PAIR-UP Advanced Imaging Workshop for Black Imaging Scientists
April 11th – 14th, 2023 | Time: 9AM – 5PM EDT | Location: UNC at Chapel Hill
The PAIR-UP Advanced Imaging Workshop is designed to provide training in the following major areas:
1. Advanced microscopy acquisition considerations for imaging live cells using transmitted light, fluorescent probes, and fluorescent biosensors, using systems designed for automated imaging workflows (Nikon Ti2), fast and gentle imaging (resonance confocal scanning and spinning disk confocal microscopy), spectral imaging (Zeiss spectral detector and Leica FALCON lifetime imaging with a pulsed white-light laser) and high-resolution imaging of live samples (TILT lightsheet and 3X tau-STED).
2. Appropriate sample preparation, including choice of fluorescent probes and fluorescent proteins, and refractive index matching, particularly for live imaging experiments.
3. Ethical, reproducible and rigorous considerations for image analysis and presentation of live imaging experiments, including both proprietary and open-source software options.
Black imaging scientists are strongly encouraged to attend
Who Should Attend?
• Principal Investigator (PAIR-UP Black Imaging scientist or
personnel from the PI’s lab)
• Postdoctoral Fellow (in lab of PAIR-UP PI)
Microscopes Used:
Fast and gentle imaging: Olympus FV3000RS confocal microscope OR Andor Dragonfly spinning disk confocal microscope (better for bigger samples, fewer colors)
Spectral imaging: Zeiss 880 confocal with spectral detector OR Leica SP8X with FALCON
Standard development of automated widefield imaging workflows: Nikon Ti2 widefield microscope
High-resolution live imaging: Mizar TILT light-sheet OR Leica 3x tau-STED
Learning objectives:
- Understand and explain the optical principles of each instrument
- Acquire digital images for documentation and analysis that accurately represent the biological sample
- Interpret the data and understand and assess potential artifacts
- Perform quantitative measurements from images using software on the instrument and open-source software
- Determine which instrument is most suitable to acquire data to answer a specific biological question
- Establish a stepwise workflow protocol to optimize data collection, particularly for live samples, on the instrument
Featured Instructors:
Michelle Itano, PhD,: Assistant Professor, Department of Cell Biology and Physiology, Director of the UNC Neuroscience Microscopy Core Facility, University of North Carolina at Chapel Hill
Tessa-Jonne Ropp, PhD: Research Associate, UNC Neuroscience Microscopy Core Facility, University of North Carolina at Chapel Hill
Pablo Ariel, PhD: Associate Professor, Department of Pathology and Laboratory Medicine, Director of the Microscopy Services Laboratory, University of North Carolina at Chapel Hill
Wendy Salmon, MA: Director of the Hooker Imaging Core, Department of Cell Biology and Physiology, University of North Carolina at Chapel Hill
Paul Maddox, PhD: Associate Professor and William Burwell Harrison Scholar, Department of Biology, University of North Carolina at Chapel Hill
Samples provided by:
Amy Gladfelter, PhD: Associate Professor, Department of Biology, University of North Carolina at Chapel Hill
Sarah Cohen, PhD: Assistant Professor, Department of Cell Biology and Physiology, University of North Carolina at Chapel Hill
Klaus Hahn, PhD: Thurman Professor of Pharmacology, Department of Pharmacology, University of North Carolina at Chapel Hill
Cost To Attend: The workshop is free to accepted applicants.
Application Deadline: February 24th 2023