Highlighted By: Adriana Mantegazza, Thomas Jefferson University
Preprint DOI: https://doi.org/https://doi.org/10.1101/2023.08.11.553042
This preprint has been assigned the following Badges:
- Conventional dendritic cells type 1 (cDC1s) specialize in antigen “cross-presentation”, a mechanism that involves the presentation of exogenous antigens on major histocompatibility complex class I (MHC-I) proteins. While this process is essential in triggering anti-viral and anti-tumor immunity, the molecular mechanisms that facilitate cargo export to the cytosol to promote MHC-I presentation after phagocytosis are largely unknown.
- Using correlative confocal and focused ion beam scanning electron microscopy, along with FRET and FRAP approaches, the authors show that phagosomal recruitment of the pore-forming protein apolipoprotein 7C (APOL7C) leads to phagosomal membrane rupture, leading to phagosomal content exit. Notably, APOL7C recruitment is dependent on NADPH oxidase-induced phagosomal membrane depolarization.
- These findings suggest APOL7C as a novel phagosomal effector required for efficient phagosomal cargo exit and subsequent antigen cross-presentation in cDC1s.
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