PAIR-UP Imaging Workshop @ Vanderbilt

The PAIR-UP Advanced Imaging Workshop is designed to provide training in the following major areas:

1. Advanced fluorescence microscopy using fluorescent probes, fluorescent biosensors, and including TIRF, Confocal laser scanning microscopy, and light-sheet microscopy.

2. Super-resolution techniques for live cell imaging including Zeiss Airyscan and structured illumination microscopy (SIM). In addition, the workshop will provide guidance on appropriate sample preparation, including choice of fluorescent probes and fluorescent proteins, and refractive index matching. The workshop is a hands-on workshop. Black imaging scientists are strongly encouraged to attend if you use or plan to use advanced imaging equipment

Who Should Attend?
• Principal Investigator (PAIR-UP Black Imaging scientist or
personnel from the PI’s lab)
• Postdoctoral Fellow (in lab of PAIR-UP PI)
• Research Assistant/technician (in lab of PAIR-UP PI)

Microscopes Used:
• Zeiss Z1 Light Sheet microscope
• Single Objective Oblique Light Sheet
• Nikon SIM
• Biophotonics pre-commercial Lattice Light Sheet

Learning objectives: After completing the workshop, participants will be able to:

1. Understand and explain the optical principles of the instrument 
2. Acquire digital images for documentation and analysis that accurately represent the biological sample
3. Interpret the data and understand and assess potential artifacts
4. Perform quantitative measurements from images using software on the instrument
5. Determine which instrument is most suitable to acquire data to answer a specific biological question
6. Establish stepwise workflow protocol to optimize data collection on the instrument

Featured Instructors:

Bryan Millis: Research Assistant Professor, Department of Cell and Developmental Biology, Vanderbilt University

Jenny Schafer: Research Associate Professor, Department of Cell and Developmental Biology, Managing Director, Cell Imaging Shared Resource (CISR), Vanderbilt University

Anita Mahadevan-Jansen: Professor of Biomedical Engineering, School of Engineering, Orrin H. Ingram Professor of Engineering Director of Undergraduate Studies in Biomedical Engineering Professor of Neurological Surgery, Director of the Biophotonics Center at Vanderbilt University

Selection Process: The PAIR-UP team will review all workshop applications after the deadline and make selections.  We have space for 20 participants.  Selected applicants will be notified of acceptance and next steps for attending. 

Cost To Attend: All expenses will be reimbursed for those selected for the workshop.

Application Deadline: April 15, 2022