Microsymposia
Sunday, December 8, 11:00 am to 12:00 noon
Room 151B
Supported by The International Journal of Biochemistry & Cell Biology - Elsevier
Moderator: Natalya Ortolano, Vanderbilt University
11:00 am MS1 The Role of Optineurin in Neuronal Mitophagy . C. S. Evans, E. L. F. Holzbaur; University of Pennsylvania, Philadelphia, PA.
11:10 am MS2 Structural Mechanism of Folliculin-mediated Regulation of the Rag Gtpase Activation Cycle. R. Lawrence, S. Fromm, Y. Fu, A. Yokom, D. Kim, A. Thelen, L. Young, J. Hurley, R. Zoncu; UC Berkeley, Berkeley, CA.
11:20 am MS3 Impaired Lysosome Transport to Distal Axons Contributes to Autophagic Stress in the Neurodegenerative Lysosomal Storage Disorder Niemann-Pick Type C. J. C. Roney1,2, T. Farfel-Becker1, X. Cheng1, F. M. Platt2, Z. Sheng1; 1Synaptic Function Section, National Institute of Neurological Disorders and Stroke, NIH, Bethesda, MD, 2Department of Pharmacology, University of Oxford, Oxford, UNITED KINGDOM.
11:30 am MS4 Mechanism of a Memory-enhancing Inhibitor of the Integrated Stress Response. A. A. Anand1, L. R. Kenner2, J. C. Tsai1, L. Miller-Vedam2, H. C. Nguyen2, P. Jaishankar2, A. G. Myasnikov2, C. J. Klose1, L. A. McGeever1, A. Frost2, P. Walter1; 1UCSF, Howard Hughes Medical Institute, San Francisco, CA, 2UCSF, San Francisco, CA.
11:40 am MS5 A Close-up View of Mitophagy Using Mt-Keima and Fluorescence Lifetime Microscopy. D. Malide1, N. Sun2,1, T. Finkel3,1; 1National Institutes of Health, Bethesda, MD, 2Ohio State University Wexner Medical Center, Columbus, OH, 3Aging Institute, University of Pittsburgh Medical Center, Pittsburgh, PA.
11:50 am MS6 P27 Regulates the Autophagy-lysosomal Pathway via the Control of Ragulator and MTOR Activity in Amino Acid Deprived Cells. A. Besson, A. Nowosad; CNRS, Toulouse, FRANCE.
Room 201
Moderators: Alyssa Lesko, University of Virginia School of Medicine; and Valerie Tutwiler, University of Pennsylvania
11:00 am MS7 A Role for the Apical PAR Complex in Reorganizing Microtubules in Dividing Intestinal Cells. M. Sallee, J. Feldman; Stanford University, Stanford, CA.
11:10 am MS8 Integrating Neutrophil Fronts and Backs with the Mtorc2 Mechanotransduction Pathway. S. Saha, O. D. Weiner; Cardiovascular Research Institute and Department of Biochemistry and Biophysics, UCSF, San Francisco, CA.
11:20 am MS9 A Novel Labeling Strategy Reveals That Neuronal Myosin V-labeled Vesicles Are Polarized to Dendrites. M. Frank, M. Bentley; Department of Biological Sciences and the Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY.
11:30 am MS10 Rab19 Mediates Formation of a Trafficking Super Complex That Regulates Primary Ciliogenesis. C. E. Jewett, R. Prekeris; University of Colorado Anschutz Medical Campus, Aurora, CO.
11:40 am MS11 A Centriole-less Pericentriolar Material Serves As the Base. S. Eskinazi, J. Magescas, J. L. Feldman; Stanford University, Stanford, CA.
11:50 am MS12 Cilia Development in Zebrafish Organ of Asymmetry. J. Manikas, L. Rathbun, J. Freshour, H. Hehnly; Syracuse University, Syracuse, NY.
of C. elegans Sensory Cilia. S. Eskinazi, J. Magescas, J. L. Feldman; Stanford University, Stanford, CA.
Room 146C
Moderators: Amanda S. Meyer, University of Southern California; and Margherita Perillo, Brown University
11:00 am MS13 The Chlamydia Trachomatis Effector TepP Reprograms the Function of the F-actin Regulator Eps8 to Mediate the Transient Disassembly of Epithelial Cell-cell Junctions to Regulate Innate Immune Responses. L. Dolat, R. Valdivia; Duke University Medical Center, Durham, NC.
11:10 am MS14 Disruption of TFAM in T Lymphocytes Leads to Impaired Mtdna Copy Number Regulation, Altered CD8+ T Cell Effector Function and Metabolism. S. Kapnick, R. Genner, P. McGuire; National Institutes of Health, Bethesda, MD.
11:20 am MS15 Axon-glial Interactions: Oligodendrocyte-mediated Enhancement of Axonal Energy Metabolism via Post-translational Acetylation of Neuronal Mitochondria. K. A. Chamberlain, F. LiCausi, Z. H. Sheng; National Institute of Neurological Disorders and Stroke, NIH, Bethesda, MD.
11:30 am MS16 Analysis of Early Cellular and Sub-cellular Changes During Tumor Initiation and Progression in Live Animals. W. Wang, 20892, K. Rechache, R. Weigert; National Institutes of Health, Bethesda, MD.
11:40 am MS17 CARD19 Interacts with MICOS Complex Proteins and Protects Against Mitochondrial Dysfunction. K. Rios1,2, C. Beauregard1,2, M. Zhou3, T. P. Conrads3, B. Schaefer1; 1USUHS, Bethesda, MD, 2HJF, Rockville, MD, 3Inova Schar Cancer Institute, Annandale, VA.
11:50 am MS18 Septin Recruitment of Contractile Acto-myosin Cages to Vesicle Membranes Drives Regulated Exocytosis in Exocrine Glands of Live Mice. S. Ebrahim, M. Weiss, D. Chen, R. Weigert, NCI/NIH, Bethesda, MD.
Room 145A
Supported by The Allen Institute
Moderator: Ashley Lakoduk, University of Texas Southwestern Med Center, Dallas
11:00 am MS19 Designer Membraneless Organelles Equip Eukaryotic Cells with a Second Genetic Code to Enable Orthogonal Translation of Selected Messenger RNAs. C. D. Reinkemeier, G. Estrada Girona, E. A. Lemke; EMBL, Heidelberg, GERMANY.
11:10 am MS20 Viral Assembly Site Phase-separation Mediated By Transbilayer-coupling and Membrane Curvature Drives Selective Protein Incorporation into HIV Membranes. P. Sengupta1, A. Seo1, A. Pasolli1, M. Johnson2, J. Lippincott-Schwartz3; 1HHMI, Ashburn, VA, 2University of Missouri, Columbia, MO, 3HHMI, Janelia Research Campus, Ashburn, VA.
11:20 am MS21 Domain Characterization of Pericentrin Uncovers the Role of Its Intrinsically Disordered Regions in Mediating Phase Separation and Microtubule Nucleation in Human Cells. X. Jiang, K. Mahe, D. Ho, J. Mia, S. Yamada, L. Jao; University of California, Davis, Davis, CA.
11:30 am MS22 Presynapse Active Zones Assemble through Phase Separation of Scaffolding Molecules. N. McDonald, K. Shen; Stanford University, Stanford, CA.
11:40 am MS23 Sequence Determinants of Laf-1 Phase Separation in P Granule Assembly. B. Schuster1, G. Dignon2, C. Jahnke3, D. Hammer3, M. Good3, J. Mittal2; 1Rutgers University, New Brunswick, NJ, 2Lehigh University, Bethlehem, PA, 3University of Pennsylvania, Philadelphia, PA.
11:50 am MS24 P-bodies Mediate the Post-transcriptional Regulation of Draxin During Neural Crest EMT. E. J. Hutchins, M. L. Piacentino, R. Galton, G. da Silva Pescador, M. E. Bronner; California Institute of Technology, Pasadena, CA.
Room 206
Moderators: Caitlyn Blake-Hedges, Florida State University; and Scott Wilkinson, National Institutes of Health
11:00 am MS25 Systems Biology Identifies Gleevec As a Specific Inhibitor of CLIP-170S, a Novel +Tip Isoform, Which Causes Taxane Resistance in Cancer Cells and Patients by Obstructing the Microtubule Pore. P. V. Thakkar1, K. Kita1, G. Galletti1, N. S. Madhukar1, E. V. Navarro1, K. Cleveland1, I. Barasoain2, H. V. Goodson3, D. L. Sackett4, J. F. Diaz2, O. Elemento1, M. A. Shah1, P. Giannakakou1; 1Weill Med College/Cornell University, New York, NY, 2Centro de Investigaciones Biológicas, Madrid, SPAIN, 3University of Notre Dame, Notre Dame, IN, 4Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD), NIH, Bethesda, MD.
11:10 am MS26 Kinesin-based Transport Is Controlled by Cholesterol in the Cargo Membrane. Q. Li1, J. Wilson1, K. Tseng2, W. Qiu2, S. King3, M. Vershinin4, J. Xu1; 1University of California, Merced, Merced, CA, 2Oregon State University, Corvallis, OR, 3University of Central Florida, Orlando, FL, 4University of Utah, Salt lake city, UT.
11:20 am MS27 Structural Insights Into the Chemical Inhibition of Dynein. C. C. Santarossa1, K. J. Mickolajczyk1, L. Urnavicious1, N. Coudray2, J. B. Steinman1, D. Ekiert2, G. Bhabha2, T. M. Kapoor1; 1The Rockefeller University, New York, NY, 2New York University, New York, NY.
11:30 am MS28 A Pushing Mechanism for Microtubule Aster Positioning in a Large Cell Type. J. Meaders, D. R. Burgess; Boston College, Chestnut Hill, MA.
11:40 am MS29 Vasohibins-Mediated Microtubule Detyrosination Regulates Mitotic Spindle Morphology and Orientation through Kinesin13/MCAK. G. Rajendraprasad1, S. Eibes1, S. Liao2, C. Xu2, M. Barisic1; 1Danish Cancer Society, Copenhagen, DENMARK, 2Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, Hefei, CHINA.
11:50 am MS30 RETROGRADE Transport Is Required for Mitochondrial Health and Function in Neurons. A. Mandal, K. Pinter, C. M. Drerup; NIH/NICHD, Bethesda, MD.
Room 147A
Moderator: Joseph Varberg, Stowers Institute for Medical Research
11:00 am MS31 The DNA Repair Protein Nopo Has a Mitotic Function That Suppresses Neuronal Stress Response to Prevent Microcephaly. R. S. O'Neill, C. J. Fagerstrom, N. M. Rusan; National Heart, Lung, and Blood Institute, NIH, Bethesda, MD.
11:10 am MS32 The Mechanical Integrity of the Mammalian K-fiber and Its Molecular Origin. M. A. Begley1, E. M. Davis1, R. Ohi2, M. W. Elting1; 1North Carolina State University, Raleigh, NC, 2University of Michigan, Ann Arbor, MI.
11:20 am MS33 Anti-tropomyosin Drugs Prevent the Rescue of Vincristine-induced Mitotic Spindle Defects. P. W. Gunning1, Y. Wang1, J. H. Stear1, A. Swain1, X. Xu1, N. Bryce1, I. B. Alieva2, V. B. Dugina2, T. Cripe3, J. Stehn1, E. C. Hardeman1; 1University New South Wales, Sydney, AUSTRALIA, 2Moscow State University, Moscow, RUSSIAN FEDERATION, 3Nationwide Children's Hospital, Columbus, OH.
11:30 am MS34 A Minimal Spindle-Midzone Protein Module Differentially Regulates Single Microtubules and Crosslinked Microtubule Arrays. N. Mani, S. Jiang, R. Subramanian; Harvard Medical School / Massachusetts General Hospital, Boston, MA.
11:40 am MS35 Branching Microtubule Nucleation Is the Main Source of Microtubules Generated at Chromosomes in Meiotic Xenopus Egg Extracts. S. U. Setru, J. W. Shaevitz, S. Petry; Princeton University, Princeton, NJ.
11:50 am MS36 Identification of Novel Synaptonemal Complex Components in C. Elegans. M. E. Hurlock1, I. Čavka2, J. M. Haversat1, M. Wooten1, Z. Nizami3, R. Turniansky1, P. Hoess2, J. Ries2, J. G. Gall3, S. Köhler2, Y. Kim1; 1Johns Hopkins University, Baltimore, MD, 2The European Molecular Biology Laboratory, Heidelberg, GERMANY, 3Department of Embryology, Carnegie Institution for Science, Baltimore, MD.
Monday, December 9, 11:00 am to 12:00 noon
Room 146C
Supported by The Allen Institute
Moderators: Vladimir Botchkarev, Dana-Farber Cancer Institute/Harvard Medical School; and Chenshu Liu, University of California, Berkeley/HHMI
11:00 am MS37 Linc-ing the Nucleus and the Cytoskeleton. P. M. Davidson1, A. Battistella1, T. Déjardin1, T. Betz2, J. Plastino1, B. Cadot3, N. Borghi4, C. Sykes1; 1Institut Curie, Paris, FRANCE, 2Institute of Cell Biology, ZMBE, Munster, GERMANY, 3Center for Research in Myology, Paris, FRANCE, 4Institut Jacques Monod, Paris, FRANCE.
11:10 am MS38 Increased Lamin B1 Levels Promote Cancer Cell Migration by Altering Perinuclear Actin Organization. A. Fracchia, G. Gerlitz; Ariel University, Ariel, ISRAEL.
11:20 am MS39 Climp-63 Regulates Nuclear Movement during Polarization of Migrating Cells. C. S. Janota, J. Costa, A. Pezzarossa, E. R. Gomes; Instituto de Medicina Molecular, Lisbon, PORTUGAL.
11:30 am MS40 Macronuclear Shape Change In the Giant Ciliate, Stentor Coeruleus. R. M. McGillivary, P. Sood, W. F. Marshall; University of California, San Francisco, San Francisco, CA.
11:40 am MS41 Nuclear Pore Complex Scaffold Experiences Conformational Changes In Vivo In Response to Transport State. J. Pulupa, H. Prior, S. M. Simon; Rockefeller University, New York, NY.
11:50 am MS42 Mechanosensitive Phospho-dependent Recruitment of Baf to the Nuclear Membrane Inhibits Nuclear Accumulation of E2f1 and Yorkie. T. Volk,, U. CP, A. Reuveny, D. Grunbaum; Weizmann Institute of Science, Rehovot, ISRAEL.
Room 147A
Moderators: Alyssa Lesko; University of Virginia School of Medicine; and Sumana Sundaramurthy; SUNY Upstate Medical University
11:00 am MS43 Single Cell Dynamics Driving Vertebrate Heart Valve Interstitial Layer Morphogenesis. F. Gunawan, A. Gentile, S. Gauvrit, D. Stainier, A. Bensimon-Brito; Max Planck Institute for Heart and Lung Research, Bad Nauheim, GERMANY.
11:10 am MS44 Anisotropic Cues Promote Symmetry Breaking To Initiate Migration Of Adherent Cells. D. Li, Y. Wang;
Carnegie Mellon University, Pittsburgh, PA.
11:20 am MS45 Mechanically Driven Cellular Competition Promotes the Collective Extrusion of Bacteria-infected Epithelial Cells. E. E. Bastounis1, P. Rhadhakrishnan1, P. Engström2, F. Alcalde3, M. J. Gómez Benito3, J. M. García Aznar3, M. D. Welch2, J. A. Theriot1; 1University of Washington, Seattle, WA, 2University of California, Berkeley, Berkeley, CA, 3University of Zaragoza, Zaragoza, SPAIN.
11:30 am MS46 Tissue Stiffness Modulates Extracellular Vesicle Function In Metastatic Breast Cancer. A. Sneider1, Y. Liu2, C. Marar1, N. Faqih1, G. Ciotti2, J. Kim1, M. Igboko1, S. Ibrahim3, S. Krishnan4, A. Locke5, B. Starich1, G. Russo1, M. Karl1, P. Nair1, R. Vij1, E. Gomez-de-Mariscal6, D. Lewis1, A. Munoz-Barrutia6, L. Gu1, T. Eisinger2, D. Wirtz1; 1Johns Hopkins University, Baltimore, MD, 2University of Pennsylvania, Philadelphia, PA, 3Pacific Lutheran University, Tacoma, WA, 4Harker School, San Jose, CA, 5Lamar University, Beaumont, TX, 6Universidad Carlos III de Madrid, Madrid, SPAIN.
11:40 am MS47 Cellular Herding: Programming Collective Cell Migration through Electrotaxis. D. Cohen; Princeton University, Princeton, NJ.
11:50 am MS48 Single-Cell RNA-Sequencing of Lung Cancer Leader and Follower Cells Reveals Distinct Mutational Profiles and Cancer Stem Cell-Like Gene Expression Patterns. B. Pedro1, J. M. Konen2, M. Rupji3, B. Dwivedi3, J. Kowalski4, P. M. Vertino5, A. I. Marcus1; 1Emory University, Atlanta, GA, 2University of Texas MD Anderson Cancer Center, Houston, TX, 3Winship Cancer Institute, Atlanta, GA, 4The University of Texas at Austin, Austin, TX, 5University of Rochester Medical Center, Rochester, NY.
Room 206
Moderators: Tim Fessenden, Massachusetts Institute of Technology; and Emily Summerbell, Emory University
11:00 am MS49 Leveraging Population-Level Intercellular Variation of 2D Cell Shape for Mechanistic Insight into Cell Migration. R. D. Labitigan1,2, A. L. Sanborn1, C. V. Hao1, J. A. Theriot2; 1Stanford University, Stanford, CA, 2University of Washington, Seattle, WA.
11:10 am MS50 Osteoporosis Mutations Lead to Impaired Calcium Regulation of Actin Bundling by Plastin-3. E. Kudryashova1, C. Schwebach1, W. Zheng2, M. Orchard1, L. Runyan1, H. Smith1, E. Egelman2, D. Kudryashov1; 1Ohio State University, Columbus, OH, 2University of Virginia, Charlottesville, VA.
11:20 am MS51 Role of the Actin Severing Factors, ADF/cofilin and Gelsolin, in Regulating the Fast Amoeboid or Leader Bleb-based Migration of Cancer Cells. M. F. Ullo, J. S. Logue; Albany Medical College, Albany, NY.
11:30 am MS52 Cell Matrix Invasion Requires Non-Muscle Myosin 2A/B Polarity and Nuclear-force Coupling Generated from Adhesion Sites. L. E. Young1, T. Newman1, T. Waring1, E. Madondald1, P. Caswell2, L. Machesky3, M. Morgan1, T. Zech1; 1University of Liverpool, Liverpool, UNITED KINGDOM, 2University of Manchester, Manchester, UNITED KINGDOM, 3Beatson Institute, Glasgow, UNITED KINGDOM.
11:40 am MS53 Uncovering Microtubule-driven Mechanisms of Melanoma Invasion. R. J. Ju, Y. Chhabra, N. K. Haass, S. J. Stehbens; University of Queensland, Brisbane, AUSTRALIA.
11:50 am MS54 Microtubule Acetylation Promotes Rigidity Sensing and Mechanosensitive Migration of Astrocytes. S. Seetharaman1, B. Vianay2, V. Roca1, S. Etienne-Manneville1; 1Institut Pasteur, Paris, FRANCE, 2Paris University, INSERM, CEA, Hôpital Saint Louis, Institut Universitaire d’Hematologie, Paris, FRANCE.
Room 151B
Moderators: Ashley Rowland, University of Colorado, Boulder; and Sara Wong, University of Michigan.
11:00 am MS55 Phase-partitioning of Vacuole Membrane Directs Inter-organellar Sterol Trafficking to Coordinate Mitochondrial Development and Energy Metabolism with Macro-autophagy. A. Y. Seo1, F. Sarkleti1, I. Budin2, J. Lippincott-Schwartz1; 1HHMI-Janelia Research Campus, Ashburn, VA, 2University of California San Diego, San Diego, CA.
11:10 am MS56 Mitochondrial Fragmentation as a Mechanism for Localized Signaling . A. Horn, S. Raavicharla, S. Shah, J. K. Jaiswal; Children's National Health System, Washington, DC.
11:20 am MS57 Spatial Organization of Mitochondrial Inner Membrane Subdomains. K. Subramanian1, S. Barger2, A. Guna3, J. Nunnari1; 1University of California, Davis, Davis, CA, 2SUNY Upstate Medical University, Syracuse, NY, 3MRC Laboratory of Molecular Biology, Cambridge, UNITED KINGDOM.
11:30 am MS58 ER Membrane Contact Sites Are a Platform for Regulating Mitochondrial Dynamics and Bioenergetics. R. G. Abrisch1, S. C. Gumbin2,3, G. K. Voeltz2,3; 1Biochemistry, University of Colorado Boulder, Boulder, CO, 2Molecular, Cellular, and Developmental Biology, University of Colorado Boulder, Boulder, CO, 3Howard Hughes Medical Institute, Boulder, CO.
11:40 am MS59 Synergistic Role of Two Parkinson's Disease Linked Phosphoinositide Phosphatases in Presynaptic Endocytic Trafficking. M. Cao1,2, D. Park1, Y. Wu1, P. De Camilli1; 1Departments of Neuroscience and Cell Biology, Howard Hughes Medical Institute, Program in Cellular Neuroscience, Neurodegeneration and Repair, Kavli Institute for Neuroscience, School of Medicine, Yale University, New Haven, CT, 2Present address: Program in Neuroscience and Behavioural Disorders, Duke-NUS Medical School, Singapore, SINGAPORE.
11:50 am MS60 Rafting with Rush: Membrane Rafts Mediate Protein Trafficking through the Biosynthetic Pathway. I. Castello Serrano, B. Diaz-Rohrer, F. A. Heberle, R. Ippolito, K. R. Levental, I. Levental; UTHealth | the University of Texas Health Science Center at Houston, Houston, TX.
Room 201
Moderators: Caitlyn Blake-Hedges, Florida State University; and Amanda Haage, University of North Dakota
11:00 am MS61 The Effect of Tubulin Arginylation on Cellular Microtubules. B. MacTaggart, A. Kashina; University of Pennsylvania, Philadelphia, PA.
11:10 am MS62 Unexpected Selective Coordination between Axonal Lysosome Transport and Microtubule Dynamics with Neurodegenerative Disease Implications. N. Mohd Rafiq, L. Lyons, S. Gowrishankar, P. De Camilli, S. Ferguson; Yale University, New Haven, CT.
11:20 am MS63 Growing Tip-localized Microtubule Organizing Center Determines Microtubule Orientation in Dendrites. M. Kokes1,2, X. Liang1,2, M. A. Pickett1, M. D. Sallee1, J. L. Feldman1, K. Shen1,2; 1Stanford University, Stanford, CA, 2Howard Hughes Medical Institute, Stanford, CA.
11:30 am MS64 Hierarchical Regulation of Spindle Scaling during Development. E. M. Rieckhoff1,2,3, F. Berndt1,2,3, S. Golfier1,2,3, F. Decker1,2,3, J. Brugués1,2,3; 1Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, GERMANY, 2Max Planck Institute for the Physics of Complex Systems, Dresden, GERMANY, 3Center for Systems Biology Dresden, Dresden, GERMANY.
11:40 am MS65 Effect of Cytoplasm Concentration on Cytoskeleton Dynamics. A. T. Molines1, J. Lemière1, A. Serra-Marques1, G. Goshima2, F. Chang1; 1UCSF, San Francisco, CA, 2Nagoya University, Nagoya, JAPAN.
11:50 am MS66 A Gelation Transition Enables Long-ranged Active Flows That Organize Xenopus Egg Extract Spindles. D. Oriola, B. Dalton, F. Decker, F. Julicher, J. Brugues; Max Planck Institute, Dresden, GERMANY.
Room 145A
Moderator: Sam Dundon, Yale University
11:00 am MS67 The Ubiquitin Ligase Trim37 Controls Acentrosomal Spindle Formation and Cancer-specific Vulnerability to Plk4 Inhibition. F. Meitinger, M. Ohta, K. Lee, S. Watanabe, R. L. Davis, J. V. Anzola, R. Kabeche, D. Jenkins, A. K. Shiau, A. Desai, K. Oegema; Ludwig Institute for Cancer Research, San Diego, CA.
11:10 am MS68 Exploiting Trim37-driven Centrosome Dysfunction to Eliminate 17q23-amplified Breast Cancer Cells. Z. Yeow1, B. Lambrus2, M. Durin1, D. Moralli1, C. Green1, R. Chapman1, A. Holland2; 1Wellcome Centre for Human Genetics, University of Oxford, Oxford, UNITED KINGDOM, 2Johns Hopkins University School of Medicine, Baltimore, MD.
11:20 am MS69 Cyclin B1 Scaffolds MAD1 at the Corona to Activate the Spindle Assembly Checkpoint. L. A. Allan1, M. Reis1, Y. Liu2, P. Huis in ’t Veld2, G. J. Kops3, A. Musacchio2, A. T. Saurin1; 1University of Dundee, Dundee, UNITED KINGDOM, 2Max Planck Institute of Molecular Physiology, Dortmund, GERMANY, 3Hubrecht Institute, Utrecht, NETHERLANDS.
11:30 am MS70 Intramolecular Regulation of Anillin during Cytokinesis. D. Beaudet, N. Pham, N. Skaik, A. Piekny; Concordia University, Montreal, QC, CANADA.
11:40 am MS71 Actin-independent, Microtubule-dependent Cytokinesis in Chlamydomonas. M. Onishi1, J. E. Little2, T. Frej3, J. R. Pringle1; 1Stanford University, Stanford, CA, 2Gunn High School, Palo Alto, CA, 3Carnegie Institution for Science, Stanford, CA.
11:50 am MS72 Phase Separation by Membrane Protein Lem2 Controls ESCRT-mediated Nuclear Envelope Reformation. A. Von Appen1, D. LaJoie2, I. Johnson1, M. Trnka3, A. Burlingame3, K. Ullman2, A. Frost1; 1UCSF - Department of Biochemistry and Biophysics, San Francisco, CA, 2Huntsman Cancer Institute, University of Utah, Salt Lake City, UT, 3UCSF - Department of Pharmaceutical Chemistry, San Francisco, CA.
Tuesday, December 10, 11:00 am to 12:00 noon
Room 201
Moderators: Matt Akamatsu, University of California, Berkeley; and Kristen Skruber, University of Florida
11:00 am MS73 Intravital Subcellular and Single Molecule Imaging Reveal Multiple Actin Filament Populations Collaborate in the Remodelling of the Secretory Granule Membrane. M. Heydecker1, A. Masedunskas1, S. Ebrahim2, M. Appaduray1, A. Shitara2, N. Bryce1, R. Weigert2, P. Gunning1, E. Hardeman1; 1School of Medical Sciences, University of New South Wales, Sydney, AUSTRALIA, 2Laboratory of Cellular and Molecular Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD.
11:10 am MS74 The Role of APC-mediated Actin Assembly in Microtubule Capture and Focal Adhesion Turnover. M. A. Juanes1, D. Isnardon2, A. Badache2, S. Brasselet3, M. Mavrakis3, B. L. Goode1; 1Brandeis University, Waltham, MA, 2Centre de Recherche en Cancérologie de Marseille, Inserm, Institut Paoli-Calmettes, Aix Marseille Université, Marseille, FRANCE, 3Aix Marseille Université, CNRS, Centrale Marseille, Institut Fresnel, Marseille, FRANCE.
11:20 am MS75 SPIN90 Links Arp2/3 Complex Nucleation to Formin Elongation to Control Actin Network Organization. L. Cao1, G. Charras2, A. Jegou1, G. Romet-Lemonne1; 1CNRS - Institut Jacques Monod, Paris, FRANCE, 2University College London, London, UNITED KINGDOM.
11:30 am MS76 Vimentin Intermediate Filaments and F-actin Form Interpenetrating Networks in the Cell Cortex. H. Wu1, Y. Shen1, S. Sivagurunathan2, M. Weber3, O. Medalia3, R. Goldman2, D. Weitz1; 1John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, MA, 2Department of Cell and Developmental Biology, Northwestern University Feinberg School of Medicine, Chicago, IL, 3Department of Biochemistry, University of Zurich, Zurich, SWITZERLAND.
11:40 am MS77 Identification of a Novel B Cell Actomyosin Network That Promotes Antigen Contraction during Immune Synapse Formation. J. C. Wang, X. Wu, J. A. Hammer; National Institutes of Health, Bethesda, MD.
11:50 am MS78 ⍺T-catenin Intramolecular Interactions Regulate Vinculin Binding. J. A. Heier1, S. Pokutta2, W. I. Weis2, A. V. Kwiatkowski1; 1University of Pittsburgh School of Medicine, Pittsburgh, PA, 2Stanford University School of Medicine, Stanford, CA.
Room 206
Moderators: Emily Summerbell, Emory University; and Sumana Sundaramurthy, SUNY Upstate Medical University
11:00 am MS79 Single-molecule Imaging Reveals Distinct Subcomplexes of the Bacillus Subtilis Division Machinery. M. J. Holmes, E. C. Garner; Harvard University, Cambridge, MA.
11:10 am MS80 The Architecture and Dynamics of Podosomes in Macrophage Frustrated Phagocytosis. S. Hu1, T. Watanabe1, A. T. Nogueira1, J. Aaron2, A. Taylor2, T. Chew2, K. Hahn1; 1University of North Carolina At Chapel Hill, Chapel Hill, NC, 2HHMI Janelia Research Campus, Ashburn, VA.
11:20 am MS81 The Cellular Mechanism of Structural Color Change in the Zebrafish. D. Gur1,2, X. Wu3, J. Hammer4, J. Lippincott Schwartz2; 1NIH/NICHD, Bethesda, MD, 2Janelia Research Campus, Ashburn, VA, 3NIH/NHLBI, Bethesda, MD, 4NIH\NHLBI, Bethesda, MD.
11:30 am MS82 Development of An Optogenetic Tool to Reversibly Control Microtubule Acetylation. A. Deb Roy, E. Gross, G. Pillai, A. Kim, T. Inoue; Johns Hopkins University, Baltimore, MD.
11:40 am MS83 Systematic Characterization of a Large Number of Microtubule-Associated Proteins Using Purification-free Tirf-reconstitution Assays. A. S. Jijumon1,2,3, S. Bodkuntla1,3, M. Genovo1,2, M. Bangera4, F. Maksut1,2, M. M. Magiera1,2, C. Janke1,2,3; 1Institut Curie, Paris, FRANCE, 2University of Paris Saclay, Orsay, FRANCE, 3PSL University, Paris, FRANCE, 4Instem, Bangalore, INDIA 11:50 am MS84 Three-color Tracking of Dynein Stepping Along Microtubules. S. Niekamp, N. Stuurman, R. D. Vale; University of California - San Francisco, San Francisco, CA.
Room 145A
Moderator: Vladimir Botchkarev, Dana-Farber Cancer Institute/Harvard Medical School
11:00 am MS85 Generation of Regulatory Stable Intronic Sequence RNAs from Conserved Genetic Loci . S. Chan1, R. B. Ismail1, J. Heng2, S. Lim3, J. Ho4, J. Pek1; 1Temasek Lifesciences Laboratory, Singapore, SINGAPORE, 2Raffles Institution, Singapore, SINGAPORE, 3Temasek Polytechnic, Singapore, SINGAPORE, 4Ngee Ann Polytechnic, Singapore, SINGAPORE.
11:10 am MS86 SETD2 Loss Drives Genomic Instability by Increasing CENP-A Levels and Generation of Dicentric Chromosomes. F. M. Mason1, E. S. Kounlavong1, I. Park2, C. L. Walker2, W. K. Rathmell1; 1Vanderbilt University Medical Center, Nashville, TN, 2Baylor College of Medicine, Houston, TX.
11:20 am MS87 Compaction without Condensin? Using Oligopaints to Investigate Chromosome Territories in the Moth, Bombyx Mori. L. F. Rosin1, J. Gil, Jr.2, I. A. Drinnenberg2, E. P. Lei1; 1NIH/NIDDK, Bethesda, MD, 2Curie Institute, Paris, FRANCE.
11:30 am MS88 Distinct Roles of LINC Complex and Nucleoskeleton Components in Regulating Meiotic Chromosome Dynamics. C. Liu, Z. Lung, A. Dernburg; University of California, Berkeley and HHMI, Berkeley, CA.
11:40 am MS89 Identification of Regulators of Nuclear Shape. A. Schibler1, P. Jevtic2, G. Pegoraro1, D. Levy2, T. Misteli1; 1National Cancer Institute, NIH, Bethesda, MD, 2University of Wyoming, Laramie, WY.
11:50 am MS90 Multinucleation Associated Damage Promotes Quiescence Unlike Other Nuclear Atypia. M. Hart, V. M. Draviam; Queen Mary University of London, London, UNITED KINGDOM.
Room 147A
Moderator: Amanda S. Meyer, University of Southern California
11:00 am MS91 Membrane Homeostasis during Exocrine Secretion Is Maintained by Membrane Crumpling and Sequestration. K. Kumari, N. Scher, T. Biton, E. D. Schejter, B. Shilo, O. Avinoam; Weizmann Institute of Science, Rehovot, ISRAEL.
11:10 am MS92 Studying Dynamics and Mechanics of Clathrin-mediated Endocytosis in the Native Tissue Context. M. Mund, A. Picco, M. Kaksonen; University of Geneva, Geneva, SWITZERLAND.
11:20 am MS93 Expanding the Realm of Small GTPase Function: Evidence for Rab40b/Cul5 Mediated Rap2 Regulation during Cell Migration. E. D. Duncan, E. Linklater, R. Prekeris; University of Colorado Anschutz Medical Campus, Aurora, CO.
11:30 am MS94 Spontaneous Clathrin Lattice Curvature without Triskelia Replacement. B.
Heine, K. A. Sochacki, J. W. Taraska; National Institutes of Health, Bethesda, MD.
11:40 am MS95 ESCRT-III Heteropolymers Utilize Non-specific Lateral Interactions to Stabilize Curvature of Spiraling Polymers. S. Banjade, S. Tang, Y. Shah, S. D. Emr; Cornell University, Ithaca, NY.
11:50 am MS96 Neuronal Traffic Jams: Mechanistic Insights Into Mutant Mammalian Prion Aggregate-mediated Intracellular Transport Impairments. T. Chaiamarit, A. Verhelle, R. Chassefeyre, S. E. Encalada; the Scripps Research Institute, La Jolla, CA.
Room 146C
Supported by The Allen Institute
Moderators: Tim Fessenden, Massachusetts Institute of Technology, and Scott Wilkinson, National Institutes of Health
11:00 am MS97 Tissue Architectural Cues Drive Organ Targeting of Tumor Cells in Zebrafish. C. D. Paul1, K. Bishop2, A. Devine1, E. L. Paine1, J. R. Staunton1, S. M. Thomas1, J. R. Thomas1, A. D. Doyle3, L. M. Miller Jenkins1, N. Y. Morgan4, R. Sood2, K. Tanner1; 1Laboratory of Cell Biology, National Cancer Institute, Bethesda, MD, 2Translational and Functional Genomics Branch, National Human Genome Research Institute, Bethesda, MD, 3National Institute of Dental and Craniofacial Research, Bethesda, MD, 4National Institute of Biomedical Imaging and Bioengineering, Bethesda, MD.
11:10 am MS98 Live Cell Histology for Classification of Melanoma Cell Population Based on Single Cell Actions. A. Zaritsky1,2, A. R. Jamieson2, A. Nevarez2,3, E. S. Welf2, G. Danuser2; 1Ben-Gurion University of the Negev, Beer-Sheva, ISRAEL, 2UT Southwestern Medical Center, Dallas, TX, 3University of California San Diego, San Diego, TX.
11:20 am MS99 Multiplexed Fluorescence Lifetime Imaging Microscopy Reveals Dynamic Stem Cell Niche Metabolism in Lgr5-gfp Intestinal Organoids. R. Dmitriev, D. Papkovsky, I. Okkelman; University College Cork, Cork, IRELAND.
11:30 am MS100 Identifying Patterns of Protein Organisation in 3d Super-resolution Datasets. A. Curd1, R. Hughes1, A. Cleasby1, M. Baird2, Y. Takagi2, J. Ries3, H. Shroff2, M. Peckham1; 1University of Leeds, Leeds, UNITED KINGDOM, 2National Institutes of Health, Bethesda, MD, 3EMBL, Heidelberg, GERMANY.
11:40 am MS101 Single-molecule Imaging and Analysis of the Dynamic Organization of Vascular Endothelial Growth Factor 2 (VEGFR-2) on the Surface of Live Endothelial Cells.. B. Da Rocha-Azevedo1, S. Lee2, A. Dasgupta1, T. Kim1, M. Kittisopikul3,1, A. Vega1,4, L. De Oliveira1, K. Jaqaman1,4; 1Biophysics, University of Texas Southwestern Medical Center, Dallas, TX, 2Korea University, Seoul, KOREA, REPUBLIC OF, 3Cell and Molecular Biology, Northwestern University Feinberg School of Medicine, Chicago, IL, 4Bioinformatics, University of Texas Southwestern Medical Center, Dallas, TX.
11:50 am MS102 Waiting to Die: Nucleation-limited Signalosome Assembly Renders Human Cell Fate Decisions Inevitable. R. Halfmann1,2, A. Rodriguez Gama1, T. Kandola1, S. Venkatesan1, J. Wu1,2, M. Hu1; 1Stowers Institute for Medical Research, Kansas City, MO, 2University of Kansas Medical Center, Kansas City, KS.
Room 151B
Supported by The Allen Institute
Moderators: Amanda Haage, University of North Dakota; and Chen Kam, Yale University.
11:00 am MS103 Visualizing the Proliferation/differentiation Decision using A CDK Activity Sensor during Metazoan Development. A. Q. Kohrman1, R. C. Adikes1, J. J. Smith1, A. S. Aydin1, S. J. Collins1, O. Ahmed1, N. J. Palmisano1, C. Tian2, M. A. Martinez1, T. Medwig-Kinney1, S. Tank1, S. Liu3, W. Zhang1, R. A. Morabito1, N. Kim1, E. Feiner4, N. Weeks1, G. H. Thomsen1, T. Miller1, L. A. Davison5, S. L. Spencer2, B. L. Martin1, D. Q. Matus1; 1Stony Brook University, Stony Brook University, NY, 2University of Colorado, Boulder, Boulder, CO, 3University of California, Santa Cruz, Santa Cruz, CA, 4Princeton University, Princeton, NJ, 5University of Pittsburgh, Pittsburgh, PA.
11:10 am MS104 Definitive Cell Fate Identification Reveals Dynamic Growth in Oncogenic Clones. M. V. Sandoval1,2, S. Beronja2; 1University of Washington, Seattle, WA, 2Fred Hutchinson Cancer Research Center, Seattle, WA.
11:20 am MS105 Understanding Principles of Stem Cell Differentiation in the Regenerating Epidermis. K. Cockburn, V. Greco; Yale University, New Haven, CT.
11:30 am MS106 Isolation and Characterization of Putative Skeletal Stem Cells From the Effluent of Autologous Bone Graft Preparations. B. Le, A. Wessel, J. Florez, III, W. Yao, M. Lee, F. A. Fierro; University of California Davis, Sacramento, CA.
11:40 am MS107 Recapitulating Biological Signaling Scenarios with Spatiotemporal Control Using a Multiplexed, DNA-Patterning Approach. O. J. Scheideler, D. V. Schaffer, L. L. Sohn; University of California, Berkeley, Berkeley, CA.
11:50 am MS108 High-throughput Microfluidic Platform for in Vitro Vascularization of Human Organoids and Tissue Explants - Cell Cultures’ Missing Component?. D. Kurek1, S. Previdi1, F. Bonanini1, S. De Ruiter1, S. J. Trietsch1, A. Nicolas1, D. Hendriks2, D. Dutta2, H. Hu2, H. Clevers2, H. Lanz1, P. Vulto1, J. Joore1; 1MIMETAS B.V., Leiden, NETHERLANDS, 2Hubrecht Institute, Utrecht, NETHERLANDS.