A number of rare diseases, including Emery-Dreifuss muscular dystrophy and Hutchinson-Gilford progeria syndrome, are linked to the disruption of nuclear envelope transmembrane (NET) proteins. However, it has been difficult to determine the location and translocation rates of NETs in the outer and inner nuclear membranes. Now ASCB member Krishna Mudumbi, and colleagues Eric Schirmer and Weidong Yang at Temple University and the University of Edinburgh, have established a single-point single-molecule FRAP microscopy technique to determine the localization and translocation rate of NETs in living cells.
