in molecular self-assembly, micropatterning and fabrication, coupled with continued advancements in biochemistry and molecular biology, promise to yield more complete, systems reconstitutions that mirror the complex dynamics and organization of living cells. This session will feature a diverse set of researchers at the forefront of the most ambitious and pioneering examples of cellular reconstitution.
1:45 pm Introduction.
1:50 pm SG196 Structural Mechanism for Bi-Directional Actin Crosslinking by T-plastin. L. Mei1, M. J. Reynolds1, D. Garbett2, T. Meyer3, G. M. Alushin1; 1The Rockefeller University, New York, NY, 2Stanford University, Stanford, CA, 3Weill-Cornell Medical College, New York, NY.
2:08 pm SG197 Waves and spirals in contracting actomyosin networks. K. Keren1, A. Krishna1, M. Savinov2, N. Ierushalmi1, A. Mogilner2; 1Technion-Israel Institute of Technology, Haifa, ISRAEL, 2NYU, New York, NY.
2:26 pm SG198 Bottom-up reconstitution of focal adhesion complexes. N. Mizuno; National Institutes of Health, Bethesda, MD.
2:44 pm SG199 Mechanism of active dynein complex assembly. S. L. Reck-Peterson, E. Karasmanis, J. Reimer, S. Khuansuwan, A. Kendrick, A. E. Leschziner; Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA.
3:02 pm Break.
3:14 pm SG200 Dead-box atpases are global regulators of phase-separated organelles and rna flux. M. Hondele1, S. Heinrich2, J. Wang3, P. Vallotton2, B. M. A. Fontoura4, K. Weis2; 1University of Basel/Biozentrum, Basel, SWITZERLAND, 2ETH Zurich, Zurich, SWITZERLAND, 3Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, TX, 4Department of Cell Biology, University of Texas Southwestern Medical Center,, Dallas, TX.
3:32 pm SG201 Mechanism and function of T cell signaling Condensates. L. Zeng, X. Su; Yale University, New Haven, CT.
3:50 pm SG202 Force generation by protein-DNA co-condensation. T. Quail, S. Golfier, M. Elsner, K. Ishihara, V. Murugesan, R. Renger, F. Julicher, J. Brugues; Max Planck Institute, Dresden, GERMANY.
Friday, December 10 | 1:45 PM - 4:15 PM EST
Scientific Tracks: Specialized Cell & Evolution, Cellular Dynamics
Organizers: Damian Ekiert, NYU Grossman School of Medicine, Amilcar Perez, Johns Hopkins University
Prokaryotes thrive in a wide range of environments, from aquatic and saprophytic communities to our own microbiomes. In these complex environments, bacteria must compete for resources, grow and divide, communicate with neighboring cells, sense and respond to changes in the environment, and protect themselves from external attack. To these ends, bacteria have evolved fascinating and highly complex systems that mediate their interactions with other cells and the outside world. These include a complex cell envelope to resist mechanical forces and serve as a protective barrier; cytoskeletal proteins for cellular organization and division; signal transduction pathways for communication; and transport systems for import and export of biomolecules such as nutrients, proteins, and DNA. This subgroup will highlight the diversity of prokaryotic cell biology, with an emphasis on how the latest advances in quantitative biology and advanced imaging modalities (CryoEM/CryoET) are unravelling the organization and dynamics of the bacterial cell. This will be the third iteration of an exciting subgroup focused on prokaryotic cell biology.
1:45 pm Introduction.
1:45 pm SG211 Multiscale investigation of photo sensing in non-photosynthetic bacteria.. G.Chen, S. Mukherjee; University of Chicago, Chicago, IL.
2:00 pm SG212 Six ParAs, six cargos, one cell. L. Tran, A. G. Vecchiarelli; MCDB, University of Michigan, Ann Arbor, MI.
2:15 pm SG213 Inhibiting BamA to understand outer membrane protein folding. S. T. Rutherford; Genentech, Inc., South San Francisco, CA.
2:30 pm SG214 Hopanoid Lipids Regulate Membrane Fluidity in the Legume Root-Nodulating Bacterium Bradyrhizobium diazoefficiens. E. Lawrence, S. Talamantez-Lyburn, B. Belin; Embryology, Carnegie Institution for Science, Baltimore, MD.
2:35 pm SG215 A Framework to Assess Liquid-Liquid-Phase-Separation in Bacterial Cells. Y. Hoang, A. Vecchiarelli; University of Michigan, Ann Arbor, MI.
2:40 pm SG216 Pseudomonas aeruginosa directs twitching motility by mechanotaxis. M. J. Kühn1, L. Talà1, Y. Inclan2, R. Patino2, X. Pierrat1, I. Vos1,3, Z. Al-Mayyah1, H. MacMillan2, J. Negrete1,3, J. Engel2,4, A. Persat1; 1Institute of Bioengineering and Global Health Institute, Ecole Polytechnique Fédérale De Lausanne, Lausanne, SWITZERLAND, 2Department of Medicine, University of California, San Francisco, San Francisco, CA, 3Institute of Physics, Ecole Polytechnique Fédérale de Lausanne, Lausanne, SWITZERLAND, 4Department Microbiology and Immunology, University of California, San Francisco, San Francisco, CA.
2:55 pm Break.
3:05 pm SG217 Micro-crowdsourcing: how swarming bacteria integrate signals, recognize friends, and assemble communities . A. Chittor1, K. A. Gibbs2,1; 1Molecular and Cellular Biology, Harvard University, Cambridge, MA, 2University of California, Berkeley, Berkeley, CA.
3:20 pm SG218 Spatio-temporal control of DNA replication by the pneumococcal cell cycle regulator CcrZ. C. Gallay1, S. Sanselicio1, M. E. Anderson2, Y. Soh1, X. Liu1, G. A. Stamsås3, S. Pelliciari4, R. van Raaphorst1, J. Dénéréaz1, M. Kjos3, H. Murray4, S. Gruber1, A. D. Grossman2, J. Veening1; 1Department of Fundamental Microbiology, University of Lausanne, Lausanne, SWITZERLAND, 2Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, 3Faculty of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, Ås, NORWAY, 4Centre for Bacterial Cell Biology, Newcastle University, Newcastle Upon Tyne, UNITED KINGDOM.
3:35 pm SG219 Structural biology of prokaryotic cell surfaces. T. Bharat; Oxford University, Oxford, UNITED KINGDOM.
3:50 pm SG220 Many hands, light work: multiple operonic regulatory elements modulate transcription and translation of a cyanobacterial RNA helicase during cold stress. L. A. Brand, G. W. Owttrim; Biological Sciences, University of Alberta, Edmonton, AB, CANADA.
3:55 pm SG221 Intracellular Growth of L. monocytogenes is Linked to Bacterial Density Within Host Cell. P. Radhakrishnan1, F. Ortega2, J. Theriot3; 1Biophysics, Stanford University, Stanford, CA, 2Biochemistry, Stanford University, Stanford, CA, 3University of Washington, SEATTLE, WA.
4:00 pm SG222 Polyploidy and Organization of the Highly Segmented Genome of Borrelia burgdorferi, the Lyme Disease Spirochete. C. N. Takacs1, J. Wachter2, Y. Xiang3, M. Scott3, M. Stoner3, P. A. Rosa2, C. Jacobs-Wagner1; 1Biology, Stanford University, Stanford, CA, 2Bacteriology, NIH/NIAID, Hamilton, MT, 3Yale University, New Haven, CT.
Scientific Tracks: Signaling & Metabolism, Physical Cell
Organizers: Matt Good, University of Pennsylvania, Jan Skotheim, Stanford University
Proliferating eukaryotic cells coordinate growth, division, and differentiation processes. A broad variety of cell types, from yeast to human, contain a size checkpoint - a mechanism ensuring that they achieve a defined amount of growth or achieve a specific size in order to trigger the cell division cycle or differentiation. Recent studies have begun to uncover the molecular basis of cell size regulation both in cultured cells in vitro and in stem cells in vivo. An emerging phenomenon is that perturbations to cell size are sufficient to alter cell physiology, mechanics, and gene expression, and that evolution has leveraged changes in cell size to regulate events in the context of tissue biology and early embryo development.
This session will feature cutting-edge research on how cells sense and regulate their size and how this regulation contributes to cellular decision-making. We will highlight new concepts and techniques used to characterize cell size and growth regulation, as well as survey the latest insights on the mechanistic basis of cell size control. Additional topics may include the interdependence of cell growth, biosynthesis, and cell division that maintains protein, RNA, and organelle homeostasis. Moreover, we will discuss the dysregulation of cell size in disease, the role of cell size and shape in control of cell fate, and the size invariant scaling of signaling gradients in tissues and embryos.
1:45 pm Introduction.
1:47 pm SG223 Deep single-cell phenotyping uncovers general principles of cell size and cell cycle coordination in bacterial cells. S. Govers1, C. Jacobs-Wagner2; 1de Duve Institute, Brussels, BELGIUM, 2Biology, Stanford University/HHMI, Stanford, CA.
2:02 pm SG224 Cytoplasmic organization promotes protein diffusion. J. Ferrell1, W. Huang1, X. Cheng2; 1Stanford University, Stanford, CA, 2Biological Sciences, University of Southern California, Los Angeles, CA.
2:17 pm SG225 Size Scaling and Repurposing of Cell Division Machinery in Early Xenopus Embryos. C. M. Field, L. Bai, J. F. Pelletier, E. S. Van Italie, T. J. Mitchison; Systems Biology, Harvard Medical Sch, Boston, MA.
2:32 pm SG226 Mechanisms of mitotic chromosome scaling in Xenopus. C. Y. Zhou; Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA.
2:47 pm SG227 Coordination of Macromolecule Synthesis and Cell Volume. K. Marina1, A. Terhorst2, A. Amon3, G. Neurohr1; 1ETH Zurich, Zurich, SWITZERLAND, 2Massachusetts Institute of Technology, Zurich, SWITZERLAND, 3Massachusetts Institute of Technology, Cambridge, MA.
3:02 pm SG228 Nuclear growth and shape dynamics in growing hiPSCs. J. Cass1, . Allen Institute for Cell Science1, J. Theriot2, S. Rafelski1; 1Allen Institute for Cell Science, Seattle, WA, 2Biology and Howard Hughes Medical Institute, University of Washington, Seattle, WA.
3:17 pm SG229 Cytoskeletal and osmotic regulation of cell size. S. X. Sun, Q. Ni, Z. Ge, G. Fu; Johns Hopkins University, Baltimore, MD.
3:32 pm SG230 Cell size-dependent G1/S transition controls stem cell size in epidermal and intestinal stem cells. S. Xie1, G. Q. G. de Medeiros2, P. Liberali2, J. M. Skotheim1; 1Biology, Stanford University, Stanford, CA, 2Friedrich Miescher Institute for Biomedical Research, Basel, SWITZERLAND.
3:47 pm SG231 Cell size regulation in epithelial tissue. J. Devany, M. Gardel; University of Chicago, Chicago, IL.
4:02 pm SG232 Natural Gradient of Cell Size Regulates Genome Activation Pattern and Vertebrate Early Development. W. Qian, H. Chen, M. C. Good; Department of Cell and Developmental Biology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA.
Scientific Tracks: Physical Cell, Cellular Dynamics
Organizers: Matthew Kutys, University of California, San Francisco, Quinton Smith, University of California Irvine
Advancements in microfabrication technology, stem cell biology and organoid systems, synthetic biology, and cell mechanobiology, have permitted the design and assembly of complex 3D tissues in vitro. Research has increasingly realized the potential of engineered biomimetic models to recapitulate and control emergent cellular behaviors previously only accessible in vivo. By doing so, these systems have provided important mechanistic insight into factors governing the multiscale coordination of cell and tissue morphogenic processes, namely specific molecular signaling pathways, heterotypic cell interactions, and microenvironmental cues. This special interest subgroup will focus on how engineered 3D biomimetic systems are being designed and applied to provide biological insight where traditional in vivo and in vitro approaches are limiting, ultimately enhancing our understanding of the fundamental chemical and physical mechanisms that act across biological scales to direct cell and tissue morphodynamics.
1:45 pm Introduction.
1:47 pm SG233 Engineering epithelial shape and mechanics. X. Trepat; IBEC Barcelona, Barcelona, SPAIN.
2:07 pm SG234 Self-organized morphogenesis of a human neural tube in vitro by geometric constraints. E. Karzbrun1, A. H. Khankhel1, H. C. Megale1, S. M. K. Glasauer1, Y. Wyle1, G. Britton2, A. Warmflash2, K. S. Kosik1, E. D. Siggia3, B. I. Shraiman1, S. J. Streichan1; 1University of California Santa Barbara, Santa Barbara, CA, 2Rice University Houston, Houston, TX, 3The Rockefeller University, New York, NY.
2:19 pm SG235 From averages to ensembles- a statistical mechanical perspective on structural distributions of self-organizing tissues. V. Srivastava1, J. L. Hu2, J. C. Garbe1,3, M. R. Stampfer3, M. A. LaBarge4, Z. J. Gartner1,5; 1Pharmaceutical Chemistry, University of California San Francisco, San Francisco, CA, 2Department of Bioengineering, University of California San Francisco, San Francisco, CA, 3Life Science Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 4Beckman Research Institute, City of Hope, Duarte, CA, 5Chan Zuckerberg Biohub, San Francisco, CA.
2:31 pm SG236 The role of SGEF in tissue morphogenesis and organ development. M. Lovejoy, A. F. Rabino, T. Davuluri, G. Kreider-Letterman, R. Garcia-Mata; Biological Sciences, University of Toledo, Toledo, OH.
2:43 pm SG237 Disentangling the effect of confinement on keratocyte shape, size, and speed. E. C. Labuz1,2, M. J. Footer2, J. A. Theriot2; 1Program in Biophysics, Stanford University, Stanford, CA, 2Department of Biology and Howard Hughes Medical Institute, University of Washington, Seattle, WA.
2:55 pm Break.
3:07 pm SG238 Geometric control of intestinal organoid patterning. M. Lutolf; EPFL, Lausanne, SWITZERLAND.
3:27 pm SG239 In vivo study and synthetic engineering of mechanical rules for kidney tubule morphogenesis. J. M. Viola, C. M. Porter, A. Gupta, M. Alibekova Long, L. S. Prahl, A. J. Hughes; Department of Bioengineering, University of Pennsylvania, Philadelphia, PA.
3:39 pm SG240 Kinome-wide Screen Identifies Raf-MAPK Pathway as a Regulator of Hair Follicle Stem Cell Plasticity. L. C. Biggs1, C. Chacón-Martinez2, H. Toru3, B. Yadav1, S. A. Wickström1; 1Stem Cells and Metabolism Research Program, University of Helsinki, Helsinki, FINLAND, 2Max Planck Institute for Ageing, Cologne, GERMANY, 3Centre for Stem Cells and Regenerative Medicine, Kings College London, London, UNITED KINGDOM.
3:51 pm SG241 Notch1 Regulates Ductal Morphogenesis, Adherens Junctions, and Mitogenic Signaling Through a Transcription-Independent Mechanism. M. J. White, M. L. Kutys; Cell and Tissue Biology, University of California, San Francisco, San Francisco, CA.
4:03 pm SG242 Trans-epithelial fluid pumping performance of renal epithelial cells and hydraulic pressure gradient transduction. I. Choudhury, M. Benson, S. Sun; Institute for Nano-BioTechnology, Johns Hopkins University, Baltimore, MD.
Scientific Track: Cells in Distress & Disease
Organizers: Dana-Lynn Koomoa-Lange, University of Hawaii at Hilo, Michelle Martinez, Universidad Central del Caribe (School of Medicine)
Natural products, the structurally diverse family of small molecules produced by living organisms, provide the basis for most of our therapeutic agents. The environment on the islands of Hawaii and Puerto Rico provide a unique set of natural products with potent medicinal effects. However, these environments also present unique challenges that native people face while pursuing an education in cell biology and conducting cell biology research. A key aspect herein is to share information on how best to employ novel strategies to discover biological processes and effectively carry out drug discovery programs focusing on commonly used therapies in the Islands, while engaging PEER scientists in cell biology research. We will include talks on natural products research performed in Hawai’i and Puerto Rico, as well as additional research that focuses on relevant research experiences from PEER scientists.
1:45 pm Introduction.
1:48 pm SG243 Bioguided isolation of chemical constituents from Simarouba tulae, an endemic plant of Puerto Rico. C. A. Ospina; Inter American University of Puerto Rico Bayamon Campus, Bayamon, PR.
2:13 pm SG244 Effects of Ganoderma spp. extracts used as complementary therapy for aggressive breast cancers in Puerto Rico. G. Ortiz-Soto1, J. G. Cirino-Simonet1, A. C. Acevedo-Diaz2, I. J. Suarez-Arroyo1, T. J. Rios-Fuller3, S. Sagardia4, K. Miller4, M. M. Martinez-Montemayor1; 1Universidad Central del Caribe-School of Medicine, Bayamon, PUERTO RICO, 2University of Puerto Rico, Bayamon, PUERTO RICO, 3New York University, New York City, NY, 4Huerto Rico, LCC, Bayamon, PUERTO RICO.
2:33 pm SG245 Ganoderma lucidum Enhances Carboplatin Effect by Inhibiting the DNA Damage Response and Stemness in Inflammatory Breast Cancer. I. Suarez-Arroyo1, A. Acevedo-Díaz2, T. Ríos-Fuller3, G. Ortiz-Soto1, J. Reyes-Chea1, M. Martínez-Montemayor1; 1Universidad Central del Caribe-School of Medicine, Bayamon, PUERTO RICO, 2University of Puerto Rico, Bayamon, PUERTO RICO, 3New York University School of Medicine, NY, NY.
2:53 pm Break.
3:03 pm SG246 Ergosterol peroxide effects, sub-localization and potential biological targets in aggressive breast cancer models. M. M. Martínez-Montemayor1, T. Ling2, I. J. Suarez-Arroyo3, G. Ortiz-Soto4, C. L. Santiago-Negron1, M. Y. Lacourt-Ventura1, F. Rivas2; 1University of Central Del Caribe-School of Medicine, Bayamon, PUERTO RICO, 2Louisiana State University, Baton Rouge, LA, 3Universidad Central del Caribe-School of Medicine, Bayamon, PUERTO RICO, 4Universidad Central del Caribe - School of Medicine, Bayamon, PUERTO RICO.
3:28 pm SG247 Investigating the anti-cancer effects of La‘au Lapa‘au (Native Hawaiian plant-based medicine) and other traditional medicines. N. Sunada, D. Koomoa-Lange; University of Hawaii At Hilo, Hilo, HI.
3:48 pm SG248 Investigating Natural Products in Cancer Research: Engaging Native Hawaiian and Pacific Islander Students. D. Koomoa-Lange; University of Hawaii at Hilo, Hilo, HI.
4:13 pm Closing Remarks.
Scientific Tracks: Cellular Dynamics, Physical Cell
Organizers: Jonah Cool, Chan Zuckerberg Initiative, Catherine Galbraith, OHSU, Jim Galbraith, OHSU, Stefan Hinz, City of Hope National Medical Center, Theo Knijnenburg, Allen Institute for Cell Science, Manuel Leonetti, Chan Zuckerberg Biohub, Emma Lundberg, KTH Royal Institute of Technology, Ashok Prasad, Colorado State University, Denis Tsygankov, Georgia Tech, Jianhua Xing, University of Pittsburgh
Much of our knowledge of cells and how they function has been derived from observation. Technological developments are transforming imaging and image analysis, and the interior of the cell can now be visualized with greater fidelity. This creates new opportunities for cell biology. In particular, the synthesis of massive volumes of image data and integration with other omics modalities paves the way for a comprehensive characterization of cellular systems. But new challenges also arise: How do we make the most of image data? How to best combine different data types and the different scales they represent? What are the unique insights that can be gained through the integration of multiple modalities? And to what respect can imaging be a central bridge between other kinds of cellular measurements? In this subgroup we will explore how new advanced approaches to imaging and computational modeling can be used to characterize cells as complex systems and identify emergent properties across scales. Because this question lies at the nexus of biology, imaging technologies, computational models, and machine learning, this session will be highly interdisciplinary with speakers whose interests range from pattern recognition to cell biology.
1:45 pm Introduction.
1:47 pm SG249 Quantifying the dynamics of gene expression at multiple spatial and temporal scales in developing embryos. M. Mir; Cell and Developmental Biology, University of Pennsylvania, Children's Hospital of Philadelphia, Philadelphia, PA.
2:05 pm SG250 Seeing in vivo forces- development of light-producing intracellular nanosensors to quantify mechanical signaling in living cell chromosomes. M. V. Mukhina1, A. Butterfield2, R. Schaak2, N. Kleckner1; 1MCB, Harvard University, Cambridge, MA, 2The Pennsylvania State University, University Park, PA.
2:16 pm SG251 Using image-based reconstructions of cellular geometries for computational modeling. P. Rangamani; UCSD, La Jolla, CA.
2:34 pm SG252 Targeting intracellular organization via microscopy-based high-content phenotypic screening and generative neural networks. N. Kolet1, A. Shpigler2, S. Golan3, A. Zaritsky2; 1Ben-Gurion University of the Negev, Beer Sheva, ISRAEL, 2Ben Gurion University of the Negev, Beer Sheva, ISRAEL, 3Jerusalem College of Technology Lev Academic Center, Jerusalem, ISRAEL.
2:45 pm SG253 Using Biochemical Reconstitution to Understand the Emergent Behavior of Signaling Pathways. L. Case; Department of Biology, Massachusetts Inst Technology, Cambridge, MA.
3:03 pm Break.
3:15 pm Introduction.
3:17 pm SG254 Polaris: accurate spot detection for single-molecule FISH images with deep learning and weak supervision. E. C. Laubscher, N. Razin, E. Moen, W. Graf, D. Van Valen; California Inst Technol, Pasadena, CA.
3:28 pm SG255 Mapping cell structure across scales by fusing protein images and interactions. T. Ideker; Medical Genetics, University of California, San Diego, San Diego, CA.
3:46 pm SG256 Organelle topology is a new breast cancer cell classifier in 2D and 3D cultured systems: classification of 3D rendered organelle objects using high resolution imaging & machine learning. L. Wang1, J. Goldwag1, M. Bouyea1, J. Ward1, N. Maharjan2, A. Eladdadi2, M. Barroso1; 1Albany Medical College, Albany, NY, 2The College of Saint Rose, Albany, NY.
3:57 pm SG257 Characterizing the subnuclear micro-environments of genes by multi-modal data integration. A. Yildirim, L. Boninsegna, Y. Zhan, F. Alber; Department of Microbiology, Immunology & Molecular Genetics, University of California, Los Angeles, Los Angeles, CA.
Scientific Track: Cellular Dynamics
Organizers: Susan Dutcher, Washington University School of Medicine, Elif Nur Firat-Karalar, Koc University, Turkey, Moe Mahjoub, Washington University in St Louis
The centrosome-cilium complex is a major signaling hub in mammalian cells. These microtubule-based organelles play key roles during development and homeostasis by acting as a cellular antenna that provides a unique subcompartment to organize both intercellular and intracellular signaling events. Its composition is continually in flux and dynamically remodeled by internal and extracellular chemical and mechanical stimuli. Although progress has been made in uncovering the mechanisms by which cilia perform their signaling functions, much less is known about how internal signaling pathways are organized and staged, how cilia help to send messages to other cells, and how external cues are internalized. This proposed subgroup will address mechanisms that operate at various subcellular levels to ensure that proper ciliary-based signaling occurs at the right time and place.
The main questions to be addressed are:
- How are proteins targeted to the centrosome for entry into cilia?
- Staging of ciliary signaling proteins: what is the role of the ciliary pocket and ciliary base?
- Sending signals: Ectosome-mediated ciliary signaling
- Internalization of extracellular ciliary signals
1:45 pm Introduction.
1:50 pm SG258 Centriolar satellites act as transit sites for regulators cilium biogenesis and ciliary signaling. E. N. Firat-Karalar; Molecular Biology and Genetics, Koc University, Istanbul, TURKEY.
2:04 pm SG259 Using Cryo-ET to take an In Situ look at the Ciliary Base and Transition Zone, revealing native TZ structure and IFT train assembly in Chlamydomonas reinhardtii. H. G. van den Hoek1,2; 1Department of Molecular Structural Biology, Max Planck Institute of Biochemistry, Martinsried, GERMANY, 2Cell Architecture Lab, Helmholtz Zentrum München, Deutsches Forschungszentrum für Gesundheit und Umwelt, Munich, GERMANY.
2:18 pm SG260 Revealing membrane architecture directing the assembly of the primary cilium. Q. Lu1, H. Zhao1, A. Harned2, K. Narayan2, C. Westlake1; 1Laboratory of Cell and Developmental Signaling, Center for Cancer Research, NCI-Frederick, Frederick, MD, 2Center for Molecular Microscopy, Frederick National Laboratory, Frederick, MD.
2:32 pm SG261 Regulation of Primary Ciliogenesis by C-terminal Eps15 Homology Domain Proteins. T. Jones, N. Naslavsky, S. Caplan; University of Nebraska Medical Center, Omaha, NE.
2:46 pm SG262 Understanding Transducer Immobilization in Signaling Through the Primary Cilium. G. Garcia, III, B. Tsai, J. F. Reiter; Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA.
3:00 pm SG263 Interactions Between Tulp3 Tubby Domain Cargo Site and Arl13b Amphipathic Helix Promote Lipidated Protein Transport to Cilia. V. Palicharla1, S. Hwang1, E. Legué2, K. Liem2, S. Mukhopadhyay1; 1Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, TX, 2Department of Pediatrics, Yale University School of Medicine, New Haven, CT.
3:14 pm SG264 Deciphering the Ciliary Extracellular Vesicle (EV) Proteome. I. Nikonorova1, J. Wang1, A. Cope1, P. Tilton1, K. Power1, J. Walsh1, J. Akella1, A. Krauchunas2, P. Shah1, M. Barr1; 1Rutgers University, Piscataway, NJ, 2University of Delaware, Newark, DE.
3:28 pm SG265 The ciliiary necklace is involved in localization of mating signals in Chlamydomonas. S. K. Dutcher1, U. W. Goodenough2, R. Roth1; 1Genetics, Washington University Sch Med, St Louis, MO, 2Biology, Washington University in St Louis, St Louis, MO.
3:42 pm SG266 Ciliary Signaling Mechanisms directing mesenchymal stem cell fate. K. I. Hilgendorf; Biochemistry, University of Utah, Salt Lake City, UT.
3:56 pm SG267 Shedding light on the spatial and temporal organization and function of ciliary signaling in health and disease. J. Hansen1, F. Kaiser1, B. Stüven1, D. U. Mick2, D. J. P. Henderson3, D. Wachten1; 1Institute of Innate Immunity, Department of Biophysical Imaging, University of Bonn, Bonn, GERMANY, 2Saarland University, Homburg, GERMANY, 3Mironid Ltd., Glasgow, UNITED KINGDOM.
Scientific Tracks: Cellular Dynamics, Cells in Distress & Disease
Organizers: Taran Gujral, Fred Hutchinson Cancer Research Center, Henry Ho, University of California Davis
This special subgroup symposium focuses on the Wnt signaling pathway and its role in embryonic development and diseases. Wnt family proteins are growth factors that play critical roles in proliferation, migration, and invasion. Aberrant activation of Wnt signaling has been implicated in various human developmental disorders and malignancies of the colon, breast, liver, skin, brain, and prostate. We will hear from leading experts who study the role of Wnt in normal development and disease states.
1:45 pm Introduction.
1:49 pm SG203 Targeting FZD7 signaling in development and disease. K. Willert; UC San Diego, La Jolla, CA.
2:04 pm SG204 The ubiquitin ligase HUWE1 regulates WNT signaling through a new mechanism. A. Lebensohn, J. McKenna, Y. Wu, P. Sonkusre; Laboratory of Cellular and Molecular Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD.
2:19 pm SG205 The dynamics of the APC-Axin complex determines Wnt/β-catenin pathway’s activity. W. Ma1, M. Chen2, H. Kang3, B. Luan1, X. He4, M. W. Kirschner1; 1Harvard Medical School, Boston, MA, 2Children's Hospital, Boston, MA, 3Harvard Medical School, East Walpole, MA, MA, 4Boston Children's Hospital, Boston, MA.
2:34 pm SG206 Bcl-2 up-regulation mediates taxane resistance downstream of APC loss. S. Maloney, J. Prosperi; Indiana University Sch Med-South Bend, South Bend, IN.
2:49 pm Break.
2:59 pm SG207 Cross-talk between Non-canonical Wnt and Transforming growth factor beta pathways control the epithelial-mesenchymal transition and cell migration. M. Chan, T. S. Gujral; Fred Hutchinson Cancer Research Center, Seattle, WA.
3:14 pm SG208 Wnt5a-ror regulates cell migration and contractility via rhoa-myosin-actin (rma) axis. S. Srinivasan, H. Ho; Cell Biology and Human Anatomy, University of California, Davis, Davis, CA.
3:29 pm SG209 Wnt11 family dependent morphogenesis during frog gastrulation is marked by the cleavage furrow protein anillin. E. Van Itallie, C. Field, T. Mitchison, M. Kirschner; Systems Biology, Harvard Medical School, Boston, MA.
3:44 pm SG210 Wnt/planar cell polarity signaling contributes to glioblastoma multiforme invasiveness. C. A. Dreyer1, K. VanderVorst1, G. Bell2, P. Sood1, M. Hernandez1, J. Angelastro3, S. Collins4, K. L. Carraway, III1; 1Biochemistry and Molecular Medicine, UC Davis School of Medicine, Sacramento, CA, 2Microbiology and Molecular Genetics, University of California, Davis, Davis, CA, 3Molecular Biosciences, UC Davis School of Veterinary Medicine, Davis, CA, 4Microbiology and Molecular Genetics, University of California, Davis, Sacramento, CA.
For Subgroup Organizers:
- Identify and invite potential speakers to participate in your Subgroup session. Names of speakers are submitted during the application process.
- Inform all invited speakers to submit abstracts by August 3, 2021. All Subgroup speakers must submit abstracts through the Cell Bio Virtual 2021 Abstract Submission system.
- Review abstracts submitted to your Subgroup topic and select abstracts to design and schedule your session. The review period will take place from August 5 through August 16.
- Register for Cell Bio Virtual 2021. All speakers and organizers must be registered for Cell Bio Virtual 2021. Admission will not be granted if individuals are not registered.
- Communicate with speakers about their role and the meeting policies. Ensure speakers are registered and understand they are responsible for the registration fee. ASCB will provide organizers with an email template for inviting speakers upon selection notification.
Diversity and Inclusion
ASCB is committed to ensuring that a diversified and inclusive program is presented to our attendees. Subgroup Organizers will be required to ensure talks selected are from presenters with diverse ethnic, gender, and research backgrounds, career levels, and geographic locations. Accepted Subgroup organizers will be required to participate in a Diversity, Equity, and Inclusivity (DEI) training provided by ASCB.
Availability Requirements
Subgroup Organizers must be available during the following time periods:
- Thursday, August 5 through Monday, August 16 - Abstract Review and Selection Period
- Monday, December 6 through Friday, December 10- Available virtually on the day of Subgroup session at a designated date and time
Miscellaneous
You and your accepted speakers must agree to the following terms and conditions:
- Not to defame or slander/libel anyone
- Not to use copyright/trademark materials without permission
- Not to speak on anything other than what was agreed to
Timeline & Key Dates
| Subgroup Organizer Applications | Applications for Subgroup Organizers will be accepted for consideration in organizing a Subgroup session. | Deadline: May 3 |
| Subgroup Organizer Review | Program Committee Chairs will review applications to select Subgroup Organizers. | Late April to Late May |
| Subgroup Organizer Notifications | Applicants will receive selection notifications. | May 26/27 |
| Abstract Submission | Abstracts will be accepted for consideration for a talk in one of the Subgroup sessions. | Opens early June; Closes August 3 |
| Abstract Review | Subgroup Organizers will review and assign the abstracts to their Subgroup sessions. | August 5 to August 16 |
| Session Scheduling | Subgroup Organizers will finalize the schedule for their Subgroup sessions. | August 5 to August 16 |
| Speaker Notification | Abstract submitters will receive selection notifications. | Week of September 15 |