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CellSlam 2007 Video & Winner Stephen Dahl

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CellSlam 2007 Winner

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Lentiviral Vectors By Stephen Dahl © 2007, All Rights Reserved

When I was a young man The plasmid was the king And with some lipofectin, you’d express most anything But some cells were refractile Transfection rates were poor And so we started making lentiviral-based vectors

Viral replication was sure to cause distress So we took out certain pieces and made plasmids of the rest But LTRs and integrase, we had to leave alone So lentiviral vectors could insert into genomes

Oh lentiviral vectors are based on HIV So lentiviral vectors should be handled carefully Biosafety cabinets, gloves, and labcoats with long arms Are what you should be using to protect yourself from harm

Now HIV binds T-cells with GP120 But in the viral vector its replaced with VSV-G VSV-G binds lipid and so it is clear to see The lentiviral host range growing exponentially

Yes, lentiviral vectors that use VSVG Can bind to phospholipid and get into you and me The LTRs and integrase are still inside your clones So lentiviral vectors can insert in your genome

Oh lentiviral vectors are based on HIV So lentiviral vectors should be handled carefully Biosafety cabinets, gloves, and labcoats with long arms Are what you should be using to protect yourself from harm

View CellSlam Movie (Quicktime,102mb)

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