Scientific Workshops
Scientific Workshops by Day
Sunday, December 8, 4:15 pm-6:50 pm
Room 151B
How the interactions between individual molecules give rise to order on the cellular scale is a central question in cell biology. To understand this problem, it is important to combine experiments in living cells with in vitro reconstitution experiments, to perform rigorous quantitative analysis and develop new methodologies. Our workshop will cover recent advances in experimental techniques to quantitatively characterize the behavior of dynamic systems, such as super-resolution microscopy, single-molecule imaging and its combination with mechanical manipulation, the in vitro reconstitution of complex protein systems, as well as novel computational approaches. We will discuss how to integrate data obtained from different spatial and temporal scales to obtain a more complete understanding of the mechanisms giving rise to a living cell.
Organizers:
Chip Asbury, University of Washington
Ibrahim Cisse, Massachusetts Institute of Technology
Martin Loose, IST Austria
Speakers:
4:15 pm Brief introduction
4:20 pmCooperative Ordering of Treadmilling: How to Increase Precision of the Bacterial Cell Division Machinery. Martin Loose, IST Austria
4:35 pm Discussion, changeover
4:45 pm Speed Oscillations Lend Insight into Cytokinesis Contractility. Amy Shaub Maddox, University of North Carolina at Chapel Hill
5:00 pm Discussion, changeover
5:10 pm Building Kinetochores de novo: Biophysical Investigation of Kinetochore Assembly and Function. Josh Larson, University of Washington
5:25 pm Discussion, changeover
5:35 pm Learning About Mechanisms When Modeling Projects Go Wrong. Meredith Betterton, University of Colorado Boulder
5:50 pm Discussion, changeover
6:00 pm Mechanisms Controlling the Strength and Duration of Phosphatidylinositol Phosphate Lipid Signaling. Scott Hansen, University of Oregon
6:15 pm Discussion, changeover
6:25 pm Why Don't Plants Get Sunburn? Gabriela Schlau-Cohen, Massachusetts Institute of Technology
6:40 pm Discussion, concluding remarks
Monday, December 9, 4:15 pm-6:50 pm
Room 151B
Optical microscopy and optical manipulation of biological systems are powerful tools to both observe and interrogate cellular behavior. This workshop gives an update on innovative developments in these fields, spanning from super-resolution microscopy and functional imaging, to optogenetics. We will focus on the technical aspects of implementing these methods and applications to cell biology, and a town-hall discussion providing ample time for questions will follow talks. All are welcome to participate.
Organizers:
Michael Lampson, University of Pennsylvania
Ricardo Henriques, Medical Research Council Laboratory for Molecular Biology
Speakers:
Gentler and Faster Optical Nanoscopy for Brain Cell Imaging. Ilaria Testa, KTH Royal Institute of Technology
In vivo and in vitro Applications of Quantitative Fluorescence Lifetime FRET Imaging. Margarida Barroso, Albany Medical Center
Democratizing Live-Cell Super-Resolution Microscopy Enabled by Open-Source Analytics in Imagej. Ricardo Henriques, University College London & The Francis Crick Institute
Optogenetic Control of Cell Biology: Insights into Mitotic Chromosome Segregation. Michael Lampson, University of Pennsylvania.
Title TBD. Alexander Deiters, University of Pittsburgh
Tuesday, December 10, 4:15 pm-6:50 pm
Room 151B
Supported by Illumnia
Single-cell transcriptomics techniques offer the exciting ability to discover and catalog cell types and states from essentially any tissue or organism. As such, these methods are driving large-scale efforts to systematically map the cell types across all tissues in entire model organisms, and these reference atlases promise to provide fundamental new insights into tissue biology and to be transformative resources that will drive the next wave of discoveries into disease mechanism. Nonetheless, to meet the ambitious goals of such cell-atlas efforts, improvements are needed on all aspects of single-cell methodology. In this workshop, several investigators in the growing field of single-cell analysis will introduce state-of-the-art developments in single-cell experimental methodology, computational analysis, as well as the burgeoning field of spatial genomics.
Organizers:
Jeff Moffitt, Harvard University
Fei Chen, Massachusetts Institute of Technology
Speakers:
Jeffrey Moffitt, Harvard University
Fei Chen, Massachusetts Institute of Technology
Karthik Shekhar, University of California, Berkeley
Peter Sims, Columbia University
4:15 pm: Workshop introduction
4:20 pm: Slide-Seq: A Scalable Technology for Measuring Genome-Wide Expression at High Spatial Resolution, Dr. Fei Chen, Broad Institute
4:50 pm: Imaging the transcriptome: Constructing cellular atlases with MERFISH, Dr. Jeffrey Moffitt, Boston Children's Hospital and Harvard Medical School
5:20 pm: scRNA-seq and SCOPE-seq Uncover a Secreted Regulator of Adult Neurogenesis in the Mouse Brain, Dr. Peter Sims, Columbia University
5:50 pm: Of mice and monkeys: single-cell analysis of the vertebrate retina, Dr. Karthik Shekhar, University of California, Berkeley
6:20 pm: Panel discussion on emerging directions in single-cell analysis