Minisymposia Sessions by Day
Sunday, December 8, 4:15 pm to 6:50 pm
Co-Chairs: Jennifer Hood-DeGrenier, Worcester State University; and Leocadia Paliulis, Bucknell University
4:15 pm Introduction
4:20 pm M1 ASCB Declaration on Effective and Inclusive Undergraduate Biology Education: from National Vision to Widespread Action. V. A. Segarra1, M. L. Styers2, E. L. Dolan3; 1High Point University, High Point, NC, 2Birmingham-Southern College, Birmingham, AL, 3University of Georgia, Athens, GA.
4:30 pm M2 Using Modeling and Animation to Overcome Previously-Held Misconceptions. L. V. Paliulis; Bucknell University, Lewisburg, PA.
4:45 pm M3 Developing a New Measure of Reasoning in Introductory Biology Education. F. E. Nelson1, T. Fechter2, T. Dai3, J. G. Cromley4, Y. Du4; 1Temple University, Philadelphia, PA, 2Sole Proprietor, Monterey, CA, 3University of Illinois at Chicago, Chicago, IL, 4University of Illinois at Urbana-Champaign, Champaign, IL.
5:05 pm M4 Expert-novice Comparison Reveals Pedagogical Implications for Students’ Analysis of Primary Literature. M. Segura-Totten, A. Nelms; University of North Georgia, Dahlonega, GA.
5:25 pm M5 Building a Successful High School Outreach Project with Undergraduate Mentors: The Prince Edward County Environmental Molecular Biology Institute (PECEMBI). M. J. Wolyniak1, A. R. Barber2; 1Hampden-Sydney College, Hampden-Sydney, VA, 2Longwood University, Farmville, VA.
5:45 pm M6 Six New York City (NYC) Medical Schools Will Offer Two Summer-long, Eight Weeks/summer, Professional Development (PD) Programs for New York City’s Secondary Science Teachers Beginning in Summer 2021. S. C. Silverstein1, J. Wiederhorn2; 1Columbia University Coll Phys & Surg, New York City, NY, 2Associated Medical Schools of New York, New York City, NY.
6:05 pm M7 An Integrated Undergraduate Steam Course: The Art and Science of Cell Death. S. Walsh1, A. Stewart2, J. Almond2, S. Berenguer2; 1Soka University of America, Aliso Viejo, CA, 2Rollins College, Winter Park, FL.
6:25 pm M8 A Data Analysis and Literature Intensive Undergraduate Course That Positively Impacts Student Ability and Confidence in Scientific Critical Thinking Skills and Increases Post-graduation Success. K. K. Resendes; Westminster College, New Wilmington, PA.
6:45 pm M9 Promoting Biology Literacy through a Non-majors First-year Seminar Focused on Cancer. J. Hood-DeGrenier; Worcester State University, Worcester, MA.
Co-Chairs: Bungo Akiyoshi, University of Oxford; and Sylvia Erhardt, Center for Molecular Biology Heidelberg University
4:15 pm Introduction
4:20 pm M10 Matchmaking during Meiosis: How Chromosomes Recognize Their Homologous Partners. G. V. Caldas1, F. Wu1, A. F. Dernburg1,2,3,4; 1Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, 2Howard Hughes Medical Institute, Chevy Chase, MD, 3California Institute for Quantitative Biosciences, Berkeley, CA, 4Lawrence Berkeley National Laboratory, Berkeley, CA.
4:35 pm M11 A Time-Resolved Network of Meiotic Chromosome Associated Proteins. S. Ur, R. Suhandynata, H. Zhou, K. Corbett; UCSD, La Jolla, CA.
4:50 pm M12 Retrotransposons in Drosophila Male Germline Stem Cells Maintain Ribosomal DNA Copy Number. J. O. Nelson1,2, Y. M. Yamashita1,2; 1University of Michigan, Ann Arbor, MI, 2HHMI, Ann Arbor, MI.
5:05 pm M13 Transposable Element-driven Reorganisation of 3D Chromatin during Early Embryonic Development. J. M. Vaquerizas; MPI for Molecular Biomedicine, Muenster, GERMANY.
5:20 pm M14 Localization of Drosophila Cenp-A to Non-centromeric Sites Depends on the Nurd Complex. E. Demirdizen1, S. Erhardt1, M. Spiller-Becker1,2, A. Förtsch1, A. Bergner1, B. Hessling3; 1ZMBH - Center for Molecular Biology Heidelberg, Heidelberg, GERMANY, 2Active Motif, Carlsbad, CA, 3DKFZ, Heidelberg, GERMANY.
5:35 pm M15 Centromere Incompatibility as the Basis for Chromosome Segregation Defects in Inviable Xenopus Hybrids. M. Kitaoka, R. Heald; University of California, Berkeley, Berkeley, CA.
5:50 pm M16 Understanding Unconventional Kinetoplastid Kinetochores. B. Akiyoshi, G. Marcianò, H. Hayashi, M. Ishii, P. Ludzia, O. Nerusheva; University of Oxford, Oxford, UNITED KINGDOM.
6:05 pm M17 A New Hi-C Method Reveals the Conformation of Sister Chromatids. M. Mitter1, C. Gasser2, Z. Takacs1, R. Stocsits3, W. Tang3, S. L. Ameres1, J. Peters3, A. Goloborodko4, R. Micura2, D. W. Gerlich1; 1Institute of Molecular Biotechnology of the Austrian Academy of Sciences, Vienna BioCenter, Vienna, AUSTRIA, 2Institute of Organic Chemistry and Center for Molecular Biosciences (CMBI), Leopold-Franzens University, Innsbruck, AUSTRIA, 3Research Institute of Molecular Pathology, Vienna BioCenter, Vienna, AUSTRIA, 4Department of Physics, Massachusetts Institute of Technology, Cambridge, MA.
6:20 pm M18 Reconstitution of Cohesin and Condensin-mediated DNA Loop Extrusion in Xenopus Egg Extracts. S. Golfier, T. Quail, J. Brugues; Max Planck Institute, Dresden, GERMANY.
6:35 pm M19 Centromere Strength Is Transgenerationally Inherited through the Male But Not Female Germline. A. Das, V. Fu, B. E. Black, M. A. Lampson; University of Pennsylvania, Philadelphia, PA.
Co-Chairs: Lydia Finley, Memorial Sloan-Kettering Cancer Center; and Matthew Vander Heiden, Massachusetts Institute of Technology
4:15 pm Introduction
4:20 pm M20 Metabolic Coordination of Cancer Cell Fate. L. Finley; Memorial Sloan Kettering Cancer Ctr, New York, NY.
4:35 pm M21 The Nutrient Microenvironment of Tissues and Tumors Affects the Metabolism of Resident Cells. A. Muir; University of Chicago, Chicago, IL.
4:50 pm M22 The Creatine Phosphagen System in Mechanoresponsive in Pancreatic Ductal Adenocarcinoma Cells and Fuels Invasive Behaviour. V. Papalazarou1, T. Zhang2, N. Paul1, M. Cantini3, O. Maddocks2, M. Salmeron-Sanchez3, L. Machesky1; 1CRUK Beatson Institute, Glasgow, UNITED KINGDOM, 2Institute of Cancer Sciences, University of Glasgow, Glasgow, UNITED KINGDOM, 3Centre for the Cellular Microenvironment, University of Glasgow, Glasgow, UNITED KINGDOM.
5:05 pm M23 Accumulation of Systemic Succinate Controls Activation of Adipose Tissue Thermogenesis. E. Mills; Dana-Farber Cancer Institute, Boston, MA.
5:20 pm M24 Characterization of Mitochondrial Metabolic Oscillations in Live Rodents. Y. Ng1, D. Chen1, W. Losert2, R. Weigert1; 1Laboratory of Cellular and Molecular Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, 2College of Computer, Mathematical, and Natural Sciences, University of Maryland, College Park, MD.
5:35 pm M25 Branched-Chain Amino Acids Control Mitochondrial Metabolite Carriers via the Mitochondrial-Derived Compartment Pathway. M. Schuler, A. M. English, J. M. Shaw, A. L. Hughes; University of Utah, Department of Biochemistry, Salt Lake City, UT.
5:50 pm M26 Intersection of the Golgi Stress Response and Redox Homeostasis in Huntington's Disease. B. D. Paul, S. H. Snyder; Johns Hopkins University School of Medicine, Baltimore, MD.
6:05 pm M27 Aquaporin-7 Is a Metabolic Sensor That Regulates Response to Cellular Stress in Breast Cancer. C. Dai, V. Charlestin, M. Wang, N. Dovichi, J. Li, L. Littlepage; University of Notre Dame, Notre Dame, IN.
6:20 pm M28 Metabolic Coordination of Stem Cell Fate Drives Tumor Initiation. S. Baksh1, P. Todorova2, S. Gur-Cohen1, E. Fuchs1, L. Finley2; 1The Rockefeller University, New York, NY, 2Memorial Sloan Kettering Cancer Center, New York, NY.
6:35 pm M29 Metabolic Limitations of Cell Proliferation. M. Vander Heiden; Koch Institute for Integrative Cancer Research at MIT, Cambridge, MA.
Co-Chairs: Prachee Avasthi, University of Kansas Medical Center; and Jessica Feldman, Stanford University
4:15 pm Introduction
4:20 pm M30 Centriole Motility and Subcellular Positioning Require Pericentrin-like-protein and Kinesin-1. M. R. Hannaford, Z. T. Swider, B. J. Galletta, C. J. Fagerstrom, N. M. Rusan; National Heart, Lung and Blood Institute, Bethesda, MD.
4:35 pm M31 In Vivo Proximity Labeling of PTRN-1/Patronin with TurboID Reveals Novel Components of Non-centrosomal MTOCs in Epithelial Cells. A. D. Sanchez1, T. Branon2, A. Y. Ting1, J. L. Feldman1; 1Stanford University, Stanford, CA, 2MIT, Cambridge, MA.
4:50 pm M32 Volumetric Electron Microscopy Reveals New Details of Primary Cilia Organization and Potential Function in the Brain. C. Ott1, R. Torres2, A. Weigel1, N. da Costa2, J. Lippincott-Schwartz1; 1HHMI Janelia Research Campus, Ashburn, VA, 2Allen Institute, Seattle, WA.
5:05 pm M33 Ift Trains Fragment Into ‘Carts’ of IFT-A, -B, and Dynein at the Ciliary Tip. J. L. Wingfield1, I. Mengoni1, P. Liu1, J. Dai1, Y. Hou2, W. Zhao2, G. B. Witman2, K. Lechtreck1; 1Department of Cellular Biology, University of Georgia, Athens, GA, 2Department of Cell and Developmental Biology, University of Massachusetts Medical School, Worcester, MA.
5:20 pm M34 Dynamic Basal Body-associated Striated Fibers Promote Ciliary Array Organization through Basal Body Coupling and Cortical Interactions. A. Soh1, T. van Dam2, A. Stemm-Wolf1, A. Pham1, G. Morgan3, E. O’Toole3, C. Pearson1; 1University of Colorado Anschutz Medical Campus, Aurora, CO, 2Utrecht University, Utrecht, NETHERLANDS, 3University of Colorado at Boulder, Boulder, CO.
5:35 pm M35 Arp2/3-mediated Branched Actin Networks Play a Role in Ciliary Assembly and Maintenance in Chlamydomonas. B. M. Bigge, P. Avasthi; University of Kansas Medical Center, Kansas City, KS.
5:50 pm M36 Generation of Stress Fibers from the Cortical Actomyosin Meshwork. J. Lehtimäki, K. Rajakylä, S. Tojkander, P. Lappalainen; University of Helsinki, Helsinki, FINLAND.
*6:05 pm M37 Reach Out and Touch Fate: Cytonemes in Sonic Hedgehog Signal Propagation. E. T. Hall, D. P. Stewart, S. K. Ogden; St. Jude Children's Research Hospital, Memphis, TN.
6:20 pm M38 Measured Contractile Ring Component Dynamics Inform Agent-based Models of Animal Cell Cytokinesis. D. Cortes1, M. DiSalvo1, N. Allbritton1, F. Nedelec2, P. Maddox1, A. S. Maddox1; 1UNC Chapel Hill, Chapel Hill, NC, 2University of Cambridge, Cambridge, UNITED KINGDOM.
6:35 pm M39 Neurite Morphogenesis Requires Suppression of the Lamellar Protrusions of the Cell Body by Septin 7. M. R. Radler, E. Spiliotis; Drexel University, Philadelphia, PA
*MBoC Paper of the Year Awardee
Co-Chairs: Marta Miasczynska, European Molecular Biology Laboratory; and Mary Munson, University of Massachusetts Medical School
4:15 pm Introduction
4:20 pm M40 Protein Droplets Catalyze Assembly of Endocytic Vesicles. K. J. Day1, G. K. Kago1, J. B. Richter2, C. C. Hayden1, E. M. Lafer2, J. C. Stachowiak1; 1Biomedical Engineering, University of Texas at Austin, Austin, TX, 2Biochemistry, University of Texas Health Science Center at San Antonio, San Antonio, TX.
4:35 pm M41 A Kinetic Analysis of Secretory and Vacuolar Protein Sorting Subdivides the TGN Stage of Golgi Maturation. J. C. Casler, B. S. Glick, PhD; University of Chicago, Chicago, IL.
4:50 pm M42 COPI and COPII Cooperate at ER Exit Sites to Support ER-to-Golgi Protein Trafficking Revealed by 3D Ultrastructure Analyses and Live-Cell Imaging. A. Weigel1, C. Chang1, G. Shtengel1, D. Hoffman1, M. Freeman1, C. S. Xu1, S. Pang1, E. Betzig2, H. Hess1, J. Lippincott-Schwartz1; 1Janelia, Ashburn, VA, 2University of California, Berkeley, CA.
5:05 pm M43 Tango1 Assembles a Machine to Select and Export Collagens from the Endoplasmic Reticulum. I. Raote1, F. Campelo2, V. Malhotra1; 1Centre for Genomic Regulation, Barcelona, SPAIN, 2The Institute of Photonic Sciences, Barcelona, SPAIN.
5:20 pm M44 Synthetic Lethality between Vps4a and Vps4b Triggers an Inflammatory Response in Colorectal Cancer. M. Miaczynska1, E. Szymanska1, P. Nowak1, K. Kolmus1, M. Cybulska2, K. Goryca2, E. Derezińska-Wołek3, A. Szumera-Ciećkiewicz3, M. Brewińska-Olchowik4, A. Grochowska5, K. Piwocka4, M. Prochorec-Sobieszek3, M. Mikuła2; 1International Institute of Molecular and Cell Biology, Warsaw, POLAND, 2Department of Genetics, Maria Skłodowska-Curie Institute-Oncology Centre, Warsaw, POLAND, 3Department of Pathology and Laboratory Medicine, Maria Skłodowska-Curie Institute-Oncology Centre, Warsaw, POLAND, 4Laboratory of Cytometry, Nencki Institute of Experimental Biology, Warsaw, POLAND, 5Department of Genetics, Maria Skłodowska-Curie Institute-Oncology Cent, Warsaw, POLAND.
5:35 pm M45 Tumor Protein D54 Defines a New Class of Intracellular Transport Vesicle. G. Larocque, S. J. Royle, P. J. La-Borde, N. I. Clarke, N. J. Carter; University of Warwick, Coventry, UNITED KINGDOM.
5:50 pm M46 Analysis of Retromer Complex Dynamics on Supported Lipid Bilayers. C. L. Deatherage1, J. Nikolaus2, E. Karatekin2, C. G. Burd1; 1Yale University, New Haven, CT, 2Yale University, West Haven, CT.
6:05 pm M47 CMEpi, a Potent and Selective Structure-based Inhibitor of Clathrin-mediated Endocytosis. Z. Chen, R. Mino, M. Mettlen, P. Michaely, M. Bhave, D. K. Reed, S. L. Schmid; UT Southwestern Medical Center, Dallas, TX.
6:20 pm M48 Exocyst Conformational Changes Control Interactions with SNARE and Sec1/Munc18 Proteins. D. Lepore1, M. Feyder1, L. Martinez-Nunez1, L. Kenner2, A. Czuchra1, G. Rossi3, P. Brennwald3, A. Frost2, M. Munson1; 1Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA, 2Department of Biochemistry and Biophysics, University of California, San Francisco, CA, 3Department of Cell Biology and Physiology, University of North Carolina School of Medicine, Chapel Hill, NC.
6:35 pm M49 Revealing the Mechanism That Controls Fusion Pore Dynamics in Giant Secretory Vesicles. T. Biton, K. Kumari, N. Scher, E. D. Schejter, B. Shilo, O. Avinoam; Weizmann Inst Science, Rehovot, ISRAEL.
Co-Chairs: Anne Carpenter, Broad Institute; and Lillian Fritz-Laylin, University of Massachusetts, Amherst
4:15 pm Introduction
4:20 pm M50 Mechanism of Mismatch Tolerance Difference between Rad51 and Dmc1 in Homologous Recombination. J. Xu, L. Zhao, R. Liang, C. Chen, H. Wang; Tsinghua University, Beijing, CHINA.
4:35 pm M51 Effects of Phase Separation on Dynamics of Polycomb Proteins Revealed by Live-cell Single-molecule Imaging. X. Ren; University of Colorado Denver, Denver, CO.
4:50 pm M52 Morphologically Discrete ER Subdomains Support Synthesis of Different Types of Protein. H. Choi1, Y. Liao1, Y. J. Yoon2, J. Grimm1, L. D. Lavis1, R. H. Singer1,2, J. Lippincott-Schwartz1; 1Janelia Research Campus, Ashburn, VA, 2Albert Einstein College of Medicine, Bronx, NY.
5:05 pm M53 Super-Resolution Microscopy Elucidates Curvature Generation by Endocytic Clathrin Coats in Live Cells and Tissues. N. Willy1, J. Ferguson1, S. Silahli1, C. Cakez2, F. Hasan1, H. Chang3, A. Travesset4, R. Zandi5, S. Li5, D. Li6, E. Betzig7, E. Cocucci1, C. Kural1; 1The Ohio State University, Columbus, OH, 2University of New Mexico, Albuquerque, NM, 3Purdue University, West Lafayette, IN, 4Iowa State University, Ames, IA, 5University of California, Riverside, Riverside, CA, 6Chinese Academy of Sciences, Beijing, CHINA, 7University of California, Berkeley, Berkeley, CA.
5:20 pm M54 Self-organization and Load Adaptation by the Mammalian Endocytic Actin Network: Integrating Modeling with Experiment. M. Akamatsu1, R. Vasan2, D. Serwas1, M. Ferrin1, P. Rangamani2, D. G. Drubin1; 1UC Berkeley, Berkeley, CA, 2UC San Diego, La Jolla, CA.
5:35 pm M55 Intrinsic Constraint of the Phenotypic Plasticity of the Actin Cytoskeleton Reveals Limited Attractor States. P. W. Gunning1, N. S. Bryce1, T. W. Failes1, J. R. Stehn1, K. Baker2, S. Zahler3, I. Dedova1, G. M. Arndt1, B. T. Goult2, E. C. Hardeman1, J. G. Lock1; 1University New South Wales, Sydney, AUSTRALIA, 2University of Kent, Canterbury, UNITED KINGDOM, 3Ludwig-Maximilians-University, Munich, GERMANY.
5:50 pm M56 Dynamically Heterogeneous Plasma Membrane Is Poised for Initiation of Receptor-mediated Mast Cell Signaling. N. Bag, D. Holowka, B. Baird; Cornell University, Ithaca, NY.
6:05 pm M57 Structural Organization of Caveolin-1 8S Oligomers Determined by Cryo-electron Microscopy. B. Han1, J. Porta2, E. Binshtein3, E. Karakas3, M. Ohi2, A. Kenworthy1; 1University of Virginia School of Medicine, Charlottesville, VA, 2University of Michigan Life Sciences Institute, Ann Arbor, MI, 3Vanderbilt University School of Medicine, Nashville, TN.
6:20 pm M58 Live-cell Imaging and Analysis of the Plasma Membrane Dynamics during Clathrin-mediated Endocytosis by High-speed Atomic Force Microscopy. A. Yoshida1, N. Sakai2, N. Takahashi1, S. H. Yoshimura3, Y. Ohba1; 1Department of Cell Physiology, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, Sapporo, JAPAN, 2R&D Group, OLYMPUS Corporation, Tokyo, JAPAN, 3Laboratory of Plasma Membrane and Nuclear Signaling, Graduate School of Biostudies, Kyoto University, Kyoto, JAPAN.
6:35 pm M59 Quantifying Cell Biology: Beyond Human Vision. A. E. Carpenter; Broad Institute of Harvard and MIT, Cambridge, MA.
Monday, December 9, 4:15 pm to 6:50 pm
Co-Chairs: Greg Alushin, Rockefeller University; and Ewa Paluch, University College of London
4:15 pm Introduction
4:20 pm M60 Modular Lim Domains Are Direct Sensors of Actin Strain to Mediate Mechanotransduction. X. Sun1, D. Phua1, L. Axiotakis, Jr.2, M. Smith3, A. Pasapera2, R. Gong1, R. Cail2, S. Espinosa1, C. Waterman2, M. Beckerle3, G. Alushin1; 1Rockefeller University, New York, NY, 2National Heart, Lung, and Blood Institute, NIH, Bethesda, MD, 3Huntsman Cancer Institute, University of Utah, Salt Lake City, UT.
4:35 pm M61 Identification of Context-specific Force-sensitive Protein Complexes within Focal Adhesions. A. Tao, A. LaCroix, B. Hoffman; Duke University, Durham, NC.
4:50 pm M62 Mechanical Force Promotes the Dissociation of Arp2/3 Complex Branches. N. G. Pandit, W. Cao, J. Bibeau, E. Johnson-Chavarria, T. D. Pollard, E. M. De La Cruz; Yale University, New Haven, CT.
5:05 pm M63 Pi-3 Kinase Drives Adaptive 3D Migration by Polarizing Large Pressure-based Protrusions. E. Welf, M. Driscoll, A. Weems, K. Dean, R. Fiolka, G. Danuser; UT Southwestern Medical Center, Dallas, TX.
5:20 pm M64 Coordination of Actomyosin Contractility and Mitochondrial Positioning during Interstitial Neutrophil Migration in Live Anesthetized Animal. N. Melis1, B. Subramanian1, D. Chen1, W. Losert2, C. Parent3, R. Weigert1; 1Laboratory of Cellular and Molecular Biology, Center for Cancer Research, National Cancer Institute – National Institutes of Health, Bethesda, MD, 2Department of Physics, Physical Sciences Complex, University of Maryland, College Park, MD, 3Department of Pharmacology, University of Michigan, Ann Arbor, MI.
5:35 pm M65 Mechanistic Insights Into Actin-based Force Generation during Clathrin-mediated Endocytosis via in Situ Cryo-Electron Tomography. D. Serwas1, M. Akamatsu1, K. M. Davies2, D. G. Drubin1; 1UC Berkeley, Berkeley, CA, 2Lawrence Berkeley National Laboratory, Berkeley, CA.
5:50 pm M66 Quantifying Non-muscle Myosin 2 Assembly Dynamics in 4D Super-resolution. M. A. Quintanilla1, H. Wu1, M. Akamatsu2, J. R. Beach1; 1Loyola University Chicago, Chicago, IL, 2University of California, Berkeley, Berkeley, CA.
6:05 pm M67 Actin Mechanics across Scales, from Molecular Processes to the Control of Cortical Tension. B. Truong Quang1, E. Paluch2, R. Peters2, D. Cassani1, G. Charras1; 1University College London, London, UNITED KINGDOM, 2University of Cambridge, Cambridge, UNITED KINGDOM.
6:20 pm M68 Short, Minimal Viral Fusogens Hijack the Actin Cytoskeleton to Drive Cell-cell Fusion. K. Chan1,2,3, S. Son2,3, E. M. Schmid2,3, A. L. Arthur3, A. Bhat3, J. Morstein3, D. Schlesinger3, D. A. Stevens3, D. A. Fletcher1,2,3; 1UC-Berkeley/UC-San Francisco Graduate Group in Bioengineering, Berkeley, CA, 2Department of Bioengineering, University of California, Berkeley, Berkeley, CA, 3Marine Biology Laboratory, Physiology Course, Woods Hole, MA.
6:35 pm M69 Signal Integration during Leukocyte Chemotaxis in Complex Microenvironments. P. J. Sáez1, M. Deygas1, I. Lavi1, M. Maurin2, M. Piel1, P. Vargas1; 1UMR144 Cell biology and Cancer Unit, Institut Curie, Paris, FRANCE, 2U932 Immunity and Cancer Unit, Institut Curie, Paris, FRANCE.
Co-Chairs: Tom Cheung, Hong Kong University of Science and Technology; and Prisca Liberali, Friedrich Miescher Institute for Biomedical Research
4:15 pm Introduction
4:20 pm M70 Systematic Analysis of the Connectivity and Dynamics of the MAPK Signaling Network Reveals Critical Nodes of Cross-regulation. A. F. Peterson, T. Aikin, S. Regot; Johns Hopkins University, Baltimore, MD.
4:35 pm M71 Epithelial Cells Act as a Regional Checkpoint for Immune Cell Organization. S. Park, C. Martone, V. Greco; Yale University, New Haven, CT.
4:50 pm M72 Stem Cell-Driven Lymphatic Remodeling Coordinates Tissue Regeneration. S. Gur-Cohen1, H. Yang1, S. C. Baksh1, Y. Miao1, J. Levorse1, R. P. Kataru2, X. Liu3, J. dela Cruz-Racelis1, B. J. Mehrara2, E. Fuchs1; 1Rockefeller University, New York, NY, 2Memorial Sloan Kettering Cancer Center, New York, NY, 3Northwestern University, Chicago, IL.
5:05 pm M73 Unraveling the Gene Regulatory Network of Early Lineage Commitment and Context-Dependent TGF-beta Response. J. Valcourt1, R. E. Huang1, S. Kundu2, R. E. Kingston3, S. Ramanathan1; 1Harvard University, Cambridge, MA, 2Intellia Therapeutics, Cambridge, MA, 3Massachusetts General Hospital, Boston, MA.
5:20 pm M74 Physical Mechanisms for Oncogene-induced Breakdown in Mammary Tissue Structure during Cancer Progression. V. Srivastava1, J. L. Hu1, J. C. Garbe2, M. R. Stampfer2, M. A. LaBarge3, Z. J. Gartner1; 1University of California San Francisco, San Francisco, CA, 2Lawrence Berkeley National Laboratory, Berkeley, CA, 3Beckman Research Institute, City of Hope, Duarte, CA.
5:35 pm M75 Intracellular Ph Dynamics Regulates Intestinal Stem Cell Differentiation. Y. Liu, T. Nystul, D. Barber; UCSF, San Francisco, CA.
5:50 pm M76 Modeling Human Skeletal Muscle Development and Stem Cell Niche Formation in Vivo. M. Hicks, M. Yang, S. Younesi, H. Xi, A. Pyle; UCLA, Los Angeles, CA.
6:05 pm M77 Tension Suppresses Epithelial Stem Cell Differentiation. M. King1, R. Stewart1, A. Zubek2, E. Carley1, A. Zieman2, V. Horsley2; 1Yale School of Medicine, New Haven, CT, 2Yale University, New Haven, CT.
6:20 pm M78 dek Modulates Global Intron Retention to Control Quiescence Exit in Muscle Stem Cells. T. H. Cheung; Hong Kong University of Science and Technology, Hong Kong, HONG KONG.
6:35 pm M79 Regenerative Landscape of Intestinal Organoids. P. Liberali; Friedrich Miescher Institute, Basel, SWITZERLAND.
Co-Chairs: Francesca Giordano, Institut de Biologie Intégrative de la Cellule (I2BC); and Amy Kiger, University of California, San Diego
4:15 pm Introduction
4:20 pm M80 The Nuclear Envelope Enriched Lipin Phosphatase Ctdnep1 Links Misregulation of Lipid Metabolism to Chromosome Instability in Dividing Cancer Cells. H. Merta, J. Carrasquillo-Rodriguez, M. Deline, T. Vitale, S. Bahmanyar; Yale University, New Haven, CT.
4:35 pm M81 Defining the Subcellular Distribution and Metabolic Channeling of Phosphatidylinositol. J. G. Pemberton1, Y. Kim1, N. Sengupta1, A. Eisenreichova2, D. J. Toth1, E. Boura2, T. Balla1; 1National Institute of Child Health and Human Development, NIH, Bethesda, MD, 2Czech Academy of Sciences, Prague, CZECH REPUBLIC.
4:50 pm M82 LetB Forms a Tunnel for Lipid Transport across the Bacterial Periplasm. D. C. Ekiert; New York University School of Medicine, New York, NY.
5:05 pm M83 Dual Role of Orp5 in Regulating Lipid Transport and Calcium Import to Mitochondria at Endoplasmic Reticulum (ER)- Mitochondria Membrane Contact Sites. L. Rochin1, F. Giordano1, C. Sauvanet1, E. Jääskeläinen2, A. Houcine3, A. Kivela2, X. MA4, E. Marien5, J. Dehairs5, J. Neveu1, R. Le Bars1, J. Swinnen5, D. Bernard4, D. Tareste6, V. Olkkonen7; 1Institut de Biologie Integrative de la Cellule (I2BC)-UMR9198/CNRS, Gif-sur-Yvette, FRANCE, 2Minerva Foundation Institute for Medical Research, Biomedicum 2U, Helsinki, FINLAND, 3Institut Jacques Monod, CNRS, UMR7592, Paris, FRANCE, 4Centre de Recherche en Cancérologie de Lyon, UMR INSERM U1052/CNRS 5286, Lyon, FRANCE, 5Laboratory of Lipid Metabolism and Cancer, Department of Oncology, KU Leuven, Leuven, BELGIUM, 6Institute of Psychiatry and Neuroscience of Paris (IPNP), UMR-S1266 INSERM, Paris, FRANCE, 7Department of Anatomy, Faculty of Medicine, FI-00014 University of Helsinki, Helsinki, FINLAND.
5:20 pm M84 GRAMD Proteins Localize to Multiple Membrane Contact Sites and Regulate Plasma Membrane Cholesterol Homeostasis. Y. Saheki1, T. Naito1, Y. Saheki2, B. Ercan1, L. Krshnan1, A. Triebl3, D. Koh1, F. Wei4, K. Tomizawa4, F. Torta3, M. Wenk3; 1Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore, SINGAPORE, 2Institute of Resource Development and Analysis, Kumamoto University, Kumamoto, JAPAN, 3Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, SINGAPORE, 4Department of Molecular Physiology, Faculty of Life Sciences, Kumamoto University, Kumamoto, JAPAN.
5:35 pm M85 Loss of Lysosomal Npc1 Function Increases PtdIns4P to Sustain ER to Golgi Cholesterol Transfer. O. Vivas, C. Kutchukian, M. Casas Prat, J. G. Jones, S. A. Tiscione, R. Dixon, E. J. Dickson; University of California, Davis, Davis, CA.
5:50 pm M86 RASSF4 Functions at the Interface Between Actin and Intracellular Vesicles to Regulate PI(4,5)P2 Synthesis. Y. Chen, D. Ryskamp, I. Bezprozvanny, J. Liou; UT Southwestern at Dallas, Department of Physiology, TX.
6:05 pm M87 A PI3KC2 Noncatalytic Splice Variant Regulates Phosphoinositide Activities in Endolysosomal Trafficking. A. A. Kiger, J. Groulx, S. Jean, C. Bao; University of California, San Diego, La Jolla, CA.
6:20 pm M88 The Lipid Kinase, Vps34, Is a Driver of Selected Membrane Trafficking Pathways. N. Steinfeld, V. Lahiri, A. Morrison, D. J. Klionsky, L. S. Weisman; University of Michigan, Ann Arbor, MI.
6:35 pm M89 Rab3 Is a Key Player in Microdomain-dependent Plasma Membrane Recycling. B. Diaz-Rohrer1, K. Levental2, I. Levental2; 1University of Texas Hlth Sci Ctr Houston, Houston, TX, 2UT Health Science Center at Houston, Houston, TX.
Co-Chairs: Jonathan Chubb, University College of London; and Tracy Johnson, University of California, Los Angeles
4:15 pm Introduction
4:20 pm M90 Origins and Implications of Transcriptional Bursts. J. Chubb; University College London, London, UNITED KINGDOM.
4:35 pm M91 Transcriptional Bursting at a Single Promoter Level Is Regulated by Transcription Factor Mobility and Hormone Release Pattern. D. Stavreva1, D. Garcia2, G. Fettweis1, A. McGowen1, M. Ferguson3, A. Upadhyaya2, G. Hager1; 1NCI, NIH, Bethesda, MD, 2University of Maryland, College Park, MD, 3Boise State University, Boise, ID.
4:50 pm M92 RNA Splicing, Chromatin Modification, and the Coordinated Control of Gene Expression. T. Johnson; UCLA, Los Angeles, CA.
5:05 pm M93 Availability of Splicing Factors in the Nucleoplasm Can Regulate the Release of Mrna From the Gene After Transcription. Y. Shav-Tal, H. Hochberg-Laufer, N. Neufeld, Y. Brody; Bar-Ilan University, Ramat Gan, ISRAEL.
5:20 pm M94 The Midbody Is a Novel Translating Organelle Mediating Intercellular Communication. R. Dahn1, S. Park1, E. Kurt1, A. Presle2, J. Gilbert1, K. VanDenHeuvel1, A. Jambhekar3, J. Shivas4, L. Qin1, O. Olukoga1, A. Echard2, M. Blower5, A. R. Skop1; 1University Wisconsin-Madison, Madison, WI, 2Instit Pasteur, Paris, FRANCE, 3Harvard, Boston, WI, 4Leica Microsystems, Buffalo Grove, IL, 5Harvard, Boston, MA.
*5:35 pm M95 Cross-kingdom Recognition of Bacterial Small RNAs Induces Transgenerational Pathogenic Avoidance. C. T. Murphy; Princeton University, Princeton, NJ.
5:50 pm M96 Transcription Factor Foxa1 Clusters DNA through Wetting-mediated Capillary Forces. T. Quail, S. Golfier, D. Oriola, J. Brugues; Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, GERMANY.
6:05 pm M97 Confined Migration Induces Heterochromatin Formation in Cancer Cells. C. Hsia1,2, O. Hasan2, C. Chang2,3, J. Lammerding1,2,3; 1Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY, 2Weill Institute for Cell and Molecular Biology, Cornell University, Ithaca, NY, 3Nancy E. and Peter C. Meinig School of Biomedical Engineering, Cornell University, Ithaca, NY.
6:20 pm M98 Identification of the Cellular Determinants of Stochastic Transcriptional Bursting. V. Sood, Y. Wan, G. Pegoraro, P. Gudla, D. Larson, T. Misteli; National Cancer Institute, NIH, Bethesda, MD.
**6:35 pm M99 Structure and Function of Mammalian Swi/snf Chromatin Remodeling Complexes in Human Cancer. C. Kadoch; Dana-Farber Cancer Institute and Harvard Medical School, Boston, MA.
*WICB Junior Awardee for Excellence in Research
**ASCB Early Career Awardee
Co-Chairs: Sadie Wignall, Northwestern University; and Jennifer DeLuca, Colorado State University
4:15 pm Introduction
4:20 pm M100 Cryo-ET Analysis of the Yeast Synaptonemal Complex in Situ. L. Gan, O. X. Ma, S. Cai, J. Shi; National University of Singapore, Singapore, SINGAPORE.
4:35 pm M101 CDK-2 Mediates Crossover Designation through Phosphorylation of the Mutsγ Complex. J. Haversat1, A. Woglar2, K. Klatt1, V. Roberts1, S. Arur3, A. M. Villeneuve2, Y. Kim1; 1Johns Hopkins University, Baltimore, MD, 2Stanford University, Stanford, CA, 3M.D. Anderson Cancer Center, Houston, TX.
4:50 pm M102 Mechanisms of Acentrosomal Spindle Assembly and Maintenance in C. Elegans Oocytes. I. D. Wolff, S. M. Wignall; Northwestern University, Evanston, IL.
5:05 pm M103 Non-overlapping Functions of Aurkb and Aurkc in Regulating the Spindle Assembly Checkpoint during Oocyte Meiosis. C. S. Blengini, A. L. Nguyen, G. Jung, K. Schindler; Rutgers University, New Brunswick, Piscataway, NJ.
5:20 pm M104 Tetraploidy Causes Chromosomal Instability in Acentriolar Mouse Embryos. L. Gomes Paim1, G. FitzHarris1,2; 1Centre de Recherche du Centre Hospitalier de l’Université de Montréal, Montréal, QC, CANADA, 2Département d’Obstétrique-Gynécologie, Université de Montréal, Montréal, QC, CANADA.
5:35 pm M105 A Molecular Mechanism for Assembly of the Pre-procentriole. T. A. McLamarrah1, G. C. Rogers1, J. M. Ryniawec1, S. Speed2, D. W. Buster1, C. J. Fagerstrom2, B. J. Galletta2, N. M. Rusan2; 1University of Arizona, Tucson, AZ, 2National Institutes of Health, Bethesda, MD.
5:50 pm M106 Crispr-cas9-based Tiling Screens Reveal Protein Domain Structure and Novel Functional Regions in Human Mitotic Factors. J. Herman, L. Carter, S. Biggins, P. Paddison; Fred Hutchinson Cancer Research Center, Seattle, WA.
6:05 pm M107 Aurora B Kinase Is Recruited to Multiple Discrete Kinetochore and Centromere Regions in Human Cells. J. G. DeLuca, A. J. Broad, K. F. DeLuca; Colorado State University, Fort Collins, CO.
6:20 pm M108 Cellular Limits on the Anaphase Spindle Elongation Rate Ensure That Chromosomes Are Dynamically Disentangled during Cell Division. S. Mukherjee1, D. Tank1, E. Davidson2, M. McClellan1, T. Davis2, M. K. Gardner1; 1University of Minnesota, Minneapolis, MN, 2University of Washington, Seattle, WA.
6:35 pm M109 Abscission Couples Cell Division to Exit from Pluripotency. A. Chaigne1, M. B. Smith2, C. Labouesse3, E. Hannezo4, K. J. Chalut3, E. K. Paluch5; 1MRC/LMCB, University College London, London, UNITED KINGDOM, 2The Francis Crick Institute, London, UNITED KINGDOM, 3Stem Cell Institute, University of Cambridge, Cambridge, UNITED KINGDOM, 4IST Austria, Klosterneuburg, AUSTRIA, 5Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge, UNITED KINGDOM.
Co-Chairs: Cassandra Extavour, Harvard University; and Hong-Wei Wang, Tsinghua University
4:15 pm Introduction
4:20 pm M110 Applying Genetic Code Expansion and Bioorthogonal Labeling to Quantitative High-resolution Live Cell Imaging. N. Elia, A. Koening, I. Segal; Ben Gurion University of the Negev, Beer Sheva, ISRAEL.
4:35 pm M111 Towards “Autonomous” Microscopy: Artificial Intelligence Microscopy Screening (AIMS). G. Kanfer1, Y. Maman2, S. Sarraf1, H. Baldwin1, J. Lippincott-Schwartz3, R. Youle1; 1Biochemistry Section, Surgical Neurology Branch, National Institute of Neurological Disorders and Stroke, NIH, Bethesda, MD, 2Laboratory of Genome Integrity, National Cancer Institute, NIH, Bethesda, MD, 3Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA.
4:50 pm M112 Live Simultaneous Multi-receptor Tracking in Neurons with 5-D Single Molecule Localization Microscopy. C. Butler1,2, G. E. Saraceno1,2, M. A. Kechkar3, V. Studer1, L. Groc1,2, R. Galland1,2, J. Sibarita1; 1University of Bordeaux, Interdisciplinary Institute for Neuroscience, Bordeaux, FRANCE, 2CNRS UMR5297, Bordeaux, FRANCE, 3Ecole Nationale Supérieure de Biotechnologie, Constantine, ALGERIA.
5:05 pm M113 High Speed Single Molecule Dynamics in the Endoplasmic Reticulum. C. J. Obara1, J. Nixon-Abell2, F. Riccio2, C. Blackstone2, J. Lippincott-Schwartz, 201471; 1HHMI Janelia Research Campus, Ashburn, VA, 2NINDS\NIH, Bethesda, MD.
5:20 pm M114 Engineering Biologically-relevant Boundaries: Optochemical Patterning of a Synthetic Cell Boundary to Probe Spindle Positioning. J. G. Bermudez, M. C. Good; University of Pennsylvania, Philadelphia, PA.
5:35 pm M115 Blastoderm Formation in Crickets: A Crowding Mechanism Explains Nucleus Speed, Direction, and Division Timing. S. Donoughe1, J. Hoffmann2, T. Nakamura3, C. H. Rycroft2,4, C. G. Extavour2; 1University of Chicago, Chicago, IL, 2Harvard University, Cambridge, MA, 3National Institute for Basic Biology, Okazaki, JAPAN, 4Lawrence Berkeley Laboratory, Berkeley, CA.
5:50 pm M116 Location-dependent Noncanonical Elemental Composition and Cellular Architecture of the Statolith and Underlying Cells in the Early-diverging Metazoans, Beroe Ovata and Mnemiopsis Leidyi. A. G. Moss, W. Hames; Auburn University, Auburn, AL.
6:05 pm M117 A New Window Into the Evolution of Fungal and Animal Cell Biology: Genetic Transformation of the Chytrid Spizellomyces. E. M. Medina1, K. A. Robinson1, L. K. Fritz-Laylin1, N. E. Buchler2; 1University of Massachusetts, Amherst, MA, 2North Carolina State University, Raleigh, NC.
6:20 pm M118 Dissecting the Role of Innate Immunity for Symbiont Tolerance in Coral Endosymbiosis. A. Guse, M. R. Jacobovitz, S. Rupp; COS, Heidelberg University, Heidelberg, GERMANY.
6:35 pm M119 Scale-free Tracking Microscopy of Freely Suspended Cells: Towards Connecting Cell Biology and Ocean Ecology. D. Krishnamurthy1, H. Li1, A. Larson1, D. Mion2, E. Li1, F. B. D. Rey2, M. Prakash1; 1Stanford University, Stanford, CA, 2Ecole Polytechnique, Paris, FRANCE.
Tuesday, December 10, 4:15 pm to 6:50 pm
Co-Chairs: Martin Graef, Max Planck Institute for Biology of Ageing, Cologne; and Roberto Zoncu, University of California, Berkeley
4:15 pm Introduction
4:20 pm M120 Molecular Mechanisms of the Mitochondrial Motors of Mass Destruction. G. Lander1, C. Puchades1, M. Shin1, S. Glynn2, W. Karzai2; 1Scripps Research, La Jolla, CA, 2Stony Brook University, Stony Brook, NY.
4:35 pm M121 Novel Translation Repression Complex Prevents Ribosome Initiation on Faulty Messenger RNAs. K. Hickey1, K. Kostova2, J. Replogle1, K. Disckson3, K. D'Orazio4, N. Sinha4, R. Green4, J. Weissman1; 1University of California, San Francisco, San Francisco, CA, 2Carnegie Inst Washington, Baltimore, MD, 3Lawrence University, Appletown, WI, 4Johns Hopkins School of Medicine, Baltimore, MD.
4:50 pm M122 Activated Ire1 Oligomerizes Into Filaments Contained in Anastomosing 30 Nm Endoplasmic Reticulum Membrane Tubes. N. Tran1,2, S. Carter3,2, V. Belyy1,2, D. Acosta-Alvear4, G. Jensen3,2, P. Walter1,2; 1University of California San Francisco, San Francisco, CA, 2Howard Hughes Medical Institute, Chevy Chase, MD, 3California Institute of Technology, Pasadena, CA, 4University of California Santa Barbara, Santa Barbara, CA.
5:05 pm M123 ER Stress Response in a Premature Aging Disease. S. Vidak, L. Serebryannyy, T. Misteli; NCI/NIH, Bethesda, MD.
5:20 pm M124 In Cellulo Structure of the Nuclear Pore Complex and Its Implications in Mrna Export and Nuclear Pore Turnover by Autophagy. M. Allegretti1, C. E. Zimmerli1, V. Rantos2, P. Ronchi1, F. Wilfling3, K. H. Fung1, C. Lee3, Y. Schwab1, J. Mahamid1, B. Pfander3, J. Kosinski2, M. Beck1; 1EMBL Heidelberg, Heidelberg, GERMANY, 2EMBL Hamburg, Hamburg, GERMANY, 3Max Planck Institute of Biochemistry, Munich, GERMANY.
5:35 pm M125 Uncovering Cellular Mechanisms Controlling Tau Aggregation and Toxicity Using CRISPRi-based Genome-wide Screens in Human Neurons. M. Kampmann, A. Samelson, G. Mohl, R. Tian; University of California, San Francisco, San Francisco, CA.
5:50 pm M126 System-Wide Profiling of Mitotic Ageing Reveals Pro-Ageing Functions of the Autophagy Machinery. M. Graef; Max Planck Institute for Biology of Ageing, Cologne, Cologne, GERMANY.
6:05 pm M127 Selective Autophagic Clearance of Neurodegeneration-associated Protein Aggregates Is Mediated by the Autophagy Receptor, TAX1BP1. S. A. Sarraf1, H. V. Shah1,2, G. Kanfer1, A. M. Pickrell3, L. A. Holtzclaw4, M. E. Ward1, R. J. Youle1; 1Biochemistry Section, Surgical Neurology Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD, 2University of Maryland, College Park, MD, 3School of Neuroscience Science, College of Science, Virginia Tech, Blacksburg, VA, 4Microscopy and Imaging Core, Office of the Scientific Director, Intramural Research Program, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD.
6:20 pm M128 Synaptic Activity Regulates Local Autophagy in Dendrites of Primary Neurons. S. Maday, V. V. Kulkarni, A. Anand, J. Brandt; Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA.
6:35 pm M129 Regulation of Cholesterol-dependent Mtorc1 Signaling by Inter-organelle Contacts. C. Lim1,2, O. Davis1,2, H. Shin1,2, J. Zhang1,2, C. Berdan1,3, X. Jiang4, J. Counihan1,3, D. Ory4, D. Nomura1,3, R. Zoncu1,2; 1Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, CA, 2The Paul F. Glenn Center for Aging Research at the University of California, Berkeley, Berkeley, CA, 3Department of Nutritional Sciences and Toxicology, University of California at Berkeley, Berkeley, CA, 4Diabetic Cardiovascular Disease Center, Washington University School of Medicine, St Louis, MO.
Co-Chairs: Dan Jarosz, Stanford University; and Jeff Woodruff, University of Texas, Southwestern Medical Center
4:15 pm Introduction
4:20 pm M130 ER Membranes Exhibit Phase Behavior at Sites of Organelle Contact. C. King, P. Sengupta, A. Seo, J. Lippincott-Schwartz; HHMI Janelia Research Campus, Ashburn, VA.
4:35 pm M131 Protein Phase Separation as a Membrane Curvature Sensing Switch. G. Kago, F. Yuan, W. F. Zeno, J. C. Stachowiak; University of Texas at Austin, Austin, TX.
4:50 pm M132 Ph-triggered Coacervate Formation Activates Enzyme Reactions. C. Love1, J. Steinkühler2, R. Dimova2, D. Tang1; 1Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, GERMANY, 2Max Plank Institute of Colloids and Interfaces, Potsdam, GERMANY.
5:05 pm M133 Proteome-wide Analysis of Cytoplasmic Meso-scale Organization. F. Keber, M. Wühr, C. Brangwynne; Princeton University, Princeton, NJ.
5:20 pm M134 The Transition Into and Out of Glucose Starvation Induced Cytoplasmic Freezing in Fission Yeast. A. Foote, M. Williamson, E. Florin; University of Texas at Austin, Austin, TX.
5:35 pm M135 The Properties of Membraneless Organelles Are Tuned to Environmental Conditions. B. Stormo1, F. Dietrich2, C. Roden1, A. Gladfelter1; 1University of North Carolina-Chapel Hill, Chapel Hill, NC, 2Duke University, Durham, NC.
5:50 pm M136 Adaptive Control of Cell Division Programs by Pervasive Protein Self-assembly. D. F. Jarosz; Stanford University, Stanford, CA.
6:05 pm M137 Mitochondrial Nucleoids Self-assemble via Phase Separation. M. Feric1,2, T. G. Demarest3, J. Tian3, D. L. Croteau3, V. A. Bohr3, T. Misteli2; 1NIGMS, Bethesda, MD, 2NCI, Bethesda, MD, 3NIA, Baltimore, MD.
6:20 pm M138 Robust Modulation of Kinase Activity within a Bacterial Biomolecular Condensate. S. Saurabh, L. Shapiro; Stanford University, Stanford, CA.
6:35 pm M139 Material Aging Underlies Centrosome Weakening and Disassembly during Mitotic Exit. J. Woodruff; UT Southwestern Medical Center, Dallas, TX.
Co-Chairs: Sophie G. Martin, University of Lausanne; and Jeremy Nance, New York University School of Medicine
4:15 pm Introduction
4:20 pm M140 An Interphase Contractile Ring Reshapes Primordial Germ Cells to Allow Bulk Cytoplasmic Remodeling. J. Nance; New York University School of Medicine, New York, NY.
4:35 pm M141 Leading Edge Maintenance in Migrating Neutrophil-like HL-60 Cells Is an Emergent Property of Branched Actin Growth. R. M. Garner1, E. F. Koslover2, A. J. Spakowitz1, J. A. Theriot3; 1Stanford University, Stanford, CA, 2University of California San Diego, San Diego, CA, 3Howard Hughes Medical Institute, University of Washington, Seattle, WA.
4:50 pm M142 Negative Surface Charge Defines the State of Cell Cortex and Regulates Excitable Dynamics in Amoeboid Migration and Macropinocytosis. T. Banerjee1, D. Biswas2, D. S. Pal1, Y. Miao1, P. A. Iglesias2, P. N. Devreotes1; 1Department of Cell Biology, Johns Hopkins University School of Medicine, Baltimore, MD, 2Department of Electrical and Computer Engineering, Johns Hopkins University, Baltimore, MD.
5:05 pm M143 Clics Are Ancient Conserved Regulators of Gα12/13 and Rac Signaling in Angiogenesis and Tubulogenesis. A. Arena, D. Shaye, D. Mao, J. Kitajewski; Dept. of Physiology and Biophysics. University of Illinois at Chicago, Chicago, IL.
5:20 pm M144 A Template for Actin Organization at the Leading Edge. A. Pipathsouk, R. Brunetti, J. Town, O. Weiner; University of California, San Francisco, San Francisco, CA.
5:35 pm M145 Patterning of Membrane-associated Proteins through Membrane Flows. V. Gerganova1, I. Lamas1, D. Rutkowski2, A. Vjestica1, D. Vavylonis2, S. G. Martin1; 1University of Lausanne, Lausanne, SWITZERLAND, 2Lehigh University, Bethlehem, PA.
5:50 pm M146 An Asymmetric Mechanoresponse at Cadherin Junctions Ensures Epithelial Integrity during Mitotic Rounding. M. Gloerich1, J. Monster1, L. Donker1, M. Vliem1, Z. Win2, J. De Rooij1, B. Baum2; 1UMC Utrecht, Center for Molecular Medicine, Utrecht, NETHERLANDS, 2UCL, London, UNITED KINGDOM.
6:05 pm M147 CD2AP Links Actin to PI3 Kinase Activity to Extend Epithelial Cell Height and Constrain Cell Area. Y. Wang, W. M. Brieher; Department of Cell and Developmental Biology, University of Illinois, Urbana-Champaign, IL.
6:20 pm M148 MAPK Feedback Phosphorylation of RGS Controls Its Spatiotemporal Localization and Alters Endocytosis through the Kelch Repeat Protein Kel1 during the Yeast Pheromone Response. W. C. Simke, J. B. Kelley, A. J. Hart; University of Maine, Orono, ME.
6:35 pm M149 RhoA Mediates Epithelial Cell Shape Changes via Mechanosensitive Endocytosis. K. Cavanaugh1,2, M. Staddon3, E. Munro1,4, S. Banerjee3, M. Gardel4,5; 1Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, IL, 2Committee on Development, Regeneration and Stem Cell Biology, University of Chicago, Chicago, IL, 3Department of Physics and Astronomy, and Institute for the Physics of Living Systems, University College London, London, UNITED KINGDOM, 4Institute for Biophysical Dynamics, University of Chicago, Chicago, IL, 5James Franck Institute, and Department of Physics, University of Chicago, Chicago, IL.
Co-Chairs: Marija Zanic, Vanderbilt University; and Anthony Roberts, University of London
4:15 pm Introduction
4:20 pm M150 A Structural and Mechanistic Model for the Regulation of LRRK2’S Interaction with Microtubules. J. Salogiannis; University of California San Diego, San Diego, CA.
4:35 pm M151 Mitochondria-adaptor TRAK Enables Kinesin-1 Driven Transport in Crowded Environments. V. Henrichs1,2, Z. Nahácka1, J. Rohlena1, C. Bařinka1, J. Neužil1, S. Diez3,4, M. Braun1, Z. Lánský1; 1Institute of Biotechnology of the Czech Academy of Sciences, BIOCEV, Vestec u Prahy, CZECH REPUBLIC, 2Faculty of Science, Charles University in Prague, Prague, CZECH REPUBLIC, 3B CUBE - Center of Molecular Bioengineering, Technische Universität Dresden, Dresden, GERMANY, 4Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, GERMANY.
4:50 pm M152 Structure of the Dynein-2 Complex and Its Assembly with Intraflagellar Transport Trains. K. Toropova1, A. J. Roberts1, R. Zalyte2, A. G. Mukhopadhyay1, M. Mladenov1, A. Carter2; 1Birkbeck, University of London, London, UNITED KINGDOM, 2Medical Research Council Laboratory of Molecular Biology, Cambridge, UNITED KINGDOM.
5:05 pm M153 Physical Integration within Xenopus Egg Extract Microtubule Asters Suggests Aster Movement Is Driven by Dynein-dependent Surface Forces and Actomyosin Contraction. J. Pelletier1,2,3, C. Field1,2, S. Fürthauer4, N. Fakhri3, T. Mitchison1,2; 1Harvard Medical School, Boston, MA, 2Marine Biological Laboratory, Woods Hole, MA, 3Massachusetts Institute of Technology, Cambridge, MA, 4Flatiron Institute, New York, NY.
*5:20 pm M154 How to Make Microtubules and Build the Mitotic Spindle. S. Petry; Princeton University, Princeton, NJ.
5:35 pm M155 The Mitotic Spindle Protein Ckap2 Is a Potent Microtubule Assembly Factor. T. McAlear, S. Bechstedt; McGill University, Montreal, QC, CANADA.
5:50 pm M156 Multi-component in Vitro Reconstitution Induces Robust Microtubule Treadmilling. M. Zanic, G. Arpag, E. J. Lawrence; Vanderbilt University, Nashville, TN.
6:05 pm M157 Reconstitution of Dynamic Actin Cables from Purified Components. L. W. Pollard, M. V. Garabedian, S. L. Alioto, B. L. Goode; Brandeis University, Waltham, MA.
6:20 pm M158 Cell Division Proteins Follow Treadmilling FtsZ Filaments by Diffusion-and-Capture. N. Baranova1, M. Loose1, P. Radler1, V. M. Hernández-Rocamora2, W. Vollmer2; 1Institute of Science and Technology Austria, Klosterneuburg, AUSTRIA, 2Newcastle University, Newcastle, UNITED KINGDOM.
6:35 pm M159 Phosphoinositides Regulate Force-independent Interactions between Talin, Vinculin, and Actin in Vitro. C. Kelley, T. Litschel, D. Dedden, S. Schumacher, P. Schwille, N. Mizuno; Max Planck Institute of Biochemistry, Martinsried, GERMANY.
*WICB Junior Awardee for Excellence in Research
Co-Chairs: Caren Norden, Max Planck Institute of Molecular Cell Biology and Genetics; and Angelike Stathopoulos, California Institute of Technology
4:15 pm Introduction
4:20 pm M160 The Role of Cell and Tissue Morphology in Neuroepithelial Nuclear Positioning. C. Norden1,2; 1MPI-CBG, Dresden, GERMANY, 2Instituto Gulbenkian de Ciência, Oeiras, PORTUGAL.
4:35 pm M161 An Adhesion Code Enables Robust Pattern Formation in the Zebrafish Spinal Cord. T. Tsai1, M. Sikora2, C. Heisenberg2, S. Megason1; 1Harvard Medical School, Boston, MA, 2Institute of Science and Technology, Klosterneuberg, AUSTRIA.
4:50 pm M162 Differentiation of Structurally- and Optically- Distinct Types of Iridophores Is Required for Stripe Formation in Zebrafish. D. Gur1,2, E. Bain3, D. Parichy3, J. Lippincott-Schwartz2; 1NIH/NICHD, Bethesda, MD, 2Janelia Research Campus, Ashburn, VA, 3University of Virginia, Charlottesville, VA.
5:05 pm M163 FGF Signaling Controls Epithelial-mesenchymal Transition during Gastrulation through the Regulation of Cell Adhesion and Division. J. Sun, V. A. Stepanik, A. Stathopoulos; Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA.
5:20 pm M164 Acute Rho1 Activation Reveals That Ventral Epithelial Cells of the Drosophila Embryo Are Specifically Predisposed for Coordinated Anisotropic Constriction during Gastrulation. A. Rich, R. Fehon, M. Glotzer; University of Chicago, Chicago, IL.
5:35 pm M165 Distinct Prepatterns of RhoA Activity and F-actin Levels Promote Tissue Folding. M. Denk-Lobnig, N. C. Heer, A. C. Martin; MIT, Cambridge, MA.
5:50 pm M166 A Single-cell Reconstruction of Planarian Regeneration Identifies Wound-induced Transcriptional States Required for Tissue Repair. B. Benham-Pyle1, C. Brewster1, A. Kent1, S. Chen1, F. Mann1,2, A. Scott1, A. Box1, A. Sánchez Alvarado1,2; 1Stowers Institute for Biomedical Research, Kansas City, MO, 2Howard Hughes Medical Institute, Chevy Chase, MD.
6:05 pm M167 Simple Geometric Rules Define the Shape and Stability of Epithelial Lumens. C. G. Vasquez, V. T. Vachharajani, A. R. Dunn; Stanford University, Stanford, CA.
6:20 pm M168 Cells in Intermediate States during EMT Are Characterized by Specific Geometrical and Mechanical Intra-cellular Architectures. Y. Margaron1, L. Guyon2, L. Kurzawa3, A. Morel4, A. Pinhiero5, L. Blanchoin3, F. Reyal5, A. Puisieux4, M. Thery6; 1CytoMorpho Lab, IRIG, Paris, FRANCE, 2Interdisciplinary Research Institute of Grenoble (IRIG), Grenoble, FRANCE, 3CytoMorpho Lab, IRIG, Grenoble, FRANCE, 4Cancer Research Center of Lyon, Lyon, FRANCE, 5RT2Lab, PSL Research University, Paris, FRANCE, 6CytoMorpho Lab, Hopital Saint Louis, Paris, FRANCE.
6:35 pm M169 Membrane Tension Regulates Fgf Driven Fate Choice in Embryonic Stem Cells. H. De Belly1, P. H. Jones2, K. J. Chalut3, E. K. Paluch4,1; 1MRC Laboratory for Molecular Cell Biology, University College London, London, UNITED KINGDOM, 2Department of Physics & Astronomy, University College London, London, UNITED KINGDOM, 3Wellcome Trust/Medical Research Council Cambridge Stem Cell Research Institute, Cambridge, UNITED KINGDOM, 4Department of Physiology, Development and Neuroscience, Cambridge, UNITED KINGDOM.
Co-Chairs: Emily Hatch, Fred Hutchinson Cancer Research Center; and Dan Levy, University of Wyoming
4:15 pm Introduction
4:20 pm M170 Cytoplasmic Volume and Limiting Nucleoplasmin Scale Nuclear Size during Xenopus Laevis Development by Altering Chromatin Organization. P. Chen, M. Tomschik, K. Nelson, J. Oakey, J. C. Gatlin IV, D. L. Levy; University of Wyoming, Laramie, WY.
4:35 pm M171 Prostaglandins Restrict Nuclear Actin to Control the Nucleolus. D. Wineland1, T. Tootle1, G. Kimble1, D. Kelpsch2; 1University of Iowa-Carver Coll Med, Iowa City, IA, 2Carnegie Institution for Science, Baltimore, MD.
4:50 pm M172 Examining the Modular Architecture of the Nuclear Pore Complex through Targeted Degradation. S. Regmi, R. Kaufhold, V. Aksenova, S. Chen, H. Lee, E. Turcotte, A. Arnaoutov, M. Dasso; National Institutes of Health, Bethesda, MD.
5:05 pm M173 The Mechanosensitivity of Nucleocytoplasmic Transport Is Governed by Increased Active Transport Both Into and Out of the Nucleus. I. Andreu1, I. Granero-Moya1, M. Molina1, V. González-Tarragó1, J. Kechagia1, P. Roca-Cusachs1,2; 1IBEC, Barcelona, SPAIN, 2UB and Ciber, Barcelona, SPAIN.
5:20 pm M174 The Giant KASH Protein ANC-1 Functions with and Without LINC Complexes to Position Nuclei and Other Organelles. H. Hao, S. Kalra, L. Jameson, L. Herrera, N. Cain, D. Starr; University California-Davis, Davis, CA.
5:35 pm M175 Nuclear Membrane Stability in Micronuclei Determined by Chromatin Content. H. Z. Huang, E. M. Hatch, E. M. Choo; Fred Hutchinson Cancer Research Center, Seattle, WA.
*5:50 pm M176 Structure-function Analysis of Heh1(LEM2) and Chm7 Suggests Role for Direct-PA-binding in Nuclear Envelope Surveillance. D. J. Thaller1, C. J. Marklew2, B. Ciani2, C. P. Lusk1; 1Yale School of Medicine, New Haven, CT, 2University of Sheffield, Sheffield, UNITED KINGDOM.
6:05 pm M177 Maternal and Paternal Genome Mixing in the C. Elegans Zygote Involves Stepwise Pronuclear Fusion and Fenestration of Pronuclear Membranes. M. M. Rahman1, A. Harned2, I. Chang2, R. Maheshwari1, K. Narayan2, O. Cohen-Fix1; 1National Institute of Diabetes and Digestive and Kidney Diseases, NIH, Bethesda, MD, 2Frederick National Laboratory for Cancer Research, NCI, NIH, Frederick, MD.
6:20 pm M178 Mutant Lamins Cause Nuclear Envelope Rupture and DNA Damage in Skeletal Muscle Cells. T. Kirby1, A. Earle1, G. Fedorchak1, P. Isermann1, J. Patel1, S. Iruvanti1, S. Moore2, G. Bonne3, L. Wallrath2, J. Lammerding1; 1Cornell University, Ithaca, NY, 2University of Iowa, Iowa City, IA, 3Sorbonne Université, Center of Research in Myology, Association Institute of Myology, Paris, FRANCE.
6:35 pm M179 Phase-separated Heterochromatin Domains Impart Mechanical Stiffness to the Nucleus. M. C. King1, J. F. Williams1, I. V. Surovtsev1, A. Nguyen2, S. G. J. Mochrie3; 1Yale School of Medicine, New Haven, CT, 2Pomona College, Claremont, CA, 3Yale University, New Haven, CT.
*ASCB Porter Prize Awardee for Research Excellence
Wednesday, December 11, 8:30 am to 11:05 am
Co-Chairs: Andrea Ablasser, École Polytechnique Fédérale de Lausanne; and Romeo Ricci, University of Strasbourg
8:30 am Introduction
8:35 am M180 Structural Basis for Influenza Virus Ns1 Protein Block of mRNA Nuclear Export. K. Zhang1, Y. Xie2, R. Muñoz-Moreno3, J. Wang1, L. Zhang4, M. Esparza1, B. M. A. Fontoura1, Y. Ren2; 1University of Texas Southwestern Medical Center, Dallas, TX, 2Vanderbilt University School of Medicine, Nashville, TN, 3Icahn School of Medicine at Mount Sinai, New York, NY, 4School of Life Sciences, Xiamen University, Xiamen, CHINA.
8:50 am M181 Moves and Countermoves: Viral-driven Evolution of Necroptotic Cell Death. S. N. Palmer, D. C. Hancks; University of Texas Southwestern Medical Center, Dallas, TX.
9:05 am M182 Stem Cell Immunoengineering for Universal Cardiac Therapy via CRISPR-Cas9. L. Randolph, X. Lian; Pennsylvania State University, University Park, PA.
9:20 am M183 Antibody-dependent Macrophage Phagocytosis Is Inhibited by Tall Bystander Proteins. A. Joffe, M. Bakalar, J. Hasnain, P. Geissler, D. Fletcher; UC Berkeley, Berkeley, CA.
9:35 am M184 Hepatokine Induction by Colchicine Prevents Systemic Inflammation via Activating PTPN6 Inhibitory Signaling. J. Weng1, P. Koch2, K. Shimada1, T. J. Mitchison1; 1Harvard Medical School, Boston, MA, 2Massachusetts General Hospital, Boston, MA.
9:50 am M185 Molecular Mechanisms of STING Pathway Activation and Inhibition. S. L. Ergun, D. Fernandez, L. Li; Biochemistry Dept, Stanford University, Stanford, CA.
10:05 am M186 INPP5E Is Required for Recruitment of the TCR/CD3 Complex to the Immune Synapse via the Ciliary Machinery. T. Chiu, F. Yang, J. Liao; Institute of Atomic and Molecular Sciences, Academia Sinica, Taipei, TAIWAN.
10:20 am M187 MISTR1 Is a Master Regulator of OXPHOS and Cell Death. M. Sorouri1,2, P. Jesudhasan1, D. C. Hancks1; 1UT Southwestern Medical Center, Dallas, TX, 2Baylor University, Waco, TX.
10:35 am M188 Ratio of Activating to Inhibitory Signaling Dominates Phagocytic Decision-Making. E. C. Suter, E. M. Schmid, A. M. Joffe, D. A. Fletcher; UC Berkeley, Berkeley, CA.
10:50 am M189 Organelle Membrane Contact Sites at the Origin of Nlrp3 Inflammasome Activation. R. Ricci, Z. Zhang, Z. Liu; IGBMC, Illkirch, FRANCE.
Co-Chairs: Darren Gilmour, University of Zurich; and Maddy Parsons, Kings College London
8:30 am Introduction
*8:35 am M190 E-cadherin Is an Invasion Suppressor, Survival Factor, and Metastasis Promoter in Multiple Models of Breast Cancer. V. Padmanaban1, I. Krol2, Y. Suhail1, B. Szczerba1, N. Aceto2, J. Bader1, A. Ewald1; 1Johns Hopkins University School of Medicine, Baltimore, MD, 2University of Basel, Basel, SWITZERLAND.
8:50 am M191 A Drosophila Model Visualizing Dissemination of Transformed Epithelial Cells Into the Circulation. Y. Kwon, J. Lee, A. Cabrera; University of Washington, Seattle, WA.
9:05 am M192 Tuning Cell Contractility and Vinculin Localization at Cell Junctions Is Required for Basal Epithelial Cell Extrusion. C. Villeneuve, E. Lagoutte, S. Mathieu, L. De Plater, J. Maitre, J. Manneville, P. Chavrier, C. Rosse; Institut Curie, Paris, FRANCE.
9:20 am M193 Epigenetic Heterogeneity within the Collective Invasion Pack Promotes Myo10-dependent Fibronectin Micropatterning by Leader Cells. E. R. Summerbell, J. K. Mouw, J. S. K. Bell, J. L. Arnst, T. O. Khatib, J. Konen, B. Dwivedi, S. Seby, J. Kowalski, P. M. Vertino, A. I. Marcus; Emory University, Atlanta, GA.
9:35 am M194 A Motile Sheath of Migratory Cells Supports Collective Migration of Epithelial Cells in Confinement during Migration of the Zebrafish Posterior Lateral Line Primordium. D. Dalle Nogare, A. Chitnis; NICHD, NIH, Bethesda, MD.
9:50 am M195 A Mechanistic Model of PLC/PKC Signaling Implicates Phosphatidic Acid as a Key Amplifier of Chemotactic Gradient Sensing. J. L. Nosbisch1, A. Rahman1, K. Mohan1, T. C. Elston2, J. E. Bear3,2, J. M. Haugh1; 1North Carolina State University, Raleigh, NC, 2University of North Carolina School of Medicine, Chapel Hill, NC, 3UNC Lineberger Comprehensive Cancer Center, Chapel Hill, NC.
10:05 am M196 Connectivity Analysis of GEF/GTPase Networks in Living Cells. D. J. Marston1, M. Vilela2, J. Ren1, G. Glekas1, M. L. Azoitei1, G. Danuser2, J. Sondek1, K. M. Hahn1; 1University of North Carolina-Chapel Hill, Chapel Hill, NC, 2University of Texas Southwestern Medical Center, Dallas, TX.
10:20 am M197 The LTB4-BLT1 Signaling Axis Coordinates Actomyosin Dynamics and Beta-2 Integrin Trafficking to Drive Intravascular Neutrophil Response to Infection. B. Subramanian1, N. Melis1, D. Chen1, W. Wang1, D. Gallardo1, R. Weigert1, C. Parent2; 1National Cancer Institute, NIH, Bethesda, MD, 2University of Michigan, Ann Arbor, MI.
10:35 am M198 Osmolarity-independent Cues Guide Rapid Cell Migration to Injury in Zebrafish Epidermis. A. S. Kennard1,2, J. A. Theriot2,3; 1Stanford University, Stanford, CA, 2University of Washington, Seattle, WA, 3Howard Hughes Medical Institute, Seattle, WA.
10:50 am M199 Migrating Neutrophils Execute Front Protrusion and Rear Retraction Programs with Certainty Until Doubt or Completion. A. Hadjitheodorou1, G. R. R. Bell2, F. Ellett3, D. Irimia3, R. Tibshirani4, S. R. Collins2, J. A. Theriot5; 1Department of Bioengineering, Stanford University, Stanford, CA, 2Department of Microbiology and Molecular Genetics, University of California Davis, Davis, CA, 3Department of Surgery, BioMEMS Resource Center Massachusetts General Hospital, Harvard Medical School, Boston, MA, 4Department of Statistics, Stanford University, Stanford, CA, 5Department of Biology, University of Washington, Seattle, WA.
*ASCB Merton Bernfield Awardee
Co-Chairs: Pedro Carvalho, University of Oxford; and Robin Klemm, University of Zürich
8:30 am Introduction
8:35 am M200 Single Molecule Characterization of Protein Dynamics at Endoplasmic Reticulum-Organelle Contact Sites. J. Nixon-Abell1, C. J. Obara1, F. Riccio2, J. Lippincott-Schwartz1, C. Blackstone3; 1Janelia Research Campus, Ashburn, VA, 2Kings College London, London, UNITED KINGDOM, 3National Institutes of Health (NINDS), Bethesda, MD.
8:50 am M201 Reticulon 3 Directly Regulates Late Endosome Trafficking on Microtubules. H. Wu, G. Voeltz; University of Colorado Boulder, Boulder, CO.
9:05 am M202 RNA Granules Hitchhike on Lysosomes for Long-distance Transport, Using Annexin A11 as a Molecular Tether. Y. Liao1, M. Fernandopulle2, G. Wang3, H. Choi1, L. Hao2, C. Drerup4, R. Patel2, S. Qamar3, J. Nixon-Abell3, Y. Shen5, W. Meadows3, M. Vendruscolo5, T. Knowles5,6, M. Nelson2, M. Czekalska3, G. Musteikyte3, M. A. Gachechiladze2, C. Stephens2, H. Pasolli1, L. Forrest2, P. S. George-Hyslop3,7, J. Lippincott-Schwartz1, M. E. Ward2; 1HHMI Janelia Research Campus, Ashburn, VA, 2NINDS, NIH, Bethesda, MD, 3Cambridge Institute for Medical Research, Department of Clinical Neurosciences, University of Cambridge, Cambridge, UNITED KINGDOM, 4NICHD, NIH, Bethesda, MD, 5Department of Chemistry, University of Cambridge, Cambridge, UNITED KINGDOM, 6Cavendish Laboratory, Department of Physics, University of Cambridge, Cambridge, UNITED KINGDOM, 7Department of Medicine (Division of Neurology), University of Toronto and University Health Network, Toronto, ON, CANADA.
9:20 am M203 The Significance of Sequestering H2A, H2Av and H2B on Lipid Droplets. R. A. Stephenson1, L. Chen1, J. M. Thomalla1, M. Beller2, M. A. Welte1; 1University of Rochester, Rochester, NY, 2Heinrich Heine University Düsseldorf, Düsseldorf, GERMANY.
9:35 am M204 Structure, Biogenesis, and Engineering of the Eukaryotic CO2-concentrating Organelle, the Pyrenoid. M. Meyer1, M. Jonikas1, S. He1, E. Freeman Rosenzweig2, A. Itakura2, N. Atkinson3, H. Chou4, T. Mettler-Altmann5, W. Patena1, T. Wunder6, J. Lau7, D. Matthies4, G. Yates7, V. Chen2, T. Olusola1, U. Goodenough8, M. Stitt9, B. Engel10, O. Mueller-Cajar6, Z. Yu4, A. Smith11, H. Griffiths12, A. McCormick3, L. Mackinder7; 1Princeton University, Princeton, NJ, 2Stanford University, Stanford, CA, 3University of Edinburgh, Edinburgh, UNITED KINGDOM, 4Howard Hughes Medical Institute, Ashburn, VA, 5University of Dusseldorf, Dusseldorf, GERMANY, 6Nanyang Technological University, Singapore, SINGAPORE, 7University of York, York, UNITED KINGDOM, 8Washington University in St. Louis, St. Louis, MO, 9Max Planck Institute of Molecular Plant Physiology, Golm, GERMANY, 10Max Planck Institute of Biochemistry, GERMANY, 11John Innes Centre, Norwich, UNITED KINGDOM, 12Cambridge University, Cambridge, UNITED KINGDOM.
9:50 am M205 Miga2 Links Mitochondria, the ER and Lipid Droplets and Promotes De Novo Lipogenesis in Adipocytes. C. A. Freyre1, R. W. Klemm1, P. C. Rauher1, C. S. Ejsing2,3; 1University of Zurich, Zurich, SWITZERLAND, 2University of Southern Denmark, Odense, DENMARK, 3Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, GERMANY.
10:05 am M206 Drosophila Adipocytes Maintain Spatially Distinct Lipid Droplet Sub-populations. M. Henne, R. Ugrankar, J. Bowerman; UT Southwestern Medical Center, Dallas, TX.
10:20 am M207 Two Forms of Opa1 Coordinate to Induce Mitochondrial Inner Membrane Fusion. Y. Ge1, S. Boopathy1, X. Shi2, A. Smith3, L. H. Chao1; 1The Massachusetts General Hospital, Boston, MA, 2Case Western Reserve University, Cleveland, OH, 3University of Akron, Akron, OH.
10:35 am M208 A Mitochondrial Translocon Subunit Promotes Assembly of the Cristae Organizing MICOS Complex in Proximity to ER Contact Sites. P. Tirrell, J. Friedman, R. Lohray, K. Nguyen; UT Southwestern, Dallas, TX.
10:50 am M209 Quality Control of Protein Complex Assembly by a Transmembrane Recognition Factor. P. Carvalho, N. Natarajan, O. Foresti; Sir William Dunn School of Pathology, University of Oxford, oxford, UNITED KINGDOM.
Co-Chairs: Stefano Di Talia, Duke University; and Silvia Santos, The Francis Crick Institute
8:30 am Introduction
8:35 am M210 Waves of ERK Activity Orchestrate Osteoblast Tissue Growth in Zebrafish Bone Regeneration. A. De Simone, M. Evanitsky, L. Hayden, B. Cox, J. Wang, A. Chao, K. Poss, S. Di Talia; Duke University, Durham, NC.
8:50 am M211 Mitogen-independent Cell Cycle Progression in B Lymphocytes. A. Singh1, M. Spitzer2, J. P. Joy1, G. P. Nolan2, R. Sen1; 1Gene Regulation Section, Laboratory of Molecular Biology and Immunology, National Institute on Aging, Baltimore, MD, 2Microbiology and Immunology - Baxter Labs, Center for Clinical Sciences Research, Stanford, CA.
9:05 am M212 Temporal Control of Cell Division: Switches, Feedback Control and Refractory Periods. A. Araujo1, S. Santos1, L. Gelens2, J. Ferrell3; 1The Francis Crick Institute, London, UNITED KINGDOM, 2University of Leuven, Leuven, BELGIUM, 3Stanford University, Palo Alto, CA.
9:20 am M213 Transient Hysteresis in Cdk4/6 Activity Underlies Passage of the Restriction Point in G1. T. Meyer, M. Chung, C. Liu; Stanford University School of Medicine, Stanford, CA.
9:35 am M214 Unconventional Growth and Division Patterns in Marine-derived Black Yeasts. L. M. Y. Mitchison-Field1,2, J. M. Vargas-Muñiz1, B. M. Stormo1, E. J. D. Vogt1, J. F. Pelletier3,2, C. M. Field3,2, A. S. Gladfelter1,2; 1UNC Chapel Hill, Chapel Hill, NC, 2Marine Biological Laboratory, Woods Hole, MA, 3Harvard Med School, Boston, MA.
9:50 am M215 Are Cancers Addicted to Aneuploidy? J. Sheltzer; Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
10:05 am M216 The Essential Plk1 Function in Centrosome Remodeling during Mitotic Entry Is Not PCM Expansion but γ-tubulin Complex Docking. M. Ota, Z. Zhao, S. Wang, J. Harrison, D. Wu, A. Desai, K. Oegema; Ludwig Institute for Cancer Research, University of California, San Diego, San Diego, CA.
10:20 am M217 PCH-2/TRIP13 Regulates Spindle Checkpoint Strength. L. Defachelles, A. Russo, C. Nelson, N. Bhalla; University of California, Santa Cruz, Santa Cruz, CA.
10:35 am M218 ATP Availability Limits Mitotic Duration and Impacts Cell Fate Determination. C. Ferrás1, J. Oliveira1, J. Monteiro1, A. Santos1, K. Bezstarosti2, J. Demmers2; 1IBMC/I3S, Porto, PORTUGAL, 2Erasmus University Medical Center, Rotterdam, NETHERLANDS.
10:50 am M219 Loss of Epithelial Cell Size Regulation in Lung Cancer. C. Sandlin, S. Gu, C. Deshpande, M. Feldman, M. Good; University of Pennsylvania, Philadelphia, PA.
Co-Chairs: Laura Anne Lowery, Boston College; and Kassandra Ori-McKenney, University of California, Davis
8:30 am Introduction
8:35 am M220 Reconstituting Cytoskeletal Assembly from Budding Yeast Extracts Reveals Basic Biophysical Properties of Septin Filament Polymerization. B. Woods, K. Cannon, A. Gladfelter; University of North Carolina, Chapel Hill, NC.
8:50 am M221 Plastin Promotes Fast Actin Filament Bundling Along with Formin-mediated Polymerization to Generate Rapid Filament Alignment during Contractile Ring Assembly. R. S. Kadzik1, Y. Li2,3, D. Kovar4,3, S. Wignall1, E. Munro3,2; 1Department of Molecular Biosciences, Northwestern University, Evanston, IL, 2Committee on Development, Regeneration, and Stem Cell Biology, University of Chicago, Chicago, IL, 3Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, IL, 4Department of Biochemistry and Molecular Biology, University of Chicago, Evanston, IL.
9:05 am M222 Interplay among Human EB1, APC, and Dia1 in Coordinating Microtubule and Actin Dynamics. M. A. Juanes, R. Jaiswal, C. Fees, B. L. Goode; Brandeis University, Waltham, MA.
9:20 am M223 Clasp Mediates Microtubule Repair by Promoting Tubulin Incorporation into Damaged Lattices. A. Aher1, D. Rai1, L. Schaedel2, J. Gaillard2, K. John2, L. Blanchoin2,3, M. Thery3,2, A. Akhmanova1; 1Cell Biology, Department of Biology, Faculty of Science, Utrecht University, Utrecht, NETHERLANDS, 2Univ. Grenoble-Alpes, CEA, CNRS, INRA, Biosciences & Biotechnology Institute of Grenoble, Laboratoire de Phyiologie Cellulaire & Végétale, CytoMorpho Lab, Grenoble, FRANCE, 3Univ. Paris Diderot, INSERM, CEA, Hôpital Saint Louis, Institut Universitaire d’Hematologie, UMRS 1160, CytoMorpho Lab, Paris, France, Paris, FRANCE.
9:35 am M224 A Combinatorial MAP Code Dictates Polarized Microtubule Transport. B. Monroy1, K. M. Ori-McKenney1, T. Tan1, J. Oclaman1, D. W. Nowakowski2, R. J. McKenney1; 1University of California, Davis, Davis, CA, 2N Molecular Systems Inc., Palo Alto, CA.
9:50 am M225 ARL8 Dependent Autoinhibition of SKIP Regulates Its Association with Kinesin-1. T. Keren-Kaplan, J. S. Bonifacino; NIH/NICHD, Bethesda, MD.
*10:05 am M226 Regulation of Mbp Local Translation Is Crucial for Adult Myelin Maintenance. M. Fu, A. Valenzuela, B. Barres; Stanford University, Stanford, CA.
10:20 am M227 A Pair of E3 Ubiquitin Ligases Compete to Regulate Filopodial Dynamics and Axon Guidance. N. P. Boyer, S. Gupton, L. McCormick, S. Menon, F. Urbina; University of North Carolina-Chapel Hill, Chapel Hill, NC.
10:35 am M228 Phosphorylation of the +TIP, TACC3, Modulates Its Interaction with Microtubules and Affects Axon Outgrowth and Guidance. B. Erdogan1, L. Lowery1, G. Cammarata1, R. St. Clair2, B. Ballif2; 1Boston College, Chestnut Hill, MA, 2University of Vermont, Burlington, VT.
10:50 am M229 Nestin Is a New Binding Partner of DCX and Regulates Its Cdk5-mediated Phosphorylation and DCX-dependent Growth Cone Morphology. C. J. Bott1, B. Winckler1, J. M. Keil2, L. McMahon1, C. Yap1, K. Y. Kwan2; 1University of Virginia, University of Virginia, VA, 2University of Michigan, University of Michigan, MI.
*ASCB Porter Prize Awardee for Research Excellence