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Protein Secretion and Vesicle Traffic Randy Schekman, March 2007 Department of Molecular and Cell Biology, HHMI, University of California, Berkeley

Lecture Overview Secretion mutants that block protein exit from the endoplasmic reticulum define genes involved in the formation, targeting and fusion of a small vesicle intermediate. SEC genes corresponding to the mutants defective in vesicle budding define the cytoplasmic machinery responsible for transport vesicle morphogenesis. A biochemical reaction that reproduces ER vesicle budding was reconstituted with gently-broken yeast cells and pure recombinant Sec proteins required in vivo for this budding event. The Sec proteins assemble on the ER membrane in the presence of GTP which activates a small GTPase, Sar1, initiating the formation of a coat protein complex called COPII.

Part 2: Biochemical Reconstitution of Transport Vesicle Budding (25:14) Click image above to start seminar in 320x240 format Click here to view in larger 640x480 format*   QuickTime 7 Required    *640x480 System Requirements (there may be a few seconds delay before seminars start) Downloads in alternative formats: (To Save, Right Click a link below and select "Save Target/Link As") QuickTime (46mb)     MP4 (295mb)     iPod/iPhone Video (295mb) PowerPoint Slides 1/page (1.6mb)     PowerPoint Slides 6/page (606kb) Format Descriptions Download Accelerator/Resume Broken Downloads Software

Part 1: Genetic Dissection of the Secretory Pathway (36:35)   Part 3: Human Diseases of Vesicle Budding (32:28) Speaker Bio    Related Articles

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