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Protein Secretion and Vesicle Traffic Randy Schekman, March 2007 Department of Molecular and Cell Biology, HHMI, University of California, Berkeley

Lecture Overview Protein secretion is executed by a cellular pathway involving the delivery of membrane and soluble secretory proteins in vesicles that capture newly-synthesized proteins assembled in the endoplasmic reticulum (ER) and sorted in the Golgi apparatus. Vesicles fuse with the plasma membrane resulting in the discharge of soluble molecules to the cell exterior and integration of vesicle membrane proteins and lipids in the cell surface. Baker's yeast cells grow by vesicle fusion and secretion at the tip of the daughter bud. A genetic dissection of this process was performed with temperature sensitive conditional mutants blocked at one of several stations in the secretory pathway.

Part 1: Genetic Dissection of the Secretory Pathway (36:35) Click image above to start seminar in 320x240 format Click here to view in larger 640x480 format*   QuickTime 7 Required    *640x480 System Requirements (there may be a few seconds delay before seminars start) Downloads in alternative formats: (To Save, Right Click a link below and select "Save Target/Link As") QuickTime (68mb)     MP4 (428mb)     iPod Video (428mb) PowerPoint Slides 1/page (2.6mb)     PowerPoint Slides 6/page (964kb) Format Descriptions Download Accelerator/Resume Broken Downloads Software

Part 2: Biochemical Reconstitution of Transport Vesicle Budding (25:14)   Part 3: Human Diseases of Vesicle Budding (32:28) Speaker Bio    Related Articles

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